General Biology 1: Subject Description

■ This subject is designed to enhance the

understanding of the principles and
concepts in the study of biology,
particularly life processes at the cellular
and molecular levels. It also covers the
transformation of energy in organisms.
I. CELL

a. Cell Theory f. Cell Cycle: Mitosis/Meiosis

b. Cell Structure and Functions g. Transport Mechanism

c. Prokaryotic vs. Eukaryotic Cells 1. Simple Diffusion

d. Cell Types 2. Facilitated Diffusion

e. Cell Modifications 3. Active Transport

4. Bulk/Vesicular
Transport
 CONTENT

ii. Biological molecules

• Structure and Functions of Biological Molecules

I
a. Carbohydrates
b. Lipids
/
C. e. Nucleic Acid
Proteins

dEn.zy
mes
CONTENT
1/
J.1111'
3...
'7'

-
 CONTENT

PHOTOSYNTHESIS
ii. ENERGY TRANSFORMATION

A. ATP-ADP Cycle

B. Photosynthesis

C. Cellular Respiration
 PERFORMANCE TASK

1ST Q.UARTIER
The learners shall be able to:
1. Construct a 3d MODEL of a
plant/animal/bacterial cell/ cell
membrane using a recyclable
materials.
 PERFORMANCE TASK: RUBRIC
- - c :..,..- · -. - . --. ,,.........
.
,

-
c ..........,••• a c ... - - ._ C -• ; ao•
....

_·-- .....--· ·--·-- _

lt

- ---- -....
.........,. .........

-, . _- --- --
. .. -n
......... . ... . .. ,, .. . ,:::;::.::=:·::
·---
.._

- -.-- • . . . --. .. · ·
., ,,....,.•h•
. . _.
·
... . . -
..----------- -

- -- -·--··-·--
.........
·' ---- .,.,
.,_..,. K.._,..
_
....., ....
,
tco

...,....

_ , ,....,,,..
.. -•--•••-•-o

·--,-,~.-.· - -·- - . ., . ., . ..
.
...ff°'•-· .,

c• - -·- -
. _.

·- -
• _· . - ·- - ·- - '-•,-..U.,,.-_- -
. . . .._ ..

·- -. .. -,. -
._ ._ .
. ---• - -- -

-
< ! ;" ,c - •-
-

··
--------
.
 PERFORMANCE TASK: RUBRIC
. _. ._ , - ....
· " ·--·-- -.
--·--
-,
_- - --- - - _ ---·- ------
---·
-----··.-.....
" ..

-
--
- - _.. _.,,
, . .

---!!.-.-..---..-. . -
•

-----
_..., - .,._,: , ,,
, y .. ,. .... .,. _. _.

o- < ,- ---- -- ·
.__-, .,- -, .- .. .-
---
-- · - -·- - -
· -- - -
" . '. . . ---·
- - --
--· _- -.._
,. ...
--,..... .......,, ., - ;:,;;:=-
--.---. Q_,,_ --
.,. ..
•--
_,.....,"""" ,. ._..,...... -..-
...
,Uy

...........•>-
..... "I ·-·
 -- ----..
PERFORMANCE TASK: RUBRIC

...., ....._,_........

-..-.-.-_-.-.·--·-·--.,.-·- _.
<•--u-- 4.

·-
----- .---..-
boocct.. urt•ir-,
. •. -
,

_- -.. .-. ._-

-
• - - - -

- -
· - --
, , . >
· ·
' -- . .. -.-u

_--·· -
.......
-
-
,
·......
 LESSON 1: THE CELL
(Theory, Structure and Functions)

General Blolatw 1
 OOTD: Objectives of the day!

• At the end of the lesson, I should be able to:

• Recite the postulates of cell theory and explain the roles of a cell in an
organism, particularly in the levels of organization of an organism;
• Describe the structure and functions of major and subcellular
organelles;
• Determine the role of each cellular organelle and explain how it
relates to the functions of other organelles.
 ai.fi!Ei'
,.W-•
-:.- ,A
• -'--
. • ¢
:· . .?
... .

"'-.:s
, . -
-
...-
.. -,l,ln-•::s"·
-

:.
:. : •..: = : t ! :£ :
.:
.:•

.
1: . l" ! : - ,- -..-. -, ::-

· .·.. -,- " ' .- " ' . .

. .· · ;./...__
- - 1 4 1 1 '_ . , ,
,_..,..
,:-.:i: ..
· ;.. ? -1-
.... .., .. .

". --
(',-
.::..
• ..,_><..,_. - - - · - •.,
· ·-
.-
,
, -· . ....
 His101y of cell

• In 1665, British scientist Robert I}

Hooke examined a piece of cork and /i!-.c.-•..
found little structures in it which he •
compared to cellulae.

-
• He named these structures "cells".
Later it was discovered that the cell
he had found in the cork were
actually outer walls of former plant
cell.
• In the late 1600's, Dutch scientist
Anton van Leeuwenhoek examined
different subjects , using a refined
microscope than that of Hooke.
• Leuwenhoek found moving protist F...:
and sperm, which he collectively
termed "animalcules"
 Cell Theory: Defini1ion

• The Cell Theory, developed in the mid-1800's as a result of

various discoveries about cells, it is one of the basic principles of
biology.
1. The cell is the basic unit of life;
2. All living organisms are composed of cell; and
3. New cells are created from pre-existing cell
First Postulate:Cell is the basic unit of
life

■ In order that an organism may be

considered living, it must have a cell.
■ One proof that an organism is alive
just by looking at its cell is the
movement of the organelles inside it.
 First Postulate:Cell is the basic unit of
life

• In the cork that Hooke examined, the

protoplasm of the cell had already dissipated,
indicating the cell's death.
• When Leeuwenhooek observed his teeth
scrapings, he found animalcules shooting and
spinning inside the cell.
■ In 1838, German botanist Mattthias Jacob
Schleiden established that the small
compartments in his plant specimens are
cells.
■ In 1839, a German zoologist named
Theodore Schwann after doing microscopic
studies of animal cells, instituted that all
animals are also composed of cells.
Third Postulate:
new cells are created from pre-existing cells

• German scientist Rudolf

Virchow introduced the third
tenet of the cell theory: Omnis
cellula e cellula which means
"Cells come from pre-existing
cell".
 Development of Cell Theory

• 1590 Zacharias Janssen • 1663-1665 - Robert Hooke

invented a primitive microscope viewed a thin slice of cork
with the help of his father Hans.
 Development of Cell Theory

• 1665-1676 Marcello Malpighi

and Nehemiah Grew conducted
separated investigations on plant
cell. They determined the
presence of organelles within its
cells .
 Development of Cell Theory

• 1670-1683 Anton van Leeuwenhoek upgraded

Janssen's microscope and produced his lens.
With this invention, he discovered mobile
organelles in many subjects, which he called
animalcules.
 Development of Cell Theory

• 1831- Robert Brown made a series of discoveries

about cell organelles and ultimately discovered
the nucleus. This became a major breakthrough in
the history of biology.
 Development of Cell Theory

• 1838 Matthias Schleiden microscopically examine

plants and recognized that plant parts come from
cells. In his writings in Contribution in
Phytogenesis, he proposed that the different
structures of a plant are all composed of cells.
 Development of Cell Theory

• 1839 Theodore Schwann declared that animals

are likewise composed of cells. This put an end
to the debates - whether or not plants and
animals are different in structural origin and
composition.
 Development of Cell Theory

• 1840 Albrecht von Roelliker stated that sperm and

egg are composed of cells and that all humans are
configured from cells.

• 1849 Louis Pasteur was developing fermentation, a

process to kill bacteria, he proved that bacteria
are able to multiply and that bacterial cells come
from other bacterial cells.
 Development of Cell Theory

• 1858 Rudolf Virchow declared, "Omnis cellula e

cellula" which he meant that cells come from pre
existing cells. With this conclusion, the cell theory
was completed.
Question!

• If life were to be found in other planets,

would you expect it to form cells? Why or
why not?
 ASTROBIOLOGY

• Our space exploration has been

continually searching for life in outer
space. On the space probes sent to
other planets (especially Mars), these
are equipped to look after life.
However, very high resolution are still
not possible on-site.
Cell Structure and
Functions
 MAIOR PARTS OF THE CELL

Plasma Outer covering that separates

Membrane the cell's interior from its
surrounding environment.

--
,_ --- Cutop,tncn-.

Nucleus Serves as the brain of the cell

and directs all of the cell's
activity.
N........c:lous
Plasma Membrane

• It encloses and safeguards its organelles from

possible harm that foreign materials can inflict on
them.

. . .. , . , ,
P h o , ; p hollpld
Plasma Membrane

■ It also controls the

exchange of essential
components and obtains
chemical messages from
other cells.
Plasma Membrane: FLUID MOSAIC MODEL

• The plasma membrane is a mosaic of components

primarily, phospholipids, cholesterol, and proteins-that
move freely and fluidly in the plane of the membrane.
 Components of Plasma Membrane

1. Phospholipids

■ It is a lipid made of glycerol, two fatty acid

tails, and a phosphate-linked head group.
Biological membranes usually involve two
layers of phospholipids with their tails
pointing inward, an arrangement called
a phospholipid bilayer.
 Components of Plasma Membrane

2. Cholesterol

■ It is another lipid composed of four fused carbon rings, is found

alongside phospholipids in the core of the membrane.
 Components of Plasma Membrane

3. Proteins
• Proteins help move large molecules or aid in cell recognition
• Peripheral proteins are attached on the surface (inner or outer)
• Integral proteins are embedded completely through the
membrane
CYTOPLASM

■ It is the entire region of a cell between plasma

membrane and nuclear envelope.
■ It is composed primarily of water, proteins and salts.

- -
 CYTOPLASM

■ In the cytoplasm, the organelles are

suspended in a gel-like solution called cytosol,
which is composed of both organic and
inorganic compounds.
■ Cytosol accounts for about 70% of cellular
content. It contains a rich supply of
macromolecules and smaller organic
molecules.
 CYTOPLASM

• The Cytoplasm also contains enzymes that

break down waste and enable metabolic
reactions.
• It allows for cellular expansion and growth.

• Together with cytoskeleton, cytoplasm

determines cell shape and accommodates
movement for some cell types.
Discovered by Robert Brown in 1833.
• It is the most vital part of the cell and
dubbed as the "control center"
• It directs all of the cell's activities and
determines how a cell should appear
and function.
 -
■A double layer of nuclear membrane
encloses the nucleus to keep it distinct from ., ..,
other cellular components.
■ Nucleolus serves as the site of ribosomes-
synthesis.
■ Nucleus holds chromosomes which carry
tightly wrapped and coined DNA.
The cell organelles: endomembrane
system

• The endomembrane system is a

group of membranes and
organelles in eukaryotic cells that
works together to modify, package,
and transport lipids and proteins.
 The cell organelles: endomembrane
system

• This system is made up of organelles that are communally linked

by structure specific to each of them.
• These organelles are:
1. Endoplasmic Reticulum 5. lysosomes
2. Ribosomes 6. Peroxisomes
3. Vacuoles 7. Centrosomes
4. Golgi Apparatus
 Endoplasmic rcriculum

:iiiii"'.:=:;;;; --.

-:
- - --
{

Smooth B$mic
reticulum Rough endoplasmic
rotfcurum
Endoplasmic rcriculum

• The Endoplasmic Reticulum (ER) is a series of

interconnected membranous sacs and tubules that
collectively modifies proteins and synthesized lipids.
• These two functions are performs in separate areas of
ER; the rough endoplasmic reticulum and smooth
endoplasmic reticulum.
 Endoplasmic rericulum

• The hollow portion of ER tubules is

Endoplasmic Rerlculum
called lumen or cisternal space.
• The membrane of the ER, which is
--­
a phospholipid bilayer embedded
with proteins , is continuous with
nuclear envelope.
 Rough endoplasmic reticulum

• It has ribosomes on its surface.

• It makes membrane proteins and proteins for
export out of cell.
• Proteins are made by ribosomes on ER surface
• They are then threaded into the interior of Figure 1
RibffOfflea
the Rough ER to be modified and
transported
 smooth endoplasmic reticulum

.., _
Smooth Endoplasmic Reticulum • It incorporates proteins into cisternae
and transports synthesized proteins
across the cytoplasm, thereby allowing
the synthesis of fatty acids and
phospholipids.
• It is abundant in liver cell, where it
detoxifies hydrophobic chemicals. This
makes them water-soluble for excretion.
 RIBOSOMES

Ribosome
Large
subunit

J1
Small
subunitV
RIBOSOMES

■ Ribosomes are involved in providing a frame for

proteins synthesis; hence they are site of protein
production.
• They are present in both prokaryotic and eukaryotic
cells.
■ Developing muscle cells, skin and hair cells contain
large numbers of free ribosomes.
Vacuoles

vacuole

tofMa

-? , 3,
Pllw:.odQuuta
 Vacuoles

• A vacuole is a fluid -filled vesicles enclosed by a membrane.

• It has a selectively membrane freely allowing water passage but
retaining smaller molecules within it and stores chemicals within
the cell.
• Vacuoles ability to break down large molecules makes it
comparable with lysosomes in animal cells. Likewise, both
organelles thrive in acidic environment.
 lysosomes

Hydl"Ol:ytic
nz:yanC!!
lVJ:embr.>• an.ix-ti, re

c-r:..n.spo rt _,
prorei.ns

L:,l' ososne
 lysosomes

• The lysosomes serve as digestion slots for cellular materials

that are due for expiration or are no longer useful.

• It is the cell's reprocessing area, where it hacks chemical

bonds of any foreign substance it comes contact with, in
order to recycle with the raw material
 lysosomes

• It is dubbed as the cell's "suicide bags"

• These organelles are capable for self-destruction In order
to save the rest of the other organelles from being
poisoned.
• This happens through autophagy, or the natural process of
organelle destruction.
LVSOSOMES

•Cells take in food by

phagocytosis

• Lysosomes digest the food

& get rid of wastes

• Example: macrophages
 Plasma
Membrane....._,,

Figure Urate
OxJdase
'I CrystaDine
Core
peroxisomeS

• It is a small, round organelles enclosed by single

membrane, somehow resembles that of a lysosomes.
• It is responsible for self-damage and mostly
disintegrate proteins, accommodate the breakdown of
fatty acids and detoxify many poisons that enter the
body.
• It also shields the cell from serious damage caused
reactive oxygen species (ROS) molecules
 Golgi APPARATUS

secretory
trans face vesicle

cisternae -

lumen --
incoming
transport
cBuzzle com vesicle
 Golgi APPARATUS

• Stacks of flattened sacs

• Have a shipping side (cis face) & a
receiving side (trans face)
• Receive proteins made by ER
• Transport vesicles with modified
proteins pinch off the ends

Transport
Golgi APPARATUS
vesicle
• a stack of pancakes

■ Modify, sort, & package molecules from ER

■ for storage OR

transport out of cell ••••••••

{.O...................... o....:
 CENTROSOME

Distal
appendages
I
M·oLhcr
c.enc.riolc

Dnughtcr
ccnlriole

Int.:rconnccting/
fibers
I
 CENTROSOME

• It comprised of two centrioles or cylindrical structures with

a central cavity surrounded by microtubules arranged side
by side.
• These structure take part in the assembly of spindle fibers
at cell division and contribute to the successful outcome
of mitosis.
 CENTROSOME

• It is found in all eukaryotic cells, assist in arranging

microtubules (hollow tubes of proteins) to be utilized for
cell division.
• They also enable the said microtubules to form part of the
cell's microtubules to form part of the cell's cytoskeleton.
• In this way, cellular shape is maintain and the cell structure
is stabilized.
mitochondriA

lntermembrone
n"'IO,,..ix spoce rlbOSOn"\O
DNA

cristoe
 mitochondria

... . . . . ...
O I Y t •M
■ Mitochondria are the sites of

-- - - '-"Y•diil• cellular respiration, the

metabolic process that uses
oxygen to drive the
generation of ATP by
extracting energy from
........ sugars, fats, and other fuels.
Mr! c,,od.K•AtPVSlnOOitfQ«\
end .,.gySIOf.clrl loodIQll.,ic:os•.
oc:ldand lotlyoddJ,
ADENOSINE TRIPHOSPHATE

• It is the major energy currency of the

scme cell that provides the energy for most of
energy the energy-consuming activities of the
cell.
• Mechanism: When the third phosphate
group of ATP is removed by hydrolysis,
ilt blt
o1erie,gy a substantial amount of free energy is
released.
 Phospate removed
Phospate added
Adenoslne
\, trlphosphate

Energy
Energy from released tor
breakdown celllunctlons
of molecules
• The mitochondria are oval-shaped
organelles found in most eukaryotic cells.
They are considered to be the
'powerhouses' of the cell.
• Mitochondria are most plentiful in cells
that require significant amounts of
energy to function, such as liver and
muscle cells.
 MITOCHONDRIA

• The mitochondria has two membranes that are similar in composition to

the cell membrane:
• Outer membrane-is a selectively permeable membrane that
surrounds the mitochondria.
• It is the site of attachment for the respiratory assembly of the electron
transport chain and ATP Synthase.
• It has integral proteins and pores for transporting molecules just like the
cell membrane
 MITOCHONDRIA

• Inner membrane-folds inward (called cristae) to increase surfaces for

cellular metabolism.
• It contains ribosomes and the DNA of the mitochondria. The inner
membrane creates two enclosed spaces within the mitochondria:
• intermembrane space between the outer membrane and the inner
membrane; and
• matrix that is enclosed within the inner membrane.
 CHLOROPLAST

ovgh Endoplo<mlc Retlcv'vm

IMer
Membrane,

Nuclevs
Ribosomes-f

Vocvote Slroma

lntttm-embtlnt
Space

Gnnum
CelWOII (S.:aek Of Thylakold.t)
 CHLOROPLAST

• Chloroplasts, which are found in plants and algae, are

the sites of photosynthesis.
•This process converts solar energy to chemical energy by
absorbing sunlight and using it to drive the synthesis of
organic compounds such as sugars from carbon dioxide
and water.
CHLOROPLAST

•Chloroplasts are double membrane-bound

organelles and are the sites of photosynthesis.
• The chloroplast has a system of three membranes:
the outer membrane, the inner membrane, and
the thylakoid system.
 Strunure of chloroplast

■ Outer membrane-This is a semi-porous

membrane and is permeable to small
molecules and ions which diffuse easily.
■ The outer membrane is not permeable to
larger proteins. .....
 Structure of chloroplast

■ lntermembrane Space-This is usually a

thin intermembrane space about 10-20
w. - .-,- ....
nanometers and is present between the
outer and the inner membrane of the
chloroplast.
 Strunure of chloroplast

■ Inner membrane-The inner membrane of

the chloroplast forms a border to the
stroma.
■ It regulates passage of materials in and out
of the chloroplast. In addition to the
regulation activity, fatty acids, and are
synthesized in the inner chloroplast
membrane.
 Strunure of chloroplast

■ Stroma-This is an alkaline, aqueous fluid

that is protein-rich and is present within the
inner membrane of the chloroplast.
■ It is the space outside the thylakoid space.
■ The chloroplast DNA, chloroplast ribosomes,
thylakoid system, starch granules, and other
proteins are found floating around the
stroma.
 Strunure of chloroplast

• Thylakoid System

• The thylakoid system is suspended in

the stroma. It is a collection of
membranous sacks called thylakoids.
■ Thylakoids are small sacks that
are interconnected.
 Strunure of chloroplast

■ The membranes of these thylakoids are

the sites for the light reactions of the
photosynthesis to take place. The
chlorophyll is found in the thylakoids.
■ The thylakoids are arranged in stacks
known as grana. Each granum contains
around 10- 20 thylakoids.
Practice: HOMEWORK

• Draw and label each part of plant and animal cell in a

short bond paper.
ENRICHMENT

• Why cells are generally small in size?