Recombinant dna technology

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Process of Recombinant DNA Technology is the technique used for making artificial DNA by genetic modification using multiple sources. Process of Recombinant DNA Technology involves extraction of gene followed by cleaving it at special sites. Then it is put in a molecular vector for its setup in host. At last we need an expression system on which vector along with gene of interest is introduced for multiple copies.

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Ethanol precipitation of DNA with ice-cold ethanol or isopropanol. The ionic strength of sodium acetate can be used to improve precipitation. The precipitated DNA appears as threads in the final solution. Phenol-chloroform extraction can also be used to separate DNA from proteins. Another method to extract DNA is the minicolumn purification. Recombinant Dna Technology, Dna Ligase, Dna Extraction, Dna And Rna, Recombinant Dna, Dna Polymerase, Dna Technology, Plasma Membrane, Dna Molecule

Ethanol precipitation of DNA with ice-cold ethanol or isopropanol. The ionic strength of sodium acetate can be used to improve precipitation. The precipitated DNA appears as threads in the final solution. Phenol-chloroform extraction can also be used to separate DNA from proteins. Another method to extract DNA is the minicolumn purification.

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