Scribd
Upload
378 views8 pages

Perhitungan Tonisitas 1. Metode Ekivalensi HCL

The document describes 5 methods to calculate the tonicity of a solution: equivalence to HCl method, cryoscopic method, White Vincent method, Sprowls method, and dissociation factor method. Across the 5 methods, the solution was found to be hypotonic, requiring the addition of NaCl to achieve isotonicity with 0.9% NaCl. The osmolarity was also calculated and found to be 123.8819 mOsmol/L, indicating the solution is hypotonic.

Uploaded by

Familia Maya Sarii
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as ODT, PDF, TXT or read online on Scribd
378 views8 pages

Perhitungan Tonisitas 1. Metode Ekivalensi HCL

The document describes 5 methods to calculate the tonicity of a solution: equivalence to HCl method, cryoscopic method, White Vincent method, Sprowls method, and dissociation factor method. Across the 5 methods, the solution was found to be hypotonic, requiring the addition of NaCl to achieve isotonicity with 0.9% NaCl. The osmolarity was also calculated and found to be 123.8819 mOsmol/L, indicating the solution is hypotonic.

Uploaded by

Familia Maya Sarii
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as ODT, PDF, TXT or read online on Scribd
You are on page 1/ 8

Perhitungan Tonisitas

1. Metode Ekivalensi HCl

• Lidocaine

Liso = 3,4

E = 17 x Liso/BM = 0,247

Kesetaraan NaCl = E x Gram zam = 0,247 x 0,024 = 0,0059

• Epinephrine Bitartrate

Liso = 3,6

E = 17 x Liso/BM = 0,334

Kesetaraan NaCl = E x Gram zam 0,334 x 0,0000024= 0,0000008

• Potassium Metabisulfite

Liso = 1,9

E = 17 x Liso/BM = 0,172

Kesetaraan NaCl = E x Gram zam = 0,172 x 0,000144= 0,00002477

• EDTA Disodium

Liso = 4,8

E = 17 x Liso/BM = 0,243

Kesetaraan NaCl = E x Gram zam = 0,243 x 0,000003 = 0,00000073

• Sodium Chloride

Liso = 3,4

E = 17 x Liso/BM = 0,989

Kesetaraan NaCl = E x Gram zam = 0,989 x 0,000492 = 0,000486

• HP-B-CD

Liso = 1,9

E = 17 x Liso/BM = 0,028

Kesetaraan NaCl = E x Gram zam = 0,028 x 0,0137 = 0,00038


• Sodium Sacharine

Liso = 1,9

E = 17 x Liso/BM = 0,157

Kesetaraan NaCl = E x Gram zam = 0,157 x 0,000108 = 0,0000148

Total Tonisitas : 0,0068 gr / 100 ml

NaCl 0,9% dalam 100 ml setara = 0,9/100 x 100 ml =0,9 ml

Selisih Tonisitas = (0,9-0,0068) gram = 0,8932 gram

Hipotonis, setara kekurangan NaCl 0,9% = 0,8932 gram

2. Metode Krioskopik

Tf = Liso x g x 1000 / BM x V

• Lidocaine

(3,4 x 0,024 x 1000) / 234,34 x 100 = 0,0035

• Epinephrine Bitartrate

(3,6 x 0,0000024 x 1000) / 183,204 x 100 = 0,00000047

• Potassium Metabisulfite

(1,9 x 0,000144 x 1000) / 188,177 x 100 = 0,00001454

• EDTA Disodium

(4,8 x 0,000003 x 1000) / 336,16 x 100 = 0,00000043

• Sodium Chloride

(3,4 x 0,000492 x 1000) / 58,44 x 100 = 0,00029

• HP-B-CD

(1,9 x 0,0137 x 1000) / 1135 x 100 = 0,00023

• Sodium Sacharine

(1,9 x 0,000108 x 1000) / 205,17 x 100 = 0,00001

Total = 0,004
Selisih Penurunan Tf = 0,52-0,004 = 0,496

Setara dengan NaCl = 0,92/0,496 = 0,9/x = 0,485 gram

Hipotonis, setara kekurangan NCl 0,9% adalah 0,485 gram

3. Metode White Vincent

Vol. air = (g x E) / 0,9%

• Lidocaine

(0,247 x 0,024 ) / 0,9% = 0,65556

• Epinephrine Bitartrate

(0,324 x 0,0000624 ) / 0,9% = 0,000089

• Potassium Metabisulfite

(0,172 x 0,000144 ) / 0,9% = 0,00275

• EDTA Disodium

(0,243 x 0,000003 ) / 0,9% = 0,000081

• Sodium Chloride

(0,989 x 0,000492 ) / 0,9% = 0,054

• HP-B-CD

(0,028 x 0,0137 ) / 0,9% = 0,4222

• Sodium Sacharine

(0,137 x 0,000108 ) / 0,9% = 0,00164

Total = 0,47779 ml

100 ml - 0,47779 ml = 99,5221 ml

0,9/x = 100/99,5221 = 0,8957 gram

Hipotonis, setara kekurangan 0,8957 gram NaCl

4. Metode Sprowls
V1 = (Liso x g x 1000) / (BM x 0,52)

V2 = (V1 x g) / 0,3

• Lidocaine

(3,4 x 0,024 x 1000) / (234,34 x 0,52) = 0,669 X 0,024 / 0,3 = 0,535

• Epinephrine Bitartrate

(3,6 x 0,0000024 x 1000) / (183,204 x 0,52) = 0,00000001 X 0,0000024 / 0,3 =


0,00000000000008

• Potassium Metabisulfite

(1,9 x 0,000144 x 1000) / (188,177 x 0,52) = 0,00000015 X 0,000144 / 0,3 =


0,000000000072

• EDTA Disodium

(4,8 x 0,000003 x 1000) / (336,16 x 0,52) = 0,00000002 X 0,000003 / 0,3 =


0,0000000000002

• Sodium Chloride

(3,4 x 0,000492 x 1000) / (58,44 x 0,52) = 0,00000955 X 0,000492 / 0,3 =


0,00000002

• HP-B-CD

(1,9 x 0,0137 x 1000) / (1135 x 0,52) = 0,00000039 X 0,0137 / 0,3 = 0,00000002

• Sodium Sacharine

(1,9 x 0,000108 x 1000) / (205,17 x 0,52) = 0,00000009 X 0,000108 / 0,3 =


0,0000000000324

Jumlah yang ditempati zat = 0,053826

Volume berlebihan = 100-0,053826 = 99,94617

Kelebihan volume setara dengan = 0,9/x = 100/99,94817 = 0,8995 gram

5. Metode Faktor Disosiasi

• Lidocaine

Konsentrasi (g/L) = 0,1


Fa = 1,5

Tonisitas zat = (Fa/BM) x c = 140,604

• Epinephrine Bitartrate

Konsentrasi (g/L) = 0,00004

Fa = 1,8

Tonisitas zat = (Fa/BM) x c = 0,01319

• Potassium Metabisulfite

Konsentrasi (g/L) = 0,0024

Fa = 1,5

Tonisitas zat = (Fa/BM) x c = 0,6774

• EDTA Disodium

Konsentrasi (g/L) = 0,0005

Fa = 1,5

Tonisitas zat = (Fa/BM) x c = 0,2521

• Sodium Chloride

Konsentrasi (g/L) = 0,082

Fa = 1,8

Tonisitas zat = (Fa/BM) x c = 8,6257

• HP-B-CD

Konsentrasi (g/L) = 0,02277

Fa = 1,8

Tonisitas zat = (Fa/BM) x c = 46,5191

• Sodium Sacharine

Konsentrasi (g/L) = 0,0018

Fa = 1,8

Tonisitas zat = (Fa/BM) x c = 0,6647


Total = 197,35619

Selisih Tonisitas = 3,2 x 0,28/197,35619 = 0,00454 gram/ 1000 ml

Massa NaCl berlebih dalam 100 ml = 0,00454/1000 x 100 = 0,000454 gram

PERHITUNGAN OSMOLARITAS

• Lidocaine

= m zat/BM x n x 1000

= 24 / 234,34 x 1 x 1000

= 102,45 mOsmol/L

• Epinephrine Bitartrate

= m zat/BM x n x 1000

= 0,0024 / 183,304 x 1 x 1000

= 0,013 mOsmol/L

• Potassium Metabisulfite

= m zat/BM x n x 1000

= 0,144 / 188,177 x 1 x 1000

= 0,428 mOsmol/L

• EDTA Disodium

= m zat/BM x n x 1000

= 0,003 / 336,16 x 1 x 1000

= 0,0089 mOsmol/L

• Sodium Chloride

= m zat/BM x n x 1000

= 0,492 / 58,44 x 1 x 1000

= 8,419 mOsmol/L

• HP-B-CD
= m zat/BM x n x 1000

= 13,662 / 1135 x 1 x 1000

= 12,037 mOsmol/L

• Sodium Sacharine

= m zat/BM x n x 1000

= 0,108 / 205,17 x 1 x 1000

= 0,526 mOsmol/L

Total Osmolaritas = 123,8819

Nilai Osmolaritas yang diperoleh berada dalam rentang <350 yang menandakan larutan bersifat
hipotonis, sehingga diperlukan penambahan HCl

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy