Plasma Proteins
Plasma Proteins
Plasma Proteins
ELECTROPHORETIC MOBILITY
• Paper Electrophoresis
Filter paper
Perspex chamber
Phenobarbitone Buffer – pH 8
Electric current - 18 Hrs.
Paper kept at 110 ° C
Stained – Sudan Black/Light
Green
Measure density - Densitometer
6. BUFFER ACTION
Plasma Proteins has 1/6 of Total Buffer Power of blood.
7. MOLECULAR WEIGHT
ALBUMIN - 69 000 Da (Dalton)
β GLOBULIN - 90 000 Da
γ GLOBULIN - 156 000 Da
FIBRINOGEN - 330 000 Da
9. VISCOSITY
Resistance offered by it’s own molecules against it’s flow.
Asymmetrical molecule - More Viscosity
Symmetrical molecule - Less Viscosity
I. LIVER
Liver is the main site of formation of plasma proteins.
ALBUMIN
FIBRINOGEN
α1 GLOBULIN
α2 GLOBULIN
β GLOBULIN
3. MAINTANANCE OF VISCOSITY
OF BLOOD
Globulin (Mainly)
Fibrinogen
4. BUFFER ACTION
Can accept or release H ions
5. ANTIBODIES
γ Globulins
Agamaglobunaemia
Infections
6. ROULEAUX FORMATION
Fibrinogen
7. TRANSPORT OF SUBSTANCES
α1 GLYCOPROTEINS
I. Thyroxine binding protein - Thyroxine
ii. Transcortin - ACTH
α2 GLYCOPROTEINS
i. Ceruloplasmin - Copper
ii. Hepatoglobulin - Haemoglobin
β GLYCOPROTEINS
Transferrin - Iron
8. PROTEIN RESERVES
Tissue proteins
HYPOALBUMINEMIA - Plasma Albumin
CAUSES : -
1. NUTRITIONAL DEFICIENCY
2. DEFECTIVE INTESTINAL ABSORPTION
3. DEFECTIVE SYNTHESIS -
Liver Diseases Plasma Protein level
4. INCREASED LOSS OF PROTEINS
Nephrotic Syndrome
Severe Burn