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    Supplementary Table S1

    Strain Relevant features Reference


    Escherichia coli
    DH5α F- φ80lacZΔM15, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(rK-m Hanahan, 1983
    K-), supE44, thi-1, gyrA, relA1
    DH5αλpir F- φ80lacZΔM15, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(rK-m Martínez-
    K-), supE44, thi-1, gyrA, relA1, λpir lysogen García & de
    Lorenzo, 2011
    Acinetobacter baumannii
    AB5075 Wild type strain Jacobs et al.,
    2014
    ΔcmlA5 AB5075 derivative, in-frame deletion in cmlA5 (ABUW_4059) This work
    ΔcraA AB5075 derivative, in-frame deletion in craA (ABUW_0337) This work
    p1' AB5075 derivative, indeterminate rearrangement in p1AB5075 This work
    loosing cmlA5 region (likely the whole resistance island 2)
    Δp1AB507 AB5075 derivative, cured from p1AB5075 plasmid This work
    5
    Plasmids Relevant features Reference
    pEMG KmR, oriR6K, lacZa with two flanking I-SceI sites Martínez-
    García & de
    Lorenzo, 2011
    pEMGT- pEMGT with upstream and downstream homologous regions for This work
    cmlA5 deletion of cmlA5
    pEMGT- pEMGT with upstream and downstream homologous regions for This work
    craA deletion of craA
    pEMG-Tel KmR, TelR, oriR6K, lacZa with two flanking I-SceI sites This work
    (pEMGT)
    pFLAG-attP AprR, CmR, attP integration site (Addgene, #110095) Unpublished
    pMo130- KmR, TelR, sacB, xylE Amin et al.,
    TelR 2013
    pRK2013 KmR, ColE1, tra+. Conjugative helper plasmid. Figurski and
    Helinski, 1979
    pSEVA524 TcR, oriRK2, lacIq-Ptrc Silva-Rocha et
    al., 2013
    pSW-Apr AprR, oriRK2, xylS, Pm→sceI (transcriptional fusion of I-sceI to Pm) This work
    pSW-I ApR, oriRK2, xylS, Pm→sceI (transcriptional fusion of I-sceI to Pm) Martínez-
    García & de
    Lorenzo, 2011
    pSW-Tc TcR, oriRK2, xylS, Pm→sceI (transcriptional fusion of I-sceI to Pm) This work
    Primer Sequence Reference
    Apr fw AGAGGAGACCTAGTTGGCAC This work
    Apr rv GAGCTGAAGAAAGACAATCC This work
    cmlA5 down fw GATGGTTTCGTGCGCTCAAAACGTTGAGAGAATGTGGCAAG This work
    cmlA5 down rv GGAAGCAGGTGCGATTTTCG This work
    cmlA5 rv seq ATCAATGTCGATCATGGCTG This work
    cmlA5 up fw AGTTGACATAAGCCTGTTCGG This work
    cmlA5 up rv TTTTGAGCGCACGAAACCATC This work
    crA up fw CATTTAATTCACCATTTGAG This work
    craA down fw GAAGTGTACAAACTCATGAAAAGAGTTGCACAAGACTTAC This work
    craA down rv CTATTTAGACATGCAGTACG This work
    Supplementary Table S1

    craA fw seq CGAGGAAGCAGCAGACTTTC This work


    craA up rv TTCATGAGTTTGTACACTTC This work
    oSA67 GACGCCCGTCTAACAATTCG Anderson et al.,
    2020
    oSA68 CCCCTCGATGGAAGGGTTA Anderson et al.,
    2020
    oSA86 TAAGCGTCAGGCAGACAAG Anderson et al.,
    2020
    oSA87 TTTTCCACTCTGCTGAAGG Anderson et al.,
    2020
    tetA fw TAAATTTGACAGCTTATCATCG This work
    tetA rv AAAGGACAATTGTCTCAGGTCG This work

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