QuickStart manual

VWR® Microbiological Air Sampler

SAS Super ISO USB

EU Catalogue Number:
710-2088
710-2087
710-2090
710-2089

VERSION: 2
ISSUED: APRIL 2021
 Legal address of
manufacturer

Europe
VWR International Srl
Via S. Giusto 85
20153 Milano
Italy
Tel.: + 39 0233 203 11
http://it.vwr.com

Country of origin Italy

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Table of contents
LEGAL ADDRESS OF MANUFACTURER BRIEF INSTRUCTIONS
2 5

WARNING INSTALLATION
3 6-7

SAFETY INFORMATION TROUBLESHOOTING

3 7

TECHNICAL FEATURES ACCESSORIES

4 8

INTENDED USE
4-5

A more detailed instruction manual is available

for download on our website vwr.com.

Warning!
This document is the property of VWR International S.r.l. - Milan - Italy
It may not be duplicated or distributed without the owner’s authorisation. Patent Pending
Manufactured by VWR International S.r.l. - Milan, Italy

Safety Information
Please use this device only for the purposes indicated.
The device must be correctly used according to this instructions manual before starting any
operation. Any electrical cables need to be replaced immediately when damaged; never use a
damaged or worn electrical cable.

Always disconnect the charger before:

‒‒ Repairing or maintenance; these operations must be carried out by qualified staff
‒‒ Cleaning the unit
Use original spare parts and accessories for any replacement.
Do not use this device in the presence of explosive gas.
Please follow the guidelines below and read this manual in its entirety to ensure safe
operation of the unit.
Be aware that the voltage and frequency of the electrical system are
compatible with the requirements of the battery charger.
Never use a non-OEM charger to charge the air sampler. Use of an improper
harger may damage the unit.
Carefully read this manual before operating your instrument.

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Technical features
Feature SAS SUPER ISO USB 100 Contact SAS SUPER ISO USB 180 Contact SAS SUPER ISO USB 100 Petri SAS SUPER ISO USB 180 Petri
Cat. No. 710-2088 710-2087 710-2090 710-2089
Air flow 100 l/min 180 l/min 100 l/min 180 l/min
Use with 55 mm contact plates 55 mm contact plates 90 mm Petri dishes 90 mm Petri dishes
Portable Yes Yes Yes Yes
Battery Rechargeable Rechargeable Rechargeable Rechargeable
Battery life 70 000 litres 40 000 litres 70 000 litres 40 000 litres
Speed sensor Yes Yes Yes Yes

Intended use
Principle
The Surface Air System (SAS) encompasses several models which use the same principle.
Air is aspirated at a fixed speed for a variable time through a cover which has been machined
with a series of specially designed small holes. The resulting laminar airflow is directed onto the
agar surface of a “contact plate” (or Petri dish) containing media suitable for the microbiological
examination to be performed. When the pre-set sampling cycle is completed, the plate is
removed and incubated. The organisms are then visible to the naked eye and can be counted in
order to assess the level of contamination.

The basic idea

The major points of the Surface Air System (SAS) are: 4 To accumulate data on the level of hygiene in each environment
1 To use a simple and inexpensive “contact plate” (“SURFAIR PLATE”, so that fluctuations can be monitored.
“RODAC”) for surface, hands or air control. These plates are very well 5 To take advantage of advanced electronics for more reliable
known and easily available and can be purchased ready poured with results in different operating conditions.
different media. 6 To have the flexibility to choose between 55 mm contact plates
2 To sample a known volume of air for a variable time to provide a or 90 mm standard disposable Petri dishes.
large range of sampling volumes. 7 To apply cGLP and cGMP to air sampling operations.
3 To aspirate air in a laminar flow pattern with sufficient velocity to 8 To organise sequential sampling to obtain a more representative
impact organisms on an agar surface. sample under actual operating conditions.

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Models available
SAS SUPER ISO USB 100 and SAS SUPER ISO USB 180: Two instruments for
two different applications.
The two air samplers have the same performances with the only difference being in
the rate of air aspirated:
SAS SUPER ISO USB 100 = 100 litres of air per minute.
SAS SUPER ISO USB 180 = 180 litres of air per minute.
The SAS SUPER ISO USB 180 is appropriate for use in cleanrooms and other applications
demanding fast sampling times. In cleanrooms, for example, it is important to test larger volumes
of air, because microbial air contamination is very low. The SAS SUPER ISO USB 180 reduces the
time required to obtain a sample.
Both SAS SUPER ISO USB 100 and SAS SUPER ISO USB 180 are identified as “SAS
SUPER ISO USB”.

Brief instructions
Brief operating instruction for SAS Super ISO USB 100 and 180
The airflow can be seen on a digital display at the fourth automatic succession presentation each
time the instrument is switched on.
1 Touch the OK button for at least one seconds to switch the instrument on.

2 Press OK to sample the same air volume as the last sampling cycle.
3 To change the volume of air, press or to reach “SELECT VOLUME”, then press OK and use
the arrows to choose the new desired volume of air.
4 Refer to instruction manual to change settings.
5 Press OK to confirm selection.
6 Press or , for menu selection to modify other pre-set parameters.
7 Press OK to reach the sub menu of the chosen parameter.
8 Refer to the instruction manual to change settings.
9 Press each time you need to end an action. The unit will then go back to its initial
configuration.
10 Press , and confirm by pressing OK to switch the unit off.

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Installation
The practical use of contact plates

1 Remove the aspirating head.

2 Insert an identified, closed and prepared plate and remove
the plate lid.
3 Replace the aspirating head.
4 Select required volume and start the unit. The airflow is
directed into the agar surface of the plate.
5 At the end of the cycle, remove the aspirating head.
6 Close and remove the plate.
7 Incubate.
8 Count the colonies, record the results on the
microbiological air sampling report and read the results.

List of menus and utility sub menus

OK Press to repeat the same volume of prior sampling

or Press to enter the following sub menus
Press to switch off (in the Main Screen), or each time you need to end an action.
SELECT VOLUME The volume of aspirated air can be modified according to 8 programmed values
SELECT MULTI-CYC The air sampler can be programmed (8 programmable configurations) to extend
the total sampling time using “sequential interval time” sampling.
SELECT SITE The sites of aspirating air can be selected according to a maximum of 20 programmed positions
SELECT OPERATOR The operators of aspirating air can be selected according to a maximum of 20 programmed IDs
SET DELAY The air sampler can be programmed to start sampling after a programmable
delay (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes)
OPTIONS To change general settings of the device. See description below
BATTERY Press to see the battery status
OPTIONS Press OK to change general settings of the device
EDIT VOLUMES
Volumes can be added, modified or deleted, to a maximum of 20 values
EDIT MULTI-CYC
Multiple cycles can be added, modified or deleted, to a maximum of 20 values, parameters
are INTERVAL TIME, NUMBERS OF RUN, SINGLE RUN VOLUME and TOTAL RUNS

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 EDIT SITES
Sampling sites can be added, modified or deleted, to a maximum of 20 positions
EDIT OPERATORS
Operators can be added, modified or deleted, to a maximum of 20 users
DISPLAY RECORD
To show the recorded sampling data
STORE PDF
To store the recorded sampling data in a PDF file on an USB stick
STORE XML
To store the recorded sampling data in a XML file on an USB stick
CLEAR RECORD
To clear the recorded sampling data
SET TIME
To programme date and time
SET AUTOSWITCH
To disable the auto switch off
PASSWORD ADMIN
To lock the device, still possible to start and stop a sampling procedure
PASSWORD PDF
To lock exported PDF
LOGIN
To use a user management for data integrity
LANGUAGE
To display text in different languages
STORE CONFIG
To store all configuration (Volume, Muli-Cycl, Sites, Operators…) on a USB stick
LOAD CONFIG
To load a config file of an existing Air sampler to implement all setting
(Volume, Multi-Cycle, Sites, Operators,…) on a new device

Troubleshooting
Review the information in the table below to troubleshoot operating problems.

Problem Cause Solution

Unit does not start Instrument is not turned on Touch the OK button for at least two seconds
Battery is low Recharge battery
Battery charger is not working Check battery charger and if it needs
replacing order Cat. No. 710-0993
Battery is too old Check battery and replace it
Unit does not react Malfunction or USB Error Press “C” + “OK” + “Up” simultaneously
to switch of device
Battery discharges after Battery is low Recharge battery
few minutes working
Battery charger is not working Check battery charger and if it needs
replacing order Cat. No. 710-0993
Battery is too old Check battery and replace it
“LOW BATTERY” message Battery is low Recharge battery
“CALIBRATION EXPIRED” message Instrument needs to be calibrated Send the instrument to VWR International
or an authorised dealer
MOTOR ERROR Motor not working or not connected Connect the motor
USB ERROR USB Stick detection failed Format the USB stick to the FAT32 file system
Check USB Stick and replace it
Infrared remote switch doesn’t Remote control battery is low Replace infrared remote battery
switch the unit on
Microbiological media is Media is damaged Control expiration date of media and check that
dehydrated after sampling the agar is not dehydrated before sampling

Sampling time is too long Shorten the sampling time

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Accessories
Description Cat. No.
Aspirating heads for contact plates, Ø 55 mm
Stainless steel 710-0880
Aluminium 710-0892
Sterile daily 710-0890
Aspirating heads for Petri dishes, Ø 90 mm
Stainless steel 710-0878
Aluminium 710-0886
Sterile daily head 710-0891
Other accessories
Soft carrying case 710-0896
Aluminium carrying case 710-0875
Bio-Transport autoclavable carrying case 113-8185
Handle for Bio-Transport carrying case 113-8186
Floor tripod 710-0889
SAS-Holder table and wall stainless steel 710-0963
Battery charger with universal plug for both models 710-0993
Adapter* to convert contact plate model to accept 90 mm Petri dishes 710-0882
SAS stainless steel Petri head + adaptor 710-0877
SAS aluminium Petri head + adaptor 710-0879
IQ OQ PQ validation protocols for SAS Super ISO USB 100 and 180 710-0956
Infrared remote control for SAS Super ISO USB 710-0969

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