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    Artificial Insemination in Dogs

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    sherinshahs2010
    Artificial insemination in dogs involves collecting semen from a male dog, evaluating the semen quality, and then artificially inseminating a female dog. There are three main steps - semen collection, semen evaluation, and artificial insemination. Semen can be used fresh, chilled, or frozen. The document outlines the various methods for collecting semen from male dogs and inseminating females, including vaginal and intrauterine insemination techniques. Artificial insemination is useful for breeding programs by maintaining good genetics, eliminating disease transmission, and allowing use of genetics from dogs that have died. However, it requires expert knowledge and success rates are generally around 65-85% with small

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    Artificial Insemination in Dogs

    Uploaded by

    sherinshahs2010
    132 views18 pages
    Artificial insemination in dogs involves collecting semen from a male dog, evaluating the semen quality, and then artificially inseminating a female dog. There are three main steps - semen collection, semen evaluation, and artificial insemination. Semen can be used fresh, chilled, or frozen. The document outlines the various methods for collecting semen from male dogs and inseminating females, including vaginal and intrauterine insemination techniques. Artificial insemination is useful for breeding programs by maintaining good genetics, eliminating disease transmission, and allowing use of genetics from dogs that have died. However, it requires expert knowledge and success rates are generally around 65-85% with small

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    Artificial Insemination in Dogs

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    Artificial insemination in dogs involves collecting semen from a male dog, evaluating the semen quality, and then artificially inseminating a female dog. There are three main steps - semen collection, semen evaluation, and artificial insemination. Semen can be used fresh, chilled, or frozen. The document outlines the various methods for collecting semen from male dogs and inseminating females, including vaginal and intrauterine insemination techniques. Artificial insemination is useful for breeding programs by maintaining good genetics, eliminating disease transmission, and allowing use of genetics from dogs that have died. However, it requires expert knowledge and success rates are generally around 65-85% with small

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    132 views18 pages

    Artificial Insemination in Dogs

    Uploaded by

    sherinshahs2010
    Artificial insemination in dogs involves collecting semen from a male dog, evaluating the semen quality, and then artificially inseminating a female dog. There are three main steps - semen collection, semen evaluation, and artificial insemination. Semen can be used fresh, chilled, or frozen. The document outlines the various methods for collecting semen from male dogs and inseminating females, including vaginal and intrauterine insemination techniques. Artificial insemination is useful for breeding programs by maintaining good genetics, eliminating disease transmission, and allowing use of genetics from dogs that have died. However, it requires expert knowledge and success rates are generally around 65-85% with small

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 18

    DEPARTMENT OF ANIMAL REPRODUCTION GYNECOLOGY AND OBSTETRICS

    by Nirmal Chacko 2006-03-17

    What

    is artificial insemination? Frist in dogs Spallanzani In dogs AI is limited


    1.
    2.

    cases where copulation is not possible geographically remote sires and bitches

    3 steps Semen collection Semen evaluation Artificial insemination

    Male

    used - called stud 2 methods of collection 1. Digital manipulation and massage 2. Using an artificial vagina

    Stimulation of male 1. Bitch in heat 2. Vaginal swab from oestrus bitch

    Once dog mounts a. Grasp prepuce and expose tip of penis. b. Slide collection cone over the protruding penis. c. Hold bulbus glandis, apply pressure. d. Continue to apply pressure until a crystal clear fluid flows into collecting vial.

    Semen has 3 fraction 1. Pre sperm fraction- slightly cloudy, 1-3 ml 2. Sperm rich fraction- milky, 0.1-6ml 3. Prostatic fraction- clear, transparent, variable volume Examine volume, colour, pH, progressive motility, concentration and total no. of spermatozoa, morphological abnormalities 70% progressive motility

    AI may involve use of a. Freshly collected semen b. Semen that has been diluted and chilled c. Semen that has been frozen and thawed Two categories 1. Vaginal insemination 2. Intrauterine placement of semen 150 million motile spermatozoa

    For

    fresh and chilled semen Semen placed in the vaginal canal of bitch at the level of external os Total volume of semen- 2 to 4ml

    For

    chilled and frozen semen Several methods


    a.

    a.Transcervical insemination- use of catheters


    foley catheter, Norwegian/Scandinavian catheter, endoscopic technique b. Surgical intrauterine deposition- laparoscopy, full abdominal surgery

    b.

    Animal

    under general anaesthesia Full abdominal surgery- midventral incision to expose uterus-semen directly introduced into uterus using needle attached to catheter Laparoscopy- acceptable than full abdominal surgey

    Various methods 1. Fresh and cooled to room temperature 2. Diluted and cooled to 50C 3. Diluted and frozen (-1960C) Lifespan spermatozoa in fresh semen 4hours Chilled semen - 2 to 3days Frozen semen - indefinite

    Simple

    diluents Skimmed milk- heated to 92-950C for 10 mts Egg yolk Semen : extender 1:4 Slow cooling to 50C Double insemination of same bitch Cranial vaginal or intrauterine insemination

    Process of freezing is same as that in bovines Earlier- as pellets on dry ice Liquid nitrogen Extender- Tris, fructose, citric acid, water, glycerol and egg yolk along with antibiotics 0.5ml straws Thawing- 370C for 30-90 secs or 750C for 6 secs Frozen dog spermatozoa has short post thaw lifespan

    Maintain and develop breeds with good genes Elimination of diseases spread by contact Useful when natural mating is difficult Economic and transportation of animals can be avoided Frozen semen-use of genetic material even after death of a valuable stud Elimination of undesirable characteristics from a breed

    Need expert knowledge and skill Selective breeding result in selection for or against many other character Disease transmission to females through contaminated semen Success rate is low,65-85% and small litter

    History

    of dog AI dates back to 18th century Limited use Useful tool in improving overall quality of dog breeds Elimination of undesirable characteristics

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