Agilent 6530 Accurate-Mass

Q-TOF LC/MS

Data Sheet

The 6530 Accurate-Mass Q-TOF achieves sensitivity, mass accuracy, mass resolution and scan speed specifications after autotune without
manual user intervention.

Parameter Measure Specification

Sensitivity, MS mode, electrospray on-column, 1 pg LC/MS injection of reserpine. 10:1 RMS (typical 30:1 RMS)
400 µL/min Signal-to-noise for the reserpine (M+H)+ at
m/z 609.2807
Sensitivity, MS/MS mode, electrospray 1 pg LC/MS injection of reserpine. 50:1 RMS (typical 200:1 RMS)
on-column, 400 µL/min Signal-to-noise for most intense product ions
(174, 195,397,448 m/z)
Sensitivity, MS mode, Nanoflow ChipMS in 500 attomole of BSA peptide digest 15% coverage in protein database
electrospray, 300 nL/min on column (200 attomole- typical) search (PMF)
Sensitivity, MS/MS mode, Nanoflow ChipMS in 500 attomole of BSA peptide digest High confidence match via protein data
electrospray, 300 nL/min on column (200 attomole- typical) base search (Mass tag)
Sensitivity, MS/MS mode, Infusion using 100 fmol/ul Glu-fib peptide, 6 second Most intense fragment ion gives
nanoflow ChipMS in electrospray, accumulation time (500 attomole/sec consumed) 5,000 counts/sec and 50:1 S/N RMS
300 nL/min on column
Mass resolving power Measured at m/z 1,522 after automatic Greater than 20,000 FWHM
tuning procedure (typical 24,000 FWHM)
Measured at m/z 118 after automatic Greater than 10,000 FWHM
tuning procedure Note – W optics not required to achieve
resolution specifications
Parameter Measure Specification
Mass accuracy – MS mode, electrospray Measured at the (M+H)+ ion of reserpine Better than 2 ppm RMS as measured from
on-column, 400 µL/min (m/z 609.2807) using an internal mass 10 repeat injections (typical 1 to 2 ppm)
reference – 20 pg LC/MS injection
Mass accuracy – MS/MS mode, Product ion 397 m/z for reserpine – 20 pg Better than 5 ppm RMS on m/z 397 as
electrospray on-column, 400 µL/min LC/MS injection measured from 10 repeat injections
Mass accuracy – MS mode, Infusion using Measured at the (M+2H)++ ion of Glu-fib Better than 2 ppm RMS as measured from
nanoflow ChipMS in electrospray, peptide (m/z 785.83) using an internal mass ten 6 second spectra
300 nL/min on column reference – 100 fmol/ul with 6 second
accumulation (3 fmol consumed)
Mass accuracy – MS/MS mode, Infusion Product ions 684.35 and 480.26 m/z for Glu-fib Better than 5 ppm RMS as measured from
using nanoflow ChipMS in electrospray, peptide – 100 fmol/ul with 6 second ten 6 second spectra
300 nL/min on column accumulation (3 fmol consumed)
Mass accuracy temperature stability – Temperature: 15 to 35° C (59 to 95 °F) at Maintain 2 ppm mass accuracy within
MS mode constant temperature (variations < 3° C from 2 deg. C drift per hour
calibration temperature)
Dynamic range In-scan dynamic range on co-eluting components Up to 5 decades
Mass range m/z 25 – 20,000
Spectral acquisition rate, MS mode m/z 100 to 1700 in MS mode while maintaining 20 spectra/second
a resolution of 20,000 at m/z 1,522
Spectral acquisition rate, MS/MS mode m/z 100 to 1700 in MS/MS mode while 10 MS/MS spectra/second
maintaining a resolution of 20,000 at m/z 1,522

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General system specifications

Parameter Measure
Single point of control Single point data system method capability with full control for HPLC system and Q-TOF and
communication between the two such that if the LC or MS experiences a “not ready” condition,
error, or leak, the system stops injecting the sample.
Wide range of ionization sources • Electrospray (ESI)
• Atmospheric pressure chemical ionization (APCI)
• Atmospheric pressure photoionization (APPI)
• Multimode source (simultaneous ESI and APCI)
• HPLC-Chip/MS interface
• AP/MALDI PDF+ source based upon pulsed dynamic focusing
Transparent introduction of reference masses • Dual-sprayer orthogonal ESI source, with one sprayer for analytical flow and one for the
reference compound
• Built-in calibrant delivery system for automated introduction of reference masses
• Real-time internal reference mass correction for MS and MS/MS operation
• Internal reference mass correction for heated sources – multimode, APCI, APPI
Autotune Automated optimization of ion optics and full Q-TOF MS mass axis calibration
Rich data-dependent modes of operation • Precursor selection based upon intensity of n-highest (or n-lowest) or relative or absolute
threshold; exclude mass list and preferred mass list; dynamic selection of fragmentation
energy; preferred charge state
• Intuitive setup of data-dependent experiments
Data mining tools One-button extraction of compound-specific spectral and chromatographic information via
‘Find Compounds’ algorithms
Linked software to enable quick manual Compound-based data browsing with dynamic links to spectra and chromatograms
review of complex data sets
Software for protein identification Full compatibility with optional Spectrum Mill for MassHunter Workstation, including optimized
Data Extractor
Software for verification of recombinant
proteins or synthetic peptides
Molecular profiling tools • Measurement of potentially relevant molecular features from one or more LC/MS analyses
(MassHunter)
• Differential expression analysis to reveal features that separate one sample set from
another (optional MassHunter Mass Profiler)
• Selection of differential features for subsequent MS/MS identification
Warranty First year expanded warranty to include all labor, parts, and travel expenses. Includes preventive
maintenance and certain consumables
Safety • Canadian Standards Association
^ CAN/CSA-C22-2 No. 61010-01-4
^ CSA/Nationally Recognized Test Laboratory (NRTL) UL 61010-1:2005
• International Electrotechnical Commission (IEC) 61010-1
• EuroNorm (EN): 61010-1
Electromagnetic compatibility CISPR11/EN55011: Group 1, Class A
Sound emission EN 27779:1991 – sound pressure Lp < 70 db
Power • Americas & Japan: 200 to 220 VAC; 2500 VA max, 50/60 Hz, 15 A circuit
• Europe & Asia Pacific: 220 to 240 VAC; 2500 VA max, 50 Hz, 15 A circuit
Operating environment • Temperature: 15 to 35° C (59 to 95 °F) at constant temperature (variations < 3° C from
calibration temperature)
• Humidity: 20 to 85%
Size 48 inches long x 29 inches wide x 53 inches high
Weight 340 pounds

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Information, descriptions, and specifications in

this publication are subject to change without
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© Agilent Technologies, Inc. 2009

Printed in the USA
March 20, 2009
5990-3721EN