Lab 6

EXPERIMENT 6
Dissolved Oxygen Levels in Natural Water
Group 1 CM011/B8
Sofia Isabelle G. Garcia
CE-1
REPORT
In this experiment the Laboratory questions and their answers are:
1. The water chemist waits until returning to the laboratory to fix the water sample for the
dissolved oxygen analysis. Will the reported dissolved oxygen concentration be reported
as too high, too low, or remain unchanged? Explain.
- When a water sample is left open in a room, the water could absorb carbon dioxide
CO2 from the atmosphere, thus further having greater amounts of oxygen in the water
sample. Due to this, the reported value of dissolved oxygen concentration will be too
high.
2. A solution of MnSO4 is added to fix the dissolved oxygen in the collected sample.
a. What is the meaning of the expression, “fix the dissolved oxygen,” and why is it so
important for the analysis of dissolved oxygen in a water sample?
- The meaning of the expression “fix the dissolves oxygen” is to transform the
dissolved oxygen in to a compound where it will not be away from the solution. o
b. Only an approximate volume (-1 mL) of MnSO4 is required for fixing the dissolved
oxygen in the sample. Explain why an exact volume is not critical.
- An exact volume of MnSO4 is not critical in the experiment because it is the excess
reactant while the dissolved oxygen is the limiting reactant.
3. No precipitate forms! Assuming the reagents were properly prepared and dispensed into
the sample, what might be predicted about its dissolved oxygen concentration? Explain.
- Since there were no precipitate formed, it can be said that the concentration of
dissolved oxygen in the sample is very little to the point that the value can be
disregarded already.
4. A water chemist measured and recorded the air temperature at 27°C when he should have
measured the water temperature. Which was only 21°C. As a result of this error, will the
dissolved oxygen concentration be reported as being higher or lower then it should be?
Explain.
- The reported concentration of dissolved oxygen will be higher than it should be.
Given that oxygen is less soluble in warm water than cold water, the concentrations
of dissolved oxygen decreases at the temperature of water increase.
5. The color of the analyte did not fade to form the light yellow-brown color but remained
intense even after the addition of a full buret of the S2O32- titrant, even though a
precipitate formed. What can be stated about the dissolved oxygen concentration of the
sample? Explain.
- MnO(OH)2 is more of brown in color and as well as brown precipitate but MnO2 can
also be brown.
6. Assuming a dissolved oxygen concentration of 7.0 ppm (mg/L) in a 200-mL water
sample,
a. How many moles of Mn (OH)3 will be produced with the addition of the MnSO4
solution?
7𝑚𝑔 𝑂2 1𝐿 1𝑔 1𝑚𝑜𝑙 𝑂2 4𝑚𝑜𝑙𝑀𝑛(𝑂𝐻)3
- 𝑚𝑜𝑙𝑀𝑛(𝑂𝐻)3 = 𝑥200𝑚𝐿 𝑥 𝑥 𝑥 𝑥
1𝐿 1000𝑚𝐿 1000𝑚𝑔 32𝑔 𝑂2 1𝑚𝑜𝑙 𝑂2
−4
- 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)3 = 1.75𝑥10 𝑚𝑜𝑙
b. How many moles of I3- will be produced when the KI-NaN3 solution is added to the
above solution?
1𝑚𝑜𝑙 𝐼3−
𝑚𝑜𝑙 𝐼3− = 1.75𝑥10−4 𝑚𝑜𝑙 𝑀𝑛(𝑂𝐻)3 𝑥
2𝑚𝑜𝑙𝑀𝑛(𝑂𝐻)3
- 𝑚𝑜𝑙 𝐼3− = 8.75𝑥10−5 𝑚𝑜𝑙
c. How many moles of S2O32- will be needed to react with the I3- that is generated?
2𝑚𝑜𝑙 𝑆2 𝑂32−
𝑚𝑜𝑙 𝑆2 𝑂32− = 8.75𝑥10−5 𝑚𝑜𝑙 𝐼3− 𝑥
1𝑚𝑜𝑙 𝐼3−
- 𝑚𝑜𝑙 𝑆2 𝑂32− = 1.75𝑥10−4 𝑚𝑜𝑙

d. And also, assuming the concentration of the S2O32- titrant to be 0.025 M How many
milliliters of titrant will be predictably used for the analysis.
1𝐿 1000𝑚𝑙
𝑉𝑁𝑎2𝑆2𝑂3 = 1.75𝑥10−4 𝑚𝑜𝑙 𝑆2 𝑂32− 𝑥 2− 𝑥
0.025𝑚𝑜𝑙𝑆2 𝑂3 1𝐿
- VNa2S2O3 = 7 mL
7. A nonscientist brings a water sample to your laboratory and asks you to determine why
there was a fish kill in the nearby lake. Having recently finished this experiment, what
might you tell that person about the legitimacy of as test for dissolved oxygen? What
reasoning would you use to maintain the integrity of your laboratory.
- One of the causes of fish kill in a certain place is oxygen depletion in the water body.
Although fishes can adjust to changes like change in temperature, there are times
where the increase of temperature will lead to having more algae in that place. With
more algae, there is competition for oxygen. Thus, oxygen depletion can be
experienced by the fishes.
8. A. Fish kills are often found near the discharge point of water from cooling waters at
electrical generating power plants. Explain why this occurrence may occur.
- Electric generating power plants need steam to turn their turbines so that electricity will
be generated. With that, fishes near the discharge point have a great tendency to die due
to a higher temperature of water when it is returned to its source.
B. Fish kills are often found in streams following heavy rainfall in a watershed dominated
by farmland or denuded forestland. Explain why this occurrence may occur.
- One of the main reasons why fish kills are found in streams near or dominated by
farmland is because of the sediments that goes or transfers to the body of water when
it is raining. These sediments can ruin the habitat or even the marine ecosystem in
that specific place.
9. Explain how the dissolved oxygen concentrations may change starting at the headwaters
of a river and ending at the ocean. Account for the changes.
- The value of the dissolved oxygen concentrations is decreasing starting from the
headwaters of a river and ending at the ocean. This is possibly because of the bacteria
present in the river, given that bacteria have a higher change of survival in rivers than
in salt water.
10. Salt (ocean) water generally has a lower dissolved oxygen concentration than freshwater
at a given temperature. Explain why this is generally observed.
- This is due to the fact that marine life is greater in the ocean than in freshwater, thus,
more animals are feeding oxygen in the ocean than in a freshwater
CONCLUSION
This experiment was carried out in the high hopes to learn a technique on how to obtain
natural water sample to determine the concentration of dissolved oxygen in a given sample of
water, and to learn the chemical reactions involved in fixing and analyzing a water sample for
dissolved oxygen using Winkler method. After successfully completing the experiment, the
following were learned in the process, the term “to fix” the dissolved oxygen in the sample means
to add a certain solution to make the oxygen fixed in the sample so that the oxygen content cannot
escape in the solution even when the cork will be removed in the flask. This was also done so as
to quantitate the dissolved oxygen for the computation analysis. The precipitate formed after
adding manganese (II) sulfate indicates that there is indeed a certain amount of dissolved oxygen
present in the water sample. Also, as the temperature increases, the dissolved oxygen concentration
decreases, thus, cold water bodies hold more oxygen than the warmer bodies. Lastly, the reaction
of the sample with thiosulfate from the titration process confirms the presence of the dissolved
oxygen concentration in the water sample.

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What does it mean to 'fix' the dissolved oxygen in a water sample?

How does temperature affect the dissolved oxygen concentration in water?

EXPERIMENT 6

Dissolved Oxygen Levels in Natural Water

Sofia Isabelle G. Garcia

In this experiment the Laboratory questions and their answers are:

- 𝑚𝑜𝑙 𝑆2 𝑂32− = 1.75𝑥10−4 𝑚𝑜𝑙

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