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    Chol

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    Enrique Duarte
    This document provides instructions for validating a cholesterol assay on an ARCHITECT C8000 instrument. It outlines the instrument settings, reaction definition, reagent and sample details, calibration information including standard levels and intervals, and validity check parameters for the CHOL reagent which uses a cholesterol oxidase/peroxidase enzymatic method. Results are reported in mg/dL with 1 decimal place.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Chol

    Uploaded by

    Enrique Duarte
    37 views1 page
    This document provides instructions for validating a cholesterol assay on an ARCHITECT C8000 instrument. It outlines the instrument settings, reaction definition, reagent and sample details, calibration information including standard levels and intervals, and validity check parameters for the CHOL reagent which uses a cholesterol oxidase/peroxidase enzymatic method. Results are reported in mg/dL with 1 decimal place.

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    This document provides instructions for validating a cholesterol assay on an ARCHITECT C8000 instrument. It outlines the instrument settings, reaction definition, reagent and sample details, calibration information including standard levels and intervals, and validity check parameters for the CHOL reagent which uses a cholesterol oxidase/peroxidase enzymatic method. Results are reported in mg/dL with 1 decimal place.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    37 views1 page

    Chol

    Uploaded by

    Enrique Duarte
    This document provides instructions for validating a cholesterol assay on an ARCHITECT C8000 instrument. It outlines the instrument settings, reaction definition, reagent and sample details, calibration information including standard levels and intervals, and validity check parameters for the CHOL reagent which uses a cholesterol oxidase/peroxidase enzymatic method. Results are reported in mg/dL with 1 decimal place.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    CHOLESTEROL

    Enzymatic-spectrophotometric
    CHOLESTEROL OXIDASE/PEROXIDASE

    The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
    instrument. It is advisable to validate the use when there is any change in software or reagent versions.

    Instrument: ARCHITECT C8000


    Reagent preparation
    Reagent is ready to use.

    Instrument settings

    General Conventional Units


    Assay CHOL Type Photometric Results Unit mg/dL
    Number ... Version 1 Assay Defaults Low Linearity 0.3
    High Linearity 1000
    Reaction Definition
    Reaction Mode End Up Read Times Gender and Age specific Ranges
    Wavelength 500 / 0 Main 16 -- 18 GENDER AGE (Y) NORMAL
    Last Required Read 18 Flex __ -- __ Male 0-130 0 - 239
    Absorbance Range … / … Color Correction __ -- __ Female 0-130 0 - 239
    Sample Blank Type None Blank Read Time __ -- __ Either 0-130 0 - 239

    Reagent / Sample R1 R2 Reults Units mg/dL


    Reagent CHOL Reagent Volume 300 Decimal Places 1 (Range 0-4)
    Diluent Saline Water Volume __ __ Correlation Factor 1.0000
    Diluent Disp. Mode Type 0 Dispense Mode Type 0 Type 0 Intercept 0.0000

    Diluent Name Sample Water Dilution Factor


    Standard 3 1:1.00

    Validity Checks
    Reaction definition None
    Rate Linearity % ___
    Calibration
    Method Linear

    Calibrators and Volumes


    Calibrator Set Standard Calibrator Level Concentration Volume
    Blank 0.0 3
    Std. 1 (*) 3
    Std. 2 -- --
    Std. 3 -- --
    Std. 4 -- --
    Replicates 3 (Range 1-3) Std. 5 -- --

    Intervals Validity Checks


    Calibration Interval Full Interval 999 hours Blank Absorbance range: 0.0000 -- 0.0000
    Calibration Type Adjust Type None Span Absorbance Range: 0.0000 -- 0.0000
    Expected cal Factor : 0.00
    Expected Cal factor tolerance: %0
    (*) Enter the standard value
    … Values to be entered by the user
    Version 0707

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