00-0205 APB ÄKTA BROSCH 00-06-21 15.

42 Sidan 12

One platform
simplifies all your
purification tasks.
ÄKTAdesign.
Real progress in
biomolecule purification.

18-1137-79
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ÄKTAdesign.
What type of work
are you doing?
What type of purification is UNICORN
going on in your lab? for Windows NT: no other control
Are you developing protocols and optimizing methods system is this user friendly.
for biomolecule purification? Are you purifying synthetic Conducting and controlling purification tasks couldn’t
peptides or nucleic acids? Native or recombinant proteins? be simpler. From planning to final report, from lab to
production scale, UNICORN™ for Windows™ NT gives
If your focus is fast method and process development
you choices all the way.
of any biomolecule ÄKTA™explorer is ideal, while
ÄKTApurifier is designed for those who routinely purify Need to modify your system configuration? No problem.
and characterize peptides and nucleic acids. ÄKTA FPLC™ New flexible system software lets you add a new function

is optimized for research and development with proteins. and UNICORN will do the rest.

And if you need a compact, ready-to-use, push-button And as you scale-up your work UNICORN ensures that
solution for simple protein purification, ÄKTAprime your pilot- and production-scale protocols will operate as
is the answer. smoothly as your initial purification strategies.

With unique networking capabilities, UNICORN gives

One platform from basic research
you total control – from your desk.
to process development.
Äkta is the Swedish word meaning true; actual; real. New Frac-950: no other fraction
ÄKTA design is the name for a platform of systems, collector is this flexible.
control software and pre-packed columns that help you Frac-950 offers spill-proof fraction
make real progress in biomolecule purification. collection across the entire flow rate
And you’ll save time, because ÄKTA design allows everyone range of ÄKTA design systems.
to work in the same, logical fashion. ÄKTA design gives you From 1 µl/min to 100 ml/min,
fast access to a host of pre-tested purification protocols, from microplates to large vessels,
method templates, automatic buffer preparation, and a the choice is yours.
complete range of pre-packed columns. Whatever your
application or scale of purification, you will have the
fastest, simplest approach to purification. With ÄKTAdesign,
you can perform any type of purification with speed,
ease and confidence.
00-0205 APB ÄKTA BROSCH 00-06-21 15.42 Sidan 8

ÄKTAprime:
simple purification of proteins.

ÄKTA prime is ideal whenever, and

wherever, a simple, rapid protein
purification is needed in the laboratory.
Compared to traditional manual
operations, ÄKTAprime offers significant
advantages in speed and capacity.

No matter whether the task is a quick

sample clean-up or a purification
from a large volume of cell culture
supernatant, this compact system
will be ready to use.

With pre-installed application templates

and pre-packed columns, ÄKTA prime
performs the most common purification
steps at the touch of a button.

Pure protein at the touch of a button.

00-0205 APB ÄKTA BROSCH 00-06-21 15.42 Sidan 9

ÄKTAFPLC:
high performance purification
of proteins.

ÄKTA FPLC builds on the world-wide

success of FPLC™ system, but offers
major advantages in terms of design
and versatility.This high performance
system sets a new standard in reliable
protein purification.

ÄKTA FPLC offers the choice between

one-step procedures or automated
multi-column purification schemes to
purify proteins in microgram to milligram
scale at flow rates up to 20 ml/min.

The innovative, compact design makes

the system extremely easy to work
with, without losing flexibility.The
integral rack opens to accommodate
any column. New functions and
equipment, such as automatic sample
application with Pump P-950 or the
spill-proof fraction collector Frac-950,
are simple to add and control.

mAU mS/cm)

40.0

50

20.0

0
0.0 1.0 2.0 min

Fast separations are easy on ÄKTA FPLC. The integral rack of ÄKTA FPLC opens to allow The same, tried-and-tested pump technology
a wide variety of configurations. that you’ll find in FPLC – but now with twice
the throughput.
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00-0205 APB ÄKTA BROSCH 00-06-21 15.41 Sidan 3

ÄKTApurifier:
high performance purification
and characterization of
peptides and nucleic acids.

For fast, reliable separations of peptides and

nucleic acids at laboratory scale, ÄKTA purifier
is unbeatable. It is equally at home with routine
procedures of any complexity, with scouting
and method optimization, and with biomolecule
characterization, such as peptide mapping.

The system performs all chromatographic

techniques, helping you to scout for optimal
conditions for binding and elution, pH,
gradient shapes and flow rates. Pre-
programmed application protocols and
method templates ensure a fast and
easy start.The on-line adviser will
also provide advice on media selection,
buffers and methods.

And if your needs change, perhaps

you want a sample pump or the new
Frac-950 , simply add the new function
and let UNICORN take control.

mAU
Sephasil™ Peptide C18 5 µ ST 4.6/250
,
Tryptic digest RVP ovalbumin
Flow rate 1 ml/min
CH3CN/TFA

100

50

0 20 40 60 80 100 120 ml

The highest reproducibility – essential for

peptide mapping.
00-0205 APB ÄKTA BROSCH 00-06-21 15.41 Sidan 4

ÄKTAexplorer:
fast method and process
development and scale-up
for proteins, peptides
and nucleic acids.
ÄKTA explorer is designed for all
chromatographic techniques employed
in method and process development,
offering microgram to gram scale
purification. Developing optimized
methods is fast and easy.The knowledge
of the most experienced chromato-
graphers lies within the method
templates of ÄKTAexplorer.The scouting
function of UNICORN automatically
varies parameters over repeated runs.
BufferPrep calculates and prepares the
correct pH buffers from stock solutions,
while automatically compensating for
changes in temperature and salt
concentration.

With the new Pump P-950 and Frac-950

even the largest sample volumes can be
applied at flow rates up to 50 ml/min
and fractions collected, without spillage,
at flow rates up to 100 ml/min.

Method Optimization: Method/Process Development: Purification and scaling up:

• BufferPrep for accurate pH scouting • Column and media scouting • Scalability from µg to g scale
• Pre-programmed protocols • Pre-programmed protocols • Flow rate range –
• BufferPrep for automatic buffer preparation 10 µl/min to 100 ml/min
START CONDITIONS Run 1 Run 2 Run 3 Run 4 Run 5 Run 6 Run 7 Run 2 Run 3 Run 4
START CONDITIONS Run 1
pH 7 6.5 6 4.5
5.5 5 4.5 4
Column position Position 8 Position 7 Position 6 Position 5

mAU280 nm Conductivity (mS/cm) AU 280 nm Conductivity

mAU280nm Conductivity (mS/cm) (mS/cm)
b)
80.0 2.00
250 400
Conductivity 150

200 100
60.0 300

100
150
pH 4.5
200 1.00
40.0
50
100
Fab
50
100
50 20.0

0 0
0
0 0 500 1000 1500 2000 2500
2.0 4.0 6.0 8.0 Time (min) 0.0 10.0 Time (min) Volume (ml)

Automatic pH scouting on RESOURCE™ 6 ml HIC media scouting using HiTrap™ HIC Test kit. Five Optimized HIC separation on a 200 ml column.
prepacked column using BufferPrep.The scouting different media were tested under identical
scheme in UNICORN is shown on top. conditions. The scouting scheme to select media is
shown (upper). A simple valve switch was used to
select a new column for each run.The actual salt
gradient, monitored on-line by Monitor pH/C-900,
is depicted for the lower UV curve.
00-0205 APB ÄKTA BROSCH 00-06-21 15.42 Sidan 6

UNICORN for Windows NT.

Any purification task. Every
purification scale. One faster
more intelligent way of working.
Supplied as standard with all computer controlled systems UNICORN offers functions that are vital in today’s
in the ÄKTA design family, UNICORN gives you a R&D laboratories and production facilities. Pre-
common control platform and one common user interface programmed method templates and scouting functions
for all scales of operation.This unique control system simplify purification tasks, while the ability to generate
software with its networking capabilities puts you in total customized reports and documentation saves time.
control throughout your facility – from lab-scale research UNICORN is also supplied with comprehensive
to full-scale production. From planning to final report, documentation that helps to fulfil regulatory requirements.

Networking:
Remote Control/overview
PC from any PC

PC Network
Server

Local Local
PC PC
Share data

System System System System System System System System

1 2 3 4 5 6 7 8

Location 1 Location 2
Connect any UNICORN
controlled system
00-0205 APB ÄKTA BROSCH 00-06-21 15.42 Sidan 7

Start button

Select system A new method

is created in less
than 3 minutes.
Select technique

Select template

Select column

Run

User defined numerical

data display

While a method is running,

you have full overview of all
system parameters and direct
access to manual instructions
User defined through the interactive flow
graphical data schemes and run data.
display

System flow path

The evaluation module

will help you analyse your
results and present them
the way you want.
00-0205 APB ÄKTA BROSCH 00-06-21 15.41 Sidan 2

Purification
HiTrap™ Chelating
AU280nm

1.0

0.5

High Resolution Analysis

SOURCE™ 5 RPC
mAU256nm %B 0.0

200 18 20 80 Flow through Wash Eluted

pool

15 19
0.0 5.0 10.0 15.0 20.0 Vol. (ml)
150 60

100 40

50 20
Affinity
0 0
High Resolution Separation
10.0 15.0 20.0 min Superdex™ 200
mAU

800

600 214 nm

400

280 nm
200

0
Hydrophobicity 0 5 10 15 20 ml

Media Screening
HiTrap HIC test kit Size
mAU280nm Conductivity (mS/cm)

400
Conductivity 150

300

100

200
Sample Clean-Up
HiPrep™ 26/10 Desalting
Fab
100
50
Charge AU280 nm Conductivity (mS/cm)

Method Scouting 0.25

0
0 RESOURCE™ S 6 ml
0.0 10.0 Time (min)
mAU280 nm Conductivity (mS/cm) 0.20
10.0

Conductivity
80.0
0.15
250

0.10
200 5.0
60.0

0.05

150
pH 4.5
0.00
40.0 0.0 1.0 2.0 Time (min)

100

50 20.0

2.0 4.0 6.0 8.0 Time (min)

00-0205 APB ÄKTA BROSCH 00-06-21 15.42 Sidan 10

The right combination

is the key to success
A successful purification can be guaranteed by using the
techniques, columns and media in the most efficient
combination on the best system.

ÄKTA design uses the widest range of columns and media.

There are hundreds of columns available to cover
purification techniques such as:

• ion exchange chromatography

• reversed phase chromatography

• size exclusion chromatography

• affinity chromatography

• hydrophobic interaction chromatography.

ÄKTA design ensures that any pre-packed column is run

correctly and even assists you in choosing the best columns
and media to optimize your purification.

At Amersham Pharmacia Biotech, you will find the widest

range of columns and media available. By using our
extensive experience, we help you to combine each
purification step into an efficient and effective strategy,
for any purification task, at any scale.
00-0205 APB ÄKTA BROSCH 00-06-21 15.42 Sidan 11

For more information about

the complete ÄKTAdesign range,
see the ÄKTA homepage:
www.apbiotech.com/akta
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Fax: 09 512 1710

ÄKTA, UNICORN, FPLC, SOURCE, RESOURCE, Sephasil, Superdex, HiPrep and HiTrap
are trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham plc. Pharmacia and Drop Design
are trademarks of Pharmacia & Upjohn Inc.
Windows NT is a trademark of Microsoft Corporation.
All goods and services are sold subject to the terms and conditions of sale of the
company within the Amersham Pharmacia Biotech group which supplies them.
A copy of terms and conditions is available on request.
© Amersham Pharmacia Biotech AB 2000 – All rights reserved.
Amersham Pharmacia Biotech UK Limited Amersham Place
Little Chalfont Buckinghamshire England HP7 9NA
Amersham Pharmacia Biotech AB SE-751 84 Uppsala Sweden
Amersham Pharmacia Biotech Inc 800 Centennial Avenue
PO Box 1327 Piscataway NJ 08855 USA
Amersham Pharmacia Biotech Europe GmbH
Munzinger Strasse 9 D-79111 Freiburg Germany