Lab Genetic 8

RESULT:
A : DNA : The Genetic Code

Base Pairing
Select one of the remaining nucleotides and attempt to pair it with a nucleotide of the strand by
putting the ends of their bases together. Attempt several such pairings using different bases. Then
fill in the following :
Adenine pairs only with thymine.
Cytosine pairs only with guanine .
Bases which pair are said to be complementary. The bonds between the bases are not covalent
bonds, but much weaker hydrogen bonds.
DNA Structure
Duplicate Figure 2 by pairing each nucleotide of the first strand with its complementary
nucleotide and joining the bonds to form a new strand. Notice that the two strands are
antiparallel (they run in opposite directions); that is, if one strands runs 5 1 to 31 (phosphate-sugar,
phosphate sugar) from top to bottom, the other must run 3 1 to 51 from top to bottom. The
structure which is produced represents the double-stranded DNA molecule. Using A, C, G, T
indicate on Figure 2 the base sequence which have produced for each side of the molecule.
The Genetic Code
The sequence of bases in DNA forms a code or set of instructions for proteins synthesis. Each
code word or codon consists of a sequence of three bases and specifies a particular amino acid.
There are 64 possible codon (Figure 3). Some are chain terminating codons which are not known
to specify any amino acid. These indicate where a protein chain is to end. In most cases, an
amino acid can be specified by more than one codon, but each codon can specify only one amino
acid. Usually one strand (The sense strand) od DNA is active in protein synthesis. The codons of
the inactive or antisense strand are not used in protein synthesis. Consider the right side of the
molecule that you have constructed to be the sense strand and read the codons from the 3 1 end of
the strand toward 51 end of the strand toward the 51 end
Which amino acids are specified by the two codons?
=Amino acids are specified by the two codons are Tyrosine (Tyr) and Glutamine (Gln).
B : RNA : The Code Transcribed

RNA is produced by using DNA as pattern. During this process, called transcription, the genetic
code is transferred from DNA to RNA.
Obtain the following units for group : 12 Deoxyribose (Red), 12 Ribose (Pink), 24 Phosphate, 4
Adenine (A), 8 Cytosine (C), 8 Guanine (G), 2 Thymine (T), 2 Uracil (U). During the exercise,
always keep the printed side of each piece up.
Nucleotides
Compare a deoxyribose unit to a ribose unit. Look at the formula of each molecule as printed on
the units.
How does deoxyribose differ from ribose sugar?
= The deoxyribosediffers from ribose sugar because deoxyribose is a component of DNA which
is lacks OH (alcohol group) on the 21 carbon ring. While ribose is a component of RNA which
contains OH group on the 211 carbon ring. The main difference between the two is that ribose
has an oxygen atom attached to carbon 2 .
The nucleotides of DNA contain deoxyribose sugar while nucleotides of RNA contain ribose
sugar.
Use the deoxyribose units to form the following DNA nucleotides:
2adenine, 6 cytosine, 2 guanine, and 2 thymine. Now use the ribose units to form the following
RNA nucletides: 2 adenine, 2 cytosine, 6 guanine, and 2 uracil.
Transcription
During transcription the two strands of a DNA molecule become separated along part of the
molecules length. One strand of DNA remains inactive, but the other, the sense strand, is used to
bring about the synthesis of RNA.
To demonstrate transcription, first use the deoxyribose nucleotides to form a single strand of
DNA. We will use this as the sense strand and ignore the antisense strand. Beginning at the 3 1
(sugar) end of the strand, pair each DNA nucleotide with its RNA complement (uracil of RNA
complements adenine of DNA) and link together the phosphate and ribose units to form a strand
of RNA synthesis begins at the end 5 1 end of the RNA strand and proceeds toward its 3 1 end).
Separate the RNA and DNA strands. Once RNA is formed, it is released from DNA and goes to
other parts of the cell.
How does transcription differ from replication?
=Transcription differs from replication because DNA replication occurs in preparation for cell
division, while transcription happens in preparation for protein translation. DNA replication is
important for properly regulating the growth and division of cells. The DNA will not replicate if
the cell lacks certain growth factors, thereby keeping the cell division rate under control.
Transcription of DNA is the method for regulating gene expression. Although all of our cells
contain copies of all of our genes, each cell only expresses, or turns on, the genes that are
necessary for the functions of that cell. Transcription only occurs when a gene is turned on.
Types of RNA
Transcription produces three major types of RNA : ribosomal (mRNA), transfer (tRNA), and
messenger (mRNA). Ribosomal RNA combines with proteins to form bodies called ribosomes.
The ribosomes become located in the cells cytoplasm and are centers for protein synthesis.
Transfer RNA reacts with amino acids in the cytoplasm and interacts with ribosomes and
messenger RNA in the assembly of amino acids into proteins. It is messenger RNA that brings
the instructions for protein synthesis (the genetic code) from DNA in the nucleus to the
ribosomes.
Messenger RNA and The Genetic Code

Look again at the sense strand of DNA and the RNA strand produced by it. Notice that the
sequence of bases in RNA is controlled by the sequence of bases in DNA; that is, the RNA
codons (triplets of bases) are complementary to the DNA codons. Figure 1 gives the possible
DNA codons with their corresponding mRNA codons and the amino acids specified by each. (in
the table, the mRNA codons are read from the 51 end of the molecule to the 31 end).
Which amino acids are specified by the RNA strand that you have assembled?
= From 3 to 5, the amino acids that had been obtained are Histidine (CAC) and Leucine (CUG)

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RESULT:

A : DNA : The Genetic Code

B : RNA : The Code Transcribed

Messenger RNA and The Genetic Code

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