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    Bacterial Concrete

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    Vetri Vel
    This document summarizes research on using bacteria to improve the durability of concrete. Bacillus pasteurii was used, as it can continuously precipitate calcite. Different concentrations of bacteria suspended in water, urea, or phosphate buffer were tested on concrete specimens. Phosphate buffer proved the most effective medium for bacterial growth. Specimens treated with bacteria suspended in phosphate buffer showed increased resistance to alkali, sulphate, freeze-thaw cycles, and shrinkage compared to untreated concrete. Compressive strength also increased by 5-10% with bacterial treatment.

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    Bacterial Concrete

    Uploaded by

    Vetri Vel
    609 views19 pages
    This document summarizes research on using bacteria to improve the durability of concrete. Bacillus pasteurii was used, as it can continuously precipitate calcite. Different concentrations of bacteria suspended in water, urea, or phosphate buffer were tested on concrete specimens. Phosphate buffer proved the most effective medium for bacterial growth. Specimens treated with bacteria suspended in phosphate buffer showed increased resistance to alkali, sulphate, freeze-thaw cycles, and shrinkage compared to untreated concrete. Compressive strength also increased by 5-10% with bacterial treatment.

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    This document summarizes research on using bacteria to improve the durability of concrete. Bacillus pasteurii was used, as it can continuously precipitate calcite. Different concentrations of bacteria suspended in water, urea, or phosphate buffer were tested on concrete specimens. Phosphate buffer proved the most effective medium for bacterial growth. Specimens treated with bacteria suspended in phosphate buffer showed increased resistance to alkali, sulphate, freeze-thaw cycles, and shrinkage compared to untreated concrete. Compressive strength also increased by 5-10% with bacterial treatment.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    609 views19 pages

    Bacterial Concrete

    Uploaded by

    Vetri Vel
    This document summarizes research on using bacteria to improve the durability of concrete. Bacillus pasteurii was used, as it can continuously precipitate calcite. Different concentrations of bacteria suspended in water, urea, or phosphate buffer were tested on concrete specimens. Phosphate buffer proved the most effective medium for bacterial growth. Specimens treated with bacteria suspended in phosphate buffer showed increased resistance to alkali, sulphate, freeze-thaw cycles, and shrinkage compared to untreated concrete. Compressive strength also increased by 5-10% with bacterial treatment.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 19

    Presented by U.VETRIVEL Arunai College of Engineering Tiruvannamalai.

    Bacillus Pasteruii, a common soil

    bacterium, can continuously precipitate


    calcite under favourable conditions .This phenomenon is called microbiologically induced calcite precipitation. Due to its inherent ability to precipitate

    calcite continuously bacterial concrete


    can be called as a Smart Bio Material.

    To study the effect of different concentrations of bacteria on the durability of concrete.

    To study the efficiency of bacteria when suspended in different mediums (water, phosphate and urea)

    A stock culture of B.pasteurii is generally maintained in a solid medium containing:


    10g trypticase
    5g yeast extract 4.5g tricine 5g ammonium sulphate 2g glutamic acid

    And final concentration of 1.6% agar, which is


    autoclaved separately and added after-wards.

    Microbiologically enhanced crack remediation (MECR) utilizes a biological by-product, CaCO3.

    The overall chemical equilibrium reaction of calcite


    precipitation is,

    (Ca2++ CO32- CaCO3)


    Ca2+ + Cell Cell-Ca2+ . . . . (1) Cl- + HCO3- + NH3 NH4Cl + CO32- . . (2) Cell-Ca2+ + CO32- Cell-CaCO3 . . . (3)

    The effects of the following parameters on the

    durability of concrete were investigated:

    Bacteria suspended in water (BW). Bacteria suspended in urea-CaCl2 (BU). Bacteria suspended in phosphate buffer (BP)

    As per IS456:2000 the following are the some of the important factors which affect the durability of concrete

    * Impermeability

    * The environment
    * The type and quality of constituent materials * The water/cement ratio of the concrete * compaction and efficient curing

    Cube drilled to a depth of 2 CM

    Testing of Cubes sealed with the bioconcrete

    Description of specimen Control (concrete without crack)

    Strength N/mm2 33.33

    Cracked specimen
    Remediated specimen

    26.66
    30.22

    More resistance to the freezing and thawing

    condition
    Reduce plastic shrinkage cracks High impermeability High resistance to chemical attacks

    The presence of bacteria in different mediums increased the resistance of concrete towards alkali, sulphate, freeze-thaw attack and drying shrinkage. Phosphate-buffer proved to be an effective medium for

    bacteria than the other two mediums


    The compressive strength of bacterial concrete is also increased by 5% to 10%.

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