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    5.2 - Effects of Substrate and Enzyme Concentration

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    yessirrrr
    Higher substrate concentration increases reaction rate by providing more molecules that can bind to enzyme active sites, until enzymes are fully saturated. Higher enzyme concentration also increases reaction rate by providing more active sites for substrate to bind, doubling reaction rate when enzyme amount doubles, until all substrate is consumed and its concentration limits the reaction.

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    5.2 - Effects of Substrate and Enzyme Concentration

    Uploaded by

    yessirrrr
    25 views2 pages
    Higher substrate concentration increases reaction rate by providing more molecules that can bind to enzyme active sites, until enzymes are fully saturated. Higher enzyme concentration also increases reaction rate by providing more active sites for substrate to bind, doubling reaction rate when enzyme amount doubles, until all substrate is consumed and its concentration limits the reaction.

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    Higher substrate concentration increases reaction rate by providing more molecules that can bind to enzyme active sites, until enzymes are fully saturated. Higher enzyme concentration also increases reaction rate by providing more active sites for substrate to bind, doubling reaction rate when enzyme amount doubles, until all substrate is consumed and its concentration limits the reaction.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    25 views2 pages

    5.2 - Effects of Substrate and Enzyme Concentration

    Uploaded by

    yessirrrr
    Higher substrate concentration increases reaction rate by providing more molecules that can bind to enzyme active sites, until enzymes are fully saturated. Higher enzyme concentration also increases reaction rate by providing more active sites for substrate to bind, doubling reaction rate when enzyme amount doubles, until all substrate is consumed and its concentration limits the reaction.

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    © All Rights Reserved
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    The Effects of Substrate Concentration

    If the enzyme concentration is fixed:


    • The higher the concentration of substrate, the higher the rate of
    reaction as more substrate molecules bind to the active site of
    the enzymes to form the enzyme-substrate complex.
    • The enzyme molecules are saturated with substrate. The excess
    substrate molecules cannot react until the substrate already
    bound to the enzymes has reacted and been released.
    • Therefore, at the maximum level, the concentration of enzymes
    becomes a limiting factor.
    The Effects of Enzyme Concentration
    If the substrate concentration is fixed:
    • The higher the concentration of enzymes, the higher the rate of
    reaction as there are more active sites for substrate molecules to
    bind to and form the enzyme-substrate complex.
    • If the concentration of an enzyme in one reaction is doubled, the
    amount of substrate converted to products per-unit of time is also
    doubled.
    • The rate of reaction will decrease when all substrate is used up
    and can only be increased by adding more substrate.
    • Therefore, at the maximum level, the concentration of substrate
    becomes a limiting factor.

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