Cover Page
Cover Page
Cover Page
UTKAL UNIVERSITY,
BHUBANESWAR
IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE
AWARD OF DEGREE OF MASTER OF SCIENCE IN
BIOTECHNOLOGY
BY
MRUTYUNJAY DAS
Roll No:15254U204031
Under the guidance of
Dr. RASHMIRANJAN MISHRA
Associate Professor
Department of Biotechnology
Dr. S. Mohapatra
Assistant Professor
Certificate
This is to certify that the dissertation entitled “CHARACTERIZATION AND OPTIMIZATION
OF CELLULASE ENZYME FROM BACTERIA ISOLATED FROM FRUIT INDUSTRY
WASTE” submitted by Mrutyunjay Das bearing University Roll no:15254U204031 in
partial fulfillment of the requirement for the degree of Msc in Biotechnology from Utkal
university ,Bhubaneswar odisha is a faithful record of bonafide research work carried out
under the supervision of Mrs. Sonali Mohapatra, Assistant Professor, Department of
Biotechnology, CET. The results of the investigation reported in the thesis have not been
submitted for the award of any other degree.
I, Mr. Mrutyunjay Das do hereby declare that the dissertation thesis entitled “Characterization
and optimization of cellulose enzyme from bacteria isolated from fruit industry waste”
which is being submitted to Department of Biotechnology, MITS School of Biotechnology, 2(P),
Infocity, Patia, Chandaka Industrial Estate, Bhubaneswar751024, Odisha, India affiliated to
Utkal University, Bhubaneswar, Odisha, India for the Degree of Master of Science in
Biotechnology is done by me at MITS SCHOOL OF BIOTECHNOLOGY, Bhubaneswar-
751024, Odisha, India under the guidance of Dr. Sonali Mohapatra,Assistant professor, Dept.
of Biotechnology ,college of engineering and technology , Bhubaneswar, Odisha. It has not
formed in the basis for the award of any Degree/Diploma/Fellowship or other similar degree to
any candidate of any University. To reiterate, the help and support received has been duly
acknowledged.
Date:
Place:Patia, Bhubaneswar (Mrutyunjay Das)
ACKNOWLEDGEMENT
(Mrutyunjay Das)
MITS SCHOOL OF BIOTECHNOLOGY
Ref. No./
FROM Date:
DIRECTOR
This is to certify that Mrutyunjay Das is a bonafide student of M.Sc. in Biotechnology (Fourth
Semester), MITS School of Biotechnology, Bhubaneswar-24, Odisha with University Roll
No._15254U204031. he has carried out her dissertation work under the direct Supervision of
Mrs Sonali Mohapatra, Associate Professor, Dept. Biotechnology, CET, Odisha, India within
duration of six (06) months i.e. from January, 2022 to June 2022. As per my knowledge is
concerned, no part of this work has been submitted for any other degree or diploma..
Mrutyunjay Das is a fit and suitable candidate for the award of Degree of Master of Science
in Biotechnology conferred by Utkal University. She has duly acknowledged the assistance
and help received during the course of this investingation.
LIST OF TABLES
SL.NO TABLE NO PAGE.NO TITLE
1 Table 1 20 Cellulose degrading bacteria isolated from different
natural reserves
2 Table 2 35 Cellulase activities of different bacterial isolase based
on the ration between hydrolyzing zone diameter and
colony diameter
3 Table 3 37 Absorbance values of cellulase activity of 24 bacterial
isolates from fruit industry soil by DNS method after
the incubation of 24 hours
4 Table 4 40 Phenotypical characteristics of isolated cellulolytic
bacterial strains
(a)
LIST OF ABBREVATION
µl Microliter
pH Hydrogen ion concentration
ml Mililiter
M Mole
DNS Di-nitro salicylic acid
MR Methyl red
MRVP Methyl red voges-proskauer
CMC Carboxy-methyl cellulose
SCB Sub cuticular bacteria
ddH2o Double distilled water
(c)
LIST OF FIGURES
SL NO FIG NO PAGE NO TITLE
1 Fig.1 9 Single cellulose chain repeat unit showing
directionality and hydrogen bond.
2 Fig.2 13 Structure of hemicellulose
3 Fig.3 14 Chemical structure of lignin
4 Fig.4 25 Flow diagram of study design
5 Fig.5 26 Serial dilution method of soil samples
6 Fig.6 29 Reaction mixture in cellulase assay
7 Fig.7 29 Reaction mixture mixed with DNS
8 Fig.8 31 Growth of Bacterial colony on CMC agar after
incubation
9 Fig.9 32 Growth of Bacteria isolates by striking method on
agar plate.
10 Fig.10 32 Preservation of bacterial slant from SCB1-SCB34