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Vitamin C Determination by Iodine Titration

Vitamin C content in foods can be determined through iodine titration. Iodine solution is used as the titrant and reacts with vitamin C, converting iodine to iodide ion. Once all the vitamin C is oxidized, iodine will react with starch to form a blue-black complex, indicating the endpoint of the titration. The procedure involves weighing fruit, adding sulfuric acid and starch solution, then titrating with iodine solution. The amount of iodine consumed can then be used to calculate the amount of vitamin C.

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199 views1 page

Vitamin C Determination by Iodine Titration

Vitamin C content in foods can be determined through iodine titration. Iodine solution is used as the titrant and reacts with vitamin C, converting iodine to iodide ion. Once all the vitamin C is oxidized, iodine will react with starch to form a blue-black complex, indicating the endpoint of the titration. The procedure involves weighing fruit, adding sulfuric acid and starch solution, then titrating with iodine solution. The amount of iodine consumed can then be used to calculate the amount of vitamin C.

Uploaded by

Abdul Majid Ia
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Vitamin C Determination by Iodine Titration

One way to determine the amount of vitamin C in food is to use a redox titration. In this titrartion
the titrant will be I2 solution. Iodine is relatively insoluble, but this can be improved by
complexing the iodine with iodide-ion (I2 +KI solution)

As long as vitamin C is present in the solution, the iodine is converted to the iodide ion very
quickly. Howevever, when the all the vitamin C is oxidized, iodine will be present, which react
with starch to form a blue-black complex. The blue-black color is the endpoint of the titration.

Description of the procedure:

Weigh about 10 grams of fruit, slice it , and then triturated in a mortar. Measure 10 cm3 of
sulfuric acid with a cylinder and add it to the fruit suspension. Then wash all the suspension into
an Erlenmeyer flask then add 1 cm3 starch solution. Titrate it with 0.0025 M iodine solution.
From the consumed amount of iodine the vitamin C content of the fruit can be determined.

The process of calculation:


o Calculate how much iodine consumed (in moles) in the solution!
o Write the reaction equation and than conclude how much vitamin C (in moles) was in the
Erlenmeyer flask!
o Calculate the amount of vitamin C in grams!
o Calculate how many grams of vitamin C are in 100 gram fruit!

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