C196-E090

High-Performance Liquid Chromatograph

Prominence Specifications
 Specifications

Solvent Delivery Units LC-20AD / 20AT / 20AB

LC-20AD (228-45000-XX) LC-20AT (228-45001-XX) LC-20AB (228-45002-XX)
Solvent delivery method Parallel-type double plunger Serial-type double plunger Parallel-type double plunger (2 sets)
Primary side: 47 μL,
Plunger capacity 10 μL 10 μL
Secondary side: 23 μL
Maximum discharge pressure 40 MPa
Flow rate setting range 0.0001 to 10.0000 mL/min 0.001 to 10.000 mL/min 0.0001 to 10.0000 mL/min
No more than ±1% or ±2 μL/min, No more than ±2% or ±2 μL/min, No more than ±1% or ±2 μL/min,
Flow rate accuracy whichever is greater whichever is greater whichever is greater
(0.01 to 2 mL/min) (0.01 to 5 mL/min) (0.01 to 2 mL/min)
Flow rate precision No more than 0.06% RSD or 0.02 min SD, whichever is greater
Gradient type High-pressure mixing/low-pressure mixing High-pressure mixing
Mixing-concentration precision 0.1% RSD max.
Constant-pressure solvent delivery Supported Not supported
Plunger rinsing mechanism Manual rinsing or automatic rinsing using optional product
Safety measures Liquid-leakage sensor, high-pressure/low-pressure limits
Operating temperature range 4 to 35°C
Dimensions, weight W260 × D420 × H140 mm, 10 kg W260 × D420 × H140 mm, 11 kg W260 × D420 × H140 mm, 13 kg
Power requirements AC 110 V, 230 V, 150 VA, 50/60 Hz AC 110 V, 230 V, 180 VA, 50/60 Hz

Inert Solvent Delivery Unit LC-20Ai

LC-20Ai (228-45293-XX)
Solvent delivery method Serial-type double plunger
Plunger capacity Primary side: 48 μL Secondary side: 24 μL
Water: 30 MPa (0.001 to 5.000 mL/min), 22 MPa (5.001 to 10.000 mL/min)
Maximam discharge pressure
Organic solvent: 22 MPa (0.001 to 10.000 mL/min)
Flow rate setting range 0.001 to 10.000 mL/min
No more than ±2% or ± 2 μL/min, whichever greater
Flow rate accuracy
(0.1 to 5 mL/min, 1.0 to 20 MPa, Water)
Flow rate precision No more than 0.06% RSD or 0.02 min SD, whichever greater
Gradient type High-pressure mixing
Constant-pressure solvent delivery Supported
Plunger rinsing mechanism Manual rinsing or automatic rinsing using optional product
Safety measures Liquid-leakage sensor, high-pressure limits, shield plate
Operating temperature range 4 to 35°C
Dimensions, weight W260 × D420 × H140 mm, 11 kg
Power requirements AC 100 V, 150 VA, 50/60 Hz

Preparative Solvent Delivery Units LC-20AR / 20AP

LC-20AR (228-45275-XX) LC-20AP (228-45150-4X)
Solvent delivery method Parallel-type double plunger
Plunger capacity 47 μL 250 μL
Maximum discharge pressure 49 MPa 42 MPa
0.01 to 100.00 mL/min (to 42 MPa)
Flow rate setting range 0.001 to 20.00 mL/min 100.01 to 150.00 mL/min (to 30 MPa)
0.01 to 50.00 mL/min (low-gradient unit)
LC-20AR
No more than ± 1% or ± 10 μL,
Flow rate accuracy ± 1% (1 mL/min, 10 MPa)
whichever is greater (0.1 to 5.0 mL/min)
Flow rate precision No more than 0.08 %RSD or 0.02 min SD, whichever is greater No more than 0.1 %RSD or 0.02 min SD, whichever is greater
Gradient type High-pressure mixing High-pressure mixing / low-pressure mixing
Constant-pressure solvent delivery Supported
Plunger rinsing mechanism Using syringe or rinsing pump (228-39625-41)
Safety measures Liquid-leakage sensor, high-pressure / low-pressure limits
Operating temperature range 4 to 35°C
Dimensions, weight W260 × D500 × H140 mm, 16 kg W260 × D500 × H210 mm, 19 kg
LC-20AP
Power requirements AC 110 V, 230 V, 150 VA, 50/60 Hz AC 110 V, 230 V, 400 VA, 50/60 Hz

2
Degassing
g g Units DGU-20A3R / 20A5R
DGU-20A3R (228-45018-XX) DGU-20A5R (228-45019-XX)
Number of degassed solvents
Nu 3 5
De
Degassed flow-line capacity 400 μL
Operating
Op temperature range 4 to 35°C
Dimensions, weight W260 × D421 × H72 mm, 3.9 kg W260 × D421 × H72 mm, 4 kg
Power requirements Supplied from LC-20AD / 20AT / 20AB / 20AR / 20AP

Versatile Autosamplers SIL-10AF / 10AP

Bio-inert Autosampler SIL-10Ai
SIL-10AF (228-45056-XX) SIL-10AP (228-45057-XX) SIL-10Ai (228-45075-XX)
Injection method Loop injection, variable injection volume
1 to 50 μL (standard)
1 to 400 μL (option) 1 to 5,000 μL (standard)
Injection-volume setting range 1 to 50 μL (standard)
1 to 2,000 μL (option) 1 to 400 μL (option)
1 to 250 μL (option)
1 to 5,000 μL (option) 1 to 2,000 μL (option)

100 with 1.5 mL vials (60 with optional cooler) 80 with 4 mL vials (50 with optional cooler)
Number of samples processed
25 with 13 mL vials (not applicable to SIL-10Ai ) 192 with two 96-well microtiter plates
Injection-volume accuracy
Inj Not specified
RSD: 0.5% max. RSD: 1% max. RSD: 0.5% max.
Inj
Injection-volume precision
(10 μL injection, standard mode) (10 μL injection) (10 μL injection, standard mode)
Sample carryover Not specified
Num
Number of repeated injections 30 max. per sample
Needle rinsing Set freely before and after sample injection
Sample cooler Optional Sample Cooler S (228-45063-XX) or L (228-45064-XX) Block-heating/cooling method 4 to 70°C
Operating pH range pH 1 to pH 10
Operating temperature range
Ope 4 to 35°C
Dimensions, weight Main unit: W260 × D420 × H280 mm, 19 kg Syringe unit: W100 × D150 × H280 mm, 4 kg
Power requirements AC 110 V, 230 V, 100 VA, 50 / 60 Hz

* SIL-10AF / 10AP / 10Ai can not control from CBM-20Alite.

Autosamplers SIL-20A / 20AC / 20AHT / 20ACHT

SIL-20A (228-45006-XX) SIL-20AHT (228-45119-XX) SIL-20AC (228-45007-XX) SIL-20ACHT (228-45120-XX)
Injection method Total-volume sample injection, variable injection volume
Maximum operating pressure 20 MPa 35 MPa 20 MPa 35 MPa
Injection-volume setting range 0.1 to 100 μL (standard), 0.1 to 2,000 μL (option)
175 (1 mL vials), 105 (1.5 mL vials), 50 (4 mL vials) 175 (1 mL vials), 70 (1.5 mL vials), 50 (4 mL vials)
Number of processed samples 192 (two 96-well MTP/DWP), 768 (two 384-well MTP/DWP) 192 (two 96-well MTP/DWP), 768 (two 384-well MTP/DWP)
Also, ten 1.5 mL vials in addition to each of the above. Also, ten 1.5 mL vials in addition to each of the above.
Injection-volume accuracy 1% max (specified conditions)
Injection-volume precision RSD: 0.3% max. (specified conditions, typically 0.2% RSD max)
Sample Carryover 0.005% max. (specified conditions, typically 0.0025% max)
Number of repeated injections
Num 30 max. per sample
Needle rinsing Set freely before and after sample injection.

Sample cooler Block cooling/heating,

None
used together with defumidifying function, 4 to 40°C
Operating pH range pH1 to pH14
Operating temperature range
Op 4 to 35°C
Dimensions, weight W260 × D500 × H415 mm, 27 kg W260 × D500 × H415 mm, 30 kg
Power requirements AC 110 V, 230 V, 100 VA, 50/60 Hz AC 110 V, 230 V, 300 VA, 50/60 Hz

* Prominence
Prom UFLC system uses SIL-20AHT UFLC version (228-45132-XX) or SIL-20ACHT UFLC version (228-45133-XX), which has outlet piping optimized for
UFLC in standard SIL-20A or SIL-20AC.

Prominence Specifications
3
 Rack Changer II
Rack Changer II (228-45164-XX)
Compatible plates 96-well MTP, 96-well DWP, 384-well MTP, 384-well DWP, 1.5 mL vial plate (54 vials)
Number of processed plates 12
Sample cooler Block cooling/heating, used together with dehumidifying function, 4 to 40°C
Operating temperature range 4 to 35°C
Dimensions, weight W425 × D500 × H415 mm, 32 kg
Power requirements AC 110 V, 230 V, 350 VA, 50/60 Hz

System Controllers CBM-20A / 20Alite

CBM-20A (228-45012-XX) CBM-20Alite (228-45011-38)
Solvent delivery units: 4 max.;
Solvent delivery units: 4 max.;
Autosamplers: 1; Column ovens: 1;
Connectable units Autosamplers (SIL-10AF/10AP/10Ai): 1;
Detectors: 2 max.; Fraction collectors: 1;
Column ovens: 1; Detectors: 2 max.
Sub-controllers: 2 max.
Number of connectable units
Nu 8 (expansion possible up to 12) 5 (including the unit incorporating the system controller)
Data buffering Approx. 24 hours for one analysis (at 500-ms sampling rate; available only with LCsolution)
Event I/O 4 inputs, 4 outputs 2 inputs, 2 outputs
Analog boards Up to 2 boards can be mounted. Mounting not supported.
CBM-20A
Operating temperature range
Op 4 to 35°C
Dimensions, weight W260 × D420 × H140 mm, 5.5 kg W120 × D100 × H20 mm, 0.5 kg
Power requirements AC 110 V, 230 V, 100 VA, 50/60 Hz Supplied from unit

Column Oven CTO-30A

CTO-30A (228-45160-XX)
Type Block heating system
Temperature
Tempe
Te setting range 4 to 150°C in 1°C steps
Temperature
Tem
mpera control precision ± 0.05°C (room temperature 25°C)
Temperature
Te
empe control range 5°C above room temperature to 150°C
Column size and capacity
Colum
C 150 mmL. × 4.6 mmI.D. column × 2
Devices
Device
es that
tha can be accommodated Gradient Mixer, High-Pressure Flow Switching Valves (2), Post Column Cooler etc.
Functions Linear temperature programs supported
SSafety measures Solvent sensor, liquid-leakage sensor, temperature fuse, temperature upper limit
Operating
Op
perati temperature range 4 to 35°C
Di
Dimensions, weight W260 × D500 × H210 mm, 10 kg
Power requirements AC 110 V, 230 V, 300 VA, 50/60 Hz

Column Ovens CTO-20A / 20AC

CTO-20A (228-45009-XX) CTO-20AC (228-45010-XX)
Temperature control method Forced-air circulation
Cooling method None Electronic cooling
Temperature setting range 4 to 85°C
Temperature control precision 0.1°C max. (typically 0.04°C max.)
Temperature control range 10°C above room temperature to 85°C 10°C below room temperature to 85°C
Storage capacity W220 × D95 × H365 mm
Storable devices 2 manual injectors, gradient mixer, 2 high-pressure flow-line selection valves, etc.
Time program Linear temperature programs supported
Safety measures Solvent sensor, temperature fuse, temperature upper limit
Operating temperature range 4 to 35°C
Dimensions, weight W260 × D420 × H415 mm, 20 kg W260 × D420 × H415 mm, 23 kg
Power requirements AC 110 V, 230 V, 600 VA, 50/60 Hz

4
Column Oven CTO-10AS VP
CTO-10ASVP (228-45059-XX)
Type Block heating
Cooling method Electronic cooling
Temperature setting range 4 to 80°C
Temperature control precision ±0.1°C
Temperature control range −15 to 60°C, room temperature
Applicable columns 25 cm (2 columns max.)
Function Change of temperature setting
Safety features Leak sensor, temperature fuse, temperature upper limit
Dimensions, weight W130 × D420 × H415 mm, 12 kg
Power requirements AC 110 V, 230 V, 120 VA, 50/60 Hz

Photodiode Array Detector SPD-M30A

SPD-M30A (228-45196-XX)
Light source Deuterium (D2) lamp
Number of diode elements
Numbe 1024
Wavelength range
Wa 190 to 700 nm
Slit width 1 nm, 8 nm
Wavelength accuracy
Wav ± 1 nm
Noise 0.4 × 10−5 AU (under specified conditions)
Drift 0.5 × 10−3 AU/h (under specified conditions)
Linearity 2.0 AU (ASTM standard)
Standard cell: Optical path length: 10 mm, Capacity: 1 μL, Pressure: 8 MPa
Cell
Optional high-sensitivity cell: Optical path length: 85 mm, Capacity: 9 μL, Pressure: 8 MPa
Functions Contour output, spectrum library, MAX plotting
Safety measures
Sa Liquid-leakage sensor
Operating temperature range 4 to 35°C
Dimensions, weight W260 × D500 × H140 mm, 12 kg
Power requirements AC 110 V, 230 V, 150 VA, 50/60 Hz

UV-VIS Detectors SPD-20A / 20AV

Photodiode Array Detector SPD-M20A
SPD-20A (228-45003-XX) SPD-20AV (228-45004-XX) SPD-M20A (228-45005-XX)
Light source Deuterium (D2) lamp Deuterium (D2) lamp, tungsten (W) lamp
Number of diode elements None 512
Wavelength range 190 to 700 nm 190 to 900 nm 190 to 800 nm
1.2 nm (high-resolution mode),
Bandwidth, slit width 8 nm 8 nm (high-sensitivity mode)
Wavelength accuracy ± 1 nm max.
Wavelength precision ± 0.1 nm max.
Noise 0.5 × 10−5 AU (under specified conditions) 0.6 × 10−5 AU (under specified conditions)
Drift −4
1 × 10 AU/h (under specified conditions) 5 × 10−4 AU/h (under specified conditions)
SPD-20A Linearity 2.5 AU (ASTM standard) 2.0 AU (ASTM standard)
Dual-wavelength detection in the range 190 to 370 nm and upwards of Contour output, spectrum library,
Functions
371 nm, ratio-chromatogram output, wavelength scanning MAX plotting
Optical wavelength: 10 mm, Optical wavelength: 10 mm,
Cell Capacity: 12 μL, Pressure: 12 MPa Capacity: 10 μL, Pressure: 12 MPa
Cell temperature control range 5°C above room temperature to 50°C
Parameter setting, log management,
Web control —
management of consumable parts, etc.
Approx. 20 minutes of data in
Buffer memory Refer to the information on the CBM-20A/20Alite the entire wavelength region
(only when using LabSolutions)
Operating temperature range
Op 4 to 35°C
SPD-M20A Dimensions, weight W260 × D420 × H140 mm, 13 kg W260 × D420 × H140 mm, 12 kg
Power requirements AC 110 V, 230 V, 160 VA, 50/60 Hz AC 110 V, 230 V, 150 VA, 50/60 Hz

* Prominence UFLC and Prominence UFLCXR system use SPD-20A UFLC version (228-45130-XX) which has a semi-micro temperature-controlled flow cell installed
for optimization of fast analysis. (Standard type temperature-controlled flow cell is optional for SPD-20A UFLC version.)

Prominence Specifications
5
 Conductivity Detector CDD-10A VP

CDD-10AVP (228-45054-XX)
Temperature coefficient 25 nS·cm−1/C (background: 285 μS·cm−1; cell temperature: 43°C)
Cell volume 0.25 μL
Cell constant 25 μS·cm−1
Material used in parts
PEEK, SUS316
making contact with liquid
Maximum operating pressure 2.9 MPa (30 kgf/cm2)
Response 0.05 to 10 sec., 10 steps
Zero adjustment Autozero, baseline shift
Operating temperature range 4 to 35°C
Dimensions, weight W260 × D420 × H140 mm, 6.0 kg
Power requirements AC110 VA, 230 V, 250 VA, 50/60 Hz

Fluorescence Detectors RF-20A / 20AXS

RF-20A (228-45147-XX) RF-20AXS (228-45148-XX)
Xenon lamp, low-pressure mercury lamp
Light source Xenon lamp
(to check wavelength accuracy)
Wavelength range 0, 200 to 650 nm 0, 200 to 750 nm
Spectral bandwidth 20 nm
Wavelength accuracy ± 2 nm
Wavelength
W reproducibility ± 0.2 nm
S/N Water Raman peak S/N 1200 min. Water Raman peak S/N 2000 min.
Cell (cap
(capacity, pressure resistance, material) 12 μL; 2 MPa (approx. 20 kgf/cm2); SUS316L, PTFE (fluororesin), quartz
Cell Temperature input range
Ce — 4 to 40°C, 1°C step
(Room temperature − 10°C) to 40°C
Cell —
(2 mL/minute max. flow rate, 85°C max. oven temperature)
Functions Any two wavelengths between 200 and 650 nm Any two wavelengths between 200 and 750 nm
Safety measures 0.5 s per wavelength
Operational temperature range 4 to 35°C
Dimension, weight W260 × D420 × H210 mm, 16 kg W260 × D420 × H210 mm, 18 kg
Power requirements AC 110 V, 230 V, 400 VA, 50/60 Hz

Refractive Index Detector RID-20A

RID-20A (228-45104-XX)
Refractive index range 1 to 1.75 RIU
Noise level 2.5 × 10−9 RIU max.
Drift 1 × 10−7 RIU/h max.
Range A mode: 0.01 × 10−6 to 500 × 10−6 RIU, P and L modes: 1 × 10−6 to 5,000 × 10−6 RIU
Response 0.05 to 10 sec, 10 steps
Polarity switching Supported
Zero adjustment Auto zero, optical zero, fine zero
Maximum operating flow rate 20 mL/min (150 mL/min with option)
Temperature control of cell unit 30 to 60°C
Cell volume 9 μL
Cell withstand pressure 2 MPa (cell unit)
Operating temperature range 4 to 35°C
Dimensions, weight W260 × D420 × H140 mm, 12 kg
Power requirements AC 110 V, 230 V, 150 VA, 50/60 Hz

6
Evaporative Light-Scattering Detector ELSD-LT ll
ELSD-LT II (228-45115-XX)
Nebulizing method Siphon Splitting
Light source LED
Detection Photomultiplier Tube
Temperature setting range Ambient to 80°C
Nebulizer gas Nitrogen (N2) or Air *1
Gas flow rate, gas pressure Max. 3.0 L/min, Max. 450 kPa
Mobile phase flow rate 0.2 to 2.5 mL/min
Analog output 0 to 1 V
Operating temperature range 5 to 40°C
Max. 80% (5 to 31°C, room temperature)
Operating humidity range
Max. 50% (31 to 40°C, room temperature)
Dimensions, weight W250 × D550 × H450 mm, 20 kg
Power supply AC 115 V, 230 V, 150 VA, 50/60 Hz

*1 Requires gas supply source, such as a gas line, nitrogen generator, or air compressor.
Note
s !N OPTIONAL PRESSURE REGULATOR WITH FILTER IS REQUIRED TO REMOVE MICROSCOPIC MATERIALS IN GAS
s 7HEN USING A NITROGEN GENERATOR OR AN AIR COMPRESSOR PLEASE BE CAREFUL THAT MOISTURE OIL DUST ETC SHOULD NOT BE CONTAINED IN NITROGEN OR AIR
s 0LEASE USE IT IN THE ROOM WHERE EXHAUST EQUIPMENT IS AVAILABLE

Fraction Collector FRC-10A

FRC-10A (228-45070-XX)
Drive system Arm-movement X–Y system
Maximum number of fractions 16 to 144 (depending on the type of rack used)
Collection method Solenoid valve (fraction-collector head with valve) or direct through nozzle (fraction-collector head)
Maximum flow rate 150 mL/min
Fraction modes Basic mode (using initial parameters), and Time-Program mode (14 different functions available)
Cooling function Possible with Sample Cooler L (228-45064-XX)
Ambient temperature range 4 to 35°C
Dimensions, weight W260 × D420 × H280 mm, 15 kg
Power requirements AC 110 V, 230 V, 100 VA, 50/60 Hz

Flow-Line Selection Valves FCV Series

FCV-12AH FCV-20AH2 / 20AH6 F

FCV-14AH
H F
FCV-13AL FCV-11AL FCV-15AL FCV-230AL
* This image is with
4 port option kit

FCV-12AH
FCV-12AH FCV 14AH
FCV-14AH FCV-11AL
FCV-32AH
FCV 32AH FCV-20AH
FCV 2
20AH2 FCV-34AH
FCV 34AH
4AH FCV-20AH
FCV 20
0AH6 FCV-13AL
FCV 13AL FCV-15AL
FCV 15AL
15A FCV-230AL
FCV 230AL
FCV-12AHi FCV-14AHi FCV-14ALS
Valve type 2-position valve, 6-port 6-position valve, 7-port 3-way solenoid valves
Solvent pH usage range 1 to10 1 to 14 1 to 10 1 to 10 1 to 14 1 to 10 2 to 12 1 to 14

34.3 MPa (12AH) 34.3 MPa (14AH)

Maximum pressure 130 MPa 34.3 MPa 100 MPa 34.3 MPa —
19.6 MPa (12AHi) 19.6 MPa (14AHi)

Dimensions (mm) W110 × D250 × H110 W110 × D270 × H150 W110 × D250× H110 W57 × D161× H44 W110 × D270 × H150 W110 × D250 × H110
Weight 4.0 kg 5.0 kg 4.0 kg 850 g 5.0 kg 4.0 kg 2.0 kg 4.0 kg 2.7 kg

* 1 An Option Box VP or a Sub-controller VP is required for control of the FCV-12AH/13AL/14AH. This does not apply to the FCV-12AH/14AH when it is connected to the CTO-20A/20AC. Two
FCV-12AH units and a total of two FCV-13AL or FCV-14AH units can be controlled from the SCL-10AVP.
* 2 When using FCV-11AL/11ALS/15AL units for solvent selection, only one of these units can be controlled from the SCL-10AVP or a solvent delivery unit. The SCL-10AVP and Option Box VP or
Sub-controller VP is required to use two of these units simultaneously.

Prominence Specifications
7
 Prominence Specifications

For Research Use Only. Not for use in diagnostic procedures.

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