c at V e r s i o n

Elaine N. Marieb, R.N., Ph.D.

Holyoke Community College

Lori A. Smith, Ph.D.

American River College

Human
Anatomy & Physiology
Laboratory Manual
T w e l f t h E d i t i o n

PhysioEx™ Version 9.1 authored by

Peter Z. Zao North Idaho College
Timothy Stabler, Ph.D. Indiana University Northwest
Lori A. Smith, Ph.D. American River College
Andrew Lokuta, Ph.D. University of Wisconsin–Madison
Edwin Griff, Ph.D. University of Cincinnati

MARI1357_12_CAT_FM_ppi-xii.indd 1 11/7/14 3:10 PM

 Editor-in-Chief: Serina Beauparlant Art and Photo Coordinator: David Novak
Senior Acquisitions Editor: Brooke Suchomel Photo Researcher: Kristin Piljay
Program Manager: Shannon Cutt Interior and Cover Designer: tani hasegawa
Program Manager Team Lead: Michael Early Copyeditor: Sally Peyrefitte
Director of Development: Barbara Yien Compositor: Cenveo® Publisher Services
Development Editor: Lisa Clark Media Producers: Liz Winer and Lauren Hill
Editorial Assistant: Arielle Grant PhysioEx Developer: BinaryLabs, Inc.
Production Project and Design Manager: Michele Mangelli Senior Manufacturing Buyer: Stacey Weinberger
Project Manager Team Lead: Nancy Tabor Senior Marketing Manager: Allison Rona
Production Supervisor: Janet Vail Senior Anatomy & Physiology Specialist: Derek Perrigo

Cover photographs: Pole vaulter, Pete Saloutos/Getty Images; Sky, Gregor Schuster/Getty Images

Acknowledgments of third-party content appear on page BM-1, which constitutes an extension of this
copyright page.

Copyright © 2016, 2014, 2012. Pearson Education, Inc. All Rights Reserved. Printed in the United
States of America. This publication is protected by copyright, and permission should be obtained from
the publisher prior to any prohibited reproduction, storage in a retrieval system, or transmission in any
form or by any means, electronic, mechanical, photocopying, recording, or otherwise. For information
regarding permissions, request forms and the appropriate contacts within the Pearson Education Global
Rights & Permissions department, please visit www.pearsoned.com/permissions/.

PEARSON, ALWAYS LEARNING, MasteringA&P®, A&PFlix™, PAL™, and PEx™ are exclusive
trademarks, in the U.S. and/or other countries, owned by Pearson Education, Inc. or its affiliates.

Unless otherwise indicated herein, any third-party trademarks that may appear in this work are the
property of their respective owners and any references to third-party trademarks, logos or other trade
dress are for demonstrative or descriptive purposes only. Such references are not intended to imply any
sponsorship, endorsement, authorization, or promotion of Pearson’s products by the owners of such
marks, or any relationship between the owner and Pearson Education, Inc. or its affiliates, authors,
licensees or distributors.

The Authors and Publisher believe that the lab experiments described in this publication, when conducted
in conformity with the safety precautions described herein and according to the school’s laboratory safety
procedures, are reasonably safe for the student to whom this manual is directed. Nonetheless, many of
the described experiments are accompanied by some degree of risk, including human error, the failure or
misuses of laboratory or electrical equipment, mismeasurement, chemical spills, and exposure to sharp
objects, heat, bodily fluids, blood, or other biologics. The Authors and Publisher disclaim any liability
arising from such risks in connection with any of the experiments contained in this manual. If students have
any questions or problems with materials, procedures, or instructions on any experiment, they should always
ask their instructor for help before proceeding.

ISBN 10: 0-321-97135-3 (student edition)

ISBN 13: 978-0-321-97135-7 (student edition)
ISBN 10: 0-13-399927-0 (instructor’s review copy)
ISBN 13: 978-0-13-399927-3 (instructor’s review copy)
www.pearsonhighered.com 1 2 3 4 5 6 7 8 9 10—V364—18 17 16 15 14

MARI1357_12_CAT_FM_ppi-xii.indd 2 11/19/14 9:55 AM

 Contents iii

Contents
THE HUMAN BODY: AN ORIENTATION
55 The Cell: Transport Mechanisms
11
and Cell Permeability 53
The Language of Anatomy 1
1 Observing Diffusion of Dye Through
1 Locating Body Regions 3 Agar Gel 55
2 Practicing Using Correct Anatomical 2 Observing Diffusion of Dye
Terminology 4 Through Water 56
3 Observing sectioned Specimens 6 3 Investigating Diffusion and Osmosis
4 Identifying Organs in the Abdominopelvic Through Nonliving Membranes 56
Cavity 8 4 Observing Osmometer Results 58
5 Locating Abdominal Surface Regions 8 5 Investigating Diffusion and Osmosis
Group Challenge The Language of Anatomy 10 Through Living Membranes 58
Review Sheet 11 6 Observing the Process of Filtration 60
7 Observing Phagocytosis 62
22 Organ Systems Overview 15 Group Challenge Compare and Contrast Membrane
Transport Processes 62
1 Observing External Structures 17
2 Examining the Oral Cavity 17 Review Sheet 63
3 Opening the Ventral Body Cavity 17
4 Examining the Ventral Body Cavity 18 HISTOLOGY: BASIC TISSUES
5 Examining the Human Torso Model 23 OF THE BODY
Group Challenge Odd Organ Out 24
Review Sheet 25 66 Classification of Tissues 67
1 Examining Epithelial Tissue Under the
THE MICROSCOPE AND ITS USES Microscope 74
Group Challenge 1 Identifying Epithelial

33
Tissues 74
The Microscope 27 2 Examining Connective Tissue Under the
1 Identifying the Parts of a Microscope 28 Microscope 76
2 Viewing Objects Through the Microscope 29 3 Examining Nervous Tissue Under the
3 Estimating the Diameter of the Microscope Microscope 82
Field 32 4 Examining Muscle Tissue Under the
4 Perceiving Depth 33 Microscope 83
5 Preparing and Observing a Wet Mount 33 Group Challenge 2 Identifying Connective
Review Sheet 35 Tissue 86
Review Sheet 87

THE cell
THE INTEGUMENTARY SYSTEM
44 The Cell: Anatomy and Division 39
1 Identifying Parts of a cell 40 77 The Integumentary System 93
2 Identifying Components 1 Locating Structures on a Skin Model 95
of a Plasma Membrane 41 2 Identifying Nail Structures 98
3 Locating Organelles 42 3 Comparing Hairy and Relatively Hair-
4 Examining the Cell Model 42 free Skin Microscopically 98
5 Observing Various Cell Structures 43 4 Differentiating Sebaceous and Sweat
Glands Microscopically 100
6 Identifying the Mitotic Stages 45
5 Plotting the Distribution of
7 “Chenille Stick” Mitosis 45 Sweat Glands 100
Review Sheet 49 6 Taking and Identifying Inked
Fingerprints 101
Review Sheet 103

iii

MARI1357_12_CAT_FM_ppi-xii.indd 3 11/7/14 3:10 PM

 iv Contents

THE SKELETAL SYSTEM THE MUSCULAR SYSTEM

88 Overview of the Skeleton: 12 Microscopic Anatomy and Organization

12
Classification and Structure of Skeletal Muscle 189
of Bones and Cartilages 107 1 Examining Skeletal Muscle Cell Anatomy 192
1 Examining a Long Bone 111 2 Observing the Histological Structure
2 Examining the Effects of Heat and Hydrochloric of a Skeletal Muscle 192
Acid on Bones 112 3 Studying the Structure of a Neuromuscular
3 Examining the Microscopic Structure Junction 193
of Compact Bone 113 Review sheet 195
4 Examining the Osteogenic Epiphyseal Plate 115
Review Sheet 117 13 Gross Anatomy of the Muscular
13
System 199
99 The Axial Skeleton 121 1 Identifying Head and Neck Muscles 201
1 Identifying the Bones of the Skull 122 2 Identifying Muscles of the Trunk 201
Group Challenge Odd Bone Out 131 3 Identifying Muscles of the Upper Limb 215
2 Palpating Skull Markings 133 4 Identifying Muscles of the Lower Limb 220
3 Examining Spinal Curvatures 134 Group Challenge Name that Muscle 224
4 Examining Vertebral Structure 138 5 Review of Human Musculature 226
5 Examining the Relationship Between 6 Making a Muscle Painting 226
Ribs and Vertebrae 139 Review sheet 229
6 Examining a Fetal Skull 140
Review sheet 141 14 Skeletal Muscle Physiology: Frogs
14
and Human Subjects 237
10 The Appendicular Skeleton
10 149 1 Observing Muscle Fiber Contraction 238
1 Examining and identifying Bones of the 2 Inducing Contraction in the Frog
Appendicular Skeleton 150 Gastrocnemius Muscle 240
2 Palpating the Surface Anatomy of the Pectoral 3 Demonstrating Muscle Fatigue in Humans 245
Girdle and upper Limb 152
BIOPAC 4 Electromyography in a Human Subject
®

3 Observing Pelvic Articulations 155 Using BIOPAC® 246

4 Comparing Male and Female Pelves 157 Review sheet 253
5 Palpating the Surface Anatomy of the Pelvic
Girdle and Lower Limb 161
6 Constructing a Skeleton 162
THE NERVOUS SYSTEM
Review sheet 163
15 Histology of Nervous Tissue
15 257
11 Articulations and Body
11 1 Identifying Parts of a Neuron 260
2 Studying the Microscopic Structure
Movements 171 of Selected Neurons 262
1 Identifying Fibrous Joints 172 3 Examining the Microscopic Structure
2 Identifying Cartilaginous Joints 172 of a Nerve 264
3 Examining Synovial Joint Structure 174 Review sheet 265
4 Demonstrating the Importance
of Friction-Reducing Structures 174
5 Demonstrating Movements of Synovial
16 Neurophysiology of Nerve Impulse:
16
Joints 176
Frog Subjects 269
1 Stimulating the Nerve 272
6 Demonstrating Actions at the Hip Joint 178
2 Inhibiting the Nerve 273
7 Demonstrating Actions at the Knee Joint 179
3 Visualizing the Compound Action
8 Demonstrating Actions at the Shoulder
Potential with an Oscilloscope 274
Joint 181
Review sheet 277
9 Examining the Action at the TMJ 182
Group Challenge Articulations: “Simon Says” 184
Review sheet 185

MARI1357_12_CAT_FM_ppi-xii.indd 4 11/7/14 3:10 PM

 Contents v

6 Demonstrating the Phenomenon of Referred

17 Gross Anatomy of the Brain and Cranial
17 Pain 358
Nerves 279 Review Sheet 359
1 Identifying External Brain Structures 281
2 Identifying Internal Brain Structures 283 23 Special Senses: Anatomy of the Visual
23
3 Identifying and Testing the Cranial Nerves 288 System 361
Group Challenge Odd (cranial) Nerve Out 296 1 Identifying Accessory Eye Structures 363
Review sheet 297 2 Identifying Internal Structures of the Eye 363
3 Studying the Microscopic Anatomy of the
18 Electroencephalography
18 303 Retina 364
4 Predicting the Effects of Visual Pathway
1 Observing Brain Wave Patterns Using
an Oscilloscope or Physiograph 304 Lesions 368
BIOPAC 2 Electroencephalography Using BIOPAC® 305
®
Review Sheet 369

24 Special Senses: Visual Tests and

Review sheet 309
24
19 The Spinal Cord and Spinal Nerves
19 311 Experiments
1
373
Demonstrating the Blind Spot 373
1 Identifying Structures of the Spinal Cord 312
2 Identifying Spinal Cord Tracts 315 2 Determining Near Point of Accommodation 374
3 Identifying the Major Nerve Plexuses and 3 Testing Visual Acuity 375
Peripheral Nerves 322 4 Testing for Astigmatism 376
Group Challenge Fix the Sequence 322 5 Testing for Color Blindness 376
Review sheet 323 6 Testing for Depth Perception 377
7 Demonstrating Reflex Activity of Intrinsic
20 The Autonomic Nervous System
20 327 and Extrinsic Eye Muscles 377
8 Conducting an Ophthalmoscopic Examination 378
1 Locating the Sympathetic trunk 328
Review Sheet 379
2 Comparing Sympathetic and Parasympathetic
Effects 330
BIOPAC 3 Exploring the Galvanic skin Response
®

25 Special Senses: Hearing and

25
(Electrodermal Activity) within a Polygraph Equilibrium 383
Using BIOPAC® 330
1 Identifying Structures of the Ear 384
Review sheet 337
2 Examining the Ear with an Otoscope

21 Human Reflex Physiology

(Optional) 386
21 339 3 Examining the Microscopic Structure of the
1 Initiating Stretch Reflexes 341 Cochlea 387
2 Initiating the Crossed-Extensor Reflex 343 4 Conducting Laboratory Tests of
Hearing 387
3 Initiating the Plantar Reflex 343
4 Initiating the Corneal Reflex 344
5 Audiometry Testing 390
5 Initiating the Gag Reflex 344
6 Examining the Microscopic Structure of the
Crista Ampullaris 391
6 Initiating Pupillary Reflexes 345
7 Conducting Laboratory Tests on Equilibrium 391
7 Initiating the Ciliospinal Reflex 345
Review Sheet 395
8 Initiating the Salivary Reflex 345
9 Testing Reaction Time for Intrinsic
and Learned Reflexes 346 26 Special Senses: Olfaction and
26
BIOPAC 10 Measuring Reaction Time Using BIOPAC® 347
®
Taste 399
Review Sheet 349 1 Microscopic Examination of the Olfactory
Epithelium 401

22 General Sensation
22 353
2 Microscopic Examination of Taste Buds 402
3 Stimulating Taste Buds 402
1 Studying the Structure of Selected
Sensory Receptors 355
4 Examining the Combined Effects of Smell,
Texture, and Temperature on Taste 402
2 Determining the Two-Point Threshold 356
5 Assessing the Importance of Taste and
3 Testing Tactile Localization 357 Olfaction in Odor Identification 404
4 Demonstrating Adaptation of Touch 6 Demonstrating Olfactory Adaptation 404
Receptors 357
Review Sheet 405
5 Demonstrating Adaptation of Temperature
Receptors 357

MARI1357_12_CAT_FM_ppi-xii.indd 5 11/7/14 3:10 PM

 vi Contents

THE ENDOCRINE SYSTEM

32 Anatomy of Blood Vessels
32 471
27 Functional Anatomy of the Endocrine
27
1 Examining the Microscopic Structure of Arteries
and Veins 473
Glands 407 2 Locating Arteries on an Anatomical Chart or
1 Identifying the Endocrine Organs 408 Model 480
2 Examining the Microscopic Structure of 3 Identifying the Systemic Veins 484
Endocrine Glands 412 4 Identifying Vessels of the Pulmonary
Group Challenge Odd Hormone Out 414 Circulation 485
Review Sheet 415 Group Challenge Fix the Blood Trace 486
5 Tracing the Pathway of Fetal Blood Flow 486
28 Endocrine Wet Labs and Human
28 6 Tracing the Hepatic Portal Circulation 488
Metabolism 419 Review Sheet 489
1 Determining the Effect of Pituitary Hormones
on the Ovary 420
2 Observing the Effects of Hyperinsulinism 421
33 Human Cardiovascular Physiology:
33
Blood Pressure and Pulse
Group Challenge Thyroid Hormone Case Studies 422 Determinations 495
Review Sheet 423 1 Auscultating Heart Sounds 498
2 Palpating Superficial Pulse Points 499
THE CIRCULATORY SYSTEM BIOPAC 3
®

Measuring Pulse Using BIOPAC® 500

4 Taking an Apical Pulse 502
29 Blood
29 425 5 Using a Sphygmomanometer to Measure
1 Determining the physical Characteristics of Arterial Blood Pressure Indirectly 502
Plasma 428 6 Estimating venous Pressure 503
2 Examining the Formed Elements of Blood 7 Observing the Effect of Various Factors
Microscopically 428 on Blood Pressure and Heart Rate 504
3 Conducting a Differential WBC Count 431 8 Examining the Effect of Local Chemical and
4 Determining the Hematocrit 432 Physical Factors on Skin Color 506
5 Determining Hemoglobin Concentration 434 Review Sheet 509
6 Determining Coagulation Time 435
7 Typing for ABO and Rh Blood Groups 436 34 Frog Cardiovascular Physiology
34 515
8 Observing Demonstration Slides 438 1 Investigating the Automaticity and Rhythmicity
9 Measuring Plasma Cholesterol Concentration 438 of Heart Muscle 516
BIOPAC 2 Recording Baseline Frog Heart Activity 518
®

Review Sheet 439

3 Investigating the Refractory Period of Cardiac
30 Anatomy of the Heart
30 445
Muscle Using the Physiograph 521
BIOPAC 4 Assessing Physical and Chemical Modifiers of
®

1 Using the Heart Model to Study Heart Heart Rate 521

Anatomy 448
5 Investigating the Effect of Various Factors
2 Tracing the Path of Blood Through the Heart 449 on the Microcirculation 523
3 Using the Heart Model to Study Cardiac Review Sheet 525
Circulation 450
4 Examining Cardiac Muscle Tissue Anatomy 451
Review Sheet 455
35 The Lymphatic System and Immune
35
Response 529
31 Conduction System of the Heart
31
1 Identifying the Organs of the Lymphatic
System 531
and Electrocardiography 459 2 Studying the Microscopic Anatomy of a Lymph
1A Recording ECGs Using a Standard ECG Node, the Spleen, and a Tonsil 532
Apparatus 463 Group Challenge Compare and Contrast Lymphoid
BIOPAC 1B Electrocardiography Using BIOPAC® 464 Organs and Tissues 534
®

Review Sheet 469 3 Using the Ouchterlony Technique to Identify

Antigens 535
Review Sheet 537

MARI1357_12_CAT_FM_ppi-xii.indd 6 11/7/14 3:10 PM

 Contents vii

THE RESPIRATORY SYSTEM 4 Reporting Results and Conclusions 605

Group Challenge Odd Enzyme Out 605
36 Anatomy of the Respiratory
36 5 Observing Movements and sounds of the
Digestion System 606
system 541
6 Viewing Segmental and Peristaltic
1 Identifying Respiratory System Organs 547 Movements 607
2 Demonstrating Lung Inflation in a Sheep Review Sheet 609
Pluck 547
3 Examining Prepared Slides of Trachea and Lung
Tissue 547 THE URINARY SYSTEM
40 Anatomy of the Urinary System
Review Sheet 549
40 613
37 Respiratory System Physiology
37 553 1 Identifying Urinary System Organs 615
2 Studying Nephron Structure 618
1 Operating the Model Lung 554
2 Auscultating Respiratory Sounds 556 3 Studying Bladder Structure 621
3 Measuring Respiratory Volumes Using Group Challenge Urinary System Sequencing 622
Spirometers 557 Review Sheet 623
4 Measuring the FVC and FEV1 563
BIOPAC 5 Measuring Respiratory Volumes Using
®

BIOPAC® 563
41 Urinalysis
41 627
1 Analyzing Urine Samples 629
6 Visualizing Respiratory Variations 567 2 Analyzing Urine Sediment Microscopically
7 Demonstrating the Reaction Between (Optional) 632
Carbon Dioxide (in Exhaled Air) Review Sheet 633
and Water 569
8 Observing the Operation of Standard
Buffers 570 THE REPRODUCTIVE SYSTEM,
9 Exploring the Operation of the Carbonic DEVELOPMENT, AND HEREDITY
Acid-Bicarbonate Buffer System 570
Review Sheet 571 42 Anatomy of the Reproductive
42
System 635
THE DIGESTIVE SYSTEM 1 Identifying Male Reproductive Organs 636
2
38 Anatomy of the Digestive System
Penis 639
38 577 3 Seminal Gland 640
1 Identifying Alimentary Canal Organs 578 4 Epididymis 640
2 Studying the Histologic Structure of the Stomach 5 Identifying Female Reproductive Organs 640
and the Gastroesophageal Junction 583
6 Wall of the Uterus 643
3 Observing the Histologic Structure 7 Uterine Tube 643
of the Small Intestine 586
Review Sheet 645
4 Examining the Histologic Structure
of the Large Intestine 588
5 Identifying Types of Teeth 589 43 Physiology of Reproduction:
43
6 Studying Microscopic Tooth Anatomy 590 Gametogenesis and the Female
7 Examining Salivary Gland Tissue 590 Cycles 651
8 Examining the Histology of the Liver 591 1 Identifying Meiotic phases and structures 653
Review Sheet 593 2 Examining Events of Spermatogenesis 653
3 Examining Meiotic Events Microscopically 655
39 Digestive System Processes: Chemical
39 4 Examining Oogenesis in the Ovary 656
and Physical 599 5 Comparing and Contrasting Oogenesis and
Spermatogenesis 656
1 Assessing Starch Digestion by Salivary
Amylase 600 6 Observing Histological Changes in the
Endometrium During the Menstrual Cycle 658
2 Assessing Protein Digestion by Trypsin 603
Review sheet 661
3 Demonstrating the Emulsification
Action of Bile and Assessing Fat Digestion
by Lipase 604

MARI1357_12_CAT_FM_ppi-xii.indd 7 11/7/14 3:10 PM

 viii Contents

44 Survey of Embryonic
44 CAT DISSECTION EXERCISES
Development 665
1 Microscopic Study of Sea Urchin
1 Dissection and Identification
 Cat Muscles 705
of
Development 666
2 Examining the Stages of Human 2 Dissection of Cat Spinal Nerves 723
Development 666
3 Identifying Fetal Structures 669
3 Identification of Selected Endocrine
Organs of the Cat 729
4 Studying Placental Structure 670
Review sheet 671 4 Dissection of the Blood Vessels
of the Cat 733
45 principles of heredity
45 675 5 The Main Lymphatic Ducts of the Cat 743
1 Working Out Crosses Involving Dominant and
Recessive Genes 676
6 Dissection of the Respiratory System
of the Cat 745
2 Working Out Crosses Involving Incomplete
Dominance 677 7 Dissection of the Digestive System
3 Working Out Crosses Involving Sex-Linked of the Cat 749
Inheritance 678
4 Exploring Probability 679 8 Dissection of the Urinary System
of the Cat 755
5 Using Phenotype to Determine Genotype 679
6 Using Agarose Gel Electrophoresis to Identify 9 Dissection of the Reproductive System
Normal Hemoglobin, Sickle Cell Anemia, and of the Cat 759
Sickle Cell Trait 681
Group Challenge Odd Phenotype Out 682 PHYSIOEX TM 9.1 COMPUTER SIMULATIONS
Review Sheet 683
1 Cell Transport Mechanisms and
Permeability PEx-3
SURFACE ANATOMY
2 Skeletal Muscle Physiology PEx-17
46 Surface Anatomy Roundup
46 687 3 Neurophysiology of Nerve Impulses PEx-35
1
2
Palpating Landmarks of the Head 688
Palpating Landmarks of the Neck 690
4 Endocrine System Physiology PEx-59

3 Palpating Landmarks of the Trunk 692 5 Cardiovascular Dynamics PEx-75

4 Palpating Landmarks of the Abdomen 694 6 Cardiovascular Physiology PEx-93
5
6
Palpating Landmarks of the Upper Limb 696
Palpating Landmarks of the Lower Limb 699
7 Respiratory System Mechanics PEx-105

Review Sheet 703 8 Chemical and Physical Processes

of Digestion PEx-119

9 Renal System Physiology PEx-131

10 Acid-Base Balance PEx-149

11 Blood Analysis PEx-161

12 Serological Testing PEx-177

Credits BM-1
index BM-3

MARI1357_12_CAT_FM_ppi-xii.indd 8 11/7/14 3:10 PM

 ELAINE MARIEB is the
most trusted name in all of
A&P. More than 3 million
healthcare professionals
started their careers with
one of Elaine Marieb’s
Anatomy & Physiology texts.

Now, it’s your turn.

MARI1357_12_CAT_FM_ppi-xii.indd 9 11/19/14 10:49 AM

 Tools to Help You
NEW!
Why This Matters shows you why the lab activity is important by relating
it to a real-life or clinical example. In the Review Sheets, corresponding
Why This Matters questions help you evaluate your understanding.

>
74 Exercise 6

Activity 1
Examining Epithelial Tissue Under the Microscope
Obtain slides of simple squamous, simple cuboidal, sim-
ple columnar, stratified squamous (nonkeratinized), pseu- Buccal Swabs
dostratified ciliated columnar, stratified cuboidal, stratified
columnar, and transitional epithelia. Examine each care- A buccal, or cheek, swab is a method used to collect
fully, and notice how the epithelial cells fit closely together stratified squamous cells from the oral cavity. The
6 to form intact sheets of cells, a necessity for a tissue that cells contain DNA that can be used for DNA finger-
forms linings or the coverings of membranes. Scan each printing or tissue typing. DNA fingerprinting can be

NEW! epithelial type for modifications for specific functions,

such as cilia (motile cell projections that help to move
substances along the cell surface), and microvilli, which
used in criminal investigations, and tissue typing can
be used to match a recipient with a donor for or-
gan transplant, especially a bone marrow transplant.
increase the surface area for absorption. Also be alert for The buccal swab procedure involves using a cotton-
The new goblet cells, which secrete lubricating mucus. Compare tipped applicator to scrape the inside of the mouth
your observations with the descriptions and photomicro- in the buccal region and remove cells at the surface.
workbook-style graphs in Figure 6.3. This noninvasive procedure provides an easy way
While working, check the questions in the Review to obtain the DNA profile of an individual, a unique
design makes Sheet at the end of this exercise. A number of the ques- molecular “signature.” ■
tions there refer to some of the observations you are
it easy to see asked to make during your microscopic study.

where sections,
lab activities, and
Group Challenge 1
drawing activities
Identifying Epithelial Tissues
begin and end. Following your observations of epithelial tissues under the Now, carefully go through each group and try to
microscope, obtain an envelope for each group that contains list one place in the body where the tissue is found
images of various epithelial tissues. With your lab manual and one function for it. After you have correctly listed
closed, remove one image at a time and identify the epithe- the locations, take your lists and draw some general
lium. One member of the group will function as the verifier, conclusions about where epithelial tissues are found in
whose job is to make sure that the identification is correct. the body. Then compare and contrast the functions of the
After you have correctly identified all of the images, various epithelia. Finally, identify the tissues described in
sort them into groups to help you remember them. (Hint: the Group Challenge 1 chart, and list several locations
You could sort them according to cell shape or number of in the body.
layers of epithelial cells.)

Group Challenge 1: Epithelial Tissue IDs

Magnified appearance Tissue type Locations in the body

• Apical surface has dome-shaped cells

(flattened cells may also be mixed in)
• Multiple layers of cells are present

• Cells are mostly columnar

• Not all cells reach the apical surface
• Nuclei are located at different levels
• Cilia are located at the apical surface

• Apical surface has flattened cells with very

little cytoplasm
• Cells are not layered

• Apical surface has square cells with a

round nucleus
• Cells are not layered

MARI1357_12_C06_pp067-092.indd 74 9/11/14 5:09 PM

MARI1357_12_CAT_FM_ppi-xii.indd 10 11/7/14 3:10 PM

 Succeed in the Lab
NEW!
Prepare for lab by watching pre-lab videos on
your smartphone, tablet, or computer. Short
pre-lab videos will give you some background
information on the lab, explain what materials you
will be using, help you see the connections between
the content you learn in the classroom and the
experiment, and give you the opportunity to check your Prepare for lab: Watch the Pre-lab Video
>Study Area>Pre-Lab Videos
understanding—all before you even step foot in the lab.

NEW!
Dynamic Study Modules offer a mobile-friendly,
personalized reading experience of the chapter content.
As you answer questions to master the chapter content,
you receive detailed feedback with text and art from the
lab manual itself. The Dynamic Study Modules help you
acquire, retain, and recall information faster and more
efficiently than ever before.

Practice Anatomy Lab™ (PAL™) 3.0 is a PhysioEx™ 9.1 is an easy-to-use lab simulation
virtual anatomy study and practice tool that program that allows you to repeat labs as often as
gives you 24/7 access to the most widely used you like, perform experiments without animals,
lab specimens, including the human cadaver, and conduct experiments that are difficult to
anatomical models, histology, cat, and fetal pig. perform in a wet lab environment because of time,
PAL 3.0 is easy to use and includes built-in audio cost, or safety concerns. The online format with
pronunciations, rotatable bones, and simulated easy step-by-step instructions includes everything
fill-in-the-blank lab practical exams. you need in one convenient place.

MARI1357_12_CAT_FM_ppi-xii.indd 11 11/7/14 3:10 PM

 Acknowledgments

We wish to thank the following reviewers for their contribution Shannon Cutt, Program Manager. Applause also to Lauren Hill
to this edition: Matthew Abbott, Des Moines Area Community who managed MasteringA&P, and Aimee Pavy for her work
College; Lynne Anderson, Meridian Community College; on PhysioEx 9.1. Many thanks to Amanda J.S. Kaufmann who
Christopher W. Brooks, Central Piedmont Community did a super job as video production manager of the new pre-lab
College; Brandi Childress, Georgia Perimeter College; videos. Additional thanks go to Cheryl Chi and the group at
Christopher D'Arcy, Cayuga Community College; Mary E. Roaring Mouse Productions, who provided their special skills to
Dawson, Kingsborough Community College; Karen Eastman, the video production team. Many thanks to Stacey Weinberger
Chattanooga State Community College; Michele Finn, Monroe for her manufacturing expertise. Finally, our Senior Marketing
Community College; Lisa Flick, Monroe Community College; Manager, Allison Rona, has efficiently kept us in touch with the
Abigail M. Goosie, Walters State Community College; Samuel pulse of the market.
Hirt, Auburn University; Shahdi Jalilvand, Tarrant County Kudos also to Michele Mangelli and her production team,
College—Southeast; Tiffany B. McFalls-Smith, Elizabethtown who did their usual great job. Janet Vail, Production Editor for
Community & Technical College; Melinda Miller, Pearl this project, got the job done in jig time. David Novak acted as
River Community College; Todd Miller, Hunter College Art and Photo Coordinator, and Kristin Piljay conducted photo
of CUNY; Susan Mitchell, Onondaga Community College; research. Our fabulous interior and cover designs were created
Erin Morrey, Georgia Perimeter College; Jill Y. O'Malley, Erie by tani hasegawa. Sally Peyrefitte brought her experience to
Community College—City; Suzanne Oppenheimer, College copyediting the text.
of Western Idaho; Suzanne Pundt, The University of Texas at We are grateful to the team at BIOPAC, especially to Jocelyn
Tyler; Mark Schmidt, Clark State Community College; Teresa Kremer and Mike Mullins, who were extremely helpful in
Stegall-Faulk, Middle Tennessee State University; Bonnie J. making sure we had the latest updates and answering all of our
Tarricone, Ivy Tech Community College. questions.
Special thanks to Susan Mitchell for her authorial contribu-
tions to this lab manual over the years.
Thanks also to Josephine Rogers of the University of
Cincinnati, the original author of the pre-lab quizzes.
The excellence of PhysioEx 9.1 reflects the expertise of
Peter Zao, Timothy Stabler, Lori Smith, Andrew Lokuta, Greta
Peterson, Nina Zanetti, and Edwin Griff. They generated the
ideas behind the activities and simulations. Credit also goes
to the team at BinaryLabs, Inc., for their expert programming Elaine N. Marieb
and design. Lori A. Smith
Continued thanks to colleagues and friends at Pearson who Anatomy and Physiology
worked with us in the production of this edition, especially Pearson Education
Serina Beauparlant, Editor-in-Chief; Brooke Suchomel, Senior 1301 Sansome Street
Acquisitions Editor; Lisa Clark, Development Editor; and San Francisco, CA 94111

MARI1357_12_CAT_FM_ppi-xii.indd 12 11/18/14 1:59 PM