Lab Exercise 8 - Histology - Part 2: IV-C T

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LAB EXERCISE 8 – HISTOLOGY - PART 2

REQUIRED:
Reading required:
 Laboratory manual: Human Anatomy & Physiology Laboratory Manual with Rat Dissections, 3rd
Custom Edition for St. John's University, 2017. Read below for specific Exercises & Activities.

 Lab Guide - Laboratory Exercise # 8 (printout)

Personal Protective Equipment and required materials:


 White Lab coat (full knee length & long sleeves)

PRE LAB ASSIGNMENT:


Read the lab guide and familiarize yourself with the tissues and the various notes. Read pg. 75, 76
and see fig. 6.4 to go over the bolded terms.

In today’s lab, you will be provided with:


1. A Compound microscope
2. A set of microscope slides for Histology – Part 2

Usage of a Compound Microscope and Slide Care:


Before beginning this lab check with your laboratory assistant about the use and care of the compound
microscopes – remember to focus in with the coarse adjustment knob until you see your tissue, then you
should need only a slight turn of the fine adjustment knob to focus in the higher power lenses. ALWAYS
PLACE AND REMOVE THE SLIDE WITH THE 4X LENS IN PLACE.

CLASSIFICATION OF TISSUES:
Read Exercise 6, pages 67-92

IV- CONNECTIVE TISSUE (pg. 75-82)

5 Main Categories
Connective tissue types can be differentiated by the type of
1) Extra cellular matrix
2) Fibers
3) Cells
4) Vascularization.

Study the distinguishing features of each tissue type using the Human Anatomy & Physiology Laboratory
Manual, Pages 75-82, then look at the slide under the microscope to find these features. Identify the tissue
based on the presence or absence of the distinguishing features. Write down your observations.
DO NOT memorize the pictures!

1. EMBRYONIC connective tissue is a loose tissue formed during early embryonic development. This
type of connective tissue is found primarily in the umbilical cord. Embryonic connective tissue is not found
in adults. There are 4 types of connective tissue which are derived from an embryonic tissue called
mesenchyme.

1
Type Slide # List & Draw the Distinguishing Features

#30
Mesenchyme
Fig. 6.5(a), pg. 76

2. CONNECTIVE tissue (proper) is distinguish by their specific type of cells, fibers and ground substance

2.a. Loose connective tissue

Type Slide # List & Draw the Distinguishing Features

#31 - Areolar
Areolar
Fig. 6.5(b), pg. 77

#32 –
Adipose Adipose fat
Fig. 6.5(c), pg. 77 stained

#33 –
Adipose
OsO4 stained

#34 – Lymph
Reticular Node
Fig. 6.5(d), pg. 78

2
2.b. Dense connective tissue

Type Slide # List & Draw the Distinguishing Features

Dense #35 –
Tendon
Regular #36 –
Fig. 6.5(e), pg. 78 Ligament

Dense #1 – Skin
Irregular
Fig. 6.5(g), pg. 79

Elastic #42 – Aorta


Fig. 6.5(f), pg. 79 and Vein

3. CARTILAGE

Type Slide # List & Draw the Distinguishing Features

Hyaline #9 – Trachea
Fig. 6.5(h), pg. 80

Ask TA/TF for Fibrocartilage slide


Fibrocartilage #38 – White
Fig. 6.5(j), pg. 81 fibrocartilage

3
#37 – Elastic
Elastic Cartilage
Fig. 6.5(i), pg. 80

4. BONE
Type Slide # List & Draw the Distinguishing Features

#39 – Compact
Compact Bone (Dry-
Fig. 6.5(k), pg. ground)
81

#40 – Cancellous
Cancellous Bone
(Spongy)

5. BLOOD – Figure 6.5l (l), pg. 82. Slide #41 – Blood Smear
Read Exercise 29, pages 425-431
Table: 29.1, page 428 (different elements of blood)

List & Draw the Distinguishing Features


Erythrocytes/ Leukocytes/White blood Cells
Red Blood Granular Agranular Platelet
Cells Eosinophils Neutophils Basophils Lymphocyte Monocyte

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