Republic of the Philippines

Region IV-A CALABARZON

Division of Quezon
DOMINICAN ACADEMY
Unisan, Quezon

THE EFFECTIVENESS OF TUBANG ASO LEAVES EXTRACT AS AN ALTERNATIVE

TOILET CLEANSER

A Science Investigatory Project Presented to the Faculty of Dominican Academy

Unisan, Quezon

In Partial Fulfilment of the Requirements of the Subject Research in Daily Life II

By

VON RICH M. GENEROSO

Proponent

March 2021
 ACKNOWLEDGEMENT

With deepest gratitude and sincere appreciation, I would like to extend an utmost

gratitude to the persons who, with all they can helped me in making this humble piece of

accomplishment be realized and be made a possible and successful one.

First and foremost, to Almighty God for the Divine guidance He has bestowed upon

me. I will forever be grateful and faithful;

To our School Directress/ Principal, Sr. Editha C. Alpuerto, O.P. for the untiring

support and words of wisdom that she have imparted to us;

To our Research Adviser, Ms. Genny E. Arroyo, for the guidance, and

encouragement to make this undertaking more beneficial;

To our Class Adviser, Madam Shiala I. De Rosas, for the friendly criticisms,

motivation, and untiring support given to me and my classmates;

To my friends as well as to my partner in life, for being always there for me

through thick and thin; and most importantly,

To my Family, a strong and gentle souls who taught me and made me become what

I am today, and for supporting me all throughout my journey.

THANKS TO YOU ALL!

-The Proponent
 DEDICATION

The proponent would like to dedicate this Science Investigatory Project first and

foremost to Almighty God;

To my dearest family; Mama, Itay, Kuya Jude, Kuya Mac, and baby Thea. I will

always offer to them my humble piece of accomplishments;

To my school, Dominican Academy. An institution who molds me to become a better

person, to love God and my neighbor, and inculcated values to become morally upright

individual;

Nevertheless, this piece of humble work is dedicated to every family, teachers,

entrepreneurs, and those in the field of manufactures for this will benefit them in all

facets of life;

Lastly, I dedicate this humble piece of accomplishment to myself for this will serve

as a fruit of my hard work and perseverance.

-The Proponent
ABSTRACT

In today’s generation, it is hard to invent instant things in our life and it is very crucial

to understand the importance of inventions or proposals that will surely benefit the

society. Thus, this study aims to understand the importance of natural product and the

significance of the manual process in order to come up with the natural product as an

alternative solution for daily home consumption. However, it is made to be mindful in

terms of decision making on what we are going to choose over basic and not.

This investigatory project provides a study to determine the advantages,

disadvantages, and impact of using natural product named Tubang Aso Leaves Extract

as an Alternative Toilet Cleanser.

The findings of this study indicate the good impact of natural product and its benefit

in all facets of life; especially now that we are in the midst of global crisis brought by

COVID-19 pandemic.
INTRODUCTION

Large amount of germs is mostly founded in toilet house, particularly toilets in

public establishments. Billions of microorganisms surrounded us that cannot be seen by

human’s eye and it can cause several illnesses. To prevent these, the product

antimicrobial toilet cleanser can help to kill and stop microbes to grow. Toilet bowl

cleaner is an effective acid-based stain remover that can remove soil, rust, and slime

from tiles, urinals, grouts, and toilet bowls. Tubang aso is a smooth, glamorous, erect,

branched shrub 2 to 5 meters high. Branches are stout, cylindric, and green. Leaves are

entire, orbicular-ovate, angular or somewhat 3- to 5-lobed, 10 to 18 centimeters long,

culminate with a chordate base. Petioles are long. In this study, with the help of

antimicrobial, tubang aso can be used as an agent that kills microorganisms and stops

their growth. It can be classified as disinfectant that kill a wide range of microbes on non-

living surfaces to prevent the spread of illness. Hence, this study was conducted to

identify the presence of natural antimicrobial compounds in Tubang Aso Leaf Extract.

The product toilet cleanser will use as an antimicrobial. According to the tests at the

University of Arizona by Kristen Buckles, ET. Al (2015), revealed that 62 percent of

bathroom floors were contaminated with potentially dangerous bacteria and 19 percent

of toilet bowls. Nonetheless, toilet bowls can contain some nasty bacteria. It means, it is

true that there are many nasty bacteria that can affect humans by having diseases.

Toilet cleanser is one of the good product that can help to have a safe and clean

bathroom especially in public establishment that many people used.

This purpose of this study is to understand the importance of natural product and

the significance of the manual process in order to come up with the natural product as

an alternative solution for daily home consumption.

1
Statement of the Problem

This study aspired to make an alternative toilet cleanser using Tubang Aso

(Jatropha Curcas) Leaves Extract so that it won’t be placed into waste. It aims to

develop a toilet cleanser with anti-microbial properties. Specifically, it seeks to answer

the following questions:

1. Is the Tubang Aso leaves extract effective as anti-microbial solution?

2. Is there a significant difference between Tubang Aso leaf extract toilet bowl

cleanser and commercial toilet bowl cleanser?

Hypothesis:

Stated below are the null and alternative hypotheses, which can answer the

statement of the problem.

1. Ho: The Tubang Aso Extract can’t be effective as anti-microbial solution.

Ha: The Tubang Aso Extract can be effective as anti-microbial solution.

2. There is no difference on the anti- microbial property of the ordinary Tubang

Aso leaves extract toilet bowl cleanser and commercial toilet bowl cleanser.

Ho: The Tubang Aso Extract can’t be a toilet cleanser.

Ha: The Tubang Aso Extract can be a toilet cleanser.

SIGNIFICANCE OF THE STUDY

The findings of this study, The Effectiveness of Tubang Aso Leaves Extract as an

Alternative Toilet Cleanser was deemed beneficial to the following:

The School Administrators will benefit from this study to prevent the spread of

illness and health problems as they utilize the natural product in eradicating microbes in

restroom facilities.

The findings of this study will be beneficial also to the community for many people

uses public toilets and as a result, they tend to adapt different microbes from the toilet. In

addressing these problems, Tubang Aso Leaves Extract as toilet cleanser can help to

remove microorganisms that can be found in various public toilets.

The Family. The findings of this study will be significant to every family for it will

serve as their alternative solution in cleaning their toilets at home.

Nevertheless, the result of this study will be beneficial to all manufacturers and

consumers of commercial products for sanitation. Thus, this study will add to the

growing invention in the field of hygienic products.

Most importantly, this study will be beneficial to the future Proponents. This study

will serves as a resource material for future researcher as reference data in conducting

new researches or in testing the validity of other related findings.

DEFINITION OF TERMS

To ensure a clearer and easier understanding of the study, the followings terms

were operationally defined:

Tubang Aso (Jatropha Curcas) Leaves. Are entire orbicular-ovate, angular or

somewhat 3 to 5 lobed, 10 to 18 centimeters long, acuminate with a cordate base.

(www.stuartxchange.org)

Extract. A substance made by extracting a part of a raw material, often by using a

solvent such as ethanol or water. (Merriam Webster Dictionary)

Toilet Cleanser. A chemical solutions used to disinfect or clean the toilet.

(www.definitions.net)

PROCEDURES

Production of Tubang Aso Leaves Extract. The researcher were collected

Tubang Aso Leaves in the backyard. To make sure that the leaves are clean, it was

washed carefully and placed at an open area in preparing for drying. After that, the

researcher boiled 1 liter of distilled water in preparation for extraction. For the extraction,

the researcher used the maceration method which involved soaking of the plant in the

stoppered container with a solvent like water and allowed to stand at room temperature

for at least 3-5 days. After 3-5 days, the mixture is pressed or strain by filtration to get

the Tubang Aso Leaf Extract and placed it in the container.

Antimicrobial Activity test. The amount yield of Tubang Aso leaves extract was

measured in terms of milliliters. Three (3) sets was made; agar with 50 milliliters of

tubang aso leaf extract, agar with 50 milliliters of commercial toilet cleanser and agar

without tubang aso leaf extract and commercial toilet cleanser. The production of agar

was made by having tubang aso leaf extract that was mixed carefully into the plain agar

and distilled water in a pot, the same with commercial toilet cleanser. The mixture was
heated until it become sticky. Then the agar was transfer to the test tubes in preparation

for antimicrobial activity test. With the use of cotton buds, it was used to transfer

microbes from bowl to the agar plates by the help of swabbing. It had undergone a week

of observation of control microbes. To get the final result of the research project, it had

undergone by two (2) repeated experimentation.

Production of toilet cleanser. Toilet cleanser was made by having mixture of

50 ml of tubang aso leaf extract, ½ cup of white vinegar, 5 ml of lemon juice, and ½ cup

of baking soda. With these natural ingredients, it is used to formulate alternative toilet

cleanser. Cleaning property was tested by putting toilet cleanser to the bowl and will be

observed.

Testing the effectiveness of Tubang Aso Leaves Extract. Comparative

analysis was done by using Tubang Aso leaf extract as toilet cleanser and commercial

toilet cleanser in terms of Antimicrobial property, cleansing property, odor, cost, and

expiration.
APPENDICES
 GATHERING WASHING DRYING
BGBGG

EXTRACTION
Measuring the yield in mL

CUTTING

BOILING
PLACING ADDING

STRAINING
ANTIMICROBIAL ACTIVITY TEST

MIXING TRANSFERRING SWABBING

HEATING SOLIDIFYING OBSERVATION

MIXING
TOOLS

Cotton Buds Casserole Test tube

Strainer Tubang aso leaves

 PRESENTATION OF RESULT

INPUT PROCESS OUTPUT

Equipment and Process/Steps

Mixtures used in done through:
the Investigatory • Gathering
Project: of Tubang
• Cotton Buds Aso leaves
• Casserole • Washing Tubang Aso
• Test Tube • Drying Leaves Extract as
• Strainer • Cutting an Alternative
• Tubang Aso • Boiling Toilet Cleanser
leaves • Mixing
• Distilled Water •Transferrin
• Commercial g
Toilet Cleanser • Heating
• Agar •
Observation

The presentation of this study can be seen through the framework above. This study

followed the Input which consist of the equipment and mixtures used in the Investigatory

Project. Next is the Process which presents the process/steps in making the product and

lastly, the main Output which is Tubang Aso Leaves Extract as an Alternative Toilet

Cleanser.
 Results and Discussion

In this chapter, data are shown in table form. This data are the descriptions of

Tubang Aso Leaf extract as an alternative toilet bowl cleanser. The physical aspect of

the reaction of bacteria from the research study which is Tubang Aso Leaf Extract as

Toilet Bowl Cleanser

This also presents all the data gathered from significant difference between Tubang

Aso Leaf Extract Toilet Cleanser and Commercial Toilet Cleanser in terms of

antimicrobial property, cleansing property, odor, cost and length of effectivity.

Table 1. Measuring the antimicrobial Property

TRIAL1 TRIAL 2

Agar with 50 ml of Without growing

Without growing
Tubang Aso Leaf Extract microorganisms
microorganisms
Agar with 50 ml of

Commercial Toilet Without growing Without growing

Cleanser microorganisms microorganisms

Plain agar Without growing Without growing

microorganisms microorganisms

Antimicrobial property was tested by having an antimicrobial activity test. It was

taken one (1) weeks of observation of control microbes. It had undergone by two (2)

repeated experimentation. On the first trial of the test about antimicrobial property, there

were no microorganisms that grow on the test tube of plain agar and the same with the

agar with tubang aso leaf extract and commercial toilet. On the second trial, all test tube
 with an agar was failed to have a growing microorganism. It was supposed to have a

growing microorganism on plain agar only

Table 2. Cleansing property

Cleansing property

Toilet cleanser (Tubang

Not clean
aso leaf extract)
Commercial toilet cleanser Clean

Cleaning property was tested by putting dirt to the bowl and was observed in

terms of putting toilet cleanser with Tubang Aso Leaf Extract in a left side of the bowl

and commercial toilet cleanser into the other side of the bowl. Table 2 shows that the

Toilet Cleanser (tubang aso leaf exract) were not clean and clean for commercial toilet

cleanser.

Table 3. Comparative odor of the toilet cleanser

Odor

3rd
st
1 person 2nd person 4th person 5th person
person

Toilet cleanser

(Tubang aso leaf

Like Dislike Dislike Like Dislike
extract)

Commercial toilet
Like Like Like Like Like
cleanser
 The toilet cleanser with Tubang Aso Leaf Extract and Commercial Toilet

Cleanser was done by comparing and have five (5) respondents to judge the odor of a

product. One is Toilet cleanser with Tubang aso leaf extract and one is the Commercial

toilet cleanser. In toilet cleanser with Tubang Aso Leaf Extract two (2) of respondents

were said they liked the product and three were disliked the product. But in commercial

toilet cleanser five (5) of the respondents were said that they liked the product.

Table 4. Comparative cost of toilet cleanser

Cost / amount in peso (Php)

Toilet cleanser (Tubang aso leaf extract)

30 Php

Commercial toilet cleanser 20 Php

Cost was calculated by computing all the expenses used in producing Toilet

Cleanser. Then it was compared to the cost of Commercial Toilet Cleanser.The

tabulated data shows that the cost/amount in peso of Tubang Aso Leaf Extract Toilet

Cleanser was expensive compared to the Commercial Toilet Cleanser. Cost was

calculated by computing all the expenses in Baking soda, Vinegar, lemon, distilled water

and Tubang Aso Leaf Extract that used in producing toilet cleanser.

Table 5. Comparative analysis on the expiration of the toilet cleanser

 Length of effectivity (days/weeks)

Toilet cleanser (Tubang aso leaf extract) 0 day/s

Commercial toilet cleanser 0 day/s

Length of effectivity was tested on how long the toilet cleanser can last and was

observed in Antimicrobial activity test in terms of recording the days/weeks of the control

microbes.
SUMMARY, CONCLUSION, AND RECOMMENDATION

This chapter presents the summary, conclusion and recommendations of the study

based on the data analyzed in the previous chapter.

SUMMARY

The focus of this study is to determine the effectiveness of Tubang Aso Leaves

Extract as Toilet Cleanser. This presents the significant difference between Tubang Aso

Leaf Extract Toilet Cleanser and Commercial Toilet Cleanser. It was tested in terms of

having an antimicrobial activity test in which it has undergone one (1) week of

observation and two (2) repeated experimentation, observation of its cleansing property,

computed all the expenses in making toilet cleanser, the odor, and its length of

effectivity.

Within two (2) trials of experimentation, there were no growing microorganism in the

surface of agar in test tube that is why the researchers concluded that it has no

antimicrobial property, cleansing property and length of effectivity. In terms of odor,

among the five (5) respondents, two (2) of them Liked the odor and three of them were

not. In the cost, Tubang Aso Leaf Extract Toilet Cleanser is costly than Commercial

Toilet Cleanser.

CONCLUSION

In the light of the findings, the following conclusions are hereby established:

1. The Tubang Aso Leaf Extract can’t be an effective antimicrobial solution because

according to the data gathered, there were no growing microorganisms in the

agar. The researcher concluded that the main factor why it was failed is because

of temperature and lack of resources.

 2. The Tubang Aso Leaves Extract doesn’t have an antimicrobial property because

of no growing microorganisms based on observations.

3. The effectiveness of Tubang Aso leaves extract affected and deemed

unsuccessful due to changing temperature, lack of resource and it was supposed

to place in an incubator to have a better result, have some unknown bacteria and

the insufficiencies of trials and observation.

4. Tubang Aso Leaves Extract don’t have enough cleansing property because it is

not sticky and without an antimicrobial property

5. The Tubang Aso Leaves Extract will not be acceptable for consumer because

Tubang Aso Leaves Extract Toilet Cleanser is costly compared to the

Commercial Toilet Cleanser.

After analyzing all the data and information, the proponent therefore conclude that

the Tubang Aso Leaf Extract couldn’t be an Antimicrobial Toilet Cleanser.

RECOMMENDATION

For the improvement of this investigatory project, the proponent would like to

recommend the following:

1. Further studies and information to conduct an alternative toilet cleanser

especially using Tubang Aso Leaves Extract.

2. Have patient and manage the time given in conducting the research project.

3. Make sure to have all the important materials and follow all the procedures and

methods from chapter 3.

4. Check all the needed task like temperature, place and materials.

5. Create a plan on how to make a toilet cleanser especially in testing on having an

antimicrobial activity test.

6. In conducting an investigatory project, it must show the real result of the given

experiment.
REFERENCES:

Afzal M, Kazmi I, Khan R, Singh R, Chauhan M, Bisht T, et al (2012). Bryophyllum

pinnatum: a review. Int J Res Biol Sci 2012; 2(4): 143-9.

Agnieszka Najda, A. F. (2013). Chemical composition and nutritional value of jatropha

Jatropha curcas L. leaves . Journal of Genetic and Environmental Resources

Conservation, 221-223.

Akinpelu, D.A.; Aiyegoro, O.A. and Okoh, A.I. (2009). The bioac ve poten als of two

medicinal plants commonly used as folklore remedies among some tribes in west

Africa. African J. Biotechnol., 8(8): 1660-1664.

Beni Setha, A. L. (2014). Antibacterial Activity Of Leaves Extracts Of Jatropha Curcas,

Linn Against Enterobacter Aerogenes . 129.

Chauhan, N., Kumar, P., Mishra, S., Verma, S., Malik, A., and Sharma, S. (2015).

Insecticidal activity of Jatropha curcas extracts against housefly, Musca

domestica.Environ.Sci.Pollut.Res.22,14793–14800.doi:10.1007/s11356-

0154686-1
 BIOGRAPHICAL STATEMENT

Von Rich Mancion Generoso was born on the 28 th day of December year 2003 in

Unisan, Quezon. He finished his primary education at Almacen Elementary School

during the year 2016. He accomplished his Junior High education at Unisan Integrated

High School (UIHS) formerly known as Unisan National High School (UNHS). He is

currently a Grade 12 Senior High School Student of Dominican Academy Unisan,

Quezon taking up General Academic Strand (GAS). He attended seminar and workshop

with the theme, “5H; Hand, Head, Heart. Health, and Honor for Agricultural affairs during

the year 2018. At present and during this time of pandemic, he engage himself in online

classes as the mode of new normal education. He aspire to become a Professional

Teacher someday.