Chapter Outline: ELL Tructure and Unction

Download as pdf or txt
Download as pdf or txt
You are on page 1of 5

CHAPTER 4 CELL STRUCTURE AND FUNCTION

Chapter Outline
4.1 Cellular Level of Organization
A. Cell Theory
1. In 1830s, Matthias Schleiden studying plants and Theodor Schwann studying animals independently
declared these organisms were made of cells.
2. All organisms, both unicellular or multicellular, are made up of cells.
3. Cells are the smallest units of living matter and are the structural and functional units of all organisms.
4. Cells are capable of self-reproduction; Rudolf Virchow declared cells come only from preexisting
cells.
B. Cell Size
1. Cells range in size from a frog’s egg (one millimeter) down to one micrometer.
2. Cells need a surface area of plasma membrane large enough to adequately exchange materials.
3. Surface-area-to-volume ratio requires that cells be small.
a. As cells get larger in volume, surface area relative to volume decreases.
b. Size limits how large the actively metabolizing cells can become.
c. Cells that need greater surface area use modifications such as folding, microvilli, etc.
C. Microscopy of Today
1. Bright-field microscope uses light rays focused by glass lenses.
2. Transmission electron microscope (TEM) uses electrons passing through specimen; focused by
magnets.
3. Scanning electron microscope (SEM) uses electrons scanned across metal-coated specimen.
4. Magnification is a function of wavelengths; the shorter wavelengths of electrons allow greater
magnification.
5. Resolution is the minimum distance between two objects before they are seen as one larger object.
6. Immunofluorescence microscopy uses fluorescent antibodies to reveal proteins in cells.
7. Confocal microscopy uses laser beam to focus on shallow plane; this forms a series of optical
sections.
8. Video-enhanced contrast microscopy accentuates the light and dark regions and may use a computer
to contrast regions with false colors.
9. Bright-field, phase contrast, differential interference and darkfield are different types of light
microscopy that improve our ability to see various features.
4.2 Prokaryotic Cells
A. Types of Prokaryotic Cells
1. All prokaryotic cells lack a nucleus and are smaller and simpler than eukaryotic cells.
2. Prokaryotic cells were the first cells and date back to earliest evolutionary history.
3. Because they are biochemically different, prokaryotes are divided into two domains: Bacteria and
Archaea.
B. The Structure of Bacteria
1. Most bacteria average 1–1.4 µm wide and 2–6 µm long , just visible with light microscopes.
2. Bacteria occur in three basic shapes: spherical coccus, rod-shaped bacillus, and spiral spirillum.
3. Cell Envelope
a. A plasma membrane is the same as eukaryotic cells: a phospholipid bilayer.
b. The plasma membrane can form internal pouches called mesosomes that increase surface area for
enzymes and metabolism.
c. A cell wall holds the shape of the cell ans is strengthened by peptidoglycan.
d. A glycocalyx is a layer of polysaccharides on the outside of the cell wall; it is called a capsule if
organized and not easily washed off.
4. Cytoplasm
a. The cytoplasm contains a semifluid solution with enzymes that carry on the chemical reactions
that maintain the bacterium.
b. The nucleoid is a region that contains the genes in a single, circular DNA molecule.
c. Plasmids are small accessory rings of DNA aside from the nucleoid.
d. Ribosomes are particles with two subunits that synthesize proteins.
e. Inclusion bodies are stored granules of substances in the cytoplasm..
f. Cyanobacteria are bacteria that photosynthesize; they lack chloroplasts but have thylakoid
membranes with chlorophyll and other pigments.
5. Appendages
a. Motile bacteria usually have flagella; the filament, hook and basal body work to rotate the
flagellum like a propeller to move through fluid medium.
b. Fimbriae are short appendages that help them attach to an appropriate surface.
c. Sex pili are tubes used by bacteria to pass DNA from cell to cell.
C. The Structure of Archaea
1. In addition to spheres, rods, and spirals, Archaea can also be lobed, plate-shaped, and just irregular.
2. The cell was has various polysaccharides rather than peptidoglycan.
3. The DNA and RNA base sequences are closer to eukaryotes than bacteria.
4. Many Archaea are found in extremely salty or hot environments and may have been the first cells.
4.3 Eukaryotic Cells
A. Eukaryotic Cells
1. Eukaryotic cells are members of the domain Eukarya, including kingdoms Fungi, Animalia, Plantae,
and Protista.
2. A membrane-bounded nucleus houses DNA.
3. The nucleus may have originated as an invagination of the plasma membrane.
B. The Cytoplasm and the Organelles
1. Eukaryotic cells have a cytoplasm that includes everything outside the nucleus and inside the plasma
membrane.
2. The cytoplasm is compartmentalized with small organelles that perform special functions.
3. The mitochondria and chloroplasts are unique in being self-sufficient and having their own genetic
material.
4. Other organelles communicate with each other and exchange products in transport vesicles.
C. The Cytoskeleton
1. The cytoskeleton is a lattice of protein fibers that maintains the shape of the cell.
2. Protein fibers serve as tracts of transport for vesicles that travel between organelles.
D. The Outer Boundary
1. The plasma membrane separates cell contents from the environment and regulates passage into and out
of the cell.
2. The plasma membrane is a phospholipid bilayer (this will be detailed in Chapter 5).
3. Proteins within the membrane can be receptors that bind to vesicles or molecules.
4. In plant cells, the outer boundary includes a cell wall with cellulose fibers (in contrast to the bacterial
cell wall).
5. A cell wall does not interfere with the function of the cell membrane.
E. The Nucleus
1. Structures
a. The nucleus has a diameter of about 5 µm.
b. Chromatin is a threadlike material that coils into chromosomes just before cell division occurs;
contains DNA, protein, and some RNA.
c. Nucleoplasm is the semifluid medium of nucleus.
d. Chromosomes are rodlike structures formed during cell division; it is coiled or folded chromatin.
e. The nucleolus is a dark-staining spherical body inside the nucleus; it is the site where rRNA joins
proteins to form ribosomes.
f. The nucleus is the site of DNA and determines characteristics of the cell by coding for proteins.
g. The nuclear envelope is a double membrane separating nucleoplasm from cytoplasm.
h. Nuclear pores (100 nm) in the nuclear membrane permit passage of proteins into the nucleus and
mRNA and ribosomal subunits out of the nucleus.
F. Ribosomes
1. Ribosomes of eukaryotic cells are 20 nm by 30 nm; ribosomes in prokaryotic cells are slightly smaller.
2. Ribosomes are composed of a large and a small subunit.
3. Each subunit has its own mix of proteins and rRNA.
4. In eukaryotic cells, polyribosomes are several ribosomes synthesizing same protein; they may be
attached to ER or may float free.
5. Ribosomes attached to ER depend on an ER signal sequence to bind to a receptor protein.
6. Ribosomes coordinate the assembly of amino acids into polypeptide chains; this protein synthesis
results in new proteins within the interior of the ER.
E. The Endomembrane System
1. Endomembrane system is a series of intracellular membranes that compartmentalize the cell.
2. This system compartmentalizes a cell into particular regions; vesicles transport molecules in the cell.
3. Endoplasmic Reticulum
a. Endoplasmic reticulum (ER) is system of membrane channels continuous with outer membrane of
the nuclear envelope.
b. Rough ER is studded with ribosomes on the cytoplasm side; it is the site where proteins are
synthesized and enter the ER interior for processing and modification.
c. Smooth ER is continuous with rough ER but lacks ribosomes; it is a site of various synthetic
processes, detoxification, and storage; smooth ER forms transport vesicles.
4. The Golgi Apparatus
a. The Golgi apparatus is named for Camillo Golgi, who discovered it in 1898.
b. The Golgi apparatus consists of a stack of 3–20 slightly curved saccules.
c. The Golgi apparatus receives protein-filled vesicles that bud from the ER.
d. Vesicle fuses with the membrane of a Golgi apparatus or moves to the outer face after proteins are
repackaged.
e. Vesicles formed from the membrane of outer face of the Golgi apparatus then move to different
locations in a cell; at the plasma membrane they discharge their contents as secretions, a process
called exocytosis because substances exit the cytoplasm.
5. Lysosomes
a. Lysosomes are membrane-bounded vesicles produced by the Golgi apparatus and contain
digestive enzymes.
b. Lysosomes contain powerful digestive enzymes that are highly acidic.
c. Macromolecules enter a cell by vesicle formation; lysosomes fuse with vesicles and digest the
contents of the vesicle.
d. White blood cells that engulf bacteria use lysosomes to digest bacteria.
e. Autodigestion occurs when lysosomes digest parts of cells.
f. Apoptosis is programmed cell death, a normal part of development (e.g., tadpole tail absorption,
degeneration of webbing between human fingers).
6. Endomembrane System Summary
a. Proteins produced in rough ER and lipids from smooth ER are carried in vesicles to the Golgi
apparatus.
b. The Golgi apparatus modifies these products and then sorts and packages them into vesicles that
go to various cell destinations.
c. Secretory vesicles carry products to the membrane where exocytosis produces secretions.
d. Lysosomes fuse with incoming vesicles and digest macromolecules.
F. Peroxisomes
a. Peroxisomes are membrane-bounded vesicles that contain specific enzymes.
b. Peroxisome action always results in production of hydrogen peroxide.
c. Hydrogen peroxide (H2O2) is broken down to water and oxygen by catalase.
d. Peroxisomes in the liver produce bile salts from cholesterol and also break down fats.
e. Missing or inactive lysosomal enzymes cause serious childhood diseases; the boy in the movie
Lorenzo’s Oil is an example.
f. Peroxisomes also occur in germinating seeds where they convert oils into sugars used as nutrients by
growing plant and in leaves where they give off CO2 that can be used in photosynthesis.
G. Vacuoles
a. Vacuoles in some protists are specialized and include water-regulating contractile vacuoles.
b. In plants, a large prominent vacuole is water-filled and gives support to cell.
c. The central plant stores water, sugars, salts, pigments, and toxic substances to protect plant from
herbivores.
d. A central vacuole eventually stores plant wastes since no system for excreting wastes occurs in plants.
H. Energy-Related Organelles
1. Chloroplasts and mitochondria are membranous organelles that serve as sites of photosynthesis and
cellular respiration, respectively.
a. Photosynthesis is represented by the equation:
solar energy + carbon dioxide + water → carbohydrate + oxygen
b. Cellular respiration is represented by the equation:
carbohydrate + oxygen → energy + carbon dioxide + water + energy (in ATP)
c. In eukaryotes, photosynthesis occurs in chloroplasts and cellular respiration occurs in
mitochondria.
d. Only plants, algae, and certain bacteria are capable of carrying on photosynthesis.
2. Chloroplasts
a. Chloroplasts are a type of plastid; plastids in a plant species all contain copies of the same DNA.
b. Chloroplasts are twice as wide and five times as long as mitochondria.
c. Chloroplast is bounded by a double membrane organized into flattened disc-like sacs called
thylakoids formed from a third membrane.
d. Chlorophyll and other pigments that capture energy are located within the thylakoid membrane.
e. Chloroplasts have both their own DNA and ribosomes, supporting the endosymbiotic hypothesis.
3. Mitochondria
a. Mitochondria vary and may be longer and thinner or short and broad.
b. Mitochondria also can be fixed in one location or form long, moving chains.
c. Mitochondria have a double membrane; the inner membrane has folds (cristae) that project into
the matrix.
d. Mitochondria contain ribosomes and their own DNA, again supporting the endosymbiotic
hypothesis.
e. The matrix of the mitochondria is concentrated with enzymes that break down carbohydrates.
f. ATP production occurs on the inner membrane (cristae).
G. The Cytoskeleton
1. Cytoskeleton is a network of connected filaments and tubules; it extends from the nucleus to the
plasma membrane in eukaryotes.
a. Electron microscopy reveals an organized cytosol.
b. Immunofluorescence microscopy identifies protein fibers.
c. Elements of the cytoskeleton include: actin filaments, intermediate filaments, and microtubules.
2. Actin Filaments
a. Actin filaments are long, thin fibers (about 7 nm in diameter) that occur in bundles or meshlike
networks.
b. The actin filament consists of two chains of globular actin monomers twisted to form a helix.
c. Actin filaments play a structural role, forming a dense complex web just under the plasma
membrane; this accounts for the formation of pseudopods in amoeboid movement.
d. Actin filaments in microvilli of intestinal cells likely shorten or extend cell into intestine.
e. In plant cells, they form tracks along which chloroplasts circulate.
f. Actin filaments move by interacting with myosin: myosin combines with and splits ATP, binding
to actin and changing configuration to pull actin filament forward.
h. Similar action accounts for pinching off cells during cell division.
3. Intermediate Filaments
a. Intermediate filaments are 8–11 nm in diameter, between actin filaments and microtubules in size.
b. They are rope-like assemblies of fibrous polypeptides.
c. Some support the nuclear envelope; others support plasma membrane and form cell-to-cell
junctions.
4. Microtubules
a. Microtubules are small hollow cylinders (25 nm in diameter and from 0.2–25 µm in length).
b. Microtubules are composed of a globular protein tubulin that occurs as α tubulin and β tubulin.
c. Assembly brings these two together as dimers and the dimers arrange themselves in rows.
d. Regulation of microtubule assembly is under control of a microtubule organizing center (MTOC):
a centrosome.
e. Microtubules radiate from the MTOC, helping maintain shape of cells and acting as tracks along
which organelles move.
f. Similar to actin-myosin, the motor molecules kinesin and dynein are associated with microtubules.
g. Different kinds of kinesin proteins specialize to move one kind of vesicle or cell organelle.
h. Cytoplasmic dynein is similar to the molecule dynein found in flagella.
5. Cytoskeleton Overview
a. The cytoskeleton is the cellular analogy to the bones and muscles of an animal.
b. The cytoskeletal elements can rapidly assemble and disassemble, as during cell division.
c. Temporary formation of a spindle distributes chromosomes in an orderly manner.
6. Centrioles
a. Centrioles are short cylinders with a ring pattern (9 + 0) of microtubule triplets.
b. In animal cells and most protists, centrosome contains two centrioles lying at right angles to each
other.
c. Plant and fungal cells have equivalent of a centrosome but it does not contain centrioles.
d. Centrioles serve as basal bodies for cilia and flagella.
7. Cilia and Flagella
a. Cilia are short, usually numerous hairlike projections that can move in an undulating fashion (e.g.,
Paramecium; lining of human upper respiratory tract).
b. Flagella are longer, usually fewer, whip-like projections that move in whip-like fashion (e.g.,
sperm cells).
c. Both have similar construction, but differ from prokaryotic flagella.
1) Membrane-bounded cylinders enclose a matrix containing a cylinder of nine pairs of
microtubules encircling two single microtubules (9 + 2 pattern of microtubules).
2) Cilia and flagella move when the microtubules slide past one another.
3) Cilia and flagella have a basal body at base with same arrangement of microtubule triples as
centrioles.
4) Cilia and flagella grow by the addition of tubulin dimers to their tips.

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy