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23 - DNA Replication Practice Worksheet 2

DNA replication is the process by which a cell makes an identical copy of its DNA before cell division. It occurs in the nucleus of eukaryotic cells and the cytoplasm of prokaryotic cells. The DNA double helix unwinds and each single strand serves as a template for new complementary strands to be synthesized according to base pairing rules, with A bonding only with T and G bonding only with C. This ensures each new cell receives an exact copy of the original DNA sequence.

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163 views2 pages

23 - DNA Replication Practice Worksheet 2

DNA replication is the process by which a cell makes an identical copy of its DNA before cell division. It occurs in the nucleus of eukaryotic cells and the cytoplasm of prokaryotic cells. The DNA double helix unwinds and each single strand serves as a template for new complementary strands to be synthesized according to base pairing rules, with A bonding only with T and G bonding only with C. This ensures each new cell receives an exact copy of the original DNA sequence.

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kandycepittma
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Name___________________________________________________Date_________________Period_______

DNA Replication Practice Worksheet

The double helix of DNA unwinds and each side serves as a pattern to make a new molecule.

Image courtesy U.S. Department of Energy Human Genome Program

DNA Replication

DNA carries the information for making all of the cell's proteins. These proteins implement all of the functions
of a living organism and determine the organism's characteristics. When the cell reproduces, it has to pass all of
this information on to the daughter cells.

Before a cell can reproduce, it must first replicate, or make a copy of, its DNA. Where DNA replication occurs
depends upon whether the cells is a prokaryote or a eukaryote (see the RNA sidebar on the previous page for
more about the types of cells). DNA replication occurs in the cytoplasm of prokaryotes and in the nucleus of
eukaryotes. Regardless of where DNA replication occurs, the basic process is the same.

DNA Replication is a process that must be done exactly. When it is not completed correctly, mutations
(mistakes) then result. In order to make sure that everything is replicated correctly, you will want to make sure
that you use the proper base pairing rules: A will bond with T only, and G will bond with C only.

Directions:
For each strand of DNA, show the three steps to demonstrate that you could “replicate” the DNA correctly. See
the example for details if you don’t recall how.

Example:
TCCTG ACCCC GCCGG GATAT CCTTC TACCT CCAAA TGTAT
Solution in two parts:
A. Fill in the complementary strand.
Original DNA: TCC TG ACCCC GCCGG GATAT CCTTC TACCT CCAAA TGTAT
Complementary: AGGAC TGGGG CGGCC CTATA GGAAG ATGGA GGTTT ACATA

B. Split the DNA and fill in the complementary strands to create two complete double helix strands.
T CCTG ACCCC GCCGG GATAT CCTTC TACCT CCAAA TGTAT (original)
AGGAC TGGGG CGGCC CTATA GGAAG ATGGA GGTTT ACATA (new)

TCCTG ACCCC GCCGG GATAT CCTTC TACCT CCAAA TGTAT (new)


AGGAC TGGGG CGGCC CTATA GGAAG ATGGA GGTTT ACATA (original) comp. from example A

1.
A. Original DNA: CCTAT ATCTC TCTAT ATCTC TCATA CTGTG TGTCT CTATA
Complementary DNA:_______________________________________________________________________

B. Make identical strands of DNA


CCTAT ATCTC TCTAT ATCTC TCATA CTGTG TGTCT CTATA (original)
________________________________________________________ (new)

_________________________________________________________ (new)
_________________________________________________________ (compl. From 1A)
2. A. Original DNA: CCGGA TTTTA ATTAG CTACT ATCGT ACTAC GTTGG TGCTA
Complementary DNA: _______________________________________________________________________

B. Make identical strands of DNA


CCGGA TTTTA ATTAG CTACT ATCGT ACTAC GTTGG TGCTA (original)
________________________________________________________ (new)

_________________________________________________________ (new)
________________________________________________________ (compl. from 2A)

3. A. Original DNA:TGCTG ATCGA TCGAT CAGTC AAACG CTGTT TCGAT ACTCG

Complementary DNA: _______________________________________________________________________

B. Make identical strands of DNA


TGCTG ATCGA TCGAT CAGTC AAACG CTGTT TCGAT ACTCG (original)
__________________________________________________________ (new)

_________________________________________________________ (new)
________________________________________________________ (compl. from 3A)

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