MICROBIOLOGY

General microbiology
1. Contribution of Louis Pasteur to Microbiology
2. Koch's Postulates
3. Modes of transmission of infection
4. Mutations
5. Bacterial cell wall -Cell wall of gram negative bacteria diagram
Gram Positive and gram negative bacteria – difference
Bacterial flagella
Pili and flagella – difference
fimbriae – SN
Transformation and Transduction – difference
6. Endotoxin and exotoxin - difference
7. Culture media – SDA; enriched and enrichment media - difference
8. Bacterial Genetics - Genetic apparatus of bacteria;
extrachromosomal DNA (Plasmid)– significance
transposons
9.Sterilization- Physical and Chemical Methods of Sterilization
Moist heat sterilisation;
Dry and Moist heat sterilization – difference
Autoclave – SN and sterilization control
Cold Sterilization Methods
High level disinfectant
Alcohol as disinfectant
Disinfectant and antiseptic -Definition
10. Phases of bacterial growth
11. Bacterial spores

Immunology
1. Immunity – define; types; mechanism of innate immunity
-Passive and active immunity
2. Immunologic tolerance
3. Antigen – Antigenic recognition;
-Antigen presentation;
- Super antigen;
-Heterophiles antigen
-Haptens and heterophilic antigens – difference
4. IgM – Properties; IgG labelled diagram
5. Monoclonal antibody
6. Agglutination-Definition
-Principle and application
-Agglutination and Precipitation - Difference
7. T and B-lymphocytes – difference; T-cell receptor Diagram; MHC class I molecule
– diagram; Tcell dependant and Tcell independent
8. complement activation – alternate pathway; classical pathway
10. Hypersensitivity – discuss
-Type I (Anaphylaxis)
-Casoni Test
-Type II
-Difference between Type II and III
-Arthur rxn
11.immune complex hypersensitivity
12.Renginic antibodies
13.Antigenic shift and drift
14.Interferon-Viral
15.autoimmune disorders
16.Cd4/cd8 counts

Bacteriology
1. Staphylococcus – toxins and enzymes
2. Streptococcus pyogenes – virulence factors; classification
3. Toxic Shock syndrome
4. Streptococcal sore throat – sequelae; Pathogenesis
5. Rheumatic fever – Lab Diagnosis
6. Corynebacterium – characteristics; morphology; culture characteristics;
Pathogenesis of diphtheria
7. Mycobacteria – classification; Photochromogens and Scotochoromogens -
difference
8. M. hominis and bovis – difference
9. Tuberculosis – Clinical question;
-Various methods to isolate the bacteria;
-Pathogenesis & Lab Diagnosis
-Drug sensitivity testing for antibiotic resistance;
-biosafety measures required for Tb diagnosis
10. Atypical mycobacteria; pathogenesis and lab diagnosis
11. Anaerobes – classification
- methods of anaerobic culture
-Pathogenesis of Gas Gangrene
12. Naegler's Reaction
13. E. coli – diarrhoeagenic strains;
- uropathogenic E.coli
-pathogenesis
-Ecoli and klebsiella
14. Salmonella –Morphology
-colony characters and antigenic structure;
-Kaufmann-White scheme of classification;
-Pathogenesis of Enteric fever; Lab Diagnosis (Widal test)—clinical question.
15.Shigella – biochemical and antigenic classification
16. Cholera – clinical;
-toxin
-pathogenesis &lab diagnosis
17. Vibrio E1 tor – SN ;diagnosis
-V cholera and v eltor
18.Hemophilus influenzae – identification;
 -Hemophilus ducreyi
19. Mycoplasma pneumonae
20. TRIC agents
21. Rickettsia disease – classify and Lab Diagnosis
22. Gastroenteritis -causes enumerate
-lab diagnosis
23.Enterobacteriaceace family -general characters & classification
24.Haemophilia ducreyi
25. Toxins of clostridium welchi

Virology
1. Viral Inclusion body
2. Bacteriophage - SN
3. Herpes Virus – disease caused by each and their Lab Diagnosis
4. Influenza – prophylaxis; Swine flu – SN; pandemic influenza
5. ARBO virus – enumerate and characters
6.Dengue – Lab Diagnosis;
7.Japanese B Encephalitis - SN
8. Polio – virus and vaccine
9. Hepatitis A; Hepatitis B – serological test
10. Rabies – vaccine
11. Rotavirus - vaccine
12. Prion disease
13.STD-enumerate
- Syphilis-primary syphilis
-Lab diagnosis /stage wise lab diagnosis /Standard tests
- BFP reactions (Biological false positive reaction)

14. HIV -morphology

-pathology
-lab diagnosis
-opportunistic infections during HIV
15 ELISA
16.Epstein Barr virus
17.Human oncogenic virus and clinical importance
18. Haemorrhagic fever of viral origin
19.Cytomegalovirus
20.Virion
21.Slow growing viruses

Parasitology
1.Amoebiasis – Entamoeba histolytica- morphology,pathogenesis & Lab diagnosis;
-Extra intestinal amoebiasis
-Primary Amoebic Meninoencephalitis
2.Zoonotic diseases-definition
- Classifications ; bacterial zoonosis
-Significance
-Mechanism of transformation
-Mode of transmission
 -Examples
3.Blood parasites-Name
- P.vivax and p.falciparam(life cycle)
-(P.vivax)Malaria – life cycle
-lab diagnosis of malaria
-Cerebral malaria
4. Cestodes – classification;
5. Taenia soleum - life cycle;
-pathogenesis
-lab diagnosis
-clinical manifestation
- T.solium and T.suginata-difference

6. Cysticercosis
7. Nematodes – enumerate tissue and intestinal nematodes
8. Ancylostoma duodenale – life cycle;
-pathogenesis
-Lab Diagnosis
9. Filaria – life cycle
-Lab Diagnosis
-Occult Filariasis and microfilaria
10. Larva migrans
12. Loeffler's syndrome
13. Common parasitic infections in children
14 Parasites causing anemia
15.Acute encephalitis syndrome
16.Histoplasmosis capsulatum
17.Enumerate various haemoflagelates causing humans infections and life cycle of L.denovani
18.Fish tape worm
19.Kalazar- lab diagnosis
20.Protozoa cause diarrhoea -life cycle and lab diagnosis
21.Echinococcus granulusus- life cycle
22.W.bancrofti- life cycle and lab diagnosis
23.Parasitic infections of CNS and their lab diagnosis
24.Intestinal protozoal infections in man
25.Immunological mechanism In parasite infections
26. Toxoplasmic gondi-SN
-Toxoplasma infections in man
27.Dracunulous medinesis
28. Cryptosporidium

Mycology
1 Classification – clinical and taxonomical; Lab Diagnosis
-Dimorphic fungi
2 Superficial mycosis
3 Dermatophytes – SN
-lesion caused
- Lab diagnosis
4.Mycetoma :-Eumycetoma;
- eumycetoma and actinomycetoma – difference
5.Opportunistic fungal infection
6.Rhinosporidiosis-SN
- Rhinosporidium seeberi

7.Candidiasis-SN; candida ablicans and non candida albicans

8. Cryptococcosis——SN
- Cryptococcus neoformans
9.Chlamydial agents and their pathogenicity
10.Various systemic fungal infections / Systemic mycosis of human origin and their lab diagnosis
11. anti-fungal agent
12.Madura foot
13.ASPERGILLOSIS IN MAN
14.Pneumocystis cariniis
15.Trypanosomiasis
16.Trichophyton mentagraphyte
17.Cryptococcus neuformans

Applied/clinical Microbiology
1. Normal flora of human skin
2. Antibiotics working on Cell Wall of bacteria
3. Antibiotic sensitivity testing – methods
4. Drug Resistance in Bacteria; drugs resistance in bacteria with reference to R-plasmid
5. Meningitis – Lab diagnosis
6. Acute encephalitis syndrome – infectious etiology
7. Pyrexia of unknown origin – define; causes;
8. Sore throat – Causes; Lab Diagnosis
9. Acute Bacterial Pneumonia – Lab Diagnosis
10. Genital ulcer – differential diagnosis; lab diagnosis;
11. UTI – causes; lab diagnosis
12. Non-Gonococcal urethritis
13. Opportunistic infections
14. Bacterial food poisoning
15. Weil felix test
16. Sabuaraud dextrose agar media
17. Dengue lab diagnosis
18. Control of poliomyelitis
19. Neglect reactions
20. Hospital acquired infections- control
21. Bird flue
22. PHAGE CYCLE AND USE OF PHAGES
23. Aldehyde Chopra test
24. Aflatoxins
25. VDRL TEST
26. PCR
27. Water borne disease ;bacteriological examination of water
28. Milk borne disease
29. HI test
30. Bacterial infections in aids
31. Cell free and fixed coagulase
32. Quelling reaction
33. Edex test