Alya Lab Report

FACULTY OF PLANTATION AND AGROTECHNOLOGY
DIPLOMA IN HERBAL PRODUCTION

UiTM BRANCH SARAWAK CAMPUS MUKAH
BOTANY AND TAXONOMY OF PLANTS (AGR149)
LABORATORY REPORT 1
HANDLING MICROSCOPE
NAME: ALYA NISA BINTI BADRUL HISHAM
CLASS: AT112A
STUDENT ID: 2022841114
LECTURER: MS. NURUL SYAHIRAH MANSUR
For LECTURER use only
DATE RECEIVED :
STAMP :
1
1.0 Introduction
A contemporary microscope was first created by Anthony Van. Microscope is the
most powerful tool especially in the microbiology labatory which gives an
enlargement which allow us see the structure invisible microorganism by naked
eye.The human eye can distinguish object about 0.1mm apart. Every student that
learn microbiology must known what is microscope and understanding the basic of
components tool of a microscope because student biologist have to study objects
much smaller like bacteria , plants and animal cell.The microscope has the working
principle is by reflecting light passes through the mirror then continues to the
objective lens .In the shadow objective produced is virtual, inverted,and
differentiated.
2 type that laboratory provides are light microscope and disseting microscope
.The light microscope were the first and still common type of microscope.Light is
from a light source through specimen , the image of which is magnified by glass
lens .The light microscope is easy to use and suitable for students for student living
cells. Then ,light microscope shows a 2D image . After that ,the disseting microscope
there also known as stereoscopic microscope . The reflect effect light from the
surface of a specimen . The disseting microscope shows a 3D image . Use to
viewing parts of a specimen or entire organism. It use to magnified object that not
transparent and use to magnified object that to large.
2.0 Objectives
The objective/s for this laboratory report is/are:
i. To label and give the function of basic part of the light microscope.
ii. To list in proper order the step involved in microsope handling and
maintenance.
iii. To calculate the diameter of the field view and the total magnifation for both
low and high power lens system.
3.0 Materials and Apparatus
Materials: Light microsope , slide and cover slip ,scissors, filter paper and tissue
paper.
Apparatus: Old newspaper, distilled water and staining (metil blue).
2
4.0 Procedure
A.PROCEDURE OF MICROSCOPE HANDLING
1. The microscope was put on the table.

2. Socket was plug to the power source
3. The light was switch. Controlled the light controller to get the appropriate light
brightness
4. The condenser aperture diaphragm was fully open.
5. Pull the slide holder when the letter k was place at the mechanical stage.
6. The letter k position was exactly centered in the field and the specimen focus
was under low power.
7. The lowest powered objective (4x) in positionwas placed.
8. Used the course adjustment to moved the stage up until the letter came into
focus.
9. Fine adjustment was adjusted to get the sharp focus.
10. When looking through the eyepieces had practice eyes open to reduces
eyestrain.
11. The magnification of the specimen was describe.
12. After finish, the microscope was return to the lowest power objective and the
slide was remove from the stage.
B. SLIDE PREPARATION (LETTER ‘K’ SLIDE) AND STAINING
1. Dropped of water on the middle of a slide.

2. The small letter of k was cute from newspaper and wait for a few seconds
after place water on top of it.
3. Coverslip on the slice was placed on edge and to prevent the formation of air
bubble carefully lower over the drop so it can be done with filter paper.
4. No more bubble was trapped when practice this technique under the slip
cover.
5. When the letter ‘k’ was ready ,drop 1-2 of staining at the edge of one end the
coverslip.
6. The other end of the slide was placed a piece of filter paper. The tissue paper
will absorb staining by capillary through the cover slip.
3
RESULTS / OBSERVATION
A.LABEL THE MICROSCOPE
OCULAR
ARMS
OBJECTIVE LENS
STAGE
STAGE CLIPS
BASE
FIGURE 1: Disseting microcope
4
OCULAR
REVOLVING
NOSEPIECE
ARM
OBJECTIVE
STAGE CLIP COURSE

FOCUS
STAGE FINE
FOCUS
CONDENSER
LIGHT BASE
SOURCE
FIGURE 2:LIGHT MICROSCOPE
B. RESULT
4x
FIGURE 3: The letter ‘k’ under the light microscope

3.Total magnification
Formula: MAGNIFICATION =OBJECTIVE POWER x EYEPIECE IMAGE
Magnification =4x X 10x
=40x
4. Field of view
Formula:FIELD OF VIEW = EYEPIECE x MAGNIFICATION OF OBJECTIVE
LENS
FOV =
5
5.0 Discussion
After conducting the experiment, when used the microscope the image and object
was different . The image under the microscope was became upside down
compared to original position on the slide. The image wat not flipped sideways but
the image was in an upside down position. The movement on the slide does match
the movement under the microscope. The microscope lens need to be well taken
care of so there is no fungus that can damage the microscope and it is necesassary
to use lens paper to avoid damage.
The method after using the microscope that I learned were to close the switch
every time it is not in use and after all the experiments have been completed, it is
necessary to close the switch first and then close the plug and pull the plug carefully.
After that, put the microscope in the original place provided in the laboratory. The
way to lift it is to use both hands, one hand is on the base and the other hand is on
the arm of the microscope to prevent the microscope from falling.Then cover the
microscope with the caps or plastic that laboratory provide.Never allow the slide
touch the lens because dirty lenses can be difficult to clean.
6.0 Conclusion
7.0 References
Follow the format of reference lists using APA style. Make sure citation in reference
list is present in in the text citation in the paragraph. Example of reference in text
citation and reference list is given below.
Chia Chay Tay, Najihah Mohamad Nasir, Nur Liyana Jamaluddin and Wan Nurul
Hidayah Wan Annuar. Pertanika J. Sci. & Technol. 27(2): 619 - 632 (2019)
Sustainable Approach using Carica papaya Stem for in vitro Propagation of
Clinacanthus nutans
In text citation: ……………….. (Tay et al. 2019).
In list of references:
6
1. Tay, C.C., Najihah, M.N., Nur Liyana, J. and Wan Nurul Hidayah, W.A. 2019.
Sustainable approach using Carica papaya stem for in vitro propagation of
Clinacanthus nutans. Pertanika J. Sci. & Technol. 27(2): 619-632

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FACULTY OF PLANTATION AND AGROTECHNOLOGY

DIPLOMA IN HERBAL PRODUCTION

BOTANY AND TAXONOMY OF PLANTS (AGR149)

NAME: ALYA NISA BINTI BADRUL HISHAM

STUDENT ID: 2022841114

LECTURER: MS. NURUL SYAHIRAH MANSUR

For LECTURER use only

The objective/s for this laboratory report is/are:

3.0 Materials and Apparatus

A.PROCEDURE OF MICROSCOPE HANDLING

1. The microscope was put on the table.

B. SLIDE PREPARATION (LETTER ‘K’ SLIDE) AND STAINING

1. Dropped of water on the middle of a slide.

A.LABEL THE MICROSCOPE

FIGURE 1: Disseting microcope

STAGE CLIP COURSE

FIGURE 2:LIGHT MICROSCOPE

FIGURE 3: The letter ‘k’ under the light microscope

In text citation: ……………….. (Tay et al. 2019).

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