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    How To Take Sputum Suitable For TB - Inspection

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    GeneXpert Lab LCP
    This document provides instructions for collecting sputum samples for tuberculosis testing. It outlines the following steps: collect sputum in the morning after relaxing and taking a deep breath; cough vigorously into a designated container to collect at least 2mL of sputum; and check that purulent sputum was collected before tightly capping and refrigerating the sample for up to 72 hours or freezing at -20°C if only nucleic acid testing will be done. Good sputum samples contain at least 1/3 purulent parts, while poor samples are mostly mucus, blood, or saliva.

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    How To Take Sputum Suitable For TB - Inspection

    Uploaded by

    GeneXpert Lab LCP
    295 views1 page
    This document provides instructions for collecting sputum samples for tuberculosis testing. It outlines the following steps: collect sputum in the morning after relaxing and taking a deep breath; cough vigorously into a designated container to collect at least 2mL of sputum; and check that purulent sputum was collected before tightly capping and refrigerating the sample for up to 72 hours or freezing at -20°C if only nucleic acid testing will be done. Good sputum samples contain at least 1/3 purulent parts, while poor samples are mostly mucus, blood, or saliva.

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    How to take sputum suitable for TB_inspection

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    This document provides instructions for collecting sputum samples for tuberculosis testing. It outlines the following steps: collect sputum in the morning after relaxing and taking a deep breath; cough vigorously into a designated container to collect at least 2mL of sputum; and check that purulent sputum was collected before tightly capping and refrigerating the sample for up to 72 hours or freezing at -20°C if only nucleic acid testing will be done. Good sputum samples contain at least 1/3 purulent parts, while poor samples are mostly mucus, blood, or saliva.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    295 views1 page

    How To Take Sputum Suitable For TB - Inspection

    Uploaded by

    GeneXpert Lab LCP
    This document provides instructions for collecting sputum samples for tuberculosis testing. It outlines the following steps: collect sputum in the morning after relaxing and taking a deep breath; cough vigorously into a designated container to collect at least 2mL of sputum; and check that purulent sputum was collected before tightly capping and refrigerating the sample for up to 72 hours or freezing at -20°C if only nucleic acid testing will be done. Good sputum samples contain at least 1/3 purulent parts, while poor samples are mostly mucus, blood, or saliva.

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    Sputum Collection Method for Tuberculosis

    (Acid-Fast Bacterium) Testing


    Procedure for Sputum expectoration
    * Because there is a risk of contagion, sputum collection should be performed
    in a room with no other people, and secure good ventilation (natural of
    machinery).
     Collect sputum when the patient wakes up in the morning.
     Relax the patient and take a deep breath.
     Expectorate sputum with a vigorous cough.
     Collect sputum as much as possible in the designated container (at least 2mL).
     Check whether you succeeded in collecting “good sputum (sputum with pus)”.
     Close the cap of the container tightly and submit the sample for testing as soon as possible. If you
    cannot submit it right away, please store it in a refrigerator (up to 72 hours).
    * If the sample is left in a warm place (at room temperature or above) for a long period of time, sundry bacteria will proliferate, and
    it may not be possible to obtain an accurate result.
    * If testing is performed solely by the nucleic acid amplification method, frozen storage of the samples (-20˚C) is also possible.
    * Do not freeze the sample if culture tests are to be performed.

    Examples of sputum – Good and Poor


    Good sputum Poor sputum
    * The good sputum is to be fresh with much purulent part. * It will be difficult to expect highly sensitive result with these types of sputum.

    Purulent sputum with Saliva or sputum


    purulent part accounting consisting completely of
    for 1/3 or less*1 mucous*1

    Purulent sputum with Mucous sputum containing


    purulent part accounting a small amount of purulent
    for 1/3 to 2/3*1 sputum*1

    Bloody sputum
    Purulent sputum with (Haemosputum)
    purulent part accounting
    for 2/3 or more*1*
    (Ha

    *1: Miller & Jones classification


    Editorial supervision: Prof. Satoshi Mitarai, Head, Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-tuberculosis
    Association

    Eiken Chemical Co., Ltd. 8120 AK2MK


    Created March 2014
    143 Nogi, Nogi-machi, Shimotsuga-gun, Tochigi Prefecture 329-0114

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