EXPT NO.

11 30
PAPER CHROMATOGIRAPHY
PRINCIPLE:
Papu cksomatognabAy has buw su s tu met pobulas
chuoratograhl~ peecaelunat au 24 daplese ane.
Oaher chuomatcgabhy & a lorm a patitian hronmakograbhy
wh a r t a solts is deharatd a a
kut e
hapen. o u antitanin Lnvolve i tA. shasatiea
Cembourd haben ekuemateguebhy, it, liqil -Liquil
hartitiow a loll -

Liauil aniiliow
L l -Lauid partitia
kiquid quld farti tion
taks placs belsn thi satiouauy
aguou}hasu (duu ts watn moleculs Lightly boundko
cellees Libens) thpapr and . oganie
u. obil. oHaai
024ed lase basse
cabtllay acte duwring h 4parataow bueces
2) Alid-liquisl pautibon
SstA - liapiol ard'tbws dus to adsonpton of sol.ta
celllosa ibnee ank dconftion letatiu)'f t
solug by. H samu bile oHgauie phate desin th
sepasakia aoces.
be
Jhrle, fabs chismatoquablic teckaias can ueel
aich a 4olste fot
o saharetan hne yoluts,
w a t as i 04qe lokrus.
 PROCEDuRE

Choie hapers
hatwauw No. I on 3 Ltu papons ul
a
kon babex
CKOato9aphy .
whalunan ale .! fo Smalln quanlite a
hatmau Ato3 aA kangu uantitiu a e d& ( bod r
uolutiow bettu. akuman
Alo. ahos). Jh bafn isaut
quined áge eknauafoguahlio tark, Aatenalia
w a u e o fhase anl ain uid pe betton auolulin!
L i wil a faael d n a abot 2 CM r a Ha bottom.
dampla au ahtid a a unall ahoB uking a cotlla
enl L and drid. Dthull tanltu ean
be khottd sami hofe i a ditane obant +5om
harween hem

Solyant dyttem
seuew dyste asuall cenbored
Ghu an ougAie solvet
saturatal watw. Sixu th ph
2nbondart on bettes kusolulieu 4 cenbounks, h tlorak
syslems cond ain Jmall amaurk aceue ail o4 amwoutun

ydroxide o ciati aidie e kaie enwikauneak. r

an

counbonenli lh selwert st a wined u l l Chuct

buobontie shakn and t

ll hhass a allewrdl t
sepasat. d lhr oponie slatt {itud kLaangl kv
layut kLtu pepu do emove Ausbnde usotu ducfut
a akan nto t chrematoguablie tanl. kanA
igkty elosd fo 3-4 hu,e ntnu coslk taliualio
 32

holvert systemt kon ehu ekaouategudh,

.Budanel cti add | wJa CA0:

(0: 58)
2.
n-Butaned [ Pyuidi | wata
0 (33:33: 33
3. AhaedPyaidi| wetu
(5:12: 63
ON Meno and diuaceharicdu

Butauel [ Pyuidis | Watu (s0: 2:32)

2n-Butaur Hand| watu 52:3315
Hon Chluohhyl and
canstensiks
.-faehaneh | Petudeum thu (4:96)
2 tonofaa ( PBuoleus. ku (30:70)

dehanatiow Campounds
Ale bottom dg t hoper , *lem cauful

did in selvet (Hak tuu h shot do net louch

th selven diuray). Jhu uobilL shue uov4 up en
apu hnovg cof?lay actio Ouning movement
liguid- l uid and olid -liauid haxteianinq, oceu
tapeakrtly as h ekvent faont adwancu a ya Alts
a tsobed a cconeling é heu faultiainp coekticiente
AS CENDIaIG TYPE DEsCENDIAlG TYPE
 33
dte abot 6hx (i.t), until Aolvot
hand kas movd
about 2cm om top ge papu, l paba
Aemoved h sel vent fhant i masked
ahencel ad ai drid kos to- 5 min. Jhis the
uoatoa
cluomatoguosly khows ascling chuo mato gsa
descaading comatoguapl mehile haie
mo ve towasds (downasael
l t as dewn i f kg (Juo diuensional fabe
chromat egnapl On TLC sik t
ailnt telvert ayzks
nnd ucLbetta kesdatin, spedally dhen Hicku
walk Apotu
hok
Lo concednatiou

dectraw Comfounds
hcompou nds au deleotd i a by bheyionl o alanlcal
mlans Auino aids a debelid uing rinhadnin (o.2.
acutane) ILL inkydkia lolutiow accta snad
and hiacd a at 60 C util
ovan
{inst %Las on
uupte bot i tan and hen stoud A.
ckuomalaguaphic
ismade , as A sholi wil kade away fe .ta
detetio hagau, chumakoguae habu
alkaln Alva afhati solatiow and dhleo
apel
u
dsuihl 6elos.
Canbaty drate skaiig
. 4hter developmenk, aii -d B ekrouatognan
22 0: h pahn ke ainuki a utray eortaining
200 ml aestont and Inl sat. touti Aa dia-dy
3. 9th haber fok 5 in 200 l d 5. Aodism (3A)
iasulpka oletron to stob £A keactow aud cleas Hir
bakanound .

R and ualuu

denotu HRalati front 4 h f a l and t solvent

A katio dutance moved y telvtnk t h
distance mov eol £A solule and alray kuu kon 1
OR 1 ( i .e),

Siatance moved bu th sobstana

Distanu Auered solweak

h
Kvalus chanactudste 8a combound eundes a sk 4
ane

npumertal conditious valuu Cannot le toansl et on

ducudin 4 4kuomatagaasly, a
thi solweut ent
alusasovu ouk h hapa. s du casel R valuts
ar udd al kees ace
Ditane anoved subskauce
Oiutana moved

Aesul 4 a
babu ehnonmatoguaphs xhvnat anio aciol
Oinelve ti tollaari aino asid at otmglme dd sat
( mM KOA
G, Pre, Hü, Tyn, Me, Gu, Ang ard La

Wa diuelve yn ad Gtu)
 35
-
Bukand Acdi acid kata (4:1:5). Mix hu cautpouuk
and hake aelli a
Atharati un ad allos ta phasu
dehanat. Jranttu H uphu watu Aakualid bekanel

hotI A 4 anino acid

samlu uning a cap?lay
and duks hhoba o 6ku in a abon selvnk
equiinetrd chanhu uequl LPhatid fo ~skai)
Dn olveh kond movd 23
babukemovrd, Lh holvud rort
masked tmnmediatel
apuil aud ein . drid pk toia. hu bahm
shna d i o-2Y. nhydri a c t o ad lacubatl
a Lot u ovn o 5./0 h util he
Meti abbas.