Protein Synthesis Worksheet PART A. Read The Following

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PROTEIN SYNTHESIS WORKSHEET

PART A. Read the following:


Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called
Transcription. It occurs in the nucleus. During transcription, mRNA transcribes (copies) DNA. DNA is
“unzipped” and the mRNA strand copies a strand of DNA. Once it does this, mRNA leaves the nucleus and
goes into the cytoplasm. mRNA will then attach itself to a ribosome. The strand of mRNA is then read in order
to make protein. They are read 3 bases at a time. These bases are called codons. tRNA is the fetching puppy.
It brings the amino acids to the ribosome to help make the protein. The 3 bases on tRNA are called anti-codons.
Remember, amino acids are the building blocks for protein. On the mRNA strand, there are start and stop
codons. Your body knows where to start and stop making certain proteins. Just like when we read a sentence,
we know when to start reading by the capitalized word and when to stop by the period.

Ribosome mRNA DNA


tRNA
mRNA

PART B. Answer the following questions on your paper:

1. What is the first step of protein synthesis? Transcription


2. What is the second step of protein synthesis? Translation
3. Where does the first step of protein synthesis occur? nucleus
4. Where does the second step of protein synthesis occur? ribosome
5. Nitrogen bases are read 3 bases at a time.
6. The bases on the mRNA strand are called codons
7. The bases on tRNA are called anticodon.
8. What is a start codon? marks the site at which translation into protein sequence begins
9. What is a stop codon? marks the site at which translation ends.
10. A bunch of amino acids attached together is called a protein.
PART C. Use your codon chart or genetic code to determine the amino acid sequence. Remember to
read through the strand and ONLY start on AUG and STOP when it tells you to stop. Follow example
below:

Example:
DNA AGA CGG TAC CTC CGG TGG GTG CTT GTC TGT ATC CTT CTC AGT ATC
mRNA UCU GCC AUG GAG GCC ACC CAC GAA CAG ACA UAG GAA GAG UCA UAG
protein start - glu – ala –thre – hist – asp –glu – threo - stop
acid acid

1. DNA 🡪 CCT CTT TAC ACA CGG AGG GTA CGC TAT TCT ATG ATT ACA CGG TTG CGA TCC ATA ATC
mRNA 🡪 GGA GAA AUG UGU GCC UCC CAU CCG AUA AGA UAC UAA UGU GCC AAC GCU AGG UAU UAG
protein 🡪 start–cys–ala–ser–his–pro–iso–arg–tyro-stop

2. DNA 🡪 AGA ACA TAA TAC CTC TTA ACA CTC TAA AGA CCA GCA CTC CGA TGA ACT GGA GCA
mRNA 🡪 UCU UGU AUU AUG GAG AUU UGU GAG AUU UCU GGU CGU GAG GCU ACU UGA CCU CGU
protein 🡪 start-glu–iso–cyst–glu-iso-ser-gly-arg-glu-ala-thre-stop

3. DNA 🡪 TAC CTT GGG GAA TAT ACA CGC TGG CTT CGA TGA ATC CGT ACG GTA CTC GCC ATC
mRNA 🡪 AUG GAA CCC CUU AUA UGU GCG ACC GAA GCU ACU UAG GCA UGC CAU GAG CGG UAG
protein 🡪 start-glu-pro-leu-iso-cyst-ala-thre-glu-ala-thre-stop

4. DNA 🡪 TAA ACT CGG TAC CTA GCT TAG ATC TAA TTA CCC ATC
mRNA 🡪 AUU UGA GCC AUG GAU CGA AUC UAG AUU AAU GGG UAG
protein 🡪 start-aspa-arg-iso-stop

5. DNA 🡪 CTA TTA CGA TAC TAG AGC GAA TAG AAA CTT ATC ATC
mRNA 🡪 GAU AAU GCU AUG AUC UCG CUU AUC UUU GAA UAG UAG
protein 🡪 start-iso-ser-leu-iso-pheny-glu-stop

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