Scribd Logo
Upload

Welcome to Scribd!

  • 2 views

    Sakshi Ppt[1]

    Uploaded by

    akshata0809

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    Sakshi Ppt[1]

    Uploaded by

    akshata0809
    2 views20 pages

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    2 views20 pages

    Sakshi Ppt[1]

    Uploaded by

    akshata0809

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 20

    RIBOSOMES, THEIR

    DISSOCIATION
    AND SELF ASSEMBLY

    Presented By : Sakshi Singh Guided By : Dr. Neeshma Jaiswal


    CONTENTS
    • WHAT ARE RIBOSOMES
    • DISCOVERY
    • OCCURRENCE
    • ORIGIN
    • TYPES
    • FREE RIBOSOMES VS BOUND RIBOSOMES
    • PROKARYOTIC VS EUKARYOTIC RIBOSOMES
    • SVEDBERG UNIT
    • DOMAINS OF RIBOSOMES
    • SITES OF RIBOSOMES
    • RIBOSOMES ASSEMBLY
    • FACTORS AFFECTING RIBOSOMES ASSEMBLY
    • RIBOSOMES DISASSEMBLY
    WHAT ARE RIBOSOMES?
    s have tiny granular structure known as ribosomes.

    somes are ribonucleoprotein particle

    somes serves as workbenches, with mRNA acting as blueprint in the process of p


    thesis
    DISCOVERY

    e ribosomes are first noted in plant cells by


    obinson and brown in 1953
    ortly afterward Palade in 1955 observed them in
    nimal cells
    isolated the ribosome and detected the RNA in
    em in 1956
    e nobel prize in chemistry 2009 was awarded to
    enkatraman Ramakrishnan, Thomas steitz and Yonath
    r determining the detailed structure and mechanism
    the ribosome
    OCCURENCE

    • Ribosomes occurs in both


    eukaryotic and prokaryotic cells
    • In prokaryotic cells ribosomes
    occurs freely in the cytoplasm
    but in eukaryotic
    cells attached to the outer
    surface of the
    membrane of endoplasmic
    reticulum
    ORIGIN
    IN PROKARYOTES

    3s, 16s and 5s rRNA are transcribed by specific segment of nucleoid


    3s and 5s rRNA associates with cytoplasmic protein to form 50s subunit
    6s rRNA along with their proteins form 30s subunit

    IN EUKARYOTES

    Nucleolar organizing region transcribes 45s nucleolar RNA(precursor


    of 5.8, 28, 18s rRNAs)
    5s rRNA is transcribed from the rDNA lying adjacent to NOR
    TYPES
    On the basis of location

    Free ribosomes Bounded ribosomes

    On the basis of sedimentation coefficient

    70s ribosomes 80s ribosomes


    FREE RIBOSOMES VS BOUND RIBOSOMES
    SVEDBERG UNIT

    he large and small subunit of ribosome are named according to the ve


    edimentation when subjected to centrifugal forces
    he unit used to measure the sedimentation velocity is Svedberg
    he sedimentation velocity is a function of a particles molecular weight
    olume and shape
    DOMAINS OF RIBOSOMES

    There are two domains of ribosomes

    TRANSLATIONAL DOMAIN
    • The region responsible for translation is called the
    translational domain
    • Both subunits contribute to this domain , located in the
    upper half of the small subunit and in the associated areas of
    the large subunit

    EXIT DOMAIN
    • The growing peptide chain emerges from the large subunit at
    the exit domain
    • This is located on the side of the subunit
    SITES OF RIBOSOMES

    The ribosomes has three sites for binding


    tRNA

    • P site – The peptidyl or donar site

    • A site – The aminoacyl or acceptor site

    • E site –The exit site


    ASSEMBLY OF RIBOSOMES
    ASSEMBLY IN EUKARYOTES
    FACTORS INFLUENCING RIBOSOME ASSEMBLY

    nvironmental Factors : temperature and ion concentration

    utation : can disrupt ribosome assembly, leading to diseases as ribosomopathi

    nergy : ATP and GTP are required for ribosome assembly and function
    DISASSEMBLY OF RIBOSOMES
    RIBOSOME DISSOCIATION AND RECYCLING

    ation after translation : Ribosome recycling factor and GTPase help split the subunits

    ng process : Release of mRNA and tRNA , subunits separate and are ready for another round of
    RIBOSOMES ASSEMBLY AND DISASSEMBLY
    THANK YOU
    SAKSHI SINGH

    You might also like

    pFad - Phonifier reborn

    Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

    Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


    Alternative Proxies:

    Alternative Proxy

    pFad Proxy

    pFad v3 Proxy

    pFad v4 Proxy