02 - Chapter 2 Cells 20242025
02 - Chapter 2 Cells 20242025
02 - Chapter 2 Cells 20242025
CELLS
BIO091
Semester I
2024/2025
1
SUBTOPICS
2.1 : Cell Theory
2.2 Compare and contrast the structures of prokaryotic and eukaryotic cells.
Prokaryotes Eukaryotes
Unicellular Multicellular
A) PROKARYOTES
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B) EUKARYOTES
B) EUKARYOTES
Two categories :
i) Unicellular eukaryote
• Microscopic
• Example: protist (protozoa), some algae and fungi
• Nucleus and other organelles surrounded by membrane
ii) Multicellular eukaryote: Plant cell
ii) Multicellular eukaryote: Animal cell
Differences Between Animal Cells & Plant Cells
Contain glycogen granules for carbohydrate Contain starch granules for carbohydrate
storage. storage.
• Chromosomes
• Nucleolus (plu. Nucleoli) – Contain DNA molecule.
– Each chromosome is a single DNA
– Not enclosed by a membrane.
molecule with many globular proteins called
– Site for ribosomal RNA (rRNA) histones.
synthesis, from instruction in – DNA coiled around the proteins forming
the DNA. chromatin.
– Chromatin condenses forming
chromosomes as a cell prepares to divide.
NUCLEUS
RIBOSOMES
i. free ribosomes
✔ suspended in the cytosol;
✔ produces proteins that function within the
cytosol.
● The endomembrane system regulates protein traffic and performs metabolic functions
in eukaryotic cells.
☑ Detoxification of poisons.
☑ Membranes of this system are related either through direct physical continuity or
by the transfer of vesicles (sacs made of membrane).
ENDOMEMBRANE SYSTEM :
ENDOPLASMIC RETICULUM
○ Extensive network of membranes.
○ Accounts for more than half of the total membranes in many eukaryotic cells.
● Cisternal space
○ The internal lumen/space of the ER that is separated from the cytosol by the ER membrane.
● The ER membrane,
○ is a single-layer membrane,
○ is continuous with the outer nuclear membrane.
○ separates the ER lumen/cisternal space from the cytosol.
There are two distinct regions of ER:
1. Smooth Endoplasmic Reticulum
(smooth ER)
⮚ Lacks ribosomes at the outer surface.
• System of interconnected
tubules.
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3. ORGANELLES
ENDOMEMBRANE SYSTEM :
ROUGH ENDOPLASMIC RETICULUM
• Have cisternae, studded with ribosome.
5. Proteins further modified during transit from cis to trans region in Golgi.
7. Transport vesicle that departs from the trans face contains secreted products
inside, may :
i. fuse with the plasma membrane & release content from the cell.
ii. give rise to specialized vesicles (e.g.: lysosome) or vacuoles.
iii. fuse with less mature Golgi cisternae.
iv. carry proteins back to ER (required in RER)
• Lysosomal enzymes;
☑ can hydrolyze proteins, fats, polysaccharides, and nucleic acids.
☑ Work best in an acidic environment (pH 5).
• If the lysosome breaks, the released enzymes are not effective, as cytosol has a neutral pH.
• However lysosomal membrane becomes fragile when the cell is injured, lacks oxygen, and
when an excessive amount of vitamin A present causes the lysosome to rupture and leads
to autolysis.
3. ORGANELLES
Nucleus 1 µm
Lysosome carry out intracellular digestion: ENDOMEMBRANE SYSTEM
(a) Phagocytosis
• A process by engulfing food particles.
• Food vacuole formed and fused with
lysosome.
• The enzyme digests the food.
Lysosome
Digestive
enzymes
Lysosome
Plasma
membrane
Digestion
Food vacuole
(a) Phagocytosis
Vesicle containing 1 µm
(b) Autophagy two damaged organelles
Mitochondrion Digestion
Vesicle
(b) Autophagy 38
ENDOMEMBRANE SYSTEM : 3. ORGANELLES
VACUOLES
• Vacuoles are large, single membrane-enclosed sacs.
Types of vacuoles:
1. Food vacuoles are formed by phagocytosis.
2. Contractile vacuoles
1. found in many freshwater protists, to pump excess water out of cells
(osmoregulatory function).
3. Central vacuoles
• found in many mature plant cells, hold organic compounds and water.
• Enclosed by a membrane called tonoplast.
food
vacuole Central vacuole
Cytosol
Tonoplast
cilia
nucleus
Nucleus Central
Contractile vacuole vacuole
Cell wall
(a) Food vacuole and contractile vacuole in
Paramecium Chloroplast
5 µm
Relationship among organelles of the
1. Nuclear envelope is connected to endomembrane system
rough ER. Rough ER is continuous
with smooth ER.
Intermembrane space
• Some steps of cellular Outer
membrane
respiration occurred in the
mitochondrial matrix.
Free
CELLS
ribosomes
• Cristae present a in the
mitochondrial Inner
matrix membrane
large surface area
Cristae
for enzymes that Matrix
Chapter 2
synthesize ATP.
0.1 µm
3. ORGANELLES
CHLOROPLAST
• A member of plastids.
• Plastids
Ribosomes
Stroma
Granum
1 µm
Thylakoid
OXIDATIVE ORGANELLES 3. ORGANELLES
PEROXISOME
PEROXISOMES
• Specialized metabolic compartments bounded by a single membrane.
• Peroxisomes are numerous in the kidneys and liver, which are active in detoxification.
• Peroxisomes contain oxidase that transfers hydrogen from various substrates to oxygen and
produces hydrogen peroxide (H2O2), but if H2O2 escape from the peroxisomes, they might
damage other membranes in cells.
• Catalase breaks down H2O2 into water and oxygen (.: harmless).
• Oxygen is also used by peroxisomes to break down fatty acids into smaller molecules that
are transported to mitochondria and used as fuel for cellular respiration.
Chloroplast
Peroxisome
Mitochondrion
1 µm
CYTOSKELETON
• A dense network of protein fibers extending throughout
the cytoplasm.
1. Microtubules
➢ the thickest of the three components of the
cytoskeleton.
2. Microfilaments
➢ also called actin filaments,
➢ the thinnest components.
3. Intermediate filaments
⮚ have diameters in the middle range
⮚ are made from fibrous protein subunits
⮚ are more stable than microtubules &
microfilaments.
Microtubules & microfilaments:
o formed from globular
protein (beadlike) subunits
o Can be rapidly assembled
& disassembled.
Function of Cytoskeleton
• Gives cells mechanical strength, supports the cell, and maintains its shape.
• Inside the cell, vesicles travel along tracks made up of microtubules of the
cytoskeleton.
Vesicle
Microtubule
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3. ORGANELLES
CYTOSKELETON:
MICROTUBULESMICROTUBULES
3. ORGANELLES
MICROTUBULES
EXAMPLE 1:
Centrosomes and Centrioles
⮚ function in cell division.
• In many cells, microtubules grow out from a centrosome near the nucleus.
• In animal cells, the centrosome has a pair of centrioles, each with nine
triplets of microtubules arranged in a ring, (known as 9 X 3 structure)
CYTOSKELETON: MICROTUBULES
● The centrioles are duplicated before cell division & may play a
role in some types of microtubule assembly.
CELLS
linking outer
doublets central pair
(b) Cross section of
Plasma cilium ✔ Cilium has a 9 X 2 + 2
membrane
ring structure
Basal body
0.5 µm Chapter 2
(a) Longitudinal 0.1 µm
section of cilium Triplet
Muscle cell
Actin filament
arranged parallel to
one another in
CELLS
muscle tissue
Chapter 2
Myosin filament
Myosin arm
Extending
pseudopodium
c) Cytoplasmic streaming
A circular flow of cytoplasm within cells.
Occurs due to the motion of organelles attached to actin filaments via myosin-motor proteins.
3. ORGANELLES
INTERMEDIATE FILAMENTS
3. ORGANELLES
EXTRACELLULAR STRUCTURE
• Most cells synthesize and secrete materials that are external to the
plasma membrane.
• The cell wall is an extracellular structure that distinguishes plant cells from
animal cells.
• The cell wall protects the plant cell, maintains its shape, and prevents
excessive uptake of water.
• Through it, water and small solutes (and sometimes proteins and RNA)
can pass from cell to cell.
Cell walls
Interior
of cell
Interior
of cell
0.5 µm Plasmodesmata Plasma membranes
Plasmodesmata : plant cells can communicate through specialized openings in their cell walls,
called plasmodesmata, where the cytoplasm of adjoining cells are connected.
ANIMAL CELL JUNCTIONS
CELL JUNCTIONS
Consist of:
i. Tight junctions:
i. Desmosomes
(anchoring junctions)
i. Tight junctions:
✔ Areas of tight connections
between membranes of
adjacent cells.
✔ No space remains
between the cells,
preventing leakage of
extracellular fluid &
substances between cells.
ANIMAL CELL JUNCTIONS
CELL JUNCTIONS
ii. Desmosomes
(anchoring junctions)
✔ are points of attachment
between cells.
✔ Provide cytoplasmic
channels between adjacent
cells.
● Cells undergo cell differentiation and become specialized in structure and function
Basement
membrane
Pseudostratified
Cuboidal Simple columnar Simple squamous columnar
epithelium epithelium epithelium epithelium
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i) Simple Squamous Epithelium
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ii) Simple Columnar Epithelium
84
iii) Simple Cuboidal Epithelium
● Dice-shaped cells.
● Lines blood vessels ● Specialized for secretion.
and air sacs in lungs ● Found in kidney tubules and many
● Permits exchange of glands, including the thyroid gland and
materials by diffusion salivary glands.
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iv) Stratified Squamous Epithelium
86
v) Pseudostratified Columnar Epithelium
87
Glands:
Specialized Epithelial Tissue
a) Goblet cells
- unicellular exocrine glands that secrete mucus.
b) Exocrine glands
- secrete product through a duct onto the exposed
epithelial surface.
- example: sweat and salivary gland
c) Endocrine glands
- release hormones into interstitial fluid or blood.
- example: Pituitary glands
88
Unicellular glands
Cilia (goblet cells)
Basement
membrane
(a) Goblet cells.
Skin
90
Connective Tissue
Loose connective tissue
Collagenous fiber Blood
Plasma
White
blood cells
120 μm
55 μm
Elastic Red blood cells
fiber Cartilage
Fibrous connective tissue
Chondrocytes
100 μm
30 μm
Chondroitin sulfate
Nuclei Bone Adipose tissue
Central
canal Fat droplets
700 μm
150 μm
Osteon 91
Types of Connective tissue Descriptions
1) LOOSE CONNECTIVE TISSUE • The most widespread.
• Found in:
- tendons which attach muscles to bones
- ligaments which connect bones at joints.
Types of Connective tissue Descriptions
3) BONE • Mineralized connective tissue.
● Three types:
i. Cardiac muscle
ii. Smooth muscle
iii. Skeletal muscle/striated muscle
i. Cardiac Muscle
loss.
exchange.
.
Two types of vascular tissues are:
1) Xylem
Conducts water and dissolved minerals upward from roots
into the shoots.
b) Companion cells
▪ Living
▪ Has cytoplasmic connections with sieve tube
element.
▪ Function: i) Provide ATP and materials to maintain
sieve tube elements; ii) Assists in moving sugars
into and out of sieve tube element.
c) Ground Tissue System
1. Cell Shape Isodiametric cells which are oval, Circular, oval or polyhedral. Variable in shape.
spherical or polygonal in shape. Two types: Fibres and sclereids.
2. Cell wall Thin cellulosic wall Uneven thickening on primary cell wall. Have both primary and lignified thick
secondary cell wall
3. Cytoplasm Abundant Present Absent
4. Nucleus Present – Living Tissue Present – Living Tissue Absent – Dead tissue