Biochem Case Problems 02

Case 2
Histidine-Proline-rich Glycoprotein as a Plasma pH Sensor

Focus concept
A histidine-proline-rich glycoprotein may serve as a plasma sensor and regulate local pH in extracellular fluid during ischemia or metabolic acidosis.
Prerequisites
C
C
Acidic/basic properties of amino acids

Amino acid structure and protein structure
Background
This study focuses on the characteristics of the abundant plasma protein referred to as histidineproline-rich glycoprotein (HPRG). HPRG is so named because it has a very high content of histidine (13
mol %). The human HPRG contains a pentapeptide GHHPH sequence repeated in tandem twelve times.
The authors of this study hypothesized that its high histidine content might allow HPRG to play a role in
regulating local pH in the blood. The local pH in blood may drop a half a pH unit during lactic acidosis or
even a full pH unit in hypoxia or ischemia. In the case presented here, the binding of HPRG to glycosaminoglycans was investigated. Glycosaminoglycans are anionic polysaccharides that are the major
component of the ground substance that forms the matrix of the extracellular spaces of the connective
tissue in blood vessel walls. In this study, the binding of HPRG to the glycosaminoglycan heparin was
measured. Based on their results, the investigators propose a model which describes how binding of
HPRG to glycosaminoglycans may allow HPRG to regulate local blood pH.
Questions
1. Binding studies were carried out in which
heparin was immobilized on the surface of
cuvettes. The pH dependence of HPRG
binding to heparin was investigated and the
results are shown in Figure 2.1.
a. How is the binding of HPRG to heparin
dependent on pH? Give structural
reasons for the binding dependence. The
structure of heparin is shown in Figure
2.2.
b. The same binding studies were carried
Figure 2.1: The pH-dependence of HPRG binding
out in which HPRG was reacted with
to unmodified heparin and heparin modified with
diethylpyrocarbonate (DEPC), a comDEPC (based on Borza and Morgan, 1998).
pound that specifically reacts with histidine residues. The reaction is shown in Figure 2.3. Explain the results.
CASE 2 C Histidine-Proline-rich Glycoprotein as a pH sensor
Figure 2.2: Repeating disaccharide unit of heparin.
Figure 2.3: Reaction of histidine side chains with diethylpyrocarbonate.

2

2. The effect of transition metals on the binding of HPRG was investigated next. The ability of
increasing concentrations of Cu2+ and Zn2+ to promote HPRG binding to heparin at pH = 7.3 was
measured. The results are shown in Figure 2.4. In addition, the binding of HPRG to heparin in the
presence of these ions was compared at various pHs. Figure 2.5 shows the comparison of binding at
pH = 6.0 and at pH = 7.4 in the presence of 5.2 nM zinc. What is your interpretation of these results?
Figure 2.4: Binding of HPRG to heparin in the presence

and of copper and zinc ions (based on Borza and Morgan,
1998).
Figure 2.5: Binding of HPRG to heparin in the presence and

absence of 2.5 nM zinc ions at two different pH values (based on
Borza and Morgan, 1998).

3. Local cellular pH can decrease from one-half to one pH unit depending on a variety of circumstances
including ischemia, hypoxia, and inflammation due to lactic acidosis. In addition, metabolic acidosis is
often one of the symptoms in complications following surgery. The investigators have proposed that
HPRG acts to relieve the acidosis in these circumstances. Propose a model that explains the
mechanism of pH regulation by HPRG.
4. Other plasma proteins have been studied for their ability to bind to glycosaminoglycans. One such
protein is kininogen, which is a lysine-rich protein. Like HPRG, kininogen is able to bind to
glycosaminoglycans, but this binding is far less sensitive to small fluctuations in physiological pH.
a. Why does kininogen bind to glycosaminoglycans easily?
b. Why is the binding of kininogen less sensitive to physiological pH changes?
References
Borza, D-B., and Morgan, W. T. (1998) J. Biol. Chem., 273, pp. 5493-5499.
Lundblad, R. (1995) Techniques in Protein Modification, CRC Press, Boca Raton, FL, p. 111.

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Case 2

Histidine-Proline-rich Glycoprotein as a Plasma pH Sensor

Acidic/basic properties of amino acids

CASE 2 C Histidine-Proline-rich Glycoprotein as a pH sensor

Figure 2.2: Repeating disaccharide unit of heparin.

Figure 2.3: Reaction of histidine side chains with diethylpyrocarbonate.

CASE 2 C Histidine-Proline-rich Glycoprotein as a pH sensor

Figure 2.4: Binding of HPRG to heparin in the presence

Figure 2.5: Binding of HPRG to heparin in the presence and

CASE 2 C Histidine-Proline-rich Glycoprotein as a pH sensor

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