BIOSTAT ® D-DCU

Your “Fast Lane” to Production

BIOSTAT ® D-DCU
Your “Fast Lane” to Production

The BIOSTAT ® D-DCU is a compact bioprocess system available in microbial

or cell culture versions with vessel choices from 10 to 200L working volume.

The optimized and proven design of the BIOSTAT® D-DCU is the result of
thorough analysis of the most required features and functions from over
thirty years of stainless steel fermenter | bioreactor design experience.
This standardized solution eliminates design times, allows faster delivery,
reduces cost, guarantees trouble free operation, allows for global service
support as well as spare part availability.

The BIOSTAT® D-DCU incorporates many The jacketed stainless steel culture vessel,
desirable and advanced features to with spiral baffles for efficient and
fulfill virtually any demand for modern homogenous heat transfer, are available in
bioprocess application, such as: Automatic 3:1 or 2:1 aspect height to diameter ratio.
Sterilization in Place (SIP), Cleaning in The gear free bottom drive agitation system
Place (CIP), dual pH and DO measurement provides long-term operation at minimal
­capability, lid lifting device, Water Intru- noise. The zero dead volume sanitary radial
sion Test (WIT)-Ready filter housings, dual diaphragm harvest and radial type sampling
exhaust filter housing line, tube and shell valves provide fresh samples without
exhaust cooler, exhaust heater, automatic residual pockets and are easy to clean and
or manual addition arrays as well as other maintain.
accessories. Furthermore, the ­BIOSTAT®
D-DCU is designed to interface single-use The supply unit includes all process piping
storage bags for media addition and har- for temperature control as well as the
vest as well as the TAKEONE® aseptic sam- exhaust and gas inlet lines. Due to the open
pling system. The modular approach allows frame design direct access for operation
multiple configurations (from baseline and easy maintenance is ensured. Further-
to fully featured) to meet every need and more, minimal floor contact points allow
budget. easy cleaning even underneath the skid.

Three subsystems comprise every An extended documentation and

BIOSTAT® D-DCU: qualification package is available to
– Control tower with integrated gas mixing support regulatory requirements.
and pump module
– Culture vessel with bottom agitation The BIOSTAT® D-DCU is available in both
system Single and Twin controller configuration.
– S upply Unit, open frame skid and compact It increases flexibility and allows control
stainless steel piping module of two separate processes at the same
time – even with different size culture
The control tower features best-in class vessels – but independently from each
control capabilities utilizing proven other.
industrial hardware. It is operated via a
simple and intuitive 19” touch screen
which keeps staff training to a minimum.
The compact design of the stainless steel
housing reduces the footprint and saves
precious space.
Features
– Single or Twin Configurations

–A
 vailable in incremental sizes from
10 to 200 L

– Preconfigured systems or choose from

an extensive list of options

–P
 owerful industrial rated DCU control
system with 19” TFT color touch screen

–A
 utomatic Sterilization in Place (SIP)
included

– Automatic Cleaning in Place (CIP)

optional

 esigned to interface single-use bags

–D
and sampling systems including the new
TAKEONE® aseptic sampling system

– Measurement and control opportunities

of pH, DO, temperature, foam, level,
vessel pressure, vessel weight, substrate
addition, gas mixing, agitation, gravi­
metric feed and harvest control, constant
total gas flow control, redox and
turbidity, weight of storage vessels etc.

– Up to six integrated peristaltic pumps per

vessel with options for fixed or variable
speed control

– Choice of polarographic or optical

DO sensors

– Superior gas mixing with up to six ­

flow meters and mass flow controllers

– Extended documentation package

available, including logbook and 3-Level
password protection

– Minimal floor contact points for ease

of cleaning

– Global spare part and service availability

Configure a System Utilizing Options
(Like Gasmix, CIP) from a Baseline Unit

Supply Unit
The Supply Unit includes all process piping for temperature
control as well as the exhaust and gas inlet lines. The open piping Supply Unit
frame and ergonomic design of the skid allows for good and
direct access to valves filter housings etc. All sanitary piping
is sloped | self drained. The Supply Unit for culture vessels with
10 – 30 L offers a choice of lockable casters or leveling | support
feet. The Supply Unit for culture vessel with 50 – 200 L is
equipped with leveling | support feet. Furthermore, the Supply
Units can be separated in two pieces allowing easy movement
to the site of installation.

Culture vessel
Available culture vessels from 10 L, 20 L, 30 L, 50 L, 100 L to
200 L working volume, with a total volume aspect ratio of (H:D)
2:1 or 3:1.

Sterilization in Place (SIP)

For ease of operation, automatic sterilization of the culture
vessel, gas inlet and exhaust gas flow path are included. Addition
groups, sampling valves and drain valves are either manually or
automatically sterilized.

NEW Cleaning in Place (CIP)

The BIOSTAT® D-DCU offers state of the art CIP solutions with inte-
grated SIP | CIP headers and now also with an optional mobile CIP
cart or the ability to connect to 3rd party CIP systems providing
an electronic handshake between the control systems. Integrated
CIP features allow the operator to effectively, reproducibly and
automatically clean the complete system including the culture Culture Vessel
vessel, gas inlets, exhaust lines, addition lines and transfer groups.

NEW Single-use sampling

The TAKEONE® aseptic sampling system
is single-use and delivered ready to use.
While traditional sampling devices require
cleaning, preparation and sterilization
after each use, the TAKEONE® single-use
sampling system saves valuable time by
being fully disposable.

Agitation
Bottom drive agitation is available with a double mechanical seal.
The high performance servo drive motor assembly combines low
shear, gentle agitation for cell cultures and high speed mixing for
microbial high cell density cultivation, ensuring high oxygen Agitation
transfer rates. The motor is gear-free for quiet operation – even
at high speed ranges.
 Control Tower
The Control Tower is available in single or twin configurations.
The integrated DCU control system belongs to the most proven
and advanced bioprocess controllers ever developed. Utilizing
proven technology and expert engineering, our existing in-house
systems bring powerful control capabilities to the sophisticated
biotechnology market. Proven industrial control hardware ensures
reliable system performance.

The DCU can be easily expanded and reconfigured to meet

evolving research or process requirements, including scale-up
from laboratory fermenters or scale-down to mimic production
process conditions.

For data logging, the Digital Control Unit (DCU) includes

a PC interface for SCADA software connection (for example
BioPAT® MFCS). DCU OPC communication software is available
for interface to other OPC compliant SCADA and DCS packages.
Using a local controller for local process control in combination
with a high level SCADA system ensures process control safety.
Control Tower
Intuitive Touchscreen
The control system presents an “intuitive-to-use”, large 19“ TFT
color touch screen for excellent local operation and process
control for each culture vessel. Clearly designed screens provide
an excellent process value overview and operation.

Intuitive Gassing Systems

Touchscreen A variety of spargers are available for microbial and cell culture
use. All systems provide individual flow rates and gas blending for
each culture vessel. Gas flow rates are adjustable via precision
flow meters with optional thermal mass flow controllers available
for each flow path (each gas).

Dosing Pumps
Up to six integrated fast load peristaltic pumps per vessel may
be chosen for addition of corrective agents, feeding, as well as
culture volume control. Up to four of the six can be analog speed
controlled pumps. Several ranges are available for both fixed and
speed controlled pumps. Additionally, external pumps for feeding
can be easily connected.
Gassing Systems

Dosing Pumps
BIOSTAT ® D-DCU
Gassing Strategies

Airflow
Utilizing one flow path for air, a flow meter visually indicates
and controls the sparger flow rate. An optional mass flow
controller may be integrated to control and measure the flow
range via manual adjustment or automatically in conjunction
with the DO controller.

Air Sparger

O2-Enrichment
Utilizing two flow paths for Air and O2 flows, the flow meters
visually indicate and allow manual adjustments of the sparger
flow rate. O2 is pulsed via solenoid valve, flowing only when
required to maintain the dissolved oxygen (DO) setpoint.
Air is not provided at this time. A mass flow controller can be
integrated to measure and control the total gas flow range via
manual adjustment or automatically in conjunction with the
DO controller.

O2-Enrichment, design up to 50 L/min

Air Sparger

O2

O2-Enrichment, design up to 300 L/min

Air Sparger

Flow meter
O2 Optional Gas switch
Dosing shut-off valve
Optional dosing shut-off valve
3-way dosing valve
Optional Mass Flow Controller
Mass Flow Controller
 Gas Flow Ratio
Utilizes two flow paths with mass flow control valves for Air
and O2 flow. Flow meters visually indicate the flow of Air and O2.
Both mass flow controllers can be operated manually or automat-
ically in conjunction with the DO controller.

Sparger
Air

O2

Advanced Additive Flow

Allows up to six gas flow paths. Solenoid valves select air, O2, N2
and CO2 for simultaneous flow to the sparger and air to overlay.
Up to six flow meters visually indicate and set the flow rate for
each gas. One additional gas flow path can be added to sparger
or overlay outlet. Furthermore, two 3-way solenoid valves can
be installed to switch the dedicated gas from sparger to overlay
(incl. soft switch). The design does support the installation of up
to six mass flow controllers, which makes constant sparger gas
flow c­ ontrol as well as constant overlay gas flow control possible.

Overlay

Air Sparger

O2

Flow meter
Optional Gas switch N2
Dosing shut-off valve
Optional dosing shut-off valve
3-way dosing valve CO2
Optional Mass Flow Controller
Mass Flow Controller
BioPAT ® DCU – Automation Solutions
for Advanced Process Control and Documentation

Our DCU (digital control unit) controller belongs to the most proven, ­reliable
and advanced bioprocess controllers ever developed. Use of a modular system
design has enabled us to offer a broad range of flexible and cost-effective
solutions for reusable and single-use systems from R&D to production. DCU
control systems are specially tailored for ­fermentation, cell culture and down
stream processing like cross flow ­filtration applications.

DCU control systems allow for independent and simultaneous operation of

multiple processes.

Discover the potential of our

standard software…
– Superior process value overview
– Sensor calibration
– In-process recalibration … and profit from advanced features
– Alarm monitoring – Overview of all vessels or single
– Trend display vessel display
– Automatic Sterilization in Place – Controller status indication
– Single or group calibration
– Advanced DO controller
– Gravimetric flow control for very precise
feeding
– Gravimetric harvest control
– Constant total gas flow control
– Automatic Cleaning in Place

SYNCHRONIZED

PAT
SOLUTIONS
BioPAT ® MFCS –
The Bioprocess SCADA System

BioPAT® MFCS is our SCADA software for supervisory bioprocess control and The optional, advanced version of
data acquisition. Provides GMP compliant documentation of your valuable BioPAT® MFCS includes modules such as:
– Multi-user network access for up to
process data and ensures reliable process control in combination with the 16 process units
advanced BIOSTAT® D-DCU for local process control. Supplied with every – Automation with recipes according
BIOSTAT ® D-DCU package, the new BioPAT® MFCS is ideal for efficient data to ANSI | ISA 88.01
acquisition and trend monitoring. – 21 CFR, Part 11 compatibility
– Multivariate Data Analysis modules

Learn more about the new BioPAT® MFCS:

www.sartorius-stedim.com/biopatmfcs

BioPAT ® SIMCA-Online
for Continuous Real-time Quality Control

Continuous real-time quality control and assurance is highly desired in

biopharmaceutical manufacturing. Unique on the market and developed
according to GAMP 5, BioPAT® SIMCA-online is your software solution
for real-time multi­variate statistical process monitoring and control.
The software permits early detection of process deviations. It provides user
guidance to simplify root-cause analysis by displaying easy-to-understand
graphics.
MVDA
Online Multivariate
Data Analysis
BIOSTAT ® D-DCU MO – Package Overview: O2-Enrichment

Package overview 10 L 20 L 30 L 50 L 100 L 200 L

RDD-M10L RDD-M20L RDD-M30L RDD-M50L RDD-M1HL RDD-M2HL
Cat. No. 208 VAC | Culture vessel H:D ratio
OBRES3 | 2:1 OBRES3 | 2:1 OBRES3 | 2:1 OBRES3 | 2:1 OBRES3 | 2:1 OBRES3 | 2:1
RDD-M10L RDD-M20L RDD-M30L RDD-M50L RDD-M1HL RDD-M2HL
Cat. No. 400 VAC | Culture vessel H:D ratio
OBRET4 | 3:1 OBRET4 | 3:1 OBRET4 | 3:1 OBRET4 | 3:1 OBRET4 | 3:1 OBRET4 | 3:1
Control Unit Twin configuration optional
Digital controller, color display with touch screen •
Control capabilities
Temperature, pH, DO (Multi stage cascade), Stirrer speed •
Substrate A and Substrate B •
Foam via conductive sensor •
High foam alarm •
Automatic full vessel sterilization sequence •
Gear and maintenance free agitation motor •
Gassing strategy O2-Enrichment (Airflow and Gas Flow Ratio optional)
Flow meter •
Solenoid valve for oxygen enrichment • (Mass Flow Controller optional)
3 for Acid | Base | Antifoam unused pump can be configured as substrate pump
Peristaltic pumps (integrated)
(Up to 6 pumps per site)
Supervisory Process Control Software
BioPAT® MFCS •
Supply frame Open frame design
Temperature control system Closed loop system with recirculation pump and heat exchanger for heating and cooling
Piping with valves and steam traps for automatic in-situ sterilization •
Culture Vessel Jacketed stainless steel vessel with vertical sight glass and bottom agitation
Stirrer shaft with Double Mechanical Seal (DMS) •
Condensate pressurization of buffer system DMS steam | compressed air • | O
6-blade disk impeller 3
Stainless steel filter housing for air Inlet and exhaust filter incl.
•
filter cartridges
Pressure gauge –1 | 3 barg •
Aeration tube with Ring sparger •
Exhaust cooler •
4-Baffles (removable) •
Resterilizable sampling valve •
1-Channel Sacova valve for needle free additions •
3-Channel Sacova valve for needle free additions •
Lamp for vessel illumination •
Storage bottles 3
Bottom harvest valve •
pH Electrode, cable •
DO Electrode, cable •
Pressure sensor, cable •
Foam sensor, cable •
Temperature sensor Pt 100 •
High-foam sensor with installation adaptor, cable •
Options
Mobile CIP unit with DCU interface O
Culture vessel weight measurement | control O | O
Automatic vessel pressure control O
Pressure hold test O
Lid lifting device 10-20 L | 30-200 L -- | O
Dual pH measurement | Dual DO measurement O | O
Containment sampling system O
WIT ready filter housings for Inlet and Exhaust filter O
Exhaust heater | Dual Exhaust filter line (parallel) O | O
Temperature measurement of condensate trap O
Cleaning in Place (CIP)  O
Resterilizable 4-valve addition array manual | automatic O | O
Automatic harvest valve O
Transfer group O
Speed controlled pumps for feeding O
Broad range of accessories available. Please contact us for further details. • = included, — — = unavailable, O = option
BIOSTAT ® D-DCU CC – Package Overview:
Advanced Additive Flow
Package overview 10 L 20 L 30 L 50 L 100 L 200 L
RDD-C10L RDD-C20L RDD-C30L RDD-C50L RDD-C1HL RDD-C2HL
Cat. No. 208 VAC | Culture vessel H:D ratio
ABRES3 | 2:1 ABRES3 | 2:1 ABRES3 | 2:1 ABRES3 | 2:1 ABRES3 | 2:1 ABRES3 | 2:1
RDD-C10L RDD-C20L RDD-C30L RDD-C50L RDD-C1HL RDD-C2HL
Cat. No. 400 VAC | Culture vessel H:D ratio
ABRES4 | 2:1 ABRES4 | 2:1 ABRES4 | 2:1 ABRES4 | 2:1 ABRES4 | 2:1 ABRES4 | 2:1
Control Unit Twin configuration optional
Digital controller, color display with touch screen •
Control capabilities
Temperature, pH, DO (Multi stage cascade), stirrer speed •
Substrate A – D •
Foam via conductive sensor •
High Foam alarm •
Automatic full and empty vessel sterilization sequence •
Gear and maintenance free agitation motor •
Gassing strategy Advanced Additive Flow
Flow meter sparger • for Air, O2 N2, CO2
Flow meter overlay • for Air
Automatic Gassing strategy of Air, O2, N2, CO2 for sparger • via solenoid valves (Mass Flow Controller optional)
Peristaltic pumps (integrated) 2 for Base | Afoam unused pump can be configured as substrate pump (Up to 6 pumps per system)
Data acquisition and trend monitoring software
BioPAT® MFCS •
Supply frame Open frame design
Temperature control system Closed loop system with recirculation pump and heat exchanger for heating and cooling
Solenoid valves and steam traps automatic in-situ sterilization •
Culture Vessel Jacketed stainless steel vessel with vertical sight glass and bottom agitation
Stirrer shaft with Double Mechanical Seal (DMS) •
Condensate pressurization of buffer system DMS steam | compressed air • | O
3-blade segment impeller 2
Stainless steel filter housing for Air Inlet (Sparger and Overlay)
•
and Exhaust filter incl. filter cartridges
Pressure gauge –1 | 3 barg •
Aeration tube with micro sparger •
Exhaust Cooler •
4-Baffles (removable) •
Resterilizable sampling valve •
1-Channel Sacova valve for needle free additions •
3-Channel Sacova valve for needle free additions •
Lamp for vessel illumination •
Storage bottles 2
Bottom harvest valve •
pH Electrode, cable •
DO Electrode, cable •
Pressure sensor, cable •
Foam sensor, cable •
Temperature sensor Pt 100 •
High-foam sensor with installation adaptor, cable •
Options
Mobile CIP unit with DCU interface O
Culture vessel weight measurement | control O | O
Automatic vessel pressure control O
Pressure hold test O
Lid lifting device 10-20 L | 30-200 L -- | O
Dual pH measurement | Dual DO measurement O | O
Containment sampling system O
WIT ready filter housings for Inlet and Exhaust filter O
Exhaust heater | Dual Exhaust filter line (parallel) O | O
Temperature measurement of condensate trap O
Cleaning in Place (CIP) O
Resterilizable 4-valve addition array manual | automatic O | O
Automatic harvest valve O
Transfer group O
Speed controlled pumps O
Broad range of accessories available. Please contact us for further details. • = included, — — = unavailable, O = option
BIOSTAT ® D-DCU – Technical Specification

Technical Specification 10 L 20 L 30 L 50 L 100 L 200 L

Space requirement Single [W + H + D] [“] 58.3 + 82.7 + 43.3 58.3 + 82.7 + 44.5 58.3 + 82.7 + 45.3 76.8 + 92.9 + 61.8 76.8 + 100.8 + 61.8 76.8 + 120.1 + 70.9
[m] 1.48 + 2.1 + 1.1 1.48 + 2.1 + 1.13 1.48 + 2.1 + 1.15 1.95 + 2.36 + 1.57 1.95 + 2.56 + 1.57 1.95 + 3.05 + 1.8
Space requirement Twin [W + H + D] [“] 84.6 + 82.7 + 43.3 84.6 + 82.7 + 44.5 84.6 + 82.7 + 45.3 122 + 92.9 + 61.8 122 + 100.8 + 61.8 122 + 120.1 + 70.9
[m] 2.15 + 2.1 + 1.1 2.15 + 2.1 + 1.13 2.15 + 2.1 + 1.15 3.1 + 2.36 + 1.57 3.1 + 2.56 + 1.57 3.1 + 3.05 + 1.8
Required wall opening dimensions [W + H] [“] 31.9 + 78.8 31.9 + 78.8 31.9 + 78.8 41.8 + 67 41.8 + 67 41.8 + 67
[m] 0.81 + 2 0.81 + 2 0.81 + 2 1.06 + 1.7 1.06 + 1.7 1.06 + 1.7
Culture vessel weight (approx.) [kg] 80 100 120 300 450 600
Supply Unit weight (approx.) [kg] 170 170 170 320 320 320
Control Tower weight (approx.) [kg]
160 | 205
Single | Twin
Ambient temperature | relative humidity
5 – 40°C | 85%
(non-condensating)
Utilities Requirements Conditions Max. Vessel Size
Flow 10 L 20 L 30 L 50 L 100 L 200 L
Process Air MO |  4 barg | 58 psig, controlled, [L/min] 15 |  30 |  45 |  75 |  150 |  300 | 
CC Sparger | Overlay Class 2 (ISO 8573-1) 1/10 2/20 3/30 5/50 10/100 20/200
O2 MO Sparger |  4 barg | 58 psig, controlled, prefiltered [L/min] 15 |  30 |  45 |  75 |  150 |  300 | 
CC Sparger | Overlay 1/5 2/10 3/15 5/25 10/50 20/100
CO2 MO Sparger |  4 barg | 58 psig, controlled, prefiltered [L/min] N | A N | A N | A N | A N | A N | A
CC Sparger | Overlay 1 | 5 2 | 10 3 | 15 5 | 25 10 | 50 20 | 100
N2 MO Sparger |  4 barg | 58 psig, controlled, prefiltered [L/min] N | A N | A N | A N | A N | A N | A
CC Sparger | Overlay 1 | 5 2 | 10 3 | 15 5 | 25 10 | 50 20 | 100
Utility steam 4 barg | 58 psig, controlled, prefiltered [kg/h] 15 15 15 50 90 160
Clean steam 1.5 barg | 21.8 psig, controlled, prefiltered [kg/h] 5 5 5 8 10 26
Cooling water 4 barg | 58 psig, controlled (15°C) prefiltered [L/min] 5 5 5 25 25 50
Cooling water return Atmospheric pressure to 1.5 barg | 21.8 psig [L/min] 5 5 5 25 35 50
CIP, cleaning and rinsing fluid 1.5 barg | 21.8 psig, controlled [L/min] on request 33 43 70
Condensate Atmospheric pressure (max. Temp. 98°C)
Instrument air 6 barg | 87 psig, controlled
Power supply (TNS net): 208 VAC/24A (FI switch intern 300mA) or 400 VAC/20A (FI switch intern 300mA)
5 wire: 3 + phase, 1 + ground, 1 + neutral
Power supply for electrical heater (TNS net): 208 VAC/16A or 400 VAC/10A
5 wire: 3 + phase, 1 + ground, 1 + neutral
Control Tower Integrated DCU-Controller, Gassing System and Pumps Single or Twin configuration
Controller Industrial PC (Siemens)
Housing material Stainless steel AISI 304
Display | Operation Touch Panel 19“ | Touch screen
Host communication Industrial Ethernet
External connections Expandable process I/O
Balance connection 3 per vessel; expandable of up to 6 per vessel
External Inputs | Off gas analyzer input 2 per vessel; Analog in (0 – 10 V) | 2 per vessel Analog in (4 – 20 mA)
External feed pumps up to 4 per vessel; 2 per vessel; Analog out (0 – 10 V)
Gassing System Up to 6 integrated Mass Flow Controllers and Flowmeter
MO application Air aeration, O2-Enrichment or Gas Flow Ratio; Max. total flow rate: 1.5 vvm
CC application Advanced Additive Flow; Max. total flow rate: Overlay 1 vvm | Sparger 0.1 vvm
Flow meter Air calibrated @ 4 barg 20°C | scale lenght 120 mmm
  Flow range 0.12–1.06 L/min up to 70–330 L/min
  Accuracy +/– 4% FS
Thermal Mass Flow Controller Air | N2, O2 or CO2 calibrated
  Flow range 0.02–1.0 sLpm up to 6–300 sLpm
  Accuracy +/– 1% FS
Integrated pumps Up to 6 per vessel (2 + digital + 2 + digital | speed controlled + 2 + speed controlled)
Pump head Watson Marlow 114 Watson Marlow 314
For tubings with 1.6 mm | 1/16“ wall thickness For tubings with bore 0.5-4.8 [mm] | 1/50-3/16[“] For tubings with bore 0.5-8.0 [mm] | 1/50-5/16[“]
Rotation speed [rpm] 5 44 up to 200 6 60 up to 200
Flow range [mL/min] Bore 0.5 mm 1/50“ 0.1 0.02 – 0.9 0.4 – 4 0.0 – 0.18 0.04 – 1.8 0.6 – 6
4.8 mm 3/16“ 0.09 – 4.3 0.75 – 37.4 17 – 170 2.3 – 11.4 2.3 – 114 38 – 380
8.0 mm 5/16“ N | A N | A N | A 0.48 – 24 4.8 – 240 80 – 800
Supply Unit Piping Skid in open frame design
Material | Surface finish (product wetted parts) Stainless steel AISI 316L | MO: Ra < 0.8 μm (31.5 Ra or better) | CC: Ra < 0.4 µm (15.7 Ra or better)
Temperature control system – steam version Closed loop thermostat system with recirculation pump, heat exchanger for cooling and heating or | and electrical heater
Temperature control (operation | sterilization): 8°C above cooling water to 90°C | up to 130°C
Heat exchanger (cooling | heating – steam version) Stainless steel, copper soldered, optional stainless steel | welded version available upon request
Electrical heater (optional) 6 kW (10–30 L: complete electrical heated; 50–200 L: auxiliary electrical heater only)
Culture vessel 10 L 20 L 30 L 50 L 100 L 200 L
H:D ratio 2:1 3:1 2:1 3:1 2:1 3:1 2:1 3:1 2:1 3:1 2:1 3:1
Total volume 14 L 15 L 29 L 31 L 42 L 41 L 74 L 77 L 152 L 152 L 313 L 323 L
Working volume 10 L 10 L 20 L 20 L 30 L 30 L 50 L 50 L 100 L 100 L 200 L 200 L
Minimal working volume 3.5 L 2.5 L 5.5 L 3.5 L 6.4 L 5.4 L 13 L 13L 24 L 24 L 47 L 41 L
Jacketed cylindrical part | Jacketed bottom yes | no yes | no yes | no yes | no yes | no yes | no yes | no yes | no yes | yes yes | no yes | yes yes | no
Weight lid with blind plugs [kg] 12 11 16 14 18 16 34 22 45 35 95 68
Agitation speed ranges for MO 20 –  20 –  20 –  20 –  20 –  20 –  20 –  20 –  20 –  20 –  20 –  20 – 
(max. impeller tip speed ≥ 5 m/s) 1500 1500 1200 1200 1100 1100 900 900 700 700 570 570
Motorpower | torque [kW | Nm] 2.3 | 5 2.3 | 5 3.1 | 9.4 3.1 | 9.4 3.1 | 9.4 3.1 | 9.4 4.2 | 16.2 4.2 | 16.2 4.9 | 26.7 4.9 | 26.7 6.6 | 48.2 6.6 | 48.2
Agitation speed ranges for CC
350 N | A 300 N | A 260 N | A 220 N | A 180 N | A 130 N | A
(max. impeller tip speed ≥ 2 m/s)
Motorpower | torque [kW | Nm] 2.3 | 5 N | A 2.3 | 5 N | A 2.3 | 5 N | A 3.1 | 9.4 N | A 4.2 | 16.2 N | A 4.2 | 16.2 N | A
Impeller to vessel diameter 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4
[Rushton impeller]
Impeller to vessel diameter 0.5 N | A 0.5 N | A 0.5 N | A 0.5 N | A 0.5 N | A 0.5 N | A
[3-blade segment impeller]
Lid ports 1 + sight glass for illumination 1 + sight glass for illumination
1 + port for Exhaust 1 + spare port DN 50
9 + 19 mm port 1 + port for CIP – connection
1 + port for Exhaust
8 + 19 mm port
3 + lifting eye
Upper side wall 4 + 25 mm port 3 + 25 mm port
1 + Sparger aeration 1 + Sparger aeration
1 + Overlay aeration | Bypass Sparger 1 + Overlay aeration | Spare
1 + port for rupture disc | safety valve 1 + port for rupture disc
1 + rectangular sight glass 1 + spare DN50
1 + rectangular sight glass
Lower side wall 5 + 25 mm port 5 + 25 mm port
1 + sanitary TC port 1 + sanitary TC port
1 + port for temperature sensor 1 + port for temperature sensor
Bottom 1 + flange for agitator 1 + flange for agitator
1 + Harvest | Drain valve 1 + Harvest | Drain valve
Jacket 1 + fluid in 1 + fluid in
1 + fluid out 1 + fluid out
Vessel design Jacketed stainless steel vessel with torospherical bottom and vertical sight glass Bottom agitation system
Material (product wetted parts) Stainless steel AISI 316 L | Borosilicat glass | EPDM (FDA aprroved)
Surface finish product wetted 2:1 Vessel Ra ≤ 0.4 µm (15.7 Ra or better), electropolished | 3:1 vessel: Ra ≤ 0.8 µm (31.5 Ra or better), electropolished
Pressure design criteria vessel | jacket –1/3 barg @ 150°C | –1/4 barg @ 150°C
Sensors | measurement ranges | resolution
Dissolved oxygen Polarographic or optical | 0–100% | 1% | 0.1%
pH Gel filled | 2–12 | 0.01 pH
Foam | Level | High Foam Conductive probe, stainless steel ceramic isolated
Temperature sensor Vessel/Jacket Pt100 | 0–150°C | 0.1 C / Pt100 | 0–150°C | 0.1 C
Redox Gel filled | –1000 – 1000 mV | 1 mV
Pressure Piezoresistive sensor | –0.5–2 [barg] | 1 mbar
Turbidity Single Channel NIR Absorption Probe, 0–6 AU | 0.01 AU
Regulatory compliance CE | UL | CSA (EN61010, UL61010); Culture vessel: ASME or PED or China pressure vessel regulation

MO: Microbial Application; CC: Cell Culture Application

Specifications are subject to change without notice
NEW Mobile CIP Unit – Technical Specification

Technical Specification
Design Mobile stainless steel skid
Dimensions (approx.) [W + H + D] [“] 72.9 + 64.6 + 30.7
[mm] 1851 + 1640 + 780
Weight (approx.) [kg] 100
Material | Surface finish (product wetted parts) Stainless steel AISI 316L | Ra < 0.8 μm (31.5 Ra or better) | EPDM | PVC | PE | PTFE | others
Utility Requirements Conditions | Flow Connection | Height
Water (WFI | PW | RO) min. 1 barg TC 64 | TC 2.5“ | 401 mm | 15.8“
Drain gravity | max. flow 3.5 m³ TC 64 | TC 2.5“ | 684 mm | 26.9“
Drain CIP pump gravity TC 25 | TC 1“ | 135 mm | 5.31“
Instrument air 6 barg | 87 psig, controlled Quick coupling
CIP supply line TC 64 | TC 2.5“
CIP return line TC 64 | TC 2.5“
Power supply (TNS net): 208 VAC | 60 Hz or 400 VAC | 50 Hz
5 wire: 3 + phase, 1 + ground, 1 + neutral
CIP pump
Type Centrifugal pump
Material pump head Stainless steel 316 L
Flow max. 3.5 m3/h @ 3 barg
Valves
Water Pneumatic operated diaphragm valve
Recirculation Pneumatic operated diaphragm valve
Drain Pneumatic operated diaphragm valve
Drain valve pump housing Manual operated diaphragm valve
Detergent dosing unit
Number of dosing units 1 (2nd optional)
Pump | Flow rate Diaphragm pump | up to 25 L/h
Storage container volume | Material 40 L | PE
Instrumentation | Measurement range
Function
Conductivity sensor (high) 1010 – 2000 mS
Conductivity sensor (low) 0.04 – 500 µS
Level switch Dry run protection for pump
Pressure gauge – 1/5 bar
External connections Connection to BIOSTAT® D-DCU for automated CIP sequence control (valves and recirculation pump)
Sales and Service Contacts
For further contacts, visit www.sartorius-stedim.com

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Kagyló u. 5 LLC “Biohit” Sartorius Stedim Biotech (Beijing) Co. Ltd.
2092 Budakeszi No. 33 Yu’an Road

Specifications subject to change without notice. Printed in the EU on paper bleached without chlorine. | W
Post Box 440 “B”
Phone +36.23.457.227 01001 Kiev, Ukraine Airport Industrial Park Zone B
Fax +36.23.457.147 Shunyi District, Beijing 101300
Phone +380.44.411.4918
Fax +380.50.623.3162 Phone +86.10.80426516
Italy Fax +86.10.80426580
Sartorius Stedim Italy S.p.A.
Via dell’Antella, 76/A Sartorius Stedim Biotech (Beijing) Co. Ltd.
50012 Antella-Bagno a Ripoli (FI) Shanghai Branch Office Publication No.: SBI1512-e150203 · Order No.: 85037-539-81 · Ver. 02 | 2015
Phone +39.055.63.40.41 3rd Floor, North Wing, Tower 1
Fax +39.055.63.40.526 No. 4560 Jin Ke Road
Pudong District, Shanghai 201210
Phone +86.21.68782300
Fax +86.21.68782332 | 68782882

Sartorius Stedim Biotech (Beijing) Co. Ltd.

Guangzhou Representative Office
Unit K, Building 23
Huihua Commerce & Trade Building
No. 80 Xianlie Middle Road
Guangzhou 510070
Phone +86.20.37618687 | 37618651
Fax +86.20.37619051

www.sartorius-stedim.com