2-UV-Vis Molecular Spectrometry
2-UV-Vis Molecular Spectrometry
2-UV-Vis Molecular Spectrometry
Spectrophotometry
Review
This lamp contains xenon at a fairly high pressure and the light is
formed via a discharge across a pair of electrodes.
A continuous UV and Vis emission is emitted due to the presence of
the xenon.
Its wavelength output ranges from 180-2500 nm
The intensity varies with wavelength
2. Wavelength selection
The most inexpensive way to isolate a wavelength band is with the use of
absorption filters.
For a visible light, such a filter would consist of colored glass, the color of the glass
indicating what region of the visible spectrum is passed.
Thus, if the wavelength called for a given method is in the red region of the visible
spectrum, a red colored glass filter is chosen
Absorption filters have the lowest
resolution, having effective
bandwidths varying
from 30-250 nm.
2.2. Monochromators
Photomultiplier tubes
A photomultiplier tube is a light sensor combined with a signal amplifier.
The light emerging from the sample compartment strikes the photosensitive
surface and the resulting electrical signal is amplified
Deviations from Beer’s law are evidence when the Beer’s law plot is
not linear.
This is probably most often observed at the higher concentrations
of the analyte.
Such deviations can be:
chemical
instrumental
Instrumental deviations
Wavelength selection
in order to realize maximum sensitivity, spectrophotometric absorbance
measurements are usually made at the wavelength of maximum absorption.
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