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    PA2 Value and PD2 Value

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    guddu1680
    The document describes experiments to determine the PA2 value of prazosin using rat anococcygeus muscle tissue and the PD2 value of histamine using guinea pig ileum tissue. It involves mounting the tissues, recording dose response curves to agonists, and adding increasing concentrations of antagonists to determine the dose needed to halve the agonist response.

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    PA2 Value and PD2 Value

    Uploaded by

    guddu1680
    408 views4 pages
    The document describes experiments to determine the PA2 value of prazosin using rat anococcygeus muscle tissue and the PD2 value of histamine using guinea pig ileum tissue. It involves mounting the tissues, recording dose response curves to agonists, and adding increasing concentrations of antagonists to determine the dose needed to halve the agonist response.

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    Third Year B.Pharma

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    The document describes experiments to determine the PA2 value of prazosin using rat anococcygeus muscle tissue and the PD2 value of histamine using guinea pig ileum tissue. It involves mounting the tissues, recording dose response curves to agonists, and adding increasing concentrations of antagonists to determine the dose needed to halve the agonist response.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    408 views4 pages

    PA2 Value and PD2 Value

    Uploaded by

    guddu1680
    The document describes experiments to determine the PA2 value of prazosin using rat anococcygeus muscle tissue and the PD2 value of histamine using guinea pig ileum tissue. It involves mounting the tissues, recording dose response curves to agonists, and adding increasing concentrations of antagonists to determine the dose needed to halve the agonist response.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    Aim: Determination of PA2 value of prazosin using rat anococcygeus muscle (by Schilds plot

    method).

    1. Mount the tissue on tissue organ bath with Tyrode solution

    2. After relaxation of tissue, record DRC of noradrenaline

    A1-A5 is 0.1, 0.2, 0.4, 0.8, 1.6 ml dose of Noradrenaline (100 ug/ml) respectively,

    3. Select one dose norepinephrine which falls between 25-75% in DRC label it as 2A.

    4. Record 2A response norepinephrine in presence of increase concentration of antagonist i.e.


    prazocin till 2A become A or less than A.

    A=0.2ml

    2A=0.4ml

    B1= 5 ug/ml prazosin after 15 min add 0.4 ml of 2A,then measured response height. After
    measuring response without washing add 2A after 5 min time cycle , then again added 2A(0.4 ml) to
    get same height it indicate antagonist removed from tissue.

    B2=8 ug/ml prazosin after 15 min add 0.4 ml of 2A,then measured response height. After
    measuring response without washing add 2A after 5 min time cycle , then again added 2A(0.4 ml) to
    get same height it indicate antagonist removed from tissue.
    B3=11ug/ml prazosin after 15 min add 0.4 ml of 2A,then measured response height. After
    measuring response without washing add 2A after 5 min time cycle , then again added 2A(0.4 ml) to
    get same height it indicate antagonist removed from tissue.

    DRC of norepinephrine (100ug/ml)

    Sr.No. Dose(ml) Dose ug Log dose Response(mm) % Response


    A1 0.1 10 1.0000 5 19.23
    A2 0.2 20 1.3010 10 38.46
    A3 0.4 40 1.6020 20 76.92
    A4 0.8 80 1.9030 25 96.15
    A5 1.6 160 2.2041 26 100

    Dose Dose(ml) Response(mm) % Response


    2A 0.4 20 100
    B1-2A (5ug/ml) 0.00017 18 90

    B2- 2A (8ug/ml) 0.00028 15 75

    B3 -2A(11ug/ml) 0.00038 10 50

    For example ; 5ug=1ml


    X ug=35 ml organ tube
    So , X=35x5=175
    Molar conc.=conc.in organ tube/mol.wt=175/383.4=0.456

    Dose of Molar concentration Log molar conc. % Response


    antagonist
    0.00017 0.456 -0.341035157 90
    0.00028 0.730 -0.13667714 75
    0.00038 1.00 0 50
    Aim: Determination of PD2 value of Histamine using guinea pig ileum.

    Dose Response curve of Histamine (100ug/ml)

    1. Make observation table as like DRC


    2. Plot log dose response versus % response curve.
    3. Determine dose of histamine which produce 50% response .
    For example
    you get the value from graph at 50% response suppose 1.04
    Take antilog(1.04)= 10.96
    Concentration 100ug/ml
    1 ml=100 ug
    X= 10.96
    X=10.96/100=0.1096
    Next step
    To determine concentration in of histamine in organ tube which produce 50% response

    Y=Quantity in ml to produce 50% response(x) X Concentration of histamine used

    Bath capacity (ml)

    Y=01096 X 100/32
    Y=0.3406 ug/ml

    Next step
    Molar concentration =concentration which produce 50% response /molecular weight X 1000
    = 0.3406/111.15X1000
    =0.003064/1000
    =3.064X10^-6
    pD2 Value =log[3.064X10^-6]
    =6- log[3.064]
    =6-0.4862
    =5.5138

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