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    And Deletions.: Additional File 6. List of Oligonucleotides Used in This Work For Construction of Motifs

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    Juan Yujra Cárdenas
    This document lists oligonucleotides used for constructing motifs and deletions in this work. It provides the name, sequence, and purpose of each primer listed. Restriction endonuclease sites were added after annealing complementary forward and reverse oligonucleotides.

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    © All Rights Reserved
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    And Deletions.: Additional File 6. List of Oligonucleotides Used in This Work For Construction of Motifs

    Uploaded by

    Juan Yujra Cárdenas
    10 views1 page
    This document lists oligonucleotides used for constructing motifs and deletions in this work. It provides the name, sequence, and purpose of each primer listed. Restriction endonuclease sites were added after annealing complementary forward and reverse oligonucleotides.

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    1471-2199-9-107-s6

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    This document lists oligonucleotides used for constructing motifs and deletions in this work. It provides the name, sequence, and purpose of each primer listed. Restriction endonuclease sites were added after annealing complementary forward and reverse oligonucleotides.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    10 views1 page

    And Deletions.: Additional File 6. List of Oligonucleotides Used in This Work For Construction of Motifs

    Uploaded by

    Juan Yujra Cárdenas
    This document lists oligonucleotides used for constructing motifs and deletions in this work. It provides the name, sequence, and purpose of each primer listed. Restriction endonuclease sites were added after annealing complementary forward and reverse oligonucleotides.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    You are on page 1of 1

    Additional file 6.

    List of oligonucleotides used in this work for construction of motifs

    and deletions.

    Primer name Sequence (from 5’ to 3’)


    ZFP1m(-) Fwd aattcAAAAAAAAGAATGAGCGACGTAAATa
    ZFP1m(-) Rev agcttATTTACGTCGCTCATTCTTTTTTTTg
    ZFP1m(+) Fwd AGTGCACATAACGAAAAAAAAAAAAGAA
    ZFP1m(+) Rev AACTCGTACCCAACACTCCAAAATAA
    ZFP1m Fwd aattcAAAAAAAAGAATCTGCATATATATATATATATAACATGGGAGCGACGTA
    AATa
    ZFP1m Rev agcttATTTACGTCGCTCCCATGTTATATATATATATATATGCAGATTCTTTTTTT
    Tg
    ZFP1m mut Fwd aattcAAAAAAAAGAAGGCGCCCCCCCCCCCCCCCCCCCCCCGCCAGCGACGTA
    AATa
    ZFP1m mut Rev agcttATTTACGTCGCTGGCGGGGGGGGGGGGGGGGGGGGGGCGCCTTCTTTTT
    TTTg
    RpS5m(+) Fwd TGAACGAGGCGATCTACCTAATGTG
    RpS5m(+) Rev CGTTCACAATCTCATCGGCAAGACA
    RPS5m Fwd aattcGATCTACCTAATGTGCAAGGGTGCCCGCGAGGCTGCTTTCCGCAAAATC
    ACGACa
    RPS5m Rev agcttGTCGTGATTTTGCGGAAAGCAGCCTCGCGGGCACCCTTGCACATTAGGT
    AGATCg
    RPS5m(-) Fwd aattcGATCTACCTAAATCACGACa
    RPS5m(-) Rev agcttGTCGTGATTTAGGTAGATCg
    RPS5m mut aattcTGAACGAGGCGATCTACCTACCTCAAGACACTGCCTGAGTGTCTTGCCG
    Fwd ATGAGATTGTGAACGg
    RPS5m mut Rev agcttGTTCACAATCTCATCGGCAAGACACTCAGGCAGTGTCTTGAGGTAGGTA
    GATCGCCTCGTTCAg
    PARB1ab Fwd AATGAAAGGCAAAAAGAAAG
    PARB1-a Rev CGAGACACCCCAACTGACAGGA
    PARB1-b Rev TTTACTGGAGCACAGTTTCC

    Restriction endonuclease sites (lower case) were generated after annealing of synthetic
    complementary forward (Fwd) and reverse (Rev) oligonucleotides.

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