Pollen Grain: The Pollen Grains Represent The Male Gametophytes

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DAY-6 DATE- 14.04.

2020

Pollen grain: The pollen grains represent the male gametophytes.

Pollen grains are generally spherical measuring about 25-50 micrometers in diameter.

It has a prominent two-layered wall.

The hard outer layer called the exine is made up of sporopollenin which is one of the most resistant
organic material known.

It can withstand high temperatures and strong acids and alkali. No enzyme that degrades
sporopollenin is so far known.

Pollen grain exine has prominent apertures called germ pores where sporopollenin is absent.

Pollen grains are well preserved as fossils because of the presence of sporopollenin.

The exine exhibits a fascinating array of patterns and designs.


The inner wall of the pollen grain is called the intine.

It is a thin and continuous layer made up of cellulose and pectin.

The cytoplasm of pollen grain is surrounded by a plasma membrane. When the pollen grain is
mature it contains two cells, the vegetative cell and generative cell .

The vegetative cell is bigger, has abundant food reserve and a large irregularly shaped nucleus

The generative cell is small and floats in the cytoplasm of the vegetative cell. It is spindle shaped
with dense cytoplasm and a nucleus.

In over 60 per cent of angiosperms, pollen grains are shed at this 2-celled stage. In the remaining
species, the generative cell divides mitotically to give rise to the two male gametes before pollen

grains are shed (3-celled stage).


Pollen grains of many species cause severe allergies and bronchial afflictions in some people often
leading to chronic respiratory disorders– asthma, bronchitis, etc. It may be mentioned that
Parthenium or carrot grass that came into India as a contaminant with imported wheat, has become
ubiquitous in occurrence and causes pollen allergy

Pollen grains are rich in nutrients. It has become a fashion in recent years to use pollen tablets as
food supplements. In western countries, a large number of pollen products in the form of tablets
and syrups are available in the market. Pollen consumption has been claimed to increase the
performance of athletes and race horses.

When once they are shed, pollen grains have to land on the stigma before they lose viability if they
have to bring about fertilisation. The period for which pollen grains remain viable is highly variable
and to some extent depends on the prevailing temperature and humidity. In some cereals such as
rice and wheat, pollen grains lose viability within 30 minutes of their release, and in some members
of Rosaceae, Leguminoseae and Solanaceae, they maintain viability for months.

You may have heard of storing semen/ sperms of many animals including humans for artificial
insemination. It is possible to store pollen grains of a large number of species for years in liquid
nitrogen (-1960C). Such stored pollen can be used as pollen banks, similar to seed banks, in crop
breeding programmes.

The Pistil, Megasporangium (ovule) and Embryo sac

gynoecium represents the female reproductive part of the flower.

gynoecium may consist of a single pistil (monocarpellary)

or may have more than one pistil (multicarpellary). When there are more than one, the pistils may

be fused together (syncarpous) or may be free (apocarpous)


Each pistil has three parts ,the stigma, style and ovary.

The stigma serves as a landing platform for pollen grains. The style is the elongated slender part
beneath the stigma. The basal bulged part of the pistil is the ovary. Inside the ovary is the ovarian
cavity (locule). The placenta is located inside the ovarian cavity.

Arising from the placenta are the megasporangia, commonly called ovules.

The number of ovules in an ovary may be one (wheat, paddy, mango) to many (papaya, water
melon, orchids).

The Megasporangium (Ovule) :

The ovule is a small structure attached to the placenta by means of a stalk called funicle. The body of
the ovule fuses with funicle in the region called hilum. Thus, hilum represents the junction between
ovule and funicle. Each ovule has one or two protective envelopes called integuments. Integuments
encircle the ovule except at the tip where a small opening called the micropyle is organised.
Opposite the micropylar end, is the chalaza, representing the basal part of the ovule.
Enclosed within the integuments is a mass of cells called the nucellus. Cells of the nucellus have
abundant reserve food materials. Located in the nucellus is the embryo sac or female gametophyte.
An ovule generally has a single embryo sac formed from a megaspore through reduction division.

Megasporogenesis : The process of formation of megaspores from the megaspore mother cell is
called megasporogenesis.

Ovules generally differentiate a single megaspore mother cell (MMC) in the micropylar region of the
nucellus . It is a large cell containing dense cytoplasm and a prominent nucleus. The MMC undergoes
meiotic division. Meiosis results in the production of four megaspores

Female gametophyte : In a majority of flowering plants, one of the megaspores is functional while
the other three degenerate. Only the functional megaspore develops into the female gametophyte
(embryo sac).

WORKSHEET

1. It is the tissue in the ovary on which the ovules develop and are attached to the ovary wall .Name
this tissue located inside the ovarian cavity.

2 With a neat, labelled diagram, describe the parts of a typical angiosperm ovule.

3.Identify A&B and write their fate.

4. (a) "The microspore is haploid while that of microspore mother cell is diploid". Comment.
(b) How many male gametes and female gametes are produced by-

(i) Five microspore mother cells (ii) Five megaspore mother cells

5. Comment on the following diagrams-

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