St. Alexius College, Inc.: Activity No. 2 Grossing

ST. ALEXIUS COLLEGE, INC.
ACTIVITY NO. 2
GROSSING
I. INTRODUCTION:
Grossing, often referred to as “cut-up” may follow fixation. This involves a careful examination
and description of the specimen that will include the appearance, the number of pieces and their
dimensions. The specimens may require further dissection to select appropriate areas for examination.
Specimens that are to be processed will be placed in suitable labeled cassettes (small perforated baskets)
to segregate them from other specimens.
The duration of the processing schedule used to process the specimens will depend on the type
and dimensions of the largest and smallest specimens, the particular processor employed, the solvents
chosen, the solvent temperatures and other factors.
II. OBJECTIVES:
At the end of this laboratory exercise, the student is expected to:
1) discuss the grossing technique as one of the steps in routine tissue processing;
2) perform skillfully the steps of grossing;
3) enumerate the sources of error in performing the procedure; and
4) discuss the importance of performing this method in routine tissue processing.
III. MATERIALS:
Cutting board (plastic)
Knife
Fixed tissue specimen
Tissue cassette
Page | 9
IV. PROCEDURE:
1. Wear a laboratory gown.
2. Wash hands and wear apron, mask, gloves, goggles and head cap.
3. Remove the specimen from the 10% formalin container using the forceps. Make sure to drain
excess solution from the specimen into the container.
4. Place the specimen on a clean plastic cutting board and carefully examine the specimen.
5. Cut the specimen to prepare a tissue size of 2cm2 and no more than 4mm-thick using a clean
knife. Prepare at least 10 slices. Make sure to obtain slices from appropriate areas of the
specimen.
6. Place each slice of tissue inside a clean tissue cassette.
7. Label each tissue cassette with an identifier (Example: Three tissue slices will be coded as
SP14-50001-A1, SP14-50001-A2 and SP14-50001-A3, respectively).
● SP14-50001: Accession Number

● A-1: Specimen number and Block number
8. Record the number of tissue cassettes prepared in the specimen processing report sheet.
9. Dispose used and contaminated materials on appropriate waste container.
Page | 10
REPORT SHEET
ACTIVITY NO. 2
GROSSING
NAME: ___________________________________________ RATING: ___________
DATE PERFORMED: __________________ DATE SUBMITTED: ______________
ILLUSTRATIONS:
Draw and label the procedure of grossing.
Page | 11
REVIEW QUESTIONS:
1. Enumerate at least five (5) problems encountered in grossing.
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
_____________.
2. Discuss the importance of grossing in routine tissue processing.
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
______________.
3. What are the areas of the tissue that need to be subjected for grossing?
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
__________________________________________________________________________.
Page | 12
ACTIVITY NO. 2
GROSSING
Performance Assessment Sheet
Name:_________________________________________ Date:_________________________
PROCEDURE
You must: RATING REMARKS
1. Wear a laboratory gown.
2. Wash hands and wear apron, mask,

gloves, goggles and head cap.
Grossing:
3. Remove the specimen from the 10%
formalin container using the forceps.
Make sure to drain excess solution from
the specimen into the container.
4. Place the specimen on a clean plastic

cutting board and carefully examine the
specimen.
5. Cut the specimen to prepare a tissue

size of 2cm2 and no more than
4mm-thick using a clean knife. Prepare
at least 10 slices. Make sure to obtain
slices from appropriate areas of the
specimen.
6. Place each slice of tissue inside a clean

tissue cassette using the forceps.
7. Label each tissue cassette with an

identifier (Example: Three tissue slices
will be coded as SP14-50001-A1,
SP14-50001-A2 and SP14-50001-A3,
respectively).
Page | 13
● SP14-50001: Accession number

● A-1: Specimen number and Block
number
8. Record the number of tissue cassettes
prepared in the specimen processing
report sheet.
9. Dispose used and contaminated
materials on appropriate waste
container.
RATING:
4 – EXCELLENT
3 – VERY SATISFACTORY
2 – SATISFACTORY
1 – POOR
0 – NOT DONE
LEVEL OF COMPETENCY:
Evaluator: Evaluation Date:
Student:
Page | 14

St. Alexius College, Inc.: Activity No. 2 Grossing

Uploaded by

Copyright:

Available Formats

St. Alexius College, Inc.: Activity No. 2 Grossing

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

St. Alexius College, Inc.: Activity No. 2 Grossing

Uploaded by

Copyright:

Available Formats

ST. ALEXIUS COLLEGE, INC.

1. Wear a laboratory gown.

6. Place each slice of tissue inside a clean tissue cassette.

● SP14-50001: Accession Number

9. Dispose used and contaminated materials on appropriate waste container.

NAME: _________________________________ RATING: _

DATE PERFORMED: ____ DATE SUBMITTED:

Draw and label the procedure of grossing.

1. Enumerate at least five (5) problems encountered in grossing.

2. Discuss the importance of grossing in routine tissue processing.

2. Wash hands and wear apron, mask,

4. Place the specimen on a clean plastic

5. Cut the specimen to prepare a tissue

6. Place each slice of tissue inside a clean

7. Label each tissue cassette with an

● SP14-50001: Accession number

Evaluator: Evaluation Date:

You might also like

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.