Cambridge International AS & A Level: Biology 9700/13
Cambridge International AS & A Level: Biology 9700/13
Cambridge International AS & A Level: Biology 9700/13
BIOLOGY 9700/13
Paper 1 Multiple Choice October/November 2022
1 hour 15 minutes
INSTRUCTIONS
• There are forty questions on this paper. Answer all questions.
• For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
• Follow the instructions on the multiple choice answer sheet.
• Write in soft pencil.
• Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
• Do not use correction fluid.
• Do not write on any bar codes.
• You may use a calculator.
INFORMATION
• The total mark for this paper is 40.
• Each correct answer will score one mark.
• Any rough working should be done on this question paper.
IB22 11_9700_13/5RP
© UCLES 2022 [Turn over
2
If the actual length of this chloroplast measured along X–Y is 10 µm, what is the magnification of
the image?
1 endoplasmic reticulum
2 Golgi body
3 mitochondrion
4 chloroplast
A 1, 2, 3 and 4
B 1, 2 and 4 only
C 1 and 2 only
D 3 and 4 only
cytoplasm
C
B
mitochondrion nucleus
D
prokaryotic
feature mitochondria
cells
6 The Benedict’s test and ethanol emulsion test were carried out on a sample of biological
molecules. The solution became brick-red during the Benedict’s test and cloudy during the
ethanol emulsion test.
A B
CH2OH OH
C O C O
H H H H
H H
C C C C
OH H OH H
HO OH HO OH
C C C C
H OH CH2OH OH
C D
OH CH2OH
C O C O
H OH H OH
H H
C C C C
OH H OH H
HO H HO H
C C C C
CH2OH OH H OH
9 The diagram represents part of an alginic acid molecule. Alginic acid is a polymer found in the
cell walls of brown seaweeds.
COOH H H
O
H O H
H OH HO
OH HO COOH
O
H H
O
H H H n
A Alginic acid is a linear polysaccharide containing 1,6 glycosidic bonds between each hexose
sugar.
B Alginic acid is formed from two monomers joined by 1,4 glycosidic bonds.
C Alternate monosaccharides are arranged in opposite directions; this is also seen in glycogen
molecules.
D Hexose sugars and water molecules are joined by condensation reactions to make alginic
acid.
A 1, 2, 3 and 4
B 1 and 3 only
C 2, 3 and 4 only
D 2 and 4 only
11 A scientist studying the structure of a protein reported that it consists of two polypeptide chains
joined by disulfide bonds.
A primary structure
B secondary structure
C tertiary structure
D quaternary structure
12 Some molecules fluoresce when illuminated with ultraviolet light. Changes in the structure or
shape of such molecules can change the intensity of the fluorescence.
Adding serine to the enzyme increases the intensity of the fluorescence, but when indole is also
added the bluish-green fluorescence decreases in intensity.
13 Yeast contains the enzyme catalase which catalyses the breakdown of hydrogen peroxide (H2O2)
as shown.
catalase
2H2O2 2H2O + O2
Yeast was added to a solution of hydrogen peroxide and the total volume of oxygen released was
recorded every 30 seconds for 2 minutes. All other variables were standardised.
total volume of
time / s
O2 / cm3
30 157
60 251
90 283
120 285
14 The Michaelis–Menten constant, Km, is a measure of the affinity of an enzyme for its substrate.
15 Which features are correct for active transport and facilitated diffusion?
16 The diagram represents two solutions, P and Q, that were separated by a partially permeable
dialysis tubing.
partially permeable
dialysis tubing
What will be the initial movement of the molecules, 1, 2 and 3, between solution P and
solution Q?
17 A student weighed a cylinder of potato and then put it into a test-tube containing a salt solution.
The potato cylinder was removed from the salt solution after one hour. It was blotted dry and then
reweighed. The student recorded that the potato had lost mass.
Which row shows the correct explanation for the results the student collected?
A higher plasmolysed
B higher turgid
C lower plasmolysed
D lower turgid
18 Which row shows the correct number of each component in a single chromosome at the start of
telophase of mitosis?
polynucleotide
centromeres telomeres
strands
A 1 2 2
B 1 4 4
C 2 4 2
D 2 2 4
A B C D
20 The diagram shows the epidermis of human skin. This is formed of four layers of cells.
Cells in each layer have specific functions which are outlined on the diagram. As the cells are
produced, they move upwards as shown by the arrow.
Which row shows cells that are acting as stem cells in the skin epidermis and an organelle
needed by the keratinocytes?
21 The diagram shows some of the stages that take place during the cell cycle.
1
number of DNA
molecules in
cell doubles
4 stages
2
nuclear envelope during cell
cell grows
disappears cycle
3
chromosomes
become visible
1 an amino acid
2 a codon
3 a nitrogenous base
4 a sugar
23 A diagram can be used to show some relationships between different nucleic acid bases.
1 2 3 4 5
Which row is correct for the words that can be placed at positions 1–5?
1 2 3 4 5
Some of the bacteria were moved from a heavy nitrogen medium and cultured in a medium with
only light nitrogen, 14N (0 minutes).
These bacteria continued to reproduce and samples were extracted and centrifuged at regular
intervals.
The diagram shows the possible positions (upper, middle and lower) of the bands of DNA. The
actual positions of bands in the first two samples are shown.
upper upper
middle middle
lower lower
0 50 100 150
sample time:
minutes minutes minutes minutes
Which proportion of the DNA of the sample taken at 150 minutes will be at the upper position?
How many nucleotides does the mRNA coding for α-globin contain?
28 Which xerophytic adaptations reduce the water potential gradient between leaf surface and
atmosphere?
1 rolled leaves
2 hairy leaves
3 sunken stomata
4 fewer stomata
5 fleshy leaves
A 1, 2, 3, 4 and 5
B 1, 2 and 3 only
C 1, 3 and 4 only
D 2 and 5 only
1 2 3 4 5 6 7
Which row identifies the correct stages in which the tulip bulb is acting as a source only or as a
sink only?
source sink
only only
A 1 5
B 3 4
C 4 6
D 5 7
30 What would be changed if mitochondrial activity was inhibited by a metabolic poison acting on
cells in the phloem tissue?
31 By which process does sucrose move through phloem sieve tube elements?
A active transport
B diffusion
C facilitated diffusion
D mass flow
1 coronary artery
2 vena cava
3 pulmonary artery
4 pulmonary vein
34 At one point in the cardiac cycle, the pressure in the right ventricle is lower than that in the right
atrium and lower than that in the pulmonary artery.
atrioventricular
semilunar valve
valve
A closed closed
B closed open
C open closed
D open open
36 The graph shows the dissociation curves for haemoglobin at two different partial pressures of
carbon dioxide.
At which position on the graph would most chloride ions be found in the red blood cells?
percentage saturation of
haemoglobin with oxygen high carbon dioxide
partial pressure
A
B
What would increase the amount of tissue fluid around body cells?
38 Certain bacteria develop resistance to an antibiotic by actively transporting the antibiotic out of
the cell. This prevents the antibiotic building up to toxic concentrations.
What must be found in a bacterial cell to allow it to develop this form of antibiotic resistance?
A
B C
source of
chemical energy
39 Which statements explain the differences between a primary and a secondary response to
exposure to the same antigen?
1 The primary response is slow, because there are only a few B-lymphocytes that are
specific to the antigen.
2 The secondary response is faster, because there are many memory cells specific to
the antigen.
3 More antibodies are produced during the secondary response, because many of the
memory cells differentiate into plasma cells specific to the antigen.
A They become active only when a specific antibody binds to their surface receptor.
B They divide to form clones when meeting an antitoxin in a cell.
C They produce memory cells to respond to an antigen when exposed in the future.
D They release hormone-like cytokines which stimulate release of antibodies.
BLANK PAGE
BLANK PAGE
Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge
Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download
at www.cambridgeinternational.org after the live examination series.
Cambridge Assessment International Education is part of Cambridge Assessment. Cambridge Assessment is the brand name of the University of Cambridge
Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge.