Physical and Behavioral Development of Laboratory Rats On Modified Conditions

Physical and Behavioral
Development of
Laboratory Rats on
Modified Conditions
Proponents:
Camille B. Gabriel
Jean Allen Layderos
Clariz D. Libario
Shiela Mae C. Melarpisa
Geriel Jake Ojano
Maria Eloisa Pesigan
Vhincent Axl E. Villareal
Introduction
I. Behavioral development
a. an essential survival tool
b. is influenced by multiple environmental factors
c. should meet one or more of three criteria
II. Physical development
Background of the Study
 This study was conducted on the Southern Luzon State University
that aims to determine the changes that will happen to the
experimental organism(laboratory Rats)by modifying their
environment.
 The rats were bought on the Apostol Pet shop in Lucban, Quezon.
Both rats are 1 month old with initial weight of 31 grams (male) and
34 grams (female). The female rat has a white fur with a black spot on
her head while the male rat has a pure white fur.
 The modified environment includes, the changes in matt color to
black colored paper and feeding Fita Crackers for 60 days.
Conceptual Framework
Behavioral Psychology
 a theory of learning based on the idea that all behaviors are
acquired through conditioning. Conditioning occurs through
interaction with the environment.
 Behaviorists believe that our responses to environmental
stimuli shape our actions.
 behavior can be studied in a systematic and observable
manner regardless of internal mental states.
Research Paradigm
INPUT PROCESS OUTPUT

-feeding the Info-graphic
-30 grams of rats once a day
fita about the
-creating a physical and
-20 ml of diary or day to
water -black behavioral
day development
colored paper observation to
(matting) of the rats.
the rats
Statement of the Problem
This research study aims to:

• To determine physical development of the laboratory rats in terms of:
a) Weight
b) Length
• To find out the behavioral development of the laboratory rats.
• To establish and discuss the effect of the modified condition applied on the rats:
a) Food
b) Environment
c) Matting
d) Number of offspring
Scope and Delimitation
This study focuses
 On the physical and behavioral development of two (2)
laboratory rat under modified condition.
 How the modified condition (food, mating, and environment)
will affect the development of the lab rats in terms of both
physical and behavioral aspects.
 The study only limits to the observed changes of the
researchers that will be obtained from the interpretation of
written numerical data of the researchers.
 To obtain the numerical data related to the physical
development of the laboratory rats and descriptive data
related to the behavioral development of the experimental
variable.
• To evaluate and interpret the obtained data to determine the
effects of the modified conditioned applied on both physical
and behavioral.
• To discuss the effects of being under modified conditions and
the factors that affects the variables to change.
Methodology
ANIMALS
• Both the male and female are
laboratory mouse with the initial
weight of 31 grams and 34
grams.
• Male rat has pure white fur
• Female rat has a white fur with
a black spot
CAGING
 At the age of three weeks both
the male and female were
housed in a plastic base-
aluminum cage.
 The cage floor was occupied

by black colored papers that
were regularly changed every
day.
DIET
 The food supplemented each day
were ten grams of Fita Biscuits
and twenty ml of water around
twelve o’clock in the afternoon.
Research Design
 Experimental and descriptive design
 The results obtained for the study are based
through;
 Observation
 data recorded by the researcher in the daily journal
Data Gathering Procedure
 The experiment was a paired-housing and feeding to
let the mouse copulate
 Mice were weighed and length measured weekly
 behavioral observation were recorded daily in the
journal to be used later on the study
Research Instruments
 Weighing Scale (Analog)
 Ruler and Tape Measure
 Daily Records/Journal
Data Analysis Plan
 In terms of the quantitative data, the sum body weight and length of
both the mouse is recorded before the diet is given (initial weight,
IW), (initial length, IL) and then at the termination of the experiment
(final weight, FW), (final length, FL). Comparison of the mean
weight gain will test if changes have occurred; compare mean weight
gain by the usual unpaired X-test, where weight gain is computed by
(FW-IW) and length gain is computed by (FL-IL).
 Additionally, a descriptive approach is used in showcasing the data
gathered by observation in discussing the effect of the modified
condition in terms of the food, environment, matting and number of
offspring.
Figures
Physical Development of rats in terms of Weight
Weight of Laboratory Rats
45
40
35
30
25
20
15
10
0
Day 1 Day 30 Day 60
Male Female
Figure 1.1 The Weight of the Laboratory Rats during the 60-day experiment
Physical Development of rats in terms of Length of Body and Tail
Length (body) of Laboratory Rats

12
10
0
Day 1 Day 30 Day 60
Male Female
Figure 1.2 The length (body) of the Laboratory Rats during the 60-day experiment
Length (tail) of Laboratory Rats
10.5
10
9.5
8.5
7.5
Length 1 Length 2 Length 3
Male Female

Figure 1.3 The length (tail) of the Laboratory Rats during the 60-day experiment
Findings
Physical Development of rats in terms of Weight:
 The rats gained weights in the first 30 days

Male - 8 grams (from 31g to 39g)
Female- 3grams (from 34g to 37g)
 After 30 days, the rats lose weights
Male - 5 grams (from 39g to 34g)
Female- 8grams (from 37g to 29g)
Physical Development of rats in terms of Length of Body
and Tail
• The length of the body and tail of the rats increased in the first
30 days.
Male’s body- 1cm (from 9cm to 10cm)
tail- 1.5cm (from 8.5cm to 10cm)
Female’s body- 1cm (from 10cm to 11cm)
tail- 0.5cm (from 8.5cm to 9cm)
• After 30 days, the length of body and tail of the rats retained.
Number Offspring
 The rats do not produce any offspring within the 60-day
experimentation.
 Causes:
-Stress
-Do not get enough nutrients
-Lack of Protein intake

Behavioral Developments of Laboratory Rats
 Aggressive at first day but behave on the following days

 The male rat is more energetic than the female but as day
passes by male rat became less active than the female.
 They slept more on day and more active at night
 They liked to cuddle with each other and they liked to be on
the exercising wheel.
 The female rat liked to wash her face with water.
 They eat using their paws .
 They gnaw their food and drinking container, also the
plastic part of the cage.
 The male rat moves circularly when put outside the cage
while rotating his head and when held, the male rat was
shaking.
 Both of the Laboratory rats doesn’t go anywhere far from
the cage whenever the researchers open the cage and let
them go outside, instead, they just go near the cage or
climb outside their cage.
Effect of food on the Rat
 The average amount of food that the rats

consume in a day was 7 grams on the first 15
days, 5 grams for the next 15 days and 7
grams for the rest of the experiment days.
 The rats suffered from sudden weight loss;
the female was more affected.
Effect of Environment on Rats
 They are more active when there is no light.

 They play more when the piece of plywood
that serves as slide were placed inside the
cage.
 They liked to climb and hang on the stick that
were stuck on the cage.
Effect of Black Matting on Rats
 Color of the environment plays a vital role in the
development of the behavior of the rats.
 Change in behavior due to black matting can be observed:
- they became more sleepy
- lazy
- less active
Conclusion
 Foods play an important role in the body, especially in the
development of the physical and behavioral aspect of the rats.
 The researchers also noticed that rats who are fed by Fita have
shorter body and low weight as compared to their normal
growth.
 Another factor that contributes to the behavioral development of
the rats is their environment.
 The black colored paper can affect them in terms of the sleeping
habit because the rats fall asleep often even at day time.
 The rats are often listless and sleeping.
 Tight cage contributes to the formation of the unusual
behavior of the male rat ( rotating his head and making
circular movements when released in the cage)
 To develop the physical and behavioral aspects of the
laboratory rats, they should be given adequate foods that
contains proteins, fats and vitamins not only
carbohydrates
 Any modification applied on rats does not only affect its
physical and behavioral development but also the
reproduction of the rats.
Recommendation
 Increase the awareness on proper caring of the
laboratory rats. In terms of foods, environment and
matting.
 Provide more scientific experiment and use device to
support the data collected.
 Consult to a veterinarian to strengthen the result and
data that has been collected.
Appendixes
Usual behavior of rats in the morning
(Sleeping)
The weights of the rats in the first 30 days
The lengths of the rats (body and tail) in the first 30 days
Unusual Behavior of the Male rat
Petting the rat to notice unusual (aggressive) behavior
Letting the rats go out of the cage
Weekly play of the rats outside
The rats after 60 days of experimentation and
observation

Physical and Behavioral Development of Laboratory Rats On Modified Conditions

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Physical and Behavioral

INPUT PROCESS OUTPUT

This research study aims to:

 The cage floor was occupied

Length (body) of Laboratory Rats

 The rats gained weights in the first 30 days

 Aggressive at first day but behave on the following days

 The average amount of food that the rats

 They are more active when there is no light.

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