Manual Photometric Methods For Water Analisys 82325

DINKOTEST
Manual of Photometer Methods

for Water Analysis
Photometer DINKO D-101 Digital reader AB - T% - PPM
Photometer DINKO D-100 Digital reader T% - PPM direct - Memory
Photoanalyzer DINKO D-105 Digital reader AB - T% - PPM direct

Accessible memory
Kits for quick analysis of water
1
The analysis of industrials and drinkable waters had today a great importance because the incessant increase of the consumption and
the growing demands in the control of their quality.
The Dinkotest system contributes precision, speed and the necessary simplicity for this purpose. The photometric kits for analysis of
waters and the Photometers DINKO constitutes the system DINKOTEST for the analysis of waters.
A Photometer is used to identify substances and concentrations.
Let us consider the following: The matter absorbs energy when it is in front of an energy source, just as sound or light. Due to their
different atomic structure each substance only absorbs energy among certain levels, and the energy is proportional to the wavelength ,
E = hc / λ, where
h = constant of Plank
c = speed of the light
λ = wavelength of the light
The Photometer measures the quantity of light absorbed to different wavelengths of the incident light . With the results you can trace a
graph with light absorbed in front of the wavelength to know which wavelength absorbs the investigated substance. Using this
wavelength you can determinate the concentration of the substance. When light beam impacts in a sample, a quantity is absorbed
and another transmitted.
The transmittance (T) is defined as the proportion between the transmitted intensity of the light beam (Lt) and the initial intensity of the
light beam (Lo):
T = Lt / Lo
The absorbance (A) is defined as : A = log ( 1 / T )
Therefore the Absorbancia is directly proportional to the concentration.
A=ebC
where
A = Absorbance
C = Concentration in mol /l.
b = it is the width of the sample cuvette in cm.
e = constant proportional called molar absorbance (1 / mol - cm).
This lineal relationship is known as the “Law of Beer", see fig. 2.

Therefore if we have a standard sample with an absorbance and well-known the concentration will be easy to establish the
concentration of an unknown sample of the same substance applying the law of Beer.
Absorbance
+ Standard
Origine
Concentration
Fig. 2 “ Law of Beer “
The straight line are theoretical and in the reality they spread to be curved by what becomes necessary to determine experimentally and
for each parameter their calibration curves.
The DINKO Photometer offers these calibration curves in combination with the kits of DINKO.
The reagents of the kits are presented in tablets with aluminium blister to facilitate the use, the stability, exact dosage and the biggest
possible compactness.
The test is carried out adding tablets to the sample of water and reading the absorbance or concentrations directly in the Photometer.
The available Photometers contributes different ways to obtain the looked concentration. With the Photometer D-101 the reading of
absorbance of the parameter is gotten and with the help of the calibration charts ABS – mg/L, included with each kit, the corresponding
concentration.
2
Example - Use of Calibration Charts
Range: 0- 5.0 mg / L Fe Iron 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0,03 0,11 0,18 0,26 0,33 0,41 0,48 0,56
0,1 0,64 0,71 0,79 0,86 0,94 1,02 1,11 1,20 1,29 1,39
0,2 1,48 1,57 1,66 1,75 1,84 1,94 2,03 2,11 2,20 2,28
0,3 2,37 2,45 2,54 2,62 2,71 2,79 2,88 2,97 3,06 3,17
0,4 3,27 3,38 3,48 3,58 3,69 3,79 3,90 4,00 4,11 4,22
0,5 4,32 4,43 4,54 4,65 4,75 4,86 5,00
If Photometer reading of 0,35 absorbance is obtained at wavelength of 520 nm, then the iron concentration in the sample is 2,79 mg/L.
The first column offers us the units and tenth of absorbance (0,3) and the first line the absorbancia hundredth ( 0,05). The intersection
shown in color gives us the reading of 2,79 mg/L.
The Photometer D-100 and Photoanalizer D-105 avoids the use of charts, because has them memorized and, also, improves the
selectivity of the methods, because using filters of high resolution.
The kits is manufactured in two sizes, 50 and 250 test, while the kits " Test Tube " that incorporate the necessary reagents in tubes of
16 mm of diameter, is presented in box of 25 tubes. It is the case of the kits for the test of the Chemical Oxygen Demand ( COD) and
others as the Total Phosphorous or the Total Nitrate that require a previous digestion of the sample in the same tubes of the kit.
The incubation of the tubes is carried out with the Heater D-65 with thermal block for 24 tubes or the Heater D-64D with block of 12
tubes.
The instructions are included in the boxes of the kits and in this manual you will be able to find details about the chemical method, their
traceability and associate uncertainties.
Dilutions and ranges
The analyses are carried out directly in the cuvettes, included with the photometers, provided of an 10 ml. mark that is the quantity of
sample usually employee.
To assure the accuracy of the results obtained in the tests it is very important that the cuvette stay under good conditions. The cuvette
should clean and to dry off carefully once used. The dirt can be eliminated by immersion in a weak solution of soap. All spotted or
grated cuvette should be eliminated.
Sometimes the concentration of the parameter is very high and it surpasses the range of the kit. It will be necessary to make a previous
dilution of the sample to make the test in the like conditions in those that the calibration curves were obtained.
If the measure range is surpassed in small proportion the Photometers give reading " Error " or Out of Range" . However if the range is
surpassed in big proportions false readings can be obtained, especially in the technical based on the turbidity.
Some dilution examples can avoid confusions.
If we want to carry out an extension of the range of 10 times, it will be enough to add 1ml of sample in the tube and to complete with
water distilled until the mark of 10 ml. The found concentration will multiply for 10.
Bigger dilutions would be:
100 times. Pour 1ml of sample in appraised flask of 100 ml and to complete with water distilled until the 100 ml mark. Fill the cuvette
with 10 ml of this solution. Multiply the concentration found by 100.
Equal for 1000 times with 1ml of sample in flask of 1L and to complete with water. The multiplication would be for 1000.
The dilution factors can be introduced in the Photoanalyzer D-105 and Photometer D-100 so that the apparatus indicates the final
concentration directly.
The parameters for those that we have kits are related in the following chart.
The ranges are indicated, the wavelength in which are carried out the readings, the tests number for box and the corresponding order
codes.
Other parameters can incorporate for the user in the Photoanalyzer D-105 that has space to program 140 new calibration curves, apart
from the 60 for the DINKO kits installed.
3
Name ppm Nm 50 T 250 T Name ppm Nm 50 T 250 T
Alkalinity M 0-500 580 9404 9467 Magnesium 0-100 520 9440 9487
Alkalinity P 0-500 520 9405 9468 Manganese 0-0.03 630 9441 9488
Alkalinity T 0-500 580 9403 9466 Manganese 0-5 550 9447
Aluminium 0-0.5 580 9401 9469 Molybdate 0-20 420 9442
Ammonia (N) 0-1.0 620 9408 9470 Molybdate 0-100 420 9446 9480
Ammonia Nessler 0-15 (N) 420 9002+ Nickel 0-10 520 9448
Nitrate(N) 0-20 580 9450 9491*
Bromine 0-10.0 520 9409 9472 Nitrate (N) 0-30 420 9010+
Calcium, Hardness 0-500 580 9406 9473 Nitrite(N) 0-0.5 520 9454 9492
Chlorine Dioxide 0-5.0 520 9415 9451 Nitrite(SodiumNitr) 0-1500 490 9455 9501
Chlorine DPD F&C 0-5.0 520 9412 9474 Nitrogen Total (N) 0-30 420 9012+
Chlorine 0-250 490 9413 9483 Organphosphonate 0-20 620 9414 9502
Chloride 0-50000 520 9419 9475 Ozone 0-2.0 520 9445 9449
pH 6.8-8.4 520 9417 9504
COD (25 test) 10-150 440 9429+ Phenol 0-5.0 520 9418
COD (25 test) 100-2000 580 9430+ PHMB 0-100 620 9420
COD (25 test) 1000-20000 580 9431+ Phosphate 0-4.0 620 9432 9482*
Copper,F&Comb. 0-5.0 520 9422 9476 Phosphate 0-100 490 9426 9462
Colour 10-500Pt-Co 420 9423 Phosphorous T (P) 0-12 620 9007+
Cyanuric Acid 0-200 520 9410 9465 Potassium 0-12 520 9456 9494
DEHA 0-500 ppb 550 9439 Silica 0-4.0 620 9457 9495*
Fluoride 0-1.5 580 9433 9481* Silica 0-150 420 9421 9453*
Hardness Total 0-500 580 9434 9479 Sulphate 0-200 520 9458 9496
Hydrazine 0-0.5 440 9435◊ Sulphite 0-500 580 9459 9497
Hydrogen Peroxide 0-2.0 520 9436 9485 Sulphide 0-0.5 620 9460
Hydrogen Peroxide 0-100 490 9437 9452 Surfactants anionic 0.05-4.00 620 9371
Iron 0-5.0 520 9443 9500 Turbidity 5-400 NTU 520 9444
Iron 0-10 580 9438 Zinc 0-4.0 620 9411 9499
*200 tests. + 25 test tubes The kits of 25 test-tubes, it incorporate all the necessary reagents in each tube of 16 mm. diameter. Only is
necessary to add the sample. The incubation of COD tubes is led to end up with the Heater DINKO D-65 or D-64D
◊ 30 tests
◊◊ 150 tests
* 200 tests. +25 tubes to 25 tests. (1) To be used with Nitrate Test code 1.9010.00.
The Photometer D-101 include the filters, 420, 440, 490, 520, 580, 620 and 680 nm.
The Photometer D-100 and Photoanalyzer D-105 include one filter to be chosen between the following: 415, 450, 490, 520, 577 and
630 nm
4
Photometric Standards , with certificate of analysis. Complements
Code Article
1.9982.02 Ammonia, 1ppm, 60 ml
1.9982.01 Ammonia, 15ppm, 60 ml
1.9984.00 Cationic 1000ppm, 100 ml( Al-Ca-Cu-Cr-Fe-Mg-Mn-Ni-Zn...) to indicate metal
2.4684.00 Chloride, concentrated solution 3.545g Cl-
1.9983.00 Colour 500 units. Hazen-APHA, Pt / Co, 60 ml
1.9424.00 Conditioner reagent for ammonia test in brackish water (kit 9408)
1.9971.00 DQO 50ppm, 60 ml.
1.9972.00 DQO, 100ppm, 60 ml.
1.9974.00 DQO, 500ppm, 60 ml.
1.9977.00 DQO, 1500ppm, 60 ml.
1.9978.00 DQO, 5000ppm, 60 ml.
1.9980.00 DQO, 15000ppm, 60 ml.
1.9982.09 Nitrate, 2ppm N, 60 ml
1.9982.07 Nitrate, 30ppm N, 60 ml
1.9982.08 Nitrate, 75ppm N, 60 ml
1.9982.10 Nitrite, 0.5ppm N, 60 ml.
8.0011.13 Phosphate, 200 ppm, 60 ml
2.4788.01 Potassium, concentrated solution, 1000 ppm
1.9427.01 Powder reagent for Chrom III, 50 tests
2.6939.01 Silicium, 1000 ppm, 100 ml
1.9371.01 Solvent reagent for surfactants kit, 50 tests.
1.9982.15 Sulphate, solution 200ppm SO4, 60 ml
1.9371.03 Surfactants, anionic, 4ppm, 60 ml
1.9779.00 Turbidity, concentrated , Formazine, 4000 NTU, 125 ml
DESCRIPTION OF METHODS
ALKALINITY M. Code 1.9404.00 (50t) Photometer Method 577-580 nm

Test for Alkalinity M in boiler water and other industrial waters 0 - 500 mg / l CaCO3
The alkalinity of the water is fruit of the presence of alkaline substances as hydroxides, bicarbonates, carbonates and in smaller
measure, silicates and phosphates. Quantitatively the alkalinity is the capacity of the water to react with acid until a fixed value of pH.
The obtained value will depend on the indicator pH used. Conventionally two measures of alkalinity are applied. The alkalinity M of
methyl orange and the alkalinity P of phenolphthalein.
The alkalinity is an important parameter for the industrial waters, especially for boilers or generating plants of vapour. In these cases
very alkaline waters are used to diminish the corrosion.
The test of alkalinity M and P of DINKO contribute a simple method to check both alkalinities in the range of 0-500 mg / l of CaCO3.
The alkalinities specifically due to carbonates, bicarbonates and hydroxides can be calculated starting from the obtained data.
The test DINKO uses a colorimetric method that covers the range of 0 - 500 mg / l CaCO3. The test is very appropriate to boiler and
industrial waters
METHOD
The test DINKO is based on a colorimetric method that offers considerable advantages on the traditional titrimetric methods. It consists
on a tablet that contains the exact quantity of acid and the indicator. A tablet is added to the sample of water. A range of colours takes
place from the yellow, going by the green toward the blue for the case of the alkalinity M and of the colourless one to the purple for the
alkalinity P. The colour taken place in each one of the tests indicates the alkalinity of the water and it is measured with a DINKO
Photometer
REAGENTS AND EQUIPMENT
Alkaphot M Tablet / Round cuvette 16 mm. Ø (4pcs ). Code 1.9365.00. (cuvette used in the chart)
Photometer DINKO D-101, use calibration chart. Filter 580 nm.
Photoanalyzer DINKO D-105 or D-100, select program nr. 1
PROCEDURE
1. - To filter the sample if it is necessary to obtain a clear solution.

2. - Fill the sample tube until the mark of 10 ml with sample.
3. - Add a Alkaphot M tablet. Crush and to mix.
4. - Select filter of 580 nm filter with D-101 Photometer. With D-105 Photoanalyzer and D-100 Photometer select nr.1 program
5. - Make zero with sample without tablet. Insert tube sample. Take photometer readings.
6. - Consult Alkaphot M calibration chart for Photometer D-101. Select program nr. 1, for Photometers D-100 and D-105
5
ALKALINITY RELATIONSHIPS
Starting from the results obtained in previous procedures it is possible to classify the samples in three main forms chemical present in
most of waters, such as hydroxides, carbonates and bicarbonates. In the calculated relationships they are supposed the absence of
weak forms of alkalinity and that the hydroxides and bicarbonates are incompatible in the same sample. The chemical forms of alkalinity
expressed as mg / l of CaCO3 is calculated with the following equations:
1.- If Alkalinity P = 0 2. - If Alkalinity P > 0 and M > 2P

Then, Bicarbonate = M Then, Bicarbonate = M -2P
Carbonate = 0 Carbonate = 2P
Hydroxide = 0 Hydroxide = 0
2. – If Alkalinity P > 0 and M < 2P
Then, Bicarbonate = 0
Carbonate = 2M - 2P
Hydroxide = 2P - M
Where M and P represent the values of alkalinity M and alkalinity P respectively.

Range: 0 - 500 mg / L CaCO3 Alkalinity M 580 nm
ABS 0 1 2 3 4 5 6 7 8 9
0,0 0 2 11 20 29 38
0,1 46 53 59 64 69 74 80 85 90 96
0,2 101 106 112 117 122 128 133 138 144 149
0,3 154 158 163 167 172 176 181 185 190 195
0,4 199 204 208 213 217 222 226 231 235 240
0,5 245 249 255 262 269 276 282 289 296 303
0,6 309 316 323 330 336 343 350 362 374 386
0,7 398 410 423 435 447 459 470 485 500
Note
The form in that the results of alkalinity are indicated sometimes causes confusion. The result is usually expressed as mg/l of CaCO3
(calcium carbonate). It is a form that allows the comparison with different results, and it doesn't indicate that the alkalinity is necessarily
of calcium carbonate.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 8,565 mg/L CaCO3
Traceability: The traceability of the method settles down with Standards Methods"
ALKALINITY P. Code 1.9405.00 (50t) Photometer Method 520 nm

Test for alkalinity P in boiler water and other industrials waters 0 - 500 mg / l CaCO3
The Alkalinity of water is caused by the presence of alkaline substances such as hydroxides, carbonates , bicarbonates and, to a lesser
extent, silicates and phosphates. Quantitatively alkalinity is the capacity of the water to react with acid to a specified pH end point. The
value obtained will depend on the pH indicator used. Two measures of alkalinity are conventionally applied-Alkalinity M(alkalinity to
methyl orange) and Alkalinity P(alkalinity to phenolphthalein)
Alkalinity is an important test parameter in a number of industrial water uses, notably in boiler water treatment. Boilers and steam raising
plant are normally operated under conditions of high alkalinity in order to minimise corrosion.
The Alkalinity DINKO test provide a simple means of checking alkalinity P levels over the range 0-500 mg / l of CaCO3. The alkalinities
specifically due to carbonates, bicarbonates and hydroxides can be calculated from the various data obtained.
METHOD
The DINKO Alkalinity P test is based on a colorimetric method. These method offer considerable advantages over the titrimetric
methods traditionally used.
The test is based on the use of a single tablet reagent containing a precisely standardised amount of acid with a colour indicator. The
test is carried out by adding the tablet to a sample of water. A distinctive series of colours is produced- from colourless to purple . The
colour produced is indicative of alkalinity and is measured using a DINKO Photometer

Alkaphot P Tablets / Round cuvette 16 mm Ø with cap.(4pcs ). Code: 1.9365.00. (cuvette used in the chart)
Square cuvette 10 mm with cap. (100pcs). Code: 1.9363.00
Photometer DINKO D-101, use the calibration chart.
Photoanalyzer DINKO D-105 and D-100 select program nr.2
PROCEDURE
1. – Filter sample if necessary to obtain a clear solution.
2. – Fill the test tube to the 10 ml mark with the sample.
3. – Add one Alkaphot P tablet, crush and mix to dissolve. Stand two minutes to allow colour development.
4. – Select wavelength 520 nm. on the Photometer.
5. – Make zero with the sample without tablets. Take Photometer reading immediately (see Photometer Instructions)..
6. – Consult Alkalinity P calibration chart ( D-101). Select program nr.2 (D-105 and D-100)
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ALKALINITY RELATIONS
From the results obtained from the foregoing procedures it is possible to classify the sample into the three main chemicals forms of
alkalinity present in most waters, namely hydroxides, carbonates and bicarbonates. This calculated relations assumes the absence of
other weak forms of alkalinity and also assumes that hydroxides and bicarbonates are not compatible in the same sample. The chemical
forms of alkalinity, expressed as mg / l de CaCO3 are calculated by the following equations:
1.- If Alkalinity P = 0 2. – If Alkalinity P > 0 and M > 2P
Then, Bicarbonate = M Then, Bicarbonate = M -2P
Carbonate = 0 Carbonate = 2P
Hydroxide = 0 Hydroxide = 0
3. - If Alkalinity P > 0 and M < 2P
Then, Bicarbonate = 0
Carbonate = 2M - 2P
Hydroxide = 2P - M
Where M and P are the results of the Alkalinity M and Alkalinity P test.
Range: 0 - 500 mg / L CaCO3 Alkalinity P 520 nm
ABS 0 1 2 3 4 5 6 7 8 9
0,0 6 20 35 49 64 78 93
0,1 103 110 117 124 131 137 144 151 158 165
0,2 171 178 185 192 199 205 211 218 224 230
0,3 236 243 249 255 262 268 274 281 287 293
0,4 299 304 309 314 319 324 328 333 338 343
0,5 348 352 357 362 367 372 376 381 386 391
0,6 396 401 407 413 419 425 432 438 444 450
0,7 457 463 469 475 481 488 494 500
The expression of alkalinity results sometimes causes confusion. It is normal practice to express the results as mg / l de CaCO3
(calcium carbonate). This is merely a convention to allow the comparison of different results and does not necessarily indicate that the
alkalinity is present in the water in this form. The different chemical forms of alkalinity have been referred to in the test instructions.
ALKALINITY TOTAL. Code 1.9403.00 (50t) - 1.9466.00 (250t) Photometer Method 577-580 nm
Test for total alkalinity in boiler water and other industrials waters 0- 500 mg / l CaCO3
The Alkalinity of water is caused by the presence of alkaline substances such as hydroxides, carbonates , bicarbonates and, to a lesser
extend, borates, silicates and phosphates. In water at pH neutral the alkalinity derives mainly from the presence of bicarbonates.
Alkalinity is an important test parameter in a number of industrial water uses, notably in boiler water treatment. Boilers and steam raising
plant are normally operated under conditions of high alkalinity in order to minimise corrosion; if the total alkalinity is high the water may
more readily promote scale formation.
The Alkalinity DINKO test provide a simple means of checking total alkalinity levels over the range 0-500 mg / l of CaCO3.
METHOD
The DINKO Total Alkalinity test is based on a colorimetric method. These method offer considerable advantages over the titrimetric
methods traditionally used.
The test is based on the use of a single tablet reagent containing a precisely standardised amount of a colour indicator. The test is
carried out by adding the tablet to a sample of water. A distinctive series of colours is produced- from yellow, through green, to blue. The
colour produced is indicative of alkalinity and is measured using a DINKO Photometer
Alkaphot Tablets / Round cuvette 16mm Ø with cap.(4pcs ). Code: 1.9365.00. ( cuvette used in the chart)
Square cuvette 10 mm with cap.(100pcs). Code: 1.9363.00
Photometer DINKO D-101, use the calibration chart. Filter 580nm
Photoanalyzer DINKO D-105 and D-100 select program nr.3.
PROCEDURE
1. – Fill the test tube to the 10 ml mark with the sample.
2. – Add one Alkaphot tablet, crush and mix to dissolve.
3. – Select wavelength 580nm (D-101). With Photoanalyzer D-105 and D-100 select nr. 3 program.
4. – Make zero with sample without tablets. Take Photometer reading immediately (see Photometer Instructions)..
5. – Consult Alkalinity calibration chart ( D-101). Select program nr. 3 (D-105 and D-100)
Range: 0 - 500 mg / L CaCO3 Alkalinity Total 580 nm
ABS 0 1 2 3 4 5 6 7 8 9
0,0 0 7 15 22 30 37
0,1 45 52 60 67 75 82 90 97 103 107
0,2 112 117 121 126 131 136 140 145 150 154
0,3 159 164 168 173 178 182 187 192 196 201
0,4 206 211 216 221 226 231 236 241 246 250
0,5 255 260 265 270 275 280 285 290 295 300
0,6 308 317 325 334 342 350 359 367 376 384
0,7 392 401 410 418 427 436 444 453 462 470
0,8 479 488 500
7
The expression of alkalinity results sometimes causes confusion. It is normal practice to express the results as mg / l de CaCO3
(calcium carbonate). This is merely a convention to allow the comparison of different results and does not necessarily indicate that the
alkalinity is present in the water in this form.
To convert Total Alkalinity as CaCO3 to Total Alkalinity as HCO3 - multiply result by 1,22.
ALUMINIUM. Code 1.9401.00 (50t) - 1.9469.00 (250t) Photometer Method 580-577 nm

Test for Aluminium in natural and drinking water 0-0,5 mg / l Al
Aluminium sulphate is used as a coagulant in drinking water treatment. The determination of aluminium is usually required for the
control of alum coagulation and filtration process at water works.
Aluminium salts are found in natural waters; levels are reported to increasing particularly in areas affected by acid rain. High aluminium
levels can be toxic to fish and aquatic life. Aluminium determination is necessary for environmental control and for testing water used for
fish farms, etc.
The DINKO test provides a simple method of measuring aluminium levels in natural and drinking waters over the range 0 – 0,5 mg / l.
METHOD
Aluminium reacts with eriochrome cyanine R indicator in slightly acid solution to produce a pink-red coloured complex. The presence of
ascorbic acid eliminates interference from iron and manganese. In the DINKO test the reagents are incorporated into two tablets. The
test is carried out by adding one of each tablet to a sample of water. The first tablet acidifies the sample to bring any colloidall aluminium
into solution and the second tablet buffers the solution to provide the correct conditions for the test.
The intensity of colour produced in the test is proportional to the aluminium concentration and is measured using a DINKO Photometer

Aluminium nr. 1 Tablets / Aluminium nr. 2 Tablets /Round cuvette 16 mm Ø with cap. (4pcs ). Code: 1.9365.00. ( cuvette used in the
chart)
Square cuvette 10 mm with cap. (100pcs). Code: 1.9363.00 /Photometer DINKO D-101, use the calibration chart. Filter 580 nm
Photoanalyzer DINKO D-105 and D-100 select program nr. 26.
SAMPLE COLLECTION
Aluminium is readily absorbed on to the surfaces of sample containers, particularly glass containers. To avoid loss of aluminium collect
samples in plastic bottles and test as soon as possible after collection. Sample bottles should be acid-rinsed and thoroughly washed out
with deionised water before re-use.
PROCEDURE
1. - Fill the test tube to the 10ml mark with the sample.
2. - Add one Aluminium nr. 1 tablet, crush and mix to dissolve.
3. - Add one Aluminium nr. 2 tablet, crush and mix gently to dissolve. Avoid vigorous agitation.
4. - Stand for five minutes to allow full colour development.
5. - Select wavelength 580 nm.(D-101) or select program nr. 26 with D-100 and D-105.
6. - Make zero with the sample without tablets.Take Photometer reading immediately (see Photometer Instructions)
7. - Consult Aluminium calibration chart ( D-101). Select program nr. 26 (D-105 and D-100)
INTERFERENCES
The presence of polyphosphate or fluoride can lead to low aluminium readings. Polyphosphate is unlikely to be present in significant
quantities in normal water samples. Fluoride will only be significant for control samples from waters works where fluoridation is
practised. In such cases samples should preferably be taken before the final fluoridation stage.
For samples taken after fluoridation such as those from water distribution systems, or samples containing natural fluoride, the aluminium
concentration should be corrected. To obtain the corrected aluminium concentration multiply the calibration chart value by the factor (1+
0.4 F) where F is the Fluoride concentration as mg/l F. The fluoride concentration should be determined separately by normal test
procedure.
Range: 0- 0,5 mg/L Al 580 nm
ABS 0 5 ABS. 0 5
0,06 0,00 0,01 0,13 0,23 0,24
0,07 0,03 0,04 0,14 0,26 0,27
0,08 0,06 0,08 0,15 0,29 0,31
0,09 0,09 0,11 0,16 0,33 0,35
0,10 0,12 0,14 0,17 0,38 0,40
0,11 0,16 0,18 0,18 0,45 0,50
0,12 0,19 0,21
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,043 mg/L Al
Traceability: The traceability of the method settles down with E.B. Sandell Chemical Analysisi Volume III, USA.
8
AMMONIA (Nessler).Code 1.9002.00 (25t) Photometer Method 420- 415 nm
Test for ammonia in natural sea and waste water 0- 15 mg / l N
The ammonia appears as a result of the degradation of nitrogenous material contained in the waters. It is also in the domestic effluents
and in certain waste waters of the industry.
The ammonia is noxious for the fish and other forms of aquatic life. Their level should be controlled carefully in fish farms waters and
aquariums.
The DINKO test is a simple method of measuring ammonia (ammonia nitrogen)over the range 0-15 mg/ l N.
METHOD
The DINKO test is based on the method Nessler. The reagent of Nessler (Potassium tetraiodomercurate(II)) reacts quickly with the
ammonia under alkaline conditions to form an orange-brown product. Before adding to the sample the reagent of Nessler a solution of
Rochelle salt it is added to avoid the turbidity that would take place due to the hardness.
The intensity of the produced colour is proportional to the ammonia concentration and it is measured with a Dinko Photometer.

Reaction tubes, 25 u
Nessler Reagent / Pasteur pipette or Syringe
Round cuvettes 16 mm Ø w/cap. (4pcs ). Code 1.9365.00
Photometer DINKO D-101, use the calibration chart, filter 420 nm.
Photoanalyzer DINKO D-105 and Photometer D-100, select the program nr. 58
PROCEDURE
1. - Remove the cap of the reaction tube and add 5,0 sample ml with the Pasteur pipette. Close the tube and invert three times to mix.
2. - Add 12 drops of Nessler Reagent to the reaction tube. Close and to invert several times to mix.
3. - Wait one minute so that the colour is developed.
4. - Select the 420 nm. filter on the D-101 and select program nr. 58 on the D-105 or D-100 photometers.
5. - Make the zero using a reaction tube without to use. Can be also used a tube with distilled water.
Take the Photometer reading in the usual way.
6. - Consult the calibration chart ( D-101). Select program nr. 58 in the D-105 and D-100
Notes
1. - The Nessler Reagent is toxic. Use it with caution. It will only be used professionally in the analysis of waters.
2. - The Nessler Reagent is sensitive to air. Cover it once used.
3. - The ammonia concentrations can be expressed in different ways:
To convert from N to NH4 ---- multiply by 1,3 / To convert from N to NH3---- multiply by 1,2
4. - Interferences. It has been added enough Rochelle Salt to avoid the turbidity in hardness until 1000 mg/L. The test can be used on
seawater without a pre-treatment of the sample.
RANGE: 0 - 15 mg/L N Ammonia Nitrogen mg/L N 420/415 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.08 0.20 0.32 0.43 0.55 0.67 0.78 0.90
0.1 1.02 1.13 1.25 1.37 1.49 1.61 1.73 1.84 1.96 2.08
0.2 2.20 2.32 2.44 2.55 2.67 2.79 2.91 3.03 3.14 3.26
0.3 3.38 3.50 3,62 3.74 3.85 3.97 4.09 4.21 4.33 4.45
0.4 4.55 4.61 4,67 4.73 4.79 4.84 4.90 4.96 5.02 5.08
0.5 5.14 5.20 5,25 5.31 5.37 5.43 5.49 5.55 5.60 5.66
0.6 5.72 5.78 5.84 5.90 5.96 6.01 6.07 6.13 6.19 6.25
0.7 6.32 6.52 6.73 6.93 7.13 7.33 7.54 7.74 7.94 8.14
0.8 8.35 8.55 8.75 8.96 9.16 9.36 9.56 9.77 9.97 10.2
0.9 10.4 10.6 10,8 11.0 11.2 11.4 11.6 11.8 12.00 12.2
1.0 12.4 12.5 12.6 12.8 12.9 13.0 13.1 13.2 13.3 13.5
1.1 13.6 13.7 13.8 13.9 14.0 14.2 14.3 14.4 14.5 14.6
1.2 14.8 14.9 15.0
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,297 mg/L N
Traceability: The traceability of the method settles down with “Standards Methods” SM 4500-NH3C y USEPA 350.2
AMMONIA. Code 1.9408.00 (50t) - 1.9470.00 (250t) Photometer Method 620- 630 nm
Test for Ammonia in natural, drinking and waste water 0- 1,0 mg / l N
Ammonia occurs as a breakdown product of nitrogenous material in natural waters. It is also found in domestic effluents and certain
industrial waste waters. Ammonia is harmful to fish and other forms of aquatic life, and the ammonia level must be careful controlled in
water used for fish farms and aquariums. Ammonia tests are routinely applied for pollution control on effluents and waste waters, and for
the monitoring of drinking water supplies.
The DINKO Ammonia Test provides a method of measuring ammonia over the range 0-1,0 mg / l N.
9
METHOD
The DINKO Ammonia test is based on an indophenol method. Ammonia reacts with alkaline salicylate in presence of chlorine to form a
green-blue indophenol complex. Catalyst are incorporated to ensure complete and rapid colour development. The reagents are provided
in form of two tablets for maximum convenience. The test is simply carried out by adding one of each tablet to sample of the water. The
intensity of colour produced in the test is proportional to the ammonia concentration and is measured using a DINKO Photemeter

Ammonia nr. 1 Tablet
Ammonia nr.2 Tablet
Round cuvette 16mm Ø with cap. (4pcs ). Code 1.9365.00.
Photometer DINKO D-101 use the calibration chart. Filter 620 nm
Photoanalyzer DINKO D-105 and D-100 select program nr.4.
TEST PROCEDURE
1. - Fill test tube with sample to the 10ml mark.
2. - Add one Ammonia nr. 1 and one Ammonia nr. 2 tablet, crush and mix to dissolve.
3. - Stand for ten minutes to allow colour development.
4. - Select wavelength 620 nm. on Photometer.
5. - Make zero with sample without tablets. Take Photometer reading (see Photometer instructions)..
6. - Consult Ammonia calibration chart (D-101). Select program nr. 4 (D-105 and D-100).
SEA WATERS SAMPLES

Ammonia Conditioning Reagent code 1.9424.00 is required when testing sea water or brackish water samples to prevent precipitation of
salts. The reagents is supplied in a special “spoon pack” to aid measuring out the powder.
Fill the test tube with sample to the 10 ml mark, and add two level spoonful of conditioning reagent. Mix to dissolve reagent then
continue the test as described in the above test instructions.
Notes
1. - At low temperatures the rate of colour development in the test may be slower. If the sample temperature is below 20ºC, allow 15
minutes for the colour development.
2. - Ammonia concentration can be expressed in a number of different ways. The following factors may be used for the conversion of
readings: To convert from N to NH4 multiply by 1,3. / To convert from N to NH3 multiply by 1,2.
RANGE: 0-1.0 mg / L N PPM AMMONIA N 620 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.1 0.00 0.02 0.03 0.05 0.06 0.08 0.09 0.11 0.12
0.2 0.14 0.16 0.17 0.19 0.20 0.21 0.23 0.24 0.26 0.27
0.3 0.28 0.30 0.31 0.32 0.34 0.35 0.36 0.38 0.39 0.40
0.4 0.42 0.43 0.44 0.45 0.46 0.48 0.49 0.50 0.51 0.52
0.5 0.54 0.55 0.56 0.57 0.58 0.60 0.61 0.62 0.63 0.64
0.6 0.65 0.66 0.67 0.68 0.69 0.70 0.71 0.72 0.73 0.74
0.7 0.75 0.76 0.77 0.78 0.79 0.80 0.81 0.83 0.84 0.85
0.8 0.87 0.88 0.89 0.91 0.92 0.93 0.94 0.96 0.97 0.98
0.9 1.00
Traceability: The traceability of the method settles down with Cli. Chim. Acta., 14 403 (1966)
BROMINE. Code 1.9409.00 (50t) - 1.9472.00(250t) Photometer Method 520nm

Test for free, combined and total bromine in water 0- 10,0 mg / l
Bromine and bromine-release compounds are used for the disinfection of swimming pool water, and many other water treatment
systems. The measurement of the bromine residual is essential for the control of these processes.
The bromine concentration can be expressed in terms of free bromine, combined bromine or total bromine. However free and combined
bromine are both considered powerful disinfectants and it is not normally necessary to differentiate between these two forms. For the
majority of applications therefore the measurement of total residual is sufficient. DPD Bromine method is a simple means of measuring
bromine residuals over the range 0 - 10.0 mg/l.
METHOD
The DINKO bromine test uses the DPD method developed by Dr. A T Palin now internationally recognised as the standard method of
testing for disinfectant residuals. In the DPD method the reagents are provided in tablet form for maximum convenience and simplicity of
use.
Bromine reacts with diethyl-p-phenylenediamine (DPD) in buffered solution to produce a pink coloration. The intensity of colour is
proportional to the total bromine concentration and is measured using a DINKO Photometer.
For the separate determination of free and combined bromine, a supplementary procedure using sodium nitrite is used. The nitrite
destroys the free bromine in the sample and the colour produced in the DPD test then corresponds to the combined bromine only. The
free bromine content is thus obtained by difference between the total bromine and combined bromine results.

DPD Nº 1 Clear Tablet / DPD Nitrite Tablet / Round cuvette 16 mm Ø with cap. (4pcs ). Code: 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use calibration chart.
DINKO D-105 Photoanalyzer and D-100 Photometer select program nr. 28.
10
PROCEDURE
1. - Rinse test tube with sample leaving two to three drops of sample in the tube.
2. - Add one DPD nr.1 tablet, crush tablet and then fill the tube test with sample to the 10ml mark.
3. - Select wavelength 520 nm on the Photometer.
4. - Take Photometer reading (see Photometer instructions). Make zero with the sample without tablets.
5. - Consult Bromine calibration chart. The value corresponding to the observed absorbance reading represents the Total
Bromine residual as milligrams per litre. For most purposes the test can be terminated at this stage.
If it is desired to measure free and combined bromine proceed as indicated in the following section.
TEST PROCEDURE-FREE AND COMBINED BROMINE

1. - Fill test tube with sample to the 10 ml mark. Add one DPD Nitrite tablet, crush and mix to dissolve.
2. - Take a second clean test tube and add two to three drops of solution from the first tube. Add one DPD nr. 1 tablet, crush and then
add the remainder of the solution to make up to the 10 ml mark. Mix to dissolve tablet.
3. - Select wavelength 520 nm on Photometer.
4. - Take Photometer reading. Consult Bromine calibration chart (D-101). Select program nr. 28 (D-105 and D-100)
5 .- The value corresponding to the observed absorbance reading represents the combined bromine residual as mg/l.
6. - The free bromine residual is obtained by subtracting the combined bromine residual result from the total bromine residual result.
Free Bromine = Total Bromine - Combined Bromine
Range: 0-10,0 mg / L Bromine 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.07 0.15 0.23 0.31 0.39 0.47 0.54 0.62 0.70
0.1 0.78 0.86 0.93 1.01 1.09 1.17 1.25 1.32 1.40 1.48
0.2 1.56 1.63 1.71 1.79 1.87 1.95 2.03 2.12 2.21 2.31
0.3 2.40 2.50 2.59 2.68 2.78 2.87 2.96 3.06 3.15 3.24
0.4 3.34 3.43 3.52 3.62 3.71 3.80 3.90 3.99 4.08 4.16
0.5 4.25 4.34 4.42 4.51 4.59 4.68 4.77 4.85 4.94 5.03
0.6 5.11 5.20 5.28 5.37 5.46 5.54 5.63 5.72 5.80 5.89
0.7 5.97 6.13 6.31 6.49 6.67 6.85 7.03 7.21 7.39 7.57
0.8 7.75 7.93 8.05 8.12 8.20 8.28 8.35 8.43 8.51 8.58
0.9 8.66 8.74 8.81 8.89 8.97 9.04 9.12 9.20 9.27 9.35
1.0 9.43 9.50 9.58 9.66 9.73 9.81 9.89 10.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0.128 mg/L Br
Traceability: The traceability of the method settles down with “Standards Methods” SM
CALCIUM, HARDNESS. Code 1.9406.00 (50t) -1.9473.00 (250t) Photometer Method 577-580nm
Test for Calcium Hardness in natural and treated water 0-500 mg / l CaCO3
Calcium hardness is caused by the presence of Calcium ions in the water. Calcium salts can be readily precipitated from water and high
levels of calcium hardness tend to promote scale formation in water systems. Calcium hardness is an important control test in industrial
water systems such as boilers and steam raising plants and for swimming pool waters.
METHOD
The Calcium Hardness test is based on the orange coloration produced by Calcicol indicator reagent with calcium ions in alkaline
solution method. The reagent itself gives a violet colour in solution. Thus at different Calcium levels a distinctive range of colours from
violet to orange is produced.
The reagents for the method are provided in form of two tablets. The test is carried out simply by adding one of each tablets to a sample
of the water. The colour produced is indicative of the Calcium hardness and is measured using a DINKO Photometer.

Calcicol nº 1Tablet / Calcicol nr. 2 Tablet / Round cuvette 16 mm Ø with cap. (4pcs ). Code 1.9365.00. (cuvette used in the chart)
Square cuvette 10 mm with cap.(100pcs). Code 1.9363.00
Photometer DINKO D-101, use calibration chart. Filter 580 nm.
Photoanalyzer DINKO D-105 and D-100 select program nr, 5
PROCEDURE
1. - Filter sample if necessary to obtain a clear solution.
2. - Fill the test tube with sample to the 10ml mark.
3. - Add one Calcicol nr. 1 tablet, crush and mix to dissolve.
4. - Add one Calcicol nr. 2 tablet, crush and mix to dissolve.
5. - Stand for two minutes to allow full colour development.
6. - Select wavelength 580 nm on the Photometer D-101.Select program nr.5 with D-100 and D-105 Photometers
7. - Take Photometer reading (see Photometer instructions). Make zero with the sample without tablets.
8. - Consult Calcicol calibration chart(D-101). Select program nr. 5 ( D-105 and D-100) .
INTERFERENCES
1. - Magnesium hardness(up 200 mg / l as CaCO3 ) does not interfere with the test.
2. - Iron at levels above 10 mg / l may cause low results. Zinc above 1 mg / l may cause high results.
3. -The pH required in the test is closely controlled by buffer mixture included in the tablet formulation.
However, avoid exceeding the buffer capacity, strongly acid or alkaline samples should be adjusted to the pH range 4 to 10,
before start test.
11
Notes
1. - The expression of hardness results sometimes causes confusion. It is normal practice to express the results of hardness test as
mg/l de CaCO3 (Calcium carbonate). This is merely a convention to allow the comparison of different results and does not indicate
that the hardness is present in the water in this form.
Results may also be expressed as mg/l Ca. To convert mg/l CaCO3 to mg/l Ca multiply by 0,4
2. - Some relations of interest are the following: 1º dH = 1,25º eH = 1,8 fH = 17,8 mg / l CaCO3
where dH = german hardness ; eH = english hardness ; fH = french hardness.

Water below 90mg in Calcium carbonate have a light hardness, between 90 and 180 normal hardness, between 180 and 270 mg
moderate hardness, and between 270 and 500 mg high hardness.
3. -The Magnesium hardness can be determinate using Magnesium kit or by taking the difference between the Total Hardness kit and
Calcium Hardness kits results.
RANGE: 0- 500 mg/L Ca CO3 Calcium, Hardness 580 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.1 500 464 423 382
0.2 343 311 278 246 213 193 181 170 158 147
0.3 135 123 112 100 95 89 84 78 73 68
0.4 62 57 52 48 45 42 40 37 34 31
0.5 28 26 23 20 17 15 12 9 6 3
0.6 0
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 5,671 mg/L CaCO3 for values below 100
mg/L and ± 20,09 mg/L CaCO3 for values up to 100 mg/L
Traceability: The traceability of the method of settles down with “Standards Methods” SM
CHLORIDE. Code 1.9419.00(50t) - 1.9475.00 (250t) Photometer Method 520 nm

Test for Chloride salt in water. 0-50 mg/l Cl to 0-50,000 mg/NaCl
The DINKO Chloride test provides a simple method to measuring chloride salt levels. There are many applications in water technology
that require determination of chlorides. These include the measurement of low levels of chloride to determinate the extent of carry-over
in boiler condensates; chloride determination to assess salt build-up in swimming pools or boiler water; and measurement of high
chlorides levels for testing sea water or determining the saltiness of brackish waters. A further application is for checking swimming
pools where salt has been artificially added to simulate sea water bathing, or where this is necessary for the operation of certain types of
electrolytic hypochlorite generator.
The test can be used for measuring these widely different chloride concentrations by varying the sample size selected. These
ranges covered are: 0 - 50 mg/l Cl, 0 - 500 mg / l Cl, 0 - 10,000 mg/l NaCl, 0 - 50,000 mg / l NaCl.
METHOD
The Chloride test is based on a tablet reagent containing silver nitrate. Chlorides react with the silver nitrate to produce insoluble silver
chloride. At the chloride levels encountered in the test, the insoluble silver chloride is observed as turbidity in the test sample. The
degree of turbidity is proportional to the chloride concentration and is measured using a DINKO Photometer.
The test is carried out under acidic and oxidising conditions so as to prevent interference from complexing agents such as EDTA and
polyphosphates, and from any reducing substances which may be present in the water. Polyacrylates do however interfere and the test
should not be used on industrial waters using polyacrylate-based treatments.

Acidifying CD Tablets/ Chloridol Tablets/ Measuring Syringe 1 ml
Round cuvette 16 mm Ø with cap. (4pcs ). Code 1.9365.00. (cuvette used in the chart)
Square cuvette 10 mm with cap. (100pcs). Code 1.9363.00 / DINKO D-101 Photometer use calibration chart.
DINKO D-105 Photoanalyzer and D-100 select program 7-54-55 or 56 according selected range.
PROCEDURE
For Testing Boiler Condensate and Softened Waters
Range 0 -50 mg / l Cl.
Fil test tube with sample to the 10 ml mark.
For Testing Natural Waters, Swimming Pools and Boiler Waters

Range 0 - 500 mg / l Cl.
Using the measuring syringe, take 1 ml of sample. Transfer to the test tube and make-up to the 10 ml mark with deionised water.
For Testing Salt Chlorinator Treated Swimming Pools

Range 0 – 10,000 mg / l NaCl.
Using the measuring syringe, take 0,5 ml of sample. Transfer to a 100 ml volumetric flask then make-up to the 100 ml mark with
deionised water. Cap flask and mix. Fill test tube to the 10 ml mark with solution from the flask.
For Testing Sea Water and Brackish Waters

Range 0 – 50,000 mg / l NaCl.
Using the measurement syringe, take 0,1 ml of sample. Transfer to the 100 ml volumetric flask, then make-up to the 100 ml mark with
deionised water. Cap de flask and mix. Fill test tube to the 10 ml mark with solution from the flask
12
2. - Add one Acidifying CD tablet, crush and mix to dissolve.
3. - Add one Chloride tablet, allow the tablet to disintegrate for one minute then crush any remaining particles and mix. A cloudy
solution indicates the presence of chloride.
4. - Select wavelength 520 nm on photometer.
5. - Take the photometer reading in usual manner(see Photometer instructions). Make blank without tablet.
6. - Consult the Chloride calibration chart (D-101). Select program 7, 54, 55 or 56 (D-105 and D-100).
CONVERSION FACTORS
To convert mg / l of to mg / l of multiply by
Cl NaCl 1,65
NaCl Cl 0,61
Cl CaCO3 1,41
NaCl CaCO3 0,85
Range : 0 - 40 mg / L Cl Chloride 520 nm
ABS 0 2 4 6 8 ABS 0 2 4 6 8
0.0 0 0.4 1.1 21.6 22.0 22.4 22.8 23.2
0.1 0.8 1.2 1.6 1.9 2.4 1.2 23.6 24.0 24.4 24.8 25.2
0.2 2.8 3.2 3.6 4.0 4.4 1.3 25.7 26.1 26.6 27.0 27.5
0.3 4.8 5.2 5.7 6.2 6.6 1.4 27.9 28.4 28.8 29.3 29.7
0.4 7.1 7.5 8.0 8.4 8.9 1.5 30.2 30.6 31.1 31.5 32.0
0.5 9.3 9.8 10.2 10.6 11.0 1.6 32.5 32.9 33.4 33.8 34.3
0.6 11.4 11.8 12.2 12.6 13.0 1.7 34.8 35.2 35.6 36.1 36.6
0.7 13.4 13.9 14.3 14.7 15.1 1.8 37.0 37.5 37.9 38.4 38.8
0.8 15.5 15.9 16.3 16.7 17.1 1.9 39.3 40
0.9 17.5 17.9 18.3 18.7 19.1
1.0 19.5 19.9 20.3 20.7 21.1
Range : 0 - 400 mg / L Cl Chloride 520 nm
ABS 0 2 4 6 8 ABS 0 2 4 6 8
0.0 0 4 1.1 216 220 224 228 232
0.1 8 12 16 19 24 1.2 236 240 244 248 252
0.2 28 32 36 40 44 1.3 257 261 266 270 275
0.3 48 52 57 62 66 1.4 279 284 288 293 297
0.4 71 75 80 84 89 1.5 302 306 311 315 320
0.5 93 98 102 106 110 1.6 325 329 334 338 343
0.6 114 118 122 126 130 1.7 347 352 356 361 366
0.7 134 139 143 147 151 1.8 370 375 379 384 388
0.8 155 159 163 167 171 1.9 393 400
0.9 175 179 183 187 191
1.0 195 199 203 207 211
Rango : 0 - 10000 mg / L NaCl 520nm
ABS 0 2 4 6 8 ABS 0 2 4 6 8
0.0 0 130 1.1 7100 7250 7400 7500 7650
0.1 260 390 520 650 790 1.2 7800 7900 8050 8200 8300
0.2 920 1050 1190 1320 1450 1.3 8500 8600 8750 8900 9050
0.3 1590 1730 1880 2030 2180 1.4 9200 9350 9500 9650 9800
0.4 2330 2480 2630 2780 2930 1.5 9950 10100
0.5 3080 3220 3350 3500 3630
0.6 3770 3900 4050 4170 4300
0.7 4450 4550 4700 4850 4950
0.8 5100 5250 5350 5500 5650
0.9 5800 5900 6050 6200 6300
1.0 6450 6600 6700 6850 7000
Range: 0- 50000 mg/ L NaCl 520nm
ABS 0 2 4 6 8
0.0 0 630
0.1 1290 1960 2630 3300 3950
0.2 4600 5300 5950 6620 7280
0.3 7950 8650 9400 10150 10900
0.4 11600 12400 13100 13900 14600
0.5 15400 16100 16800 17500 18200
0.6 18800 19500 20200 20800 21500
0.7 22200 22900 23500 24200 24900
0.8 25500 26200 26900 27500 28200
0.9 28900 29500 30200 30900 31500
1.0 32200 32900 33600 34200 38200
1.1 35600 36200 36900 37600 38200
1.2 38900 39600 40200 40900 41600
1.3 42400 43100 43800 44600 45300
1.4 46100 46800 47600 48300 49000
1.5 49800 50500
13
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,284 mg/L Cl -
Traceability:The traceability of the DPD Chlorine method settles down with “Chim. anal. , 31, 32(1949)” adapted.
CHLORINE ( DPD ). Code 1.9412.00 (50t) - 1.9474.00 (250t) Photometer Method 520 nm
Test for free, combined and total Chlorine in water 0- 5 mg / l
Chlorine and chlorine-release compounds are widely used for the disinfection of drinking water and swimming pools, for the control of
micro-biological growth in cooling water, and in many other water treatment systems. Accurate measurement of the chlorine residual is
an essential aspect of the control of these chlorination processes.
The chlorine level can be expressed in terms of the free chlorine, combined chlorine or total chlorine. For the majority of applications
measurement of the free chlorine is most important. The DINKO DPD method provides a simple means of measuring free, combined
and total chlorine over the range 0 - 5 mg / l.
METHOD
This DINKO test uses the DPD method developed by Dr. A T Palin and now internationally recognised as the standard method of
testing for Chlorine and other disinfectant residuals. In the DPD method the reagents are provided in tablet form for maximum
convenience and simplicity of use.
Free Chlorine reacts with diethyl-p-phenylene diamine ( DPD ) in buffered solution to produce a pink coloration. The intensity of colour is
proportional to the free Chlorine concentration. Subsequent addition of excess potassium iodide induces a further reaction with any
combined chlorine present. The colour intensity is now proportional to the total Chlorine concentration; the increase in intensity
represents the combined chlorine concentration. So, it is possible to know the free and combined Chlorine concentration in the sample.
The colour intensities are measured using a DINKO Photometer.

DPD nº 1 Tablets / DPD nº 3 Tablets / Round cuvette 16 mm Ø with cap. (4pcs ). Code 1.9365.00 (cuvette used in the chart).
Square cuvette 10 mm with cap. Pk. (100). Code 1.9363.00
Photometer DINKO D-101, use the calibration chart / Photoanalyzer DINKO D-105 or D-100 Photometer select program Nr.6.
PROCEDURE
1. - Rinse test tube sample leaving two or three drops of sample in the tube .
2. - Add one DPD nr. 1 tablet, crush tablet and then fill the test tube with sample to the 10ml mark. Mix to dissolve tablet.
4. - Take Photometer reading immediately. Make zero with sample without tablet.
5. - The result represents the free Chlorine as milligrams per litre test.
6. - If it is desired to measure combined or total Chlorine continue the test on the same test portion.
7. - Add one DPD nr. 3 tablet, crush and mix to dissolve.
8. - Stand for two minutes to total colour development.
9. - Take Photometer reading. Consult Chlorine chart(D-101). Photoanalyzer D-105 and D-100 select program nr. 6.
10. - The result represents the total Chlorine residual as milligrams per litre.
11. - The combined Chlorine is obtained by subtracting the free Chlorine result from the total Chlorine result.
Combined Chlorine = Total Chlorine - Free Chlorine
Range: 0 - 5 mg / L Chlorine 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.03 0.06 0.09 0.13 0.16 0.20 0.24 0.27 0.31
0.1 0.34 0.37 0.41 0.44 0.48 0.51 0.54 0.58 0.61 0.65
0.2 0.69 0.71 0.75 0.78 0.82 0.85 0.88 0.92 0.95 0.99
0.3 1.03 1.07 1.12 1.17 1.21 1.26 1.30 1.35 1.40 1.44
0.4 1.49 1.53 1.58 1.63 1.67 1.72 1.76 1.81 1.86 1.90
0.5 1.95 1.99 2.03 2.08 2.12 2.15 2.19 2.23 2.27 2.31
0.6 2.35 2.39 2.43 2.47 2.51 2.55 2.59 2.63 2.67 2.71
0.7 2.75 2.79 2.82 2.87 2.91 2.95 2.99 3.03 3.08 3.13
0.8 3.18 3.23 3.27 3.32 3.37 3.42 3.47 3.51 3.56 3.61
0.9 3.66 3.71 3.75 3.80 3.85 3.90 3.95 3.99 4.06 4.14
1.0 4.21 4.28 4.35 4.42 4.49 4.57 4.64 4.71 4.78 4.86
1.1 4.93 5.00
Note
A too high Chlorine level (above 10 mg / l ) can cause bleaching of the pink coloration formed in the DPD test and give a false negative
result. If a colourless test solution is obtained when Chlorine is known to be present, check for the possibility of bleaching by repeating
the test on a sample diluted with Chlorine-free water.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,057 mg/L Chlorine
Traceability: The traceability of the method settles down with Standard Method 4500-CIG and USEPA 330.5”
14
CHLORINE. Code 1.9413.00 (50t) - 1.9483.00 (250t) Photometer Method 490 nm
Test for high levels of Chlorine in disinfecting and sterilizing solutions 0- 250 mg / l
Chlorine and chlorine release compounds are widely used for disinfection or sterilizing of water distribution systems and pipe work, plant
and equipment in food processing and pharmaceutical factories. The Chlorine levels used in these applications are higher than those
applied for the simple disinfection of water. Accurate measurement of the Chlorine level is necessary to ensure the intended use.
The DINKO test provides a simple means of measuring the total Chlorine over the range 0 - 250 mg / l.
METHOD
The DINKO test is based on an iodine release method. Chlorine reacts with Potassium iodide in acid solution to release iodine which is
brown in colour. The reagents for test are provided in the form of two tablets for maximum convenience and simplicity of use.
The intensity of the colour produced is proportional to the Chlorine concentration and is measured using a DINKO Photometer.

Acidifying GP Tablet / Chlorine HR Tablet
Round cuvette 16 mm Ø with cap. (4pcs ). Code 1.9365.00 (cuvette used in the chart).
Photometer DINKO D-101, use the calibration chart / Photoanalyzer DINKO D-105 or D-100 Photometer select program nr. 31
PROCEDURE
1. - Fill test tube with sample to the 10 ml mark.
2. - Add one Acidifying GP tablet and one Chlorine HR tablet and Chlorine HR tablet. Crush tablets and mix to dissolve.
3. - Select 490 nm. filter of Photometer. Take Photometer reading. Make zero whit sample without tablet.
4. - Consult Chlorine chart (D-101). Photoanalyzer D-105 and D-100 select program nr. 31
Range: 0 - 250 mg / L Chlorine 490 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 2 4 6 8 10 12 14 16 18
0.1 20 22 24 26 28 30 32 34 36 39
0.2 41 43 45 47 50 52 55 58 60 63
0.3 65 68 70 73 75 78 81 83 86 88
0.4 91 94 97 100 102 105 108 111 114 117
0.5 119 122 125 128 131 134 136 139 142 145
0.6 148 151 153 157 160 163 166 170 173 176
0.7 179 183 186 189 192 196 199 202 205 209
0.8 212 215 218 221 224 228 231 234 237 240
0.9 244 247 250
Note:
For precise determination of lower levels of Chlorine, up to 5 mg/l, Chlorine (DPD) method should be used.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 4,260 mg/L Chlorine
Traceability: The traceability of the method settles down with “Standard Method” SM
CHLORINE DIOXIDE and CHLORITE Code 1.9415.00 (50t) - 1.9451.00 (250t) Photometer Method 520 nm
Test for Chlorine Dioxide and others residuals in water 0- 10,0 mg / l
Chlorine dioxide is used for the disinfection of water in a variety of different applications. Chlorine dioxide is generated by reacting
Chlorine with Sodium chlorite solution. So, water treated with Chlorine dioxide also contain Chlorine and chlorite. Will be necessary to
determine and differentiate between these residuals species.
The DINKO Chlorine dioxide test provide a method of determining Chlorine dioxide, Chlorine free and combined Chlorine, and chlorite,
in treated water.
METHOD
Chlorine dioxide reacts with diethyl-p-phenylenediamine (DPD) in buffered solution to produce a pink colouration. Glycine is used to
prevent the reaction with Chlorine so as to give specific determination of chlorine dioxide.
In the supplementary part of the test the glycine is omitted and it is then possible, by difference, to measure the free chlorine content.
Subsequent addition of potassium iodide induces a further reaction with any combined Chlorine present. Continuation of the test using
an acidification and neutralisation procedure produces a further reaction and in this way the chlorite concentration can be determinate.
The colour intensities at each stage of the test are measured using a DINKO Photometer. It is normal practice to express the
concentration of each component as equivalent Chlorine concentration.
REAGENTS ANDY EQUIPMENT

DPD nr. 1 Tablet / DPD nr. 3 Tablet / DPD Glycine Tablet / DPD Acidifying Tablet / DPD Neutralising Tablet
Round cuvette 16 mm Ø with cap. (4pcs ). Code 1.9365.00 (cuvette used in the chart).
Square cuvette 10 mm with cap. (100pcs). Code 1.9363.00
Photometer DINKO D-101, use the calibration chart / Photoanalyzer DINKO D-105 or D-100 Photometer select program nr. 30
15
PROCEDURE CHLORINE
1. - Rinse a test tube with sample leaving two or three drops. Add one DPD nr. 1 tablet and crush.
2. - Fill a second test tube with sample to 10 ml mark. Add one DPD Glycine tablet, crush and mix.
3. - Add the contents of the second test tube to the first test tube and mix.
4. - Select wavelength 520 nm on photometer. Select program nr.6 whit D-100 and D-105 photometers. Take photometer reading
immediately. Make zero whit the sample without tablets.
5. - Consult the calibration chart (D-101) and note the value (Result G) corresponding to the absorbance reading.
Multiply Result G by 5 to obtain the Chlorine dioxide residual in terms of mg/l Chlorine.
To obtain the Chlorine dioxide residual as mg/l Chlorine Dioxide multiply Result G by 1.9.
Range: 0- 5,0 mg / L 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.03 0.06 0.09 0.13 0.16 0.20 0.24 0.27 0.31
0.1 0.34 0.37 0.41 0.44 0.48 0.51 0.54 0.58 0.61 0.65
0.2 0.69 0.71 0.75 0.78 0.82 0.85 0.88 0.92 0.95 0.99
0.3 1.03 1.07 1.12 1.17 1.21 1.26 1.30 1.35 1.40 1.44
0.4 1.49 1.53 1.58 1.63 1.67 1.72 1.76 1.81 1.86 1.90
0.5 1.95 1.99 2.03 2.08 2.12 2.15 2.19 2.23 2.27 2.31
0.6 2.35 2.39 2.43 2.47 2.51 2.55 2.59 2.63 2.67 2.71
0.7 2.75 2.79 2.82 2.87 2.91 2.95 2.99 3.03 3.08 3.13
0.8 3.18 3.23 3.27 3.32 3.37 3.42 3.47 3.51 3.56 3.61
0.9 3.66 3.71 3.75 3.80 3.85 3.90 3.95 3.99 4.06 4.14
1.0 4.21 4.28 4.35 4.42 4.49 4.57 4.64 4.71 4.78 4.86
1.1 4.93 5.00
PROCEDURE – FREE AND COMBINED CHLORINE, AND CHLORITE

1. - Rinse a test tube with sample leaving two or three drops. Add one DPD nr. 1 tablet, and then fill the test tube with
sample to the 10 ml mark. Mix to dissolve tablet
2. - Take the photometer reading immediately(see photometer instructions). Make zero whit the sample without tablets.
Consult the calibration chart and note the value (Result A).
3. - Continue the test by adding one DPD nr. 3 tablet. Crush, mix to dissolve and stand for two minutes.
4. - Take photometer reading immediately. Use the same blank of step 2. Consult calibration chart and note the
value(Result C) corresponding to the observed absorbance reading.
5. - Continue the test by adding one DPD Acidifying tablet. Crush and mix to dissolve and stand for two minutes.
6. - Add one DPD Neutralising tablet, crush and mix to dissolve.
7. - Take the photometer reading in the usual manner and consult calibration chart. Note the value(Result D) corresponding to the
observed absorbance reading. Use the same zero of 2 and 4 steps.
The results of the tests, in terms of mg/l Chlorine, are calculated from the observed results as follows:
Chlorine Dioxide = 5G
Free Chlorine =A–G
Combined Chlorine =C–A
Chlorite = D – ( C + 4G )
Total Oxidising Capacity =D
Uncertainty:
The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,057mg/L Chlorine
Traceability :
The traceability of the DPD Chlorine method settles down with Standard Methods 4500-CIG and USEPA 330.5”
COLOUR. Code1.9423.00 (50t) Photometer Method 415 - 420 nm

Test for colour in natural and treated waters 10- 500 mg/l Pt (Hazen units)
Pure water exhibits a light blue colour when viewed in depht. This colour may be modified by the presence of organic material, typically
to a yellow or brown colour. An estimate of this colour intensity is used as a simple means of monitoring natural and treated water.
METHOD
The colour of the water is determinate using a DINKO Photometer. The sample should be filtered to remove suspended solids before
analysis to determine the “true colour” due to dissolved matter.
The colour is expressed using the Platinum/Cobalt colour scale(Pt/Co scale). Each unit is equivalent to the colour produced by 1 mg/l
Platinum in the form of Chloroplatinic Acid in the presence of 2 mg/l cobaltous chloride hexahydrate. These units are identical with
“Hazen” or APHA units which have been traditionally used to express results from the visual estimation of water colour.
Syringe Filter
Round cuvette 16 mm Ø with cap. (4 pcs). Code: 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use the calibration chart
16
PROCEDURE
1. - Filter 10 ml of sample using the syringe filter system.
2. - Fill a test tube with filtered sample to the 10 ml mark.
3. - Fill a test tube with deionised water to the 10 ml mark and retain for use as the ZERO tube
4. - Select the filter 420 nm on Photometer D-101. Photometers D-105 and D-100 select program nr. 52
5. - Take photometer reading using the deionised water as the zero. Consult Colour calibration chart (D-101 Photometer)). Select
program nr. 52 (Photoanalyzer D-105 and D-100 Photometer).
Range: 0 - 500 mg / L Pt 420 nm

ABS 0 4 8 ABS 0 4 8
0.00 0 10 20 0.11 286 297 313
0.01 25 35 45 0.12 321 337 353
0.02 50 60 70 0.13 361 376 392
0.03 75 85 95 0.14 400 410 419
0.04 100 110 119 0.15 424 433 443
0.05 124 134 144 0.16 448 454 459
0.06 148 158 168 0.17 462 468 473
0.07 173 182 192 0.19 489 495 500
0.08 197 206 216
0.09 221 231 240
0.10 245 258 274
Samples which contain metallic impurities, dyestuffs or other industrial pollutants may exhibit a different colour to the natural yellow-
brown coloration. This test may not be suitable of samples of this type.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 3,406 mg/L Pt / Hazen, APHA units
Traceability: The traceability of the Colour method settles down with “Standard Methods of Water and Wastewater, APHA - AWWA
- WPOC F , Edición 16 y 17.
COPPER. Code 1.9422 (50t) - 1.9476.00 (250t) Photometer Method 520 nm

Test for free, chelated and total copper in natural and treated water. 0- 5,0 mg / l Cu
Copper occurs naturally in many waters and also result from corrosion of pipes and fittings. The presence of copper in drinking water
can give rise to discoloration or an astringent taste.
Chelate copper compounds are extensively used as algicides in swimming pool water, home aquariums and other waters. Electrolytic
devices which generate copper and silver ions are used in the purification of swimming pool water. The DINKO Copper test provides a
simple means of measuring copper over the range 0 - 5 mg / l. The test is particularly useful since it can be used to measure
specifically the concentrations of free and chelated Copper present in the water.
METHOD
In the method Copper salts are reduced to the cuprous form and then react with a 2,2-Biquinoline-4,4-dicarboxilic salt to form a purple
coloured complex. This provides a measure of the free copper ions present in the sample. In the second stage of the test, a
decomplexing agent is introduced and this induces a further reaction with any chelated copper compounds which might be present.
The reagents are provided in tablet form and the test is simply carried out by adding tablets to a sample of the water. The intensity of
colour produced in the test is proportional to the Copper concentration and is measured using a DINKO Photometer.

Coppercol nº 1 Tablets / Coppercol nr. 2 Tablets
Round cuvette 16 mm Ø with cap. (4pcs ). Code: 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use the calibration chart.
TEST PROCEDURE
1. - Fill test tube sample to the 10 ml mark.
2. - Add one Coppercol nr. 1 tablet, crush and mix to dissolve.
3. - Select wavelength 520 nm on Photometer. Make zero with sample without tablets.
4. - Take Photometer reading in usual manner. Consult the Copper calibration chart(D-101). Select program nr. 12 (D-105 and D-100).
5. - The result represents the free Copper concentration. Stop the test at this stage if only free copper determination is required.
6. - If it is desired to measure chelated or total Copper continue the test on the same test portion.
7. - Add one Coppercol nr. 2 tablet, crush and mix to dissolve.
8. - Take Photometer reading like in the 4 stage.
9. -The result represents the total Copper concentration.
10. -The chelated copper concentration is obtained by subtracting the free Copper concentration from the total copper concentration.
17
Chelated Copper = Total Copper- Free Copper.
Range: 0 - 5,0 mg / L Cu Copper 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 0.06 0.13 0.21 0.28 0.35 0.42 0.49 0.56 0.63
0.1 0.69 0.76 0.83 0.90 0.97 1.04 1.11 1.18 1.26 1.33
0.2 1.40 1.47 1.54 1.61 1.68 1.75 1.82 1.89 1.96 2.04
0.3 2.11 2.18 2.25 2.32 2.39 2.46 2.53 2.60 2.67 2.74
0.4 2.81 2.88 2.95 3.02 3.09 3.16 3.22 3.29 3.36 3.43
0.5 3.50 3.56 3.63 3.70 3.77 3.84 3.90 3.97 4.04 4.12
0.6 4.19 4.26 4.33 4.41 4.48 4.55 4.62 4.70 4.77 4.84
0.7 4.91 5.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,099 mg/L Cu
Traceability: The traceability of the Copper method settles down with USEPA (United States Environmental Protection Agency)
CHEMICAL OXYGEN DEMAND - COD/ 150. Photometer Method 440-450 nm

Code 1.9429.00 10-150 mg/l O2
Evaluation of the contamination degree of residual waters
Chemical Oxygen demand is a vital test for assessing the quality of effluents and waste waters prior to discharge. The Chemical
Oxygen Demand (COD) test predicts the oxygen requirement of the effluent and is used for monitoring and control of discharges, and
for assessing treatment plant performance. The COD test is therefore performed as routine in laboratories of water utilities and industrial
companies and comply with the ISO 15705.
METHOD
In the DINKO COD method, the water sample is oxidised by digesting in a sealed reaction tube with Sulphuric acid and Potassium
dichromate in the presence of a Silver sulphate catalyst. The amount of dichromate reduced is proportional to the chemical Oxygen
demand (COD). A reagent blank is prepared for each batch of tubes so as to compensate for the Oxygen demand of the reagent itself.
Over the range of the test a series of colours from yellow through green to blues are produced. The colours is indicative of the chemical
Oxygen demand and is measured using a DINKO Photometer. The results are expressed as milligrams of Oxygen consumed per litre of
sample.

Kit COD 25 tubes. Range 10 -150 ppm. Code 1.9429.00
Heater block D-65. Code 1.8082.00
Block 24 tubes of 16 mm. Ø. Code 1.8085.00 and Safety-bell. Code: 1.8089.00 for D-65 Heater block
Heater block D-64D, with block of 12 tubes. Code 1.8081.10
Photoanalyzer D-105. Code 1.9336.00 and Photometer D-100. Code 1.9301.00. Select program nr. 9
Photometer D-101. Code 1.9333.00. Use the calibration chart
WORKING PRACTICE
COD test reagents are light sensitive. Store tubes in the original container and keep the box closed when not in use. Inspect tubes
before use, do not use any show green discoloration. The DINKO COD test should be carried out in accordance with good laboratory
working practice. The reagent tubes contain 84% Sulphuric acid and must be handled with care. The use of appropriate protective
clothing, gloves and safety spectacles is recommended. In the event of skin or eye contact, or spillage, wash immediately with large
amounts of water. Special care should be taken when opening the reagent tubes to add the water sample as heat will be produced and
gases may be evolved. Samples containing cyanide or sulphide will release toxic fumes and for such samples the test must always be
carried out in a fume cupboard. It is recommended that the test be conducted using safety-bell. Reagent tubes should not be opened
whilst hot as pressure build-up may cause acid spillage.
BLANK REAGENT
In this test a reagent ZERO is used instead of Water Zero referred to in the general photometer operating instructions. The reagent zero
is prepared by adding deionised or distilled water to the reagent tube (see Test Procedure, Step 4 ) and then digesting the tube in the
same manner as for the sample.
It is not necessary to prepare a reagent zero each time the test is carried out. The reagent zero tube may be prepared weekly and use
repeatedly with all samples prepared from the same batch of reagent tubes. The reagent zero should be stored in the dark.
SAMPLE PREPARATION
Some water samples may contain undissolved or particulate material. Such samples may be homogenised in prior to the test in order to
improve accuracy and reproducibility.
TEST PROCEDURE
1.- Turn on Heater block D-65, set the control to the 150º C. Allow the heater up to temperature.
2.- Remove the cap of the COD tube and add 2 ml of sample using a measuring syringe or a standard laboratory pipette.
3.- Replace the cap tightly and invert tube gently to mix contents. The tube will become hot on mixing. Label the tube using the labels
provided and place the tube in the Heater block. Place the safety-bell in position.
4.- Prepare a Reagent Zero by repeating steps 2 and 3 using 2 ml of deionised or distilled water in place of the sample.
5.- Put the COD tubes in the Heater for two hours then turn off the Heater.
6.- Carefully remove each tube, and then transfer to a test tube rack.
7.- Allow the tube to cool to room temperature.
8.- Select the filter 440 nm on Photometer D-101. On Photoanalyzer D-105 and D-100 select program nr. 9
18
9.- Insert the Reagent Zero tube in the Photometer D-101 and adjust to read 1.000 Abs. Remove the Reagent Zero tube and insert
the sample COD tubes. Take the photometer reading.
For the Photoanalyzer D-105 and D-100 the usual photometer operating sequence is reversed. Take the reading as follows:
Firstly insert the Sample Tube and use this to set the instrument ( Zero Adjust).
Then insert the Reagent Zero and use this to take the photometer reading.
10.- For the Photometer D-101 use the calibration table of instruction manual. For the Photoanlayzer D-105 and Photometer D-100
select programme nr. 9.
INTERFERENCES
Every COD tubes have mercuric sulphate that suppress interference of chlorides from up 2,500 mg / l. in the sample. Samples
containing above this level should be diluted.
RANGE: 10 - 150 mg / L O2 PPM Oxygen 440 nm
ABS. 0 1 2 3 4 5 6 7 8 9
0,5 150 148 145 142 139
0,6 135 132 128 125 122 118 115 111 108 105
0,7 101 98 95 91 88 84 81 78 74 71
0,8 68 64 61 57 54 51 47 44 41 37
0,9 34 30 27 24 20 17 14 10 7 3
1,0 0
For the instrument periodical verification or realization of calibration charts for other photometers can be used to the following COD
standards:
Packing of 60 ml: 50 ppm. Cod. 9971 / 100 ppm. Cod. 9972 / 500 ppm. Cod. 9974 / 1500 ppm Cod. 9977 / 5000 ppm. Cod. 9978 /
15000 ppm. Cod. 9980
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 5,673 mg/L O2
Traceability: The traceability of COD method settles down with “ Standard Methods of Water and Wastewater, APHA - AWWA –
WPOC F”, 19th Edition, which has been classified by the “ American Library of Congress ” like IBSN 0 - 87553 –
207-1. Also traceability with ISO 15705, EPA 410-4 and 5220D.
CHEMICAL OXYGEN DEMAND - COD/ 2000. Code 1.9430.00 Photometer Method 577- 580 nm
Evaluation of the contamination degree of residual waters 100 - 2000 mg/l O2
Chemical oxygen demand is a vital test for assessing the quality of effluents and waste waters prior to discharge. The Chemical Oxygen
Demand (COD) test predicts the oxygen requirement of the effluent and is used for monitoring and control of discharges, and for
assessing treatment plant performance. The COD test is therefore performed as routine in laboratories of water utilities and industrial
METHOD
In the Dinko COD method, the water sample is oxidised by digesting in a sealed reaction tube with sulphuric acid and potassium
dichromate in the presence of a Silver sulphate catalyst. The amount of dichromate reduced is proportional to the chemical oxygen
demand (COD). A reagent blank is prepared for each batch of tubes so as to compensate for the oxygen demand of the reagent itself.
oxygen demand and is measured using a DINKO Photometer. The results are expressed as milligrams of Oxygen consumed per litre of
sample.

Kit COD 25 tubes. Range 100 - 2000 ppm. Code 1.9430.00
Heater block D-65. Code:1. 8082.00. Block 24 tubes of 16 mm. Ø . Code 1.8085.00. Safety-bell. Code 1.8089.00
Heater Block, with block of 12 tubes. Code 1.8081.10
Photoanalyzer D-105. Code 1.9336.00 / Photometer D-101. Code 1.9333.00/ Photometer D-100. Code 1.9301.00
WORKING PRACTICE
working practice. The reagent tubes contain 84% sulphuric acid and must be handled with care. The use of appropriate protective
BLANK REAGENT
In this test a reagent blank is used instead of Water Zero referred to in the general photometer operating instructions. The reagent
blank is prepared by adding deionised or distilled water to the reagent tube ( see Test Procedure, Step 4 ) and then digesting the tube in
the same manner as for the water sample.
repeatedly with all samples prepared from the same batch of reagent tubes. The reagent zero should be stored in the dark.
19
SAMPLE PREPARATION
Some water samples may contain undissolved or particulate material. Such samples may be homogenised prior to the test in order to
improve accuracy and reproducibility.
TEST PROCEDURE
2.- Remove the cap of the COD tube and add 2 ml of sample using a measuring syringe or a standard laboratory pipette.
4.- Prepare a Reagent Zero by repeating steps 2 and 3 using 2 ml of deionised or distilled water in place of the sample.
6.- Carefully remove each tube and then transfer to a test tube rack.
8.- Select the filter 580 nm on the Photometer D-101. On Photoanalyzer D-105 and D-100 select program nr.10
9.- Take the photometer reading in the usual manner (see photometer instructions)
10.- Consult chart (Photometer D-101). Select programme nr. 10 in the Photoanalyzer D-105 and D-100
INTERFERENCES
Every COD tubes have mercuric sulphate that suppress interference of chlorides from up 2,500mg / l. in the sample. Samples
containing above this level should be diluted.
RANGE: 100 - 2000 mg / L O2 PPM Oxygen 577 - 580nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 28 55 83 110 140 170 195 225 255
0.1 285 315 345 375 405 435 465 495 525 555
0.2 580 610 640 670 700 730 760 790 820 850
0.3 880 910 940 970 1000 1030 1060 1090 1125 1155
0.4 1185 1215 1245 1280 1310 1340 1370 1400 1430 1465
0.5 1495 1530 1565 1595 1630 1665 1700 1735 1770 1805
0.6 1840 1875 1910 1945 1980 2000
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 42,58mg/L O2
Traceability:
The traceability of COD method settles down with “ Standard Methods of Water and Wastewater, APHA - AWWA - WPOC F”, 19th
Edition, which has been classified by the “ American Library of Congress ” like IBSN 0 - 87553 - 207 – 1. Also traceability with
ISO 15705, EPA 410-4 and 5220D.
standards:
Packing of 60 ml: 50 ppm. Cod. 9971 / 100 ppm. Cod. 9972 / 500 ppm. Cod. 9974 / 1500 ppm. Cod. 9977 / 5000 ppm. Cod. 9978 /
15000 ppm. Cod. 9980
CHEMICAL OXYGEN DEMAND - COD / 20000. Code 1.9431.00 Photometer Method 577- 580 nm
Evaluation of the contamination degree of residual waters 1000-20000mg/l O2
Chemical Oxygen demand is a vital test for assessing the quality of effluents and waste waters prior to discharge. The Chemical
Oxygen Demand (COD) test predicts the oxygen requirement of the effluent and is used for monitoring and control of discharges, and
for assessing treatment plant performance. The COD test is therefore performed as routine in laboratories of water utilities and industrial
METHOD
In the Dinko COD method, the water sample is oxidised by digesting in a sealed reaction tube with sulphuric acid and potassium
dichromate in the presence of a Silver sulphate catalyst. The amount of dichromate reduced is proportional to the chemical Oxygen
demand (COD). A reagent blank is prepared for each batch of tubes so as to compensate for the oxygen demand of the reagent itself.
oxygen demand and is measured using a DINKO Photometer. The results are expressed as milligrams of Oxygen consumed per litre of
sample.
Kit COD 25 tubes. Range 1000 - 20000 ppm. Code 1.9431.00
Heater block D-65. Code 1.8082.00 / Block 24 tubes of 16 mm. Ø. Code 1.8085.00 / Safety-bell for D-65. Code 1.8089.00
Heater block with block of 12 tubes D-64D. Code 1.8081.10
Photoanalyzer D-105. Code 1.9336.00 / Photometer D-101. Code 1.9333.00 / Photometer D-100. Code 1.9301.00
WORKING PRACTICE
working practice. The reagent tubes contain 60% sulphuric acid and must be handled with care. The use of appropriate protective
20
BLANK REAGENT
In this test a reagent ZERO is used instead of Water Zero referred to in the general photometer operating instructions. The reagent
blank is prepared by adding deionised or distilled water to the reagent tube ( see Test Procedure, Step 4 ) and then digesting the tube in
the same manner as for the water sample.
repeatedly with all samples prepared from the same batch of reagent tubes. The reagent cero should be stored in the dark.
SAMPLE PREPARATION
Some water samples may contain undissolved or particulate material. Such samples may be homogenised in a blender prior to the test
in order to improve accuracy and reproducibility.
TEST PROCEDURE
2.- Remove the cap of the COD tube and add 0,2 ml of sample using a measuring syringe or a laboratory pipette.
4.- Prepare a Reagent Zero by repeating steps 2 and 3 using 2ml of deionised or distilled water in place of the sample.
6.- Carefully remove each tube and then transfer to a test tube rack.
8.- Select the filter 580 nm on Photometer D-101. On Photoanalyzer D-105 and D-100 select program nr.11
9.- Take the photometer reading in the usual manner ( see photometer instructions)
10.- Consult calibration chart (Photometer D-101). Select programme nr. 11 for D-105 and D-100.
INTERFERENCES
Every COD tubes mercuric sulphate that suppress interference of chlorides from up 25,000mg / l. in the sample. Samples containing
above this level should be diluted.
RANGE: 1000 - 20000 mg / L O2 PPM Oxygen 577- 580 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 280 550 830 1100 1400 1700 1950 2250 2550
0.1 2850 3150 3450 3750 4050 4350 4650 4950 5250 5550
0.2 5800 6100 6400 6700 7000 7300 7600 7900 8200 8500
0.3 8800 9100 9400 9700 10000 10300 10600 10900 11250 11550
0.4 11850 12150 12450 12800 13100 13400 13700 14000 14300 14650
0.5 14950 15300 15650 15950 16300 16650 17000 17350 17700 18050
0.6 18400 18750 19100 19450 19800 20000
Traceability:
The traceability of COD method settles down with “ Standard Methods of Water and Wastewater, APHA - AWWA - WPOC F”, 19th
Edition, which has been classified by the “ American Library of Congress ” like IBSN 0 - 87553 - 207 – 1. Also traceability with
ISO 15705, EPA 410-4 and 5220D.
standards:
Packing of 60 ml: 50 ppm. Cod. 9971 / 100 ppm. Cod. 9972 / 500 ppm. Cod. 9974 / 1500 ppm. Cod. 9977 / 5000 ppm. Cod. 9978 /
15000 ppm. Cod. 9980
CYANURIC ACID. Code 1.9410.00 (50t) - 1.9465.00 (250t) Photometer Method 520 nm
Test for Cyanuric Acid in swimming pool water 0 - 200 mg / l
Cyanuric Acid is used as Chlorine stabiliser in swimming pool water. The recommended Cyanuric Acid level is about 30 to 200 mg/l or
less in some countries. The use of chloroisocyanurate based chlorine donors increase the Cyanuric Acid level.
The DINKO test is a simple method of measuring Cyanuric Acid.
METHOD
The test is based on a single tablet reagent containing Melamine and buffer. The Cyanuric Acid reacts with Melamine in buffered
solution to produce an insoluble complex. This is observed as a turbidity in the test sample. The degree of turbidity is measured using a
DINKO Photometer.

Cyanuric Acid Tablets / Round cuvette 16 mm Ø (4pcs). Code 1.9365.00
Photometer DINKO model D-101, use calibration chart. DINKO model D-105 or D-100 select program nr.13.
21
PROCEDURE
2. - Add one Cyanuric Acid tablet , crush and mix to dissolve. A cloudy solution indicate the presence of Cyanuric Acid.
3. - Stand for two minutes then mix again
4. - Select the filter 520 nm on Photometer.
5. - Take the Photometer reading. Make the zero with the sample without tablet.
6. - Consult the Cyanuric Acid calibration chart. Select the program 13 for photometers D-105 or D-100.
The range of the test is 0 - 200 mg / l. However when a test result of 100 mg/L or over is obtained, the following dilution technique is
recommended in order to obtain a more accuracy.
1. - Fill the tube sample to 10ml mark with sample.

2. - Pass the 10 ml from the tube sample to an flask of 100 ml and to complete to 100ml mark with distilled water. Mix.
3. - Fill a sample tube to 10ml mark with the above solution.
4. - Repeat test with this sample. The obtained result should multiply for 10 to obtain the concentration of Cyanuric Acid.
Range: 0 - 200 mg / L Cyanuric Acid 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 1.1 2.0 3.0 3.9 4.9 5.9 6.8 7.8
0.1 8.8 9.7 10.7 116 12.6 13.6 14.6 15.5 16.4 17.4
0.2 18.4 19.3 20.3 21 22 23 24 25 26 27
0.3 28 29 30 31 32 33 34 35 36 37
0.4 38 39 40 41 42 44 45 46 48 49
0.5 51 52 53 55 56 58 59 60 62 63
0.6 65 66 68 69 70 72 73 75 76 77
0.7 79 80 82 83 84 86 87 88 90 91
0.8 92 94 95 96 98 99 100 102 103 104
0.9 106 107 108 110 111 112 114 115 116 118
1.0 119 120 122 125 127 129 131 133 135 137
1.1 139 141 143 145 147 149 152 154 156 158
1.2 160 162 164 166 168 170 171 173 175 177
1.3 179 181 183 185 187 189 190 192 194 196
1.4 198 200
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 2,835 mg/L Cyanuric Acid.
DEHA (N,N-Diethylhydroxylamine). Code 1.9439(50t)-1.9505.00(250t) Photometric Method 550 nm.

Test for boiler and cooler water systems in natural and treated water 0.01- 0,500 mg / l DEHA
METHOD
The Dinko test is based on N,N-Diethylhydroxylamine (DEHA) or other oxygen scavengers present in the sample reacting with Ferric
Iron to produce Ferrous Iron. The Ferrous Iron concentration then forms a purple colour proportional to the concentration of the oxygen
scavenger. Ferrous Iron already present in the sample will interfere, hence the method is defined as DEHA + Fe(II). To correct for any
Ferrous Iron not introduced during the method use the Correction Fe II Procedure. This will remove any offset and present the ‘true’
DEHA result as mg/l DEHA.

DEHA Tablets / DEHA Solution / DEHA Iron Correction Solution / Two 1 ml Syringes
Photometer D-100, D-101 and D-105 / Round Test Tubes, 10 ml glass code 1.9365.00
TEST PROCEDURE DEHA + Fe(II)

1 - Fill test tube with sample to the 10 ml mark.
2 - Add one DEHA tablet, crush and mix until completely dissolved.
3 - Add 0,5 ml of DEHA test solution with the first syringe, mix the solution and cap the photometer tube.
4- The solution is photosensitive. In order to prevent inaccurate results, place the tube in the photometer with the light cap in place.
5- Stand for 10 minutes to allow full colour development.

6- Select 550 nm filter on the photometer and take the reading in the usual manner. The result is displayed in mg/l DEHA for the
programmed test on Photometer D-100 and D-105 or absorbance to read on ppm/absorbance table calibration below.
The test may be terminated at this stage if the sample is known to contain no Ferrous Iron.
TEST PROCEDURE – Fe(II) Correction

1- Fill test tube with the same sample to the 10 ml mark.
2- Add one DEHA tablet, crush and mix until completely dissolved.
3- Add 0,5 ml of DEHA Iron Correction solution with the second syringe, mix the solution and cap the photometer tube.
4- The solution is photosensitive. In order to prevent inaccurate results, place the tube in the photometer with the light cap in place.
5- Stand for 10 minutes to allow full colour development.
6- Select 550 nm filter and take the reading in the usual manner. Deduce the mg/l DEHA from the first result.
22
INTERFERENCES
This method reacts with similar oxygen scavengers (carbohydrazide, DEHA, hydroquinone, iso-ascorbic acid [ISA], methylethyl
ketoxime [MEKO]) and does not differentiate samples containing more than one type of oxygen scavenger.
During colour development the samples must be protected from light.
The test has been calibrated at 18°C, sample temperature during the test should be maintained as close to this temperature as possible.
Samples of a temperature lower than 18°C will give a low response; samples of a higher temperature will give a high response.
For a standard in the region of 0,2 mg/l the result will drift by approximately 0.017 mg/l for every 5°C away from 18°C (ie at 23°C the
instrument would give a result of 0,217 mg/l).
For a result in the region of 0,4 mg/l the result will drift by approximately 0.03 mg/l for every 5°C away 18°C (ie at 23°C the instrument
would give a result of 0,43 mg/l).
Any chemical that will reduce ferric iron, or that will complex with iron strongly, will interfere. The species below will interfere at the levels
indicated:
Borate >500 mg/l, Cobalt >0,025 mg/l, Copper >8 mg/l, Hardness >1000 mg/l, Manganese >0,8 mg/l, Molybdenum >80 mg/l, Nickel
>0,8 mg/l, Phosphate >10 mg/l, Phosphonates >10 mg/l, Sulphate >1000 mg/l, Zinc >50 mg/l.
Notes
1- If carrying out the correction procedure for ferrous iron and on addition of the DEHA tablet no colour is produced, this indicates there
is no ferrous iron in the sample and the correction procedure isn’t necessary.
2- To convert a mg/l reading into ppb, multiply the result by 1000.
Range: 0,01- 0,500 mg/ L DEHA 550 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.005 0.012 0.019 0.026 0.032 0.039 0.046
0.1 0.053 0.059 0.066 0.073 0.080 0.087 0.093 0.100 0.107 0.114
0.2 0.120 0.127 0.134 0.141 0.147 0.154 0.162 0.169 0.176 0.183
0.3 0.190 0.197 0.204 0.212 0.219 0.226 0.234 0.241 0.248 0.256
0.4 0.263 0.270 0.277 0.285 0.292 0.299 0.307 0.314 0.321 0.329
0.5 0.336 0.343 0.351 0.358 0.365 0.372 0.380 0.386 0.393 0.400
0.6 0.407 0.414 0.420 0.427 0.434 0.441 0.448 0.454 0.461 0.468
0.7 0.475 0.482 0.488 0.495
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,099 mg/L DEHA
Traceability: The traceability of the method settles down with Ishii and Koh, Bunseki Kagaku, 28 473 (1979)
FLUORIDE. Code 1.9433.00 (50t) - 1.9481.00 (200t) Photometer Method 580 - 577nm
Test for fluoride in natural and treated water. 0-1,5 mg / l
Fluoride occurs naturally in some ground waters and is often introduced into drinking water for the prevention of tooth decay. Excessive
amounts of fluoride are however objectionable and can cause tooth discolouration. The DINKO Fluoride test provides a simple method
to monitoring fluorides in natural waters, and for the control of fluoridation plant at water works.
METHOD
Zirconyl Chloride and Eriochrome Cyanine R are reacted in acid solution to form a red coloured complex. This colour is destroyed by
fluoride ions to give the pale yellow colour of the Eriochrome Cyanine. Differing amounts of fluoride thus produce a range of colours
from red to yellow.
This method is substantially free from interferences wich normally beset chemical methods of fluoride testing. The interference from
aluminium, iron and complexes is eliminated by making the solution alkaline in the first stage of the test procedure. Interferences from
calcium, phosphates and sulphates should not be significant at the levels normally encountered in natural and drinking waters.
In the Fluoride test two tablet reagents are used. The test is simply carried out by adding one of each tablet to a sample of the water.
The colour produced in the test is indicative of fluoride concentration and is measured using a DINKO Photometer.

Fluoride Nº 1 Tablet / Fluoride nr. 2 Tablet
Round cuvette 16 mm Ø with cap. (4pcs ). Code: 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use the calibration chart / DINKO D-105 Photoanalyzer and D-100 Photometer select program nr. 33.
PROCEDURE
1. - Fill test tube with sample to 10 ml mark.
2. - Add one Fluoride nr. 1 tablet, crush and mix to dissolve.
3. - Add one Fluoride nr. 2 tablet, crush and mix to dissolve.
4. - Stand for 5 minutes to allow full colour development.
5. - Take Photometer reading(see Photometer Instructions). Make zero with sample without tablets.
6. - Consult Fluoride chart (D-101, 580 nm). Select program nr. 33 (D-105 and D-100)
23
Range: 0 -1,5 mg / l Fluoride 580 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.2 1.50 1.48 1.43 1.39 1.35 1.30 1.26
0.3 1.21 1.16 1.10 1.03 0.97 0.91 0.85 0.79 0.73 0.70
0.4 0.66 0.62 0.58 0.54 0.51 0.47 0.44 0.41 0.38 0.35
0.5 0.33 0.30 0.26 0.22 0.18 0.14 0.10 0.06 0.02 0.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,057mg/L F
Traceability: The traceability of Zirconyl Chloride method settles down with SM
HARDNESS, TOTAL. Code1.9434.00 (50t) - 1.9479.00 (250t) Photometric Method 577-580 nm

Test hardness in natural and treated water 0 - 500 mg / l CaCO3
Water hardness is caused by the presence of calcium and magnesium salts. High levels of hardness prevent the formation of lather with
soap, and can cause scaling in water systems, particularly boilers, heat exchangers and steam generating plant.
The Hardness test provides a simple method of checking water hardness over the range 0 - 500 mg / l CaCO3
METHOD
The Hardness test is based on a unique colorimetric method. The reagents are provided in tablet form and the test is carried out simply
by adding the appropriate tablets to a sample of the water.
Under the controlled conditions of the test calcium and magnesium ions react with Hardicol indicator to produce a purple coloration. The
intensity of the colour is proportional to the total hardness of the water and is measured using a DINKO Photometer.
Hardicol nº 1 Tablet / Hardicol nº 2 Tablet

Round cuvette 16 mm Ø with cap.(4pcs ). Code 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use calibration chart. Filter 580 nm.
PROCEDURE
2. - Fill test tube with sample to the 10ml mark..
3. - Add one Hardicol nr. 1 tablet, crush and mix to dissolve..
4. - Add one Hardicol nr. 2 tablet, crush and mix to dissolve. Ensure all particles are completely dissolved.
6. - Select the filter 580 nm on the Photometer D-101. On the Photoanalyzer D-105 and D-100 select program nr. 25.
7. -Take Photometer reading in the usual manner. Make zero with sample without tablets.
8. - Consult Hardness calibration chart(D-101). Select program nr. 25 (D-105 and D-100).
INTERFERENCES
1. - Unusually high levels of iron, above 10 mg/l, will cause low results for total hardness.
2. - The pH required in the test is closely controlled by buffer mixture included in the tablet formulation.
However, to avoid exceeding the buffer capacity strongly acid or alkaline samples should be adjusted to within the pH range 4 to
10, prior to the start of the test.
Notes
1 - The expression of hardness results sometimes causes confusion. It is normal practice to express the results of hardness test as
mg /l de CaCO3 (calcium carbonate). This is merely a convention to allow the comparison of different results and does not
necessarily indicate that the hardness is present in the water in this form.
Results may also be expressed as mg/l Ca. To convert CaCO3 mg/l to mg/l Ca multiply by 0,4
2 - Some relations of interest are the following: 1º dH = 1.25º e H = 1.8 f H = 17.8 mg / CaCO3
where dH = German hardness ; eH = English hardness ; f H = French hardness.
Water below 90 mg in Calcium carbonate have a light hardness, between 90 and 180 normal hardness, between 180 and
270 mg moderate hardness, and between 270 and 500 mg high hardness.
3 - This test measure the total hardness. The specific hardness of Calcium or Magnesium can be determinate using the Calcium and
Magnesium kits.
24
Total Hardness CaCO3 580 nm
ABS 0 5 ABS 0 5
0.08 0 4 0.23 162 169
0.09 9 14 0.24 176 183
0.10 19 24 0.25 190 197
0.11 28 33 0.26 206 216
0.12 38 42 0.27 227 237
0.13 47 52 0.28 247 257
0.14 57 61 0.29 267 278
0.15 66 71 0.30 288 298
0.16 75 80 0.31 314 332
0.17 85 90 0.32 350 368
0.18 94 99 0.33 386 404
0.19 106 113 0.34 425 446
0.20 120 127 0.35 467 485
0.21 134 141 0.36 500
0.22 148 155
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 14,18mg/L CaCO3
Traceability:The traceability of Hardicol method settles down with Dr. A T Palin.
HYDRAZINE. Code 1.9435.00 (50t) Photometer Method 450- 420 nm.

Test for Hydrazine in industrial water 0- 0,5 mg / l N2 H4
Hydrazine is used as an oxygen scavenger in high pressure boilers and steam raising plant. Hydrazine is particularly advantageous in
that it does contribute solids to the boiler water. The Hydrazine test provides a simple means of measuring levels in boiler feed water
and boiler water over the range 0 - 0,5 mg/l.
METHOD
The Hydrazine test uses a special reagent powder containing p-Dimethylaminebenzaldehyde in acidic formulation that react with
Hydrazine to produce a yellow coloration. The intensity of colour produced is proportional to the hydrazine concentration and is
measured using a DINKO Photometer.

Hydrazine Test Powder / Scoop, 1g approx. / Round cuvette 16mm Ø with cap. (4pcs ). Code 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use the calibration chart / DINKO D-105 Photoanalyzer and D-100 Photometer select program nr.34.
PROCEDURE
1. - Filter sample if necessary to obtain clear solution.
2. - Take two test tubes A and B.
3. - To each tube add one level scoop(1g) of Hydrazine Test Powder.
4. - Fill test tube A with sample to 10 ml mark. Tap and mix to dissolve.
5. - Fill test tube B with deionised water to 10 ml mark. Tap and mix to dissolve.
7. - Select the filter 420 nm on photometer D-101.On Photoanalyzer D-105 and D-100 select program nr. 34
8. - Take Photometer reading(see Photometer instructions). Use Tube B as the zero to set the instrument.
9. - Consult the Hydrazine calibration chart (D-101). Select program nr. 34(D-105 and D-100)
Range: 0 - 0, 5 mg / L N 2 H4 Hydrazine 420 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.00 0.01 0.01 0.02 0.02 0.03 0.03 0.04 0.04
0.1 0.05 0.05 0.05 0.06 0.07 0.07 0.08 0.08 0.09 0.09
0.2 0.10 0.10 0.11 0.11 0.12 0.12 0.13 0.13 0.14 0.14
0.3 0.15 0.16 0.16 0.17 0.17 0.18 0.18 0.19 0.19 0.20
0.4 0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.24 0.24 0.25
0.5 0.25 0.26 0.26 0.27 0.27 0.28 0.28 0.29 0.29 0.30
0.6 0.30 0.31 0.31 0.32 0.32 0.33 0.33 0.34 0.34 0.35
0.7 0.35 0.35 0.36 0.36 0.37 0.37 0.38 0.38 0.39 0.39
0.8 0.40 0.40 0.41 0.41 0.42 0.42 0.43 0.43 0.44 0.44
0.9 0.45 0.46 0.46 0.47 0.47 0.48 0.48 0.49 0.49 0.50
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,071 mg/L N2 H4
Traceability: The traceability of Hydrazine method settles down with ASTM D-1385-78,376(1979)
25
HYDROGEN PEROXIDE. Code 1.9436.00 (50t) - 1.9485.00 (250t) Photometer Method 520 nm
Test for Hydrogen Peroxide in water 0 -2,0mg / l
Hydrogen Peroxide is used in various water treatment process and it is important the control of concentration.
The Dinko test provides a simple means of measuring Hydrogen Peroxide levels over the range 0- 2.0 mg/l.
METHOD
Hydrogen Peroxide react with Potassium Iodide under slightly acid conditions, and in the presence of a catalyst, to release iodine into
solution. The iodine then reacts with diethyl-p-pheneylendiamine (DPD) to produce a pink coloration. In the Dinko test the reagents are
combined in the form of a single tablet and the test is simply carried out by adding a tablet to a sample of water.
The intensity of colour produced is proportional to the Hydrogen Peroxide concentration and is measured using a DINKO Photometer.

Hydrogen Peroxide LR Tablet / Round cuvette 16mm Ø with cap. (4pcs ). Code: 1.9365.00. (cuvette used in the chart)
PROCEDURE
1. - Rinse test tube with sample leaving 2 to 3 drops of sample in the tube.
2. - Add one Hydrogen Peroxide LR Tablet, crush and then fill the tube with sample to 10 ml mark. Mix to dissolve tablet.
3. - Stand two minutes to allow full colour development. Select filter of 520 nm on the Photometer.
4. - Take Photometer reading. Make zero using sample without tablets.
5. - Consult chart, Photometer D-101. Select program nr.35, Photoanalyzer D-105 and D-100
Notes
1- The sample should be free of other oxidizing agents such as Chlorine, Bromine etc. as these react in similar manner and will
interference with the test. It is unlikely that these oxidizing agents will be used in conjunction with Hydrogen Peroxide and, under
normal circumstances, will not usually coexist in solution.
2- For measuring high levels of Hydrogen Peroxide used in industrial processes, use the Hydrogen Peroxide Test 0-100 mg/l.
Range: 0 -2,0 mg / l Hydrogen Peroxide 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.01 0.03 0.04 0.06 0.08 0.09 0.11 0.12
0.1 0.14 0.15 0.17 0.19 0.20 0.22 0.23 0.25 0.26 0.28
0.2 0.30 0.31 0.33 0.34 0.36 0.37 0.39 0.41 0.43 0.45
0.3 0.47 0.49 0.51 0.53 0.55 0.57 0.59 0.61 0.63 0.65
0.4 0.67 0.69 0.71 0.73 0.75 0.77 0.79 0.81 0.83 0.85
0.5 0.87 0.89 0.91 0.93 0.95 0.97 0.99 1.01 1.04 1.06
0.6 1.09 1.11 1.14 1.17 1.19 1.22 1.24 1.27 1.29 1.32
0.7 1.35 1.37 1.40 1.44 1.48 1.52 1.55 1.59 1.63 1,67
0.8 1.71 1.75 1.79 1.83 1.87 1.91 1.95 2.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,043 mg/L H2 O2
Traceability: The traceability of Hydrogen Peroxide method settles down with SM
HYDROGEN PEROXIDE. Code 1.9437.00 (50t) - 1.9452.00 (250t) Photometer Method 490 nm
Test for high level of Hydrogen Peroxide in water. 0- 100 mg / l
Hydrogen Peroxide is used as bleach and oxidizing agent in a number of industrial processes like textile bleaching, commercial
laundering and paper manufacturing. It is important in such processes to control the Hydrogen Peroxide concentration so as to achieve
the bleaching effect without causing damage.
The DINKO test provides a simple means to measure the Hydrogen Peroxide concentration in the water over the range 0-100 mg / l.
MÉTHOD
Hydrogen Peroxide reacts with Potassium Iodide under acid conditions to release Iodine which gives a yellow solution. A catalyst is
used to speed up the rate of reaction. The reagents are provided in the form of two tablets. The test is simply carried out by adding one
of each tablet to a sample of the water.
The intensity of the colour produced in the test is proportional to the Hydrogen Peroxide concentration and is measured using Dinko
Photometer

Hydrogen Peroxide HR Tablet / Acidifying PT Tablet
Round cuvette 16mm. Ø with cap (4pcs). Code 1.9365.00. (cuvette used in the chart)/ Square cuvette 10 mm with cap. (100pcs). Code
1.9363.00
PROCEDURE
2. - Add one Acidifying PT tablet and one Hydrogen Peroxide HR tablet. Crush and mix to dissolve.
3. - Select 490 nm filter.
4. -Take the Photometer reading. Make zero with sample without tablets.
5. - Consult the chart, Photometer D-101. Select program nº 36, Photoanalyzer D-105 and D-100
26
Range: 0 - 100 mg / l Hydrogen Peroxide 490 nm
ABS 0 1 2 3 4 5 6 7 8 9
0,0 0,4 1,5 2,6 3,7 4,8 5,9 7,0 8,1
0,1 9,2 10,2 11,4 12,5 13,6 14,6 15,7 16,8 17,9 19,0
0,2 20 21 23 24 25 27 28 29 31 32
0,3 33 35 36 37 38 40 41 43 44 46
0,4 47 49 50 51 53 54 56 57 59 60
0,5 62 63 65 66 68 69 71 72 74 75
0,6 77 78 80 82 83 85 86 88 89 91
0,7 92 94 96 97 99
1. – The sample should be free of other oxidizing agents such as Chlorine Bromine as these react in similar manner and will
interfere with the test. It is unlikely that these oxidizing agents will be used in conjunction with Hydrogen Peroxide and, under
normal circumstances, will not usually coexist in solution.
2. - To measure low levels of Hydrogen Peroxide, use the Hydrogen Peroxide Low Range test.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 2,129 mg/L H2 O2
Traceability: The traceability of Hydrogen Peroxide method settles down with SM
IRON. Code 1.9443.00 (50t) - 1.9500.00 (250t) Photometer Method 520 nm

Test for Iron in natural, treated and industrial water 0 – 5,0 mg / l
Iron occurs widely in nature and is found in many natural and treated waters. Iron is an objectionable constituent in both domestic and
industrial water supplies. The presence of Iron affects the taste of beverages and causes unsightly staining of laundered clothes,
plumbing fittings, swimming pool surfaces and the like. The formation of insoluble iron deposits is troublesome in many industrial
applications and in the agricultural water uses such as drip feed irrigation. In industry iron salts occur trough corrosion of plant and
equipment, and from industrial process.
The Dinko Iron test provides a simple method for the determination of both ferrous and ferric iron. Is capable of dissolving colloidal and
particulate Iron and thus gives a measure of total Iron content of the water.
METHOD
In the Dinko method Iron is reduced to the ferrous form and then reacted with 1,10-phenantroline to form an orange coloured complex.
A decomplexing agent is added into the reagent system in order to break down complexed forms of Iron. The test is simply carried out
by adding tablet reagents to a sample of the water under test.
The intensity of the colour produced is proportional to the Iron concentration and is measured using a Dinko Photometer.

Iron MR nº 1 Tablets / Iron MR nº 2 Tablets / Citrate IR Tablets
Round cuvette 10 mm path light. (4pcs). Code 1.9365.00.(cuvette used in the chart)
DINKO D-101 Photometer use calibration chart / DINKO D-105 Photoanalyzer and D-100 Photometer select program nr. 38.
PROCEDURE
1. – Fill the test tube with sample to the 10 ml mark.
2. – Add one Iron MR nr. 1 tablet, crush and mix to dissolve.
3. – Add one Iron MR nr. 2 tablet, crush and mix to dissolve.
4. – Stand for 10 minutes to allow full colour development.
5. – Select the filter 520 nm. on the Photometer..
6. – Take Photometer reading in usual manner. Make zero with sample without tablets.
7. – Consult Iron calibration chart(D-101). Select program nr. 38 (D-100 and D-105).
INTERFERENCES
Hardness 500 mg/l CaCO3, silica 150 mg/l SiO2 and Copper 3 mg/l Cu do not interfere with the test. Chromium 10mg/l may cause
slightly high results.
Nitrite greater than 50 mg/l NO2 causes low results and molybdates at any concentration causes precipitation. The pre-treatment
procedures described below using Citrate IR tablets remove interferences from nitrite up to 500 mg/l NO2 and molybdate up to 20 mg/l
MoO4. This pre-treatment does however reduce the tolerance to Chromium and is not recommended therefore for Chromium containing
samples.
Pre-treatment procedure using Citrate IR tablets . (50pcs) Code 1.9443.01, not included
Sample containing nitrite:
1. - Fill the test tube with sample to 10 ml mark.
2. - Add one Citrate IR tablet, crush and mix to dissolve. Ensure all particles are dissolved.
3. - Continue the test as described in the test procedure from stage 2 above but allow the tube to stand for 15 minutes to allow full
development before taking the photometer reading.
Sample containing molybdate:
1. - Fill the test tube with sample to 10 ml mark.
2. - Add one Iron nr. 1 tablet, crush and mix to dissolve
3. - Add one Citrate IR tablet, crush and mix to dissolve. Ensure all particles are dissolved.
4. - Continue the test as described in the test procedure from stage 3 but allow the tube to stand 15 minutes to allow full colour
development before taking the reading.
27
Range: 0- 5,0 mg / L Fe Iron 520 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.03 0.11 0.18 0.26 0.33 0.41 0.48 0.56
0.1 0.64 0.71 0.79 0.86 0.94 1.02 1.11 1.20 1.29 1.39
0.2 1.48 1.57 1.66 1.75 1.84 1.94 2.03 2.11 2.20 2.28
0.3 2.37 2.45 2.54 2.62 2..71 2.79 2.88 2.97 3.06 3.17
0.4 3.27 3.38 3.48 3.58 3.69 3.79 3.90 4.00 4.11 4.22
0.5 4.32 4.43 4.54 4.65 4.75 4.86 5.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,128mg/L Fe
Traceability: The traceability of Phenantroline method settles down with A.E. Harvery, Anal. Chem., 27,26(1955)
IRON. Code 1.9438.00 (50t) Photometer Method 577 - 580 nm

Test for high levels of Iron in natural and treated water. 0 - 10 mg / l
Iron occurs widely in nature and is found in many natural and treated waters. Iron is an objectionable constituent in both domestic and
industrial water supplies. Iron affects the taste of beverages and causes unsightly staining of laundered clothes, plumbing fittings,
swimming pool surfaces and the like. The formation of insoluble iron deposits is troublesome in many industrial applications and in
agricultural waters uses such as drip feed irrigation. In industry iron salts occur through corrosion of plant and equipment, and from
industrial processes.
Iron is therefore an important test for the monitoring of waters. The DINKO Iron test provides a simple method for determination of high
levels of iron in water over the range 0 to 10 mg/ l. The test responds to both ferrous and ferric Iron and thus gives a measure of the
total Iron content of the water.
METHOD
The DINKO Iron test is based on a simple tablet reagent containing an alkaline thioglycollate. The test is carried out simply by adding a
tablet to a sample of the water under test. The thioglycollate reduces ferric Iron to ferrous Iron and this, together with any ferrous iron
already present in the sample, reacts to give a pink coloration.
The intensity of colour produced is proportional to the Iron concentration and is measured using a DINKO Photometer.

Iron HR Tablet.
Round cuvette 16 mm. Ø (4pcs). Code 1.9365.00. (cuvette used in the chart). Square cuvette 10 mm with cap.(100pcs). Code
1.9363.00
DINKO D-101 Photometer use the calibration chart. Filter 580 nm.
DINKO D-105 Photoanalyzer and D-100 Photometer select program nr.14.
PROCEDURE
2. - Add one Iron HR tablet, crush and mix to dissolve.
3. - Stand for 1 minute to allow full colour development.
4. - Select the filter 580 nm on Photometer (D-101 ). D-105 and D-100 photometers select program nr. 14
5. - Take Photometer reading(see Photometer instructions). Make zero whit sample without tablet.
6. - Consult Iron calibration chart( D-101). Select program nr. 14 (D-105 and D-100)
IRON COMPLEXES
The test colour development will normally be completed within one minute. Continued colour development after this time is indicative of
more strongly bound Iron complexes in the water. In such cases the test solution should be stood for a longer period, say 10-15
minutes, until colour development is complete.
In certain industrial applications strong complexing agents are added to act as corrosion inhibitors. Moreover some samples may
contain precipitated Iron complexes or particles of metallic iron. These samples will require pre-treatment by a standard laboratory
procedure if it is required to determine the total Iron content.
The usual method of pre-treatment is acidification-with or without boiling, depending on the nature of the sample.
To use the Dinko Iron test after such pre-treatment procedures, add the Iron HR tablet to the acidified sample, adjust to pH 6,0-9,0 using
Ammonia or Sodium hydroxide, then take the photometer reading in the normal manner.
Range: 0 -10 mg / l Fe Iron 580 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 0.30 0.65 1.00
0.1 1.35 1.65 2.00 2.30 2.60 2.90 3.20 3.50 3.80 4.05
0.2 4.15 4.30 4.45 4.55 4.70 4.80 4.95 5.05 5.20 5.30
0.3 5.45 5.55 5.70 5.85 5.95 6.10 6.25 6.35 6.50 6.65
0.4 6.80 6.90 7.05 7.20 7.35 7.45 7.60 7.75 7.90 8.00
0.5 8.15 8.30 8.45 8.55 8.70 8.85 8.95 9.10 9.25 9.40
0.6 9.50 9.65 9.80 9.95 10.0
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,213 mg/L Fe
Traceability:The traceability of Thioglycollate method settles down with H.W. Swank. Ind. Eng. Chem. , Anal. Ed., 10,7 10,7(1938)
28
MAGNESIUM. Code 1.9440.00 (50t) - 1.9487.00 (250t) Photometer Method 520 nm
Test for Magnesium in water 0- 100 mg / l
Magnesium is a widely occurring natural element and is found in most water supplies. Magnesium salts contribute to the hardness of
water and higher levels of magnesium will be found therefore in hard water areas. Scale formation in heating and steam raising
equipment is promoted by the presence of magnesium salts in the water. Magnesium salts do however have a lower scale forming
tendency than calcium salts.
The DINKO Magnesium test provides a simple means of measuring magnesium l in water over the range 0-100 mg/l Mg.
METHOD
The DINKO Magnesium test is based on a simple colorimetric procedure. Magnesium reacts with an organic reagent to produce an
orange coloured complex. The reagent itself is yellow and thus over the range of the test a series of colours from yellow through to
orange are produced. The colour produced in the test is indicative of the magnesium concentration and is measured using a DINKO
Photometer.

Magnecol Tablet / Measuring Syringe 1ml
Round cuvette 16 mm Ø with cap.( 4pcs ). Code 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use calibration chart.
DINKO D-105 Photoanalyzer and D-100 Photometer select program nr.15.
PROCEDURE
1. - Using the measuring syringe take a 1 ml sample of the water under test. Transfer to the round test tube and make up to 10 ml mark
with deionised water.
2. - Add one Magnecol Tablet, crush and mix to dissolve.
3. - Stand for five minutes to allow full colour development and the slight turbidity to clear. Do not disturb the sample.
4. - Select the filter 520 nm on photometer.
5. - Take photometric reading (see Photometer instructions). Make zero with sample without tablet.
6. - Consult the Magnesium calibration chart(D-101). Select program nr. 15 (D-105 and D-100).
Range: 0-100 mg / L Mg Magnesium 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.1 0.0 0.8 1.9 3.0 4.1 5.2 6.3 7.4 8.5 9.6
0.2 10.7 11.8 12.9 14.0 15.1 16.2 17.3 18.4 19.5 21
0.3 22 23 24 26 27 28 29 31 32 33
0.4 34 36 37 38 39 41 42 43 45 46
0.5 47 48 50 51 52 54 55 56 57 59
0.6 60 62 64 66 68 70 72 74 76 78
0.7 81 84 87 90 94 97 100
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 2,835 mg/L Mg
Traceability:The traceability of method settles down with “SM”
MANGANESE. Code 1.9441.00 (50t) - 1.9488.00 (250t) Photometer Method 620 – 630 nm
Test for Manganese in water 0- 0,030 mg/l
Manganese salts are commonly found in many natural waters and is an objectionable constituent in water used for domestic purpose or
industrial applications. Manganese will cause brown or black staining to laundry or plumbing fittings even at very low concentrations. In
paper manufacturing or textile finishing similar staining can occur. Manganese salts may impart an astringent taste to drinking water
supplies, and in swimming pool applications can give an aesthetically displeasing brown coloration to the water.
The DINKO test provides an extremely sensitive method of measuring low concentrations of Manganese over the range 0 - 0,030 mg/l.
METHOD
Manganese may occur in water in various different valence states. In the first stage of method, manganese in lover valence states is
oxidised to form permanganate by the action of an oxidising agent. In the second stage the permanganate formed is further reacted with
leucomalachite green to form an intense turquoise coloured complex. Catalyst and inhibitors are incorporated into the tablet reagents to
ensure that colour reaction proceeds correctly and interferences are eliminated.
The intensity of colour produced in the test is proportional to the total Manganese concentration and is measured using a DINKO
Photometer.

Nr. 1 Tablet / Nr. 2 Tablet / Round cuvette 16 mm Ø with cap. (4pcs ). Code 1.9365.00. ( cuvette used in the chart)
Square cuvette 10 mm with cap. (100pcs). Code: 1.9363.00 / DINKO D-101 Photometer use the calibration chart. Filter 620nm
DINKO D-105 Photoanalyzer and D-100 select program nr. 39.
29
SAMPLE COLLECTION
Manganese is readily absorbed on to the surfaces of sample containers. To avoid loss of manganese test sample as soon as possible
after collection. It is important, because of extreme sensitivity of this test, to ensure that glassware used for the sample collection and
test procedure is very clean. For most accurate results in laboratory use it is recommended that all glassware is acid-rinsed and
thoroughly washed out with deionised water before re-use.
PROCEDURE
1. - Fill the test tube to the 10 ml mark with the sample (see Note 1).
2. - Add one Manganese nr. 1 tablet, crush and mix to dissolve.
3. - Add one Manganese nr. 2 tablet, crush and mix to dissolve then cap the tube.
4. - Stand for 20 minutes to allow full colour development (see Note 2)
5. - Select wavelength 620 nm. on the Photometer D-101. Select program nr. 39 on the D-100 and D-105 photometers.
6. - Take Photometer reading immediately(see Photometer Instructions). Make zero with sample without tablets.
7. - Consult Manganese calibration chart ( D-101). Select program nr. 39 (D-105 and D-100)
Notes
1.- Colour formation is extremely sensitive to temperature. The sample temperature should be about 20ºC for optimum test results.
2.- It is important to observe the standing period of 20 minutes ± 1 minute for optimum test results.
Any continuing colour development or colour change after this period should be ignored.
Range: 0- 0,030 mg / L Mn Manganese 620 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.1 0.000 0.000 0.001 0.001 0.001 0.001
0.2 0.002 0.002 0.002 0.002 0.003 0.003 0.003 0.003 0.004 0.004
0.3 0.004 0.004 0.005 0.005 0.005 0.005 0.006 0.006 0.006 0.006
0.4 0.007 0.007 0.007 0.008 0.008 0.008 0.009 0.009 0.009 0.010
0,5 0.010 0.010 0.011 0.011 0.011 0.012 0.012 0.012 0.012 0.013
0.6 0.013 0.013 0.013 0.014 0.014 0.014 0.015 0.020 0.020 0.024
0.7 0.026 0.027 0.030
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,002 mg/L Mn
Traceability: The traceability of the leucomalachita method settles down with Dr. A T Palin
MANGANESE. Code 1.9447.00 (50t) PhotometerMethod 550nm

Test for soluble Manganese in water 0 – 5 mg/l
Manganese-containing minerals occur widely and manganese salts are commonly found in many natural waters. Manganese is an
objectionable constituent in water used for domestic purposes or industrial applications. In domestic situations, manganese will cause
brown or black staining to laundry or plumbing fittings even at very low concentrations. In process applications such as paper
manufacturing or textile finishing similar staining can occur. Manganese salts may impart an astringent taste to drinking water supplies,
and in swimming pool applications can give an aesthetically displeasing brown coloration to the water.
In most cases where manganese salts occur naturally in the water, it will be necessary to apply special methods of removal before the
water can be used for domestic or industrial purposes. The Palintest Manganese test provides an extremely sensitive method of
measuring low concentrations of manganese for the assessment of natural waters and the control of manganese removal plant. The test
measures total manganese over the range 0 - 5 mg/l.
METHOD
Manganese may occur in water in various different valency states. This method offered is the Formaldoxime Method, with a range of 0
to 5,0mg/l Mn. In alkaline solution manganese reacts with formaldoxime to form an orange-red complex. The color developed is
proportional to the manganese concentration. Iron II ions also form a coloured complex, which will interfere.
The intensity of colour produced in the test is proportional to the total manganese concentration and is measured using a DINKO
Photometer.
Manganese HR Nr.1 Tablets /Manganese HR Nr. 2 Tablets /DINKO Photometer/ Round Test Tubes,10 ml glass (4pcs).Code 1.9365.00
SAMPLE COLLECTION
Manganese is readily absorbed onto the surfaces of sample containers. To avoid loss of manganese test sample as soon as possible
after collection.
It is important, because of the extreme sensitivity of this test, to ensure that glassware used for the sample collection and test procedure
is scrupulously clean. For most accurate results in laboratory use it is recommended that all glassware is acid-rinsed and then
thoroughly washed out with deionised water before use.
TEST PROCEDURE
Fill test tube with sample to the 10 ml mark
Add one Manganese HR nr. 1 tablet, crush and mix to dissolve.
Add one Manganese HR nr. 2 tablet, crush and mix to dissolve then cap the tube.
Stand for 5 minutes to allow colour development
Select Mn high, program nr. 63 on Photometer D-100 and D-105
Take Photometer reading in usual manner (see Photometer instructions).Make zero with sample without tablets.
The result is displayed as mg/l. Select the 550 nm filter.
Using the calibration chart( Photometer D-101), convert the absorbance reading into a mg/l Mn reading.
30
Range: 0-5 mg/L Mn Manganese 550 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.1 0.06 0.12 0.18 0.24 0.30 0.36 0.42 0.48 0.55
0.2 0.61 0.67 0.74 0.80 0.86 0.92 0.99 1.05 1.11 1.18
0.3 1.89 1.96 2.02 2.09 2.16 2.23 2.30 2.36 2.43 2.50
0.4 2.57 2.64 2.70 2.78 2.85 2.92 2.99 3.07 3.14 3.21
0.5 3.28 3.36 3.43 3.50 3.57 3.65 3.72 3.79 3.86 3.94
0.6 4.01 4.08 4.15 4.23 4.30 4.37 4.42 4.52 4.59 4.66
0.7 4.73 4.80 4.88 4.95
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,099 mg/L Mn
Traceability: The traceability of the Formaldoxima method settles down with A. Gottlieb and F.Hecht, Mikrochemie, 35, 337 (1950)
MOLYBDATE. Code 1.9442.00 (50t) Photometer Method 415- 420 nm

Test for low levels of molybdate in industrial waters and effluents 0 - 20 mg / l MoO4
Formulations containing molybdate are used as corrosion inhibitors in industrial water treatment like cooling systems. Molybdate based
formulations have replaced older forms of corrosion inhibitors. When using molybdate treatment it is necessary to control the molybdate
concentration within specified levels depending on the application involved. Moreover, since molybdates are widely used in water
treatment and industrial process, molybdate is an increasingly important test for effluents and industrial discharges.
The DINKO molybdate test provides a simple means of measuring low levels of molybdate in industrial waters and effluents and covers
the range 0-20 mg / l MoO4 ( 0-12 mg / l Mo ).
METHOD
Molybdates react with dihydroxybezen disulphonic acid salt under slightly acid conditions to give a yellow coloured complex. Under the
conditions of the test, iron does not interfere and there is no significant interference from other metals at levels likely to be found in
industrial water systems(see note). The reagents are provided in the form of two tablets for maximum convenience. The test is simply
carried out by adding one of each tablet to a sample of water. The intensity of the colour produced in the test is proportional to the
molybdate concentration and is measured using a DINKO Photometer.

Molybdate nr. 1 LR Tablet / Molybdate nr. 2 LR Tablet
Round cuvette 16 mm. Ø (4pcs). Code 1.9365.00 (cuvette used in the chart) / Square cuvette 10 mm. Pk. (100). Code 1.9363.00
DINKO D-101 Photometer use calibration chart. Filter 420nm.
DINKO D-105 Photoanalyzer and D-100 Photometer, select program nr. 16.
PROCEDURE
3. - Add one Molybdate nr. 1 LR tablet, crush and mix to dissolve.
4. - Add one Molybdate nr. 2 LR tablet, crush and mix to dissolve.
6. - Select filter of 420 nm on the Photometer D-101 and select program nr. 16 with D-105 and D-100 photometers
7. - Take Photometer reading in the usual manner. Make zero with sample without tablets.
8. - Consult the Molybdate calibration chart(D101). Select program nr.16(D-105 and D-100).
Molybdate concentrations can be expressed in a number of different ways.

To convert from MoO4 to Na2MoO4 multiply by 1,3 / To convert from MoO4 to Mo multiply by 0,6
INTERFERENCES
1. - Copper, Zinc, Calcium and phosphate do not interfere at levels up to 20 mg/l.

2. - Iron and Chlorine at levels of 10mg/l cause slightly high blank readings (equivalent to 0.6 mg/l MoO4). They do not, however, cause
any interference when in the presence of molybdate.
31
Range: 0 - 20 mg / L MoO4 Molybdate 420 nm
ABS 0 5 ABS 0 5 ABS 0 5
0.02 0.00 0.22 6.80 7.00 0.42 13.6 13.8
0.03 0.10 0.27 0.23 7.15 7.35 0.43 13.9 14.1
0.04 0.47 0.62 0.24 7.50 7.70 0.44 14.3 14.5
0.05 0.79 0.96 0.25 7.90 8.05 0.45 14.6 14.8
0.06 1.13 1.30 0.26 8.20 8.40 0.46 15.0 15.2
0.07 1.47 1.64 0.27 8.55 8.70 0.47 15.4 15.6
0.08 1.81 1.98 0.28 8.85 9.00 0.48 15.7 15.9
0.09 2.15 2.32 0.29 9.20 9.35 0.49 16.1 16.3
0.10 2.50 2.67 0.30 9.50 9.70 0.50 16.5 16.6
0.11 2.84 3.01 0.31 9.85 10.0 0.51 16.8 17.0
0.12 3.18 3.35 0.32 10.2 10.3 0.52 17.2 17.4
0.13 3.52 3.70 0.33 10.5 10.7 0.53 17.6 17.8
0.14 3.86 4.04 0.34 10.8 11.0 0.54 18.0 18.2
0.15 4.22 4.40 0.35 11.2 11.3 0.55 18.3 18.5
0.16 4.59 4.77 0.36 11.5 11.6 0.56 18.7 18.9
0.17 4.95 5.15 0.37 11.8 12.0 0.57 19.1 19.3
0.18 5.30 5.50 0.38 12.1 12.3 0.58 19.5 19.7
0.19 5.70 5.85 0.39 12.5 12.7 0.59 19.8 20.0
0.20 6.05 6.25 0.40 12.9 13.0
0.21 6.40 6.60 0.41 13.2 13.4
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,567 mg/L MoO4
Traceability: The traceability of Tiron method settles down with J. H. Yoe, Anal. Chem. y F. Will, Anal. Chim-Acta, 8,546(1953)
MOLYBDATE. Code 1.9446.00 (50t) - 1.9480.00 (250t) Photometer Method 415 - 420 nm
Test for molybdate in industrial water. 0 - 100 mg / l MoO4
Formulations containing molydate are used as corrosion inhibitors in industrial water treatment. When using molybdate treatment it is
necessay to control the molybdate concentration.
METHOD
The molybdate react with thioglycollate under acid conditions to give a yellow coloured complex. Oxidising conditions are maintained
during the acidification stage in order to keep the molybdate in a fully oxidised state. Under the conditions of the test, Iron does not
interfere and there is no significant interference from other metals at levels likely to be found in industrial water systems. The reagents
are provided in the form of two tablets for maximum convenience. The test is simply carried out by adding one of each tablet to a
sample of water.
The intensity of the colour produced in the test is proportional to the molybadte concentration, and is measured with a DINKO
Photometer.

Molybdate nº 1 HR Tablet / Molybdate nº 2 HR Tablet
Round cuvette 16 mm.Ø (4pcs). Code 1.9365.00 (cuvette used in the chart) / Square cuvette 10 mm. (100pcs). Code 1.9363.00
DINKO D-105 Photoanalyzer and D-100 Photometer, select program nr. 40.
PROCEDURE
2. - Add one Molybdate nr. 1 HR tablet, crush and mix to dissolve.
3. - Add one Molybdate nr. 2 HR tablet, crush and mix to dissolve.
4. - Select wavelength 420 nm on the Photometer D-100. Select program nr. 40 (D-100 and D-105)
5. - Take Photometer reading in the usual manner. Make zero with sample without tablets.
6. - Consult the Molybdate calibration chart, Photometer D-101. Select program nr.40, D-105 and D-100 Photometers.
Molybdate concentrations can be expressed in a number of different ways.

To convert from MoO4 to Na2MoO4 multiply by 1,3
To convert from MoO4 to Mo multiply by 0,6
Range: 0 - 100 mg / L MoO4 Molybdate 420 nm

ABS 0 1 2 3 4 5 6 7 8 9
0,0 0.0 0.8 2.1 3.3 4.5
0,1 5.7 6.9 8.1 9.3 10.6 11.8 13.0 14.2 15.4 16.6
0,2 17.8 19.0 20 22 23 24 25 27 28 29
0,3 30 32 33 34 35 37 38 39 41 43
0,4 44 46 48 50 52 53 55 57 59 61
0,5 62 64 66 68 69 71 73 75 76 78
0,6 80 82 83 85 87 89 91 93 95 97
0,7 100
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 2,835 mg/L MoO4
Traceability: The traceability of the method settles down with “Analytical Chemestry”, 25(9) 1363(1953)
32
NICKEL. Code 1.9448.00 (50t) - 1.9490.00 (200t) Photometer Method 520 nm
Test for Nickel in natural and treated waters. 0 - 10 mg / l Ni
Nickel does not occurs naturally in waters but only as result from industrial process in coating and steel industries. The EC maximum
admissible concentration for drinking water( MAC ) is 0.05 mg / l.
The DINKO method provide a simple test for Nickel over the range of 0 - 10 mg / l Ni. The test determine Ni2+ and Ni4+ and the result
represent total soluble inorganic Nickel concentration in the sample.
METHOD
In the DINKO method, the Nickel salts are reduced to Nickel (II), and react with Nioxim indicator to give purple coloured complex. The
reagents are provided in tablet form and powder reagent to eliminate Copper and Iron interferences. The intensity of colour produced in
the test is proportional to the Nickel concentration and is measured using a DINKO Photometer.

Nickeltest PR Powder(spoon pack) / Nickeltest nr. 1 Tablet / Nickeltest nr. 2 tablet
Round cuvette 16mm.Ø (4pcs). Code 1.9365.00 (cuvette used in the chart) / Square cuvette 10 mm. (100pcs). Code 1.9363.00
DINKO D-101 Photometer use the calibration chart / DINKO D-105 Photoanalyzer and D-100 select program nr. 17.
PROCEDURE
1. - Fill the test tube to the 10 ml mark with the sample.
2. - Add one Nickeltest nr. 1 tablet, crush and mix to dissolve. Ensure all particles are dissolved.
3. - If iron is present add one level spoonful of Nickeltest PR powder and mix to dissolve.
4. - Add one Nickel nr. 2 tablet, crush and mix to dissolve.
5. - Stand for 2 minutes to allow full colour development. Select 520 nm filter on the Photometer.
6. - Take Photometer reading (see Photometer Instructions). Make zero with sample without tablets.
7. - Consult Nickel calibration chart D-101 Photometer. Select program nr. 17, Photomters D-105 and D-100.
Range: 0 - 10 mg / L Ni Nickel 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.11 0.23 0.35 0.47 0.59 0.71 0.83 0.95 1.07
0.1 1.19 1.30 1.42 1.54 1.66 1.78 1.90 2.02 2.15 2.28
0.2 2.41 2.54 2.67 2.80 2.93 3.06 3.19 3.32 3.45 3.58
0.3 3.71 3.84 3.97 4.11 4.25 4.39 4.53 4.67 4.81 4.95
0.4 5.09 5.23 5.38 5.52 5.66 5.80 5.94 6.08 6.21 6.35
0.5 6.48 6.62 6.75 6.89 7.02 7.16 7.29 7.43 7.56 7.70
0.6 7.83 7.97 8.12 8.27 8.43 8.58 8.73 8.89 9.04 9.20
0.7 9.35 9.50 9.66 9.82 10.00
Notes
1. - The presence of Cobalt at 0,5mg / l gives a positive response in the test.
2. - The presence of significant levels of EDTA ( at least 25 mg / l ) complexes Nickel and reduces response in the test.
Complexing agents used in water treatment, such as polyphosphates, do not affect the results.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,198mg/L Ni
Traceability: The traceability of the method settles down with “SM” Standard Methods
NITRATE. Code 1.9450.00 (50t) - 1.9491.00 (200t) Photometer Method 577 - 580 nm
Test for nitrate in natural, drinking and waste water. 0 - 1 mg / l N
Nitrates enter water supplies from the breakdown of natural vegetation, the use of chemical fertilisers in modern agriculture and from the
oxidation of nitrogen compounds in sewage effluents and industrial wastes.
Nitrate is an important control test for water supplies. Drinking waters containing excessive amounts of nitrates can cause
methaemoglobinaemia in bottle-fed infants(blue babies). The EEC has set a recommended maximum of 5,7 mg/ l N (25 mg / l NO3) and
an absolute maximum of 11,3 mg/l N (50 mg/l NO3). The DINKO Nitrate method provides a simple test for nitrate nitrogen over the
range 0-1 mg / l N. The test can however be extended to cover the range 0 -20 mg / l by simple dilution technique.
METHOD
In the Nitrate test method nitrate is first reduced to nitrite, the resulting nitrite is then determined by a diazonium reaction to form a
reddish dye. The reduction stage is carried out using the unique zinc-based Nitratest Powder, and Nitrates Tablet which aids rapid
flocculation after the one minute contact period. The test is conducted in a special Nitrates Tube –a graduated sample container with
hopper bottom to facilitate settlement and decanting of the sample.
The nitrite resulting from the reduction stage is determined by reaction with sulphanilic acid in the presence of N-(1-naphtyl)-ethylene
diamine to form a reddish dye. The reagents are provided in a single Nitricol tablet which is simply added to the test solution.
The intensity of the colour produced in the test is proportional to the nitrate concentration and is measured using a Dinko Photometer.

Nitratest Powder (Spoon Pack) / Nitratest Tablet) / Nitricol Tablet / Tube 20 ml mark. Code 1.9450.01
Round cuvette 16 mm. Ø (4pcs).Code 1.9365.00 / DINKO Photometer D-101. Use the calibration chart
DINKO D-105 Photoanalyzer and D-100 select program nr.18.
33
PROCEDURE
1.- Fill the Tube with sample to the 20 ml mark.
2.- Add one level spoonful of Nitratest Powder and one Nitratest tablet. Do not crush the tablet. Replace screw cap and shake tube well
for one minute.
3.- Allow tube to stand for about 1 minute then gently invert three or four times to aid flocculation. Allow tube to stand for 10 minutes
to ensure complete settlement. Take care to avoid transferring any Nitratest Power. Ideally, transfer the liquid using a pipette.
4.- Remove screw cap and wipe around the top of the tube with a clean tissue. Careful decant the clear solution into a round test tube,
filling to the 10ml mark.
5.- Add one Nitricol tablet, crush and mix to dissolve.
6.- Stand for 10 minutes to allow full colour development.
7.- Select filter 580 nm on Photometer D-101 . Select program nr. 18 with D-100 and D-105 photometers.
8.- Take Photometer reading (see Photometer instructions). Make zero with sample without tablets.
9.- Consult Nitrate calibration chart, Photometer D-101. Select program nr.18, Photometers, D-105 and D-100.
Concentrations of nitrate greater than 1,0 mg/l may be determined by diluting the original sample with deionised water. The test can be
conveniently carried out over a range 0 - 20mg / l N as follows:
Take a clean Tube. Add 1ml of sample using a pipette or graduated dropper. Fill the Tube to 20 ml mark with deionised water. Continue
the test procedure as given in steps 2 to 9 above. Multiply the chart reading obtained by 20 to obtain the nitrate concentration in the
original sample.
NITRITE CORRECTION
The Nitrate test method will also respond to any nitrite present in the sample. In most natural and drinking waters the amount of nitrite
will be small in comparison to the nitrate concentration. If it is desired to correct for nitrite, determine nitrite concentration (as mg/l N) in
the prescribed manner(see Nitrite Test) and deduct from the nitrate concentration (as mg/l N) obtained from the Nitrate Test.
Range: 0-1 mg / L N 580 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.000 0.009 0.017 0.026 0.034 0.043 0.051
0.1 0.060 0.068 0.077 0.085 0.094 0.103 0.113 0.123 0.133 0.143
0.2 0.153 0.163 0.173 0.183 0.193 0.203 0.213 0.223 0.233 0.243
0.3 0.253 0.263 0.273 0.283 0.293 0.303 0.315 0.327 0.339 0.351
0.4 0.363 0.375 0.387 0.400 0.412 0.424 0.436 0.448 0.460 0.472
0.5 0.484 0.496 0.508 0.520 0.532 0.544 0.556 0.568 0.580 0.592
0.6 0.606 0.621 0.636 0.651 0.667 0.682 0.697 0.712 0.728 0.743
0.7 0.758 0.774 0.789 0.805 0.822 0.840 0.857 0.874 0.892 0.909
0.8 0.926 0.944 0.961 0.979 1.000
To convert mg/L of N to mg/L of NO3 multiply result by 4,4
Traceability: The traceability of the method of reduction settles down with “SM” Standard Method 4500-NO3
NITRATE. Code 1.9010.00 (25t) Photometer Method 415/420 nm

Test for nitrate in drinking and waste water. 0 - 30 mg / l N
0 - 150 mg / l NO3
Nitrates are normally present in natural, drinking and waste waters. Nitrates enter water supplies from the breakdown of natural
vegetation, the use of chemical fertilisers’ in modern agriculture and from the oxidation of nitrogen compounds in sewage effluents and
industrial wastes.
Nitrate is an important control test for water supplies. Drinking waters containing excessive amounts of nitrates can cause
methaemoglobinaemia in bottle-fed infants(blue babies). The EEC has set a recommended maximum level of 5,7 mg/ l N (25 mg/ l NO3)
and an absolute maximum of 11,3 mg/l N (50 mg/l NO3) for nitrate in drinking water.
The DINKO method provides a simple test for nitrate over the range 0 - 30 mg/ l N (0 - 150 mg/ l NO3).
This test is also used in the colour development stage of Total Nitrogen test code 1.9012.00.
MÉTHOD
In this metohd, nitrate reacts with chromotropic acid, under strongly acid conditions to produce a yellow colour. Chemicals are
incorporated to prevent interference from nitrite, chloride, Iron(Fe III), Chlorine and other oxidising agents. The reagents are provided in
the form of a predispensed tube and a powder. The test is simply carried out by adding a sample of the water and a scoop of powder to
a tube.
The intensity of colour produced in the test is proportional to the nitrate concentration and is measured using Dinko Photometer.
WORKING PRACTICE
This method should be carried out in concordance with Good Laboratory Practice. The reagent tubes contain 90% Sulphuric Acid and
must be handled with care. The use of appropriate protective clothing, gloves and safety spectacles is recommended. In the even of
skin or eye contact, or spillage, wash immediately with large amounts of water.
Particular care should be taken when opening the reagents tubes to add the water sample as heat will be produced and gases may be
evolved. Samples containing cyanide or sulphides will release toxic fumes and for such samples the test must always be carried out in a
fume cupboard. It is generally recommended that the test be conducted in a fume cupboard where available.
34
Reagents tubes / Nitrate Powder / Pipettor 1ml / Dosing Scoop / Dossing Funnel / Dossing Scoop Scraper
Photometer D-101, calibration chart, filter 420nm / Photoanalyzer D-105 and D-100, program nr. 60.
Use of Dosing Scoope and Funnel
This elements are specially designed to ensure accurate dosing of reagent powders into the tube-tests.
1. - Dip the scoop into the powder and ensure that it is completely filled. Draw the scraper across the top of the scoop to ensure a level
fill.
2. - Place the funnel on top of the tube-test. Locate the scoop in the groove on the side of the funnel. Rotate the scoop to invert then tap
gently to ensure that all the reagent goes into the tube.
PROCEDURE
1.- Remove the cap of tube-test and add 1,0 ml of sample using a pippetor. DO NOT SHAKE THE TUBER.
2.- Add one level scoop of Nitrate Powder using a dosing scoop. Cap tube and gently invert five or six times to dissolve and mix the
reagents and sample.
3.- Stand for five minutes to allow colour development.
4.- Select 420 nm filter, Photometer D-101. Select program nr. 60 in the D-105 Photoanalyzer and D-100 Photometer
5.- Take Photometer reading. Make the zero with sample without reagents or use an unused tube-test.
6.- Consult the calibration chart, Photometer D-101. Select program nr. 60, D-105 and D-100 Photometers.
Interferences
The test system incorporates reagents to prevent potential interferences from nitrite, chloride, Iron(Fe III), and Chlorine and other
oxidising agents. Interferences studies have shown that levels up to nitrite 10 mg/l, chloride 1000 mg/l, Iron 40 mg/l and Chlorine 5 mg/l
do not affect the result of the test.
Notes
Nitrate tube-tests is light sensitive. Store in original pack and keep lid closed when not in use. The tubetests contents must be disposed
of in accordance with waste regulations and the laid-down disposal procedures of the laboratory of use.
Range: 0-30 mg / L N 420 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.00 0.10 0.00 0.22 0.49 0.77 1.04 1.31 1.59
0.1 1.86 2.13 2.41 2.68 2.95 3.23 3.5 3.8 4.0 4.3
0.2 4.6 4.9 5.1 5.4 5.7 6.0 6.2 6.5 6.8 7.1
0.3 7.3 7.6 7.9 8.2 8.4 8.7 9.0 9.2 9.5 9.7
0.4 9.9 10.2 10.4 10.7 10.9 11.2 11.4 11.6 11.9 12.1
0.5 12.4 12.6 12.9 13.1 13.3 13.6 13.8 14.1 14.3 14.6
0.6 14.8 15.0 15.3 15.5 15.8 16.0 16.3 16.5 16.7 17.0
0.7 17.2 17.5 17.7 18.0 18.2 18.6 18.9 19.2 19.5 19.8
0.8 20.1 20.4 20.7 21.0 21.3 21.6 22.0 22.3 22.6 22.9
0.9 23.2 23.5 23.8 24.1 24.4 24.8 25.1 25.4 25.7 26.0
1.0 26.3 26.6 27.0 27.3 27.6 27.9 28.2 28.5 28.8 29.2
1.1 29.5 29.8
To convert mg/ L of N to mg/ L of NO3 multiply the result by 4,4
Traceability: The traceability of the method of reduction settles down with “SM” Standard Methods
NITRITE. Code 1.9454.00 (50t) - 1.9492.00 (250t) Photometer Method 520 nm

Test for nitrite in drinking and waste water. 0- 0,5 mg / l N (0 -1,6 mg / l NO2)
Nitrite are found in natural waters as an intermediate product in the nitrogen cycle. Nitrite is harmful to fish and other forms of aquatic life
and should be controlled in waters used for fish farms and aquariums. The nitrite test is also applied for pollution control in waste waters
and drinking water. The DINKO Nitrite test provides a simple method of measuring Nitrogen levels over the range 0 to 0.5 mg / l N.
Higher levels can be determinate by diluting the sample.
METHOD
Nitrites in acid solution react with sulphanilic acid producing a diazo compound that couples with N-(1-naphthyl)ethylenediamine to form
a reddish dye. The test is simply carried out adding a tablet containing both of these reagents in acidic formulation to a sample of the
water under test. The intensity of the colour produced in the test is proportional to the nitrite concentration and is measured using a
DINKO Photometer.

Nitricol Tablet / Round cuvette 16mm Ø with cap. (4pcs ). Code: 1.9365.00. (cuvette used in the chart)
DINKO D-101 Photometer use calibration char/ DINKO D-105 Photoanalyzer and D-100 Photometer select program nr. 19.
PROCEDURE
1. - Fill round test tube with sample to the 10 ml mark.
2. - Add one Nitricol Tablet, crush and mix to dissolve.
4. - Select wavelength 520nm on Photometer.
5. - Take Photometer reading (see photometer instructions). Make zero with sample without tablets
6. - Consult Nitrite calibration chart (D-101). Select program nr.19 (D-105 and D-100)
35
Range: 0- 0,5 mg / L N Nitrite 520 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.000 0.003 0.007 0.010 0.014 0.017 0.021 0.025 0.028
0.1 0.032 0.035 0.039 0.042 0.046 0.050 0.053 0.057 0.060 0.064
0.2 0.067 0.071 0.075 0.078 0.082 0.085 0.089 0.092 0.096 0.100
0.3 0.103 0.107 0.111 0.115 0.118 0.122 0.126 0.130 0.134 0.137
0.4 0.141 0.145 0.149 0.153 0.156 0.160 0.164 0.168 0.171 0.175
0.5 0.179 0.183 0.187 0.190 0.194 0.198 0.202 0.205 0.209 0.212
0.6 0.216 0.219 0.223 0.226 0.230 0.233 0.237 0.240 0.244 0.247
0.7 0.251 0.254 0.258 0.261 0.265 0.268 0.272 0.275 0.279 0.282
0.8 0.286 0.289 0.293 0.296 0.300 0.303 0.307 0.310 0.314 0.317
0.9 0.321 0.324 0.328 0.331 0.335 0.338 0.342 0.345 0.349 0.352
1.0 0.356 0.360 0.363 0.367 0.370 0.374 0.377 0.381 0.384 0.388
1.1 0.391 0.395 0.398 0.402 0.405 0.409 0.413 0.416 0.420 0.424
1.2 0.427 0.431 0.435 0.438 0.442 0.445 0.449 0.453 0.456 0.460
1.3 0.464 0.467 0.471 0.475 0.478 0.482 0.486 0.489 0.493 0.497
1.4 0.500
To convert from mg/L N to mg/L NO2 multiply result by 3,3.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,057mg/L N
Traceability: The traceability of the method of settles down with “USEPA”-Federal Register 4500-NO2
NITRITE. Code 1.9455.00 (50t) - 1.9501.00 (250t) Photometer Method 490 nm

Test for nitrite in cooling water 0 -1500 mg / l NaNO2
Nitrite and nitrite-based formulations are widely used for corrosion control in cooling water systems. The Dinko test provides a simple
means of measuring nitrite for the control of such treatment products in cooling water. The test covers the range 0-1500 mg/l NaNO2
MÉTHOD
The method is based on a colorimetric procedure using an iodide containing reagent system. Nitrites catalyse the oxidation of iodide to
iodine under mildly acid conditions to produce a brown coloration. Over the range of a test a series of colours through yellow to brown
are produced. The intensity of the colour produced in the test is proportional to the nitrite concentration and is measured using a Dinko
Photometer.

Nitriphot nº 1 Tablet / Nitriphot nº 2 Tablet / Syringe 1ml
Round cuvette 16 mm. Ø (4pcs). Code 1.9365.00 (cuvette used in the chart) / Square cuvette 10 mm. (100pcs). Code 1.9363.00
DINKO D-101 Photometer use the calibration chart / DINKO D-105 Photoanalyzer and D-100 select program nr. 41.
PROCEDURE
1. - Filter sample if necessary to obtain a clear solution. Using the syringe take 1 ml of sample and transfer to the test tube. Make up to
the 10 ml mark with deionised water.
2. - Add one Nitriphot nr. 1 tablet. Crush and mix to dissolve.
3. - Add one Nitriphot nr. 2 tablet. Crush and mix to dissolve. Cap tube immediately.
5. - Select the 490 nm filter on the Photometer.
6. - Take Photometer reading. Make zero with sample without tablets.
7. - Consult calibration chart, Photometer D-101. Select program nr. 41, D-105 and D-100 Photometers.
Range: 0 -1500 mg / L NaNO2 Sodium Nitrite 490 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 0 12 24 37 49 61 73 85 98
0.1 110 122 134 146 163 182 201 219 238 257
0.2 276 294 332 377 422 467 512 557 602 647
0.3 692 738 783 828 873 917 961 1004 1047 1090
0.4 1134 1177 1220 1263 1307 1350 1377 1404 1430 1456
0.5 1480 1500
Interferences:
Positive Chlorine in excess of 30 mg/l may give slight positive interference. However, nitrite and chlorine are incompatible and do not
normally co-exist.
The solution should be cooled to below 30ºC before testing for the most accurate analytical results.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of:
To 150 mg/L ± 17,01 mg/L NaNO2
From 150 to 300 mg/L ± 28,35 mg/L NaNO2
Traceability: The traceability of the method settles down with “SM” Standard Methods
36
NITROGEN TOTAL/30. Code1.9012.00 (25t) Photometer Method 415/420 nm
Test for Total Persulphate Nitrogen in natural and waste water. 0 - 30 mg / l N
Total nitrogen is a vital test for assessing the quality of effluents and waste water prior to discharge. In the UK the Urban Waste Water
Treatment Regulations(1994) make provision for control of discharge of total nitrogen to sensitive bodies of natural water. The
monitoring of rate of nitrogen removal is therefore of great importance in waste water treatment. Total nitrogen is composed of nitrate,
nitrite ammonium and organic nitrogen compounds. The DINKO “Test Tube” for Total Nitrogen provides a simple method of measuring
total persulphate nitrogen over the range of 0-30 ml/L N.
METHOD
The DINKO “Test Tube” Total Nitrogen test is a simple two stage procedure. The sample is initially digested with alkaline persulphate to
break down nitrogenous compounds which are then converted to nitrate. The digested sample is then transferred to a Dinko “Tube Test”
Nitrate tube for determination of total nitrogen present. The reagents are provided in the form of predispensed tubes and powders. The
powders are using a specially designed scoop and funnel.
The intensity of colour produced in the test is proportional to the total nitrogen concentration and is measured using a Dinko
Photometer.
In the total nitrogen determination, the recovery of different compounds depends to an extent on the method of oxidisation used the
make the conversion to nitrate. It is formal purpose. Results from the DINKO Total Nitrogen test should therefore be expressed as “Total
Persulphate Nitrogen”
WORKING PRACTICE
This method is a simplified laboratory procedure and should be carried out in accordance with good laboratory working practice.
The Total Nitrogen tubes contain sodium hydroxide solution, to wich potassium persulphate is added.The Tubetest Nitrate tubes contain
strong sulphuric acid. These reagents must be handled with care. The use of appropriate protective clothing, gloves and safety
spectacles is recommended. In the event of skin or eye contact, or spillage, wash inmediately with large amounts of water.
Particular care should be taken when adding Tubetests Total Nitrogen Reagent nº 2 to the digestion tubes. Sulphur dioxide will be
evolved. Care should be taken when opening the Tubetests Nitrate tubes which contains concentrated acid. On adding the digestate
head will be produced, the tube will become hot and gases may be evolved. It is generally recommended that the test be conducted in a
fume cupboard where available, particularly in the case of samples originally know to contain toxic materials such as cyanide or
sulphide.

Digestion stage
Tubetests Total Nitrogen Tubes Tubetests Total Nitrogen Reagent nº 1
Tubetests Total Nitrogen Reagent nº 2 Tube Heater D-64 or D-65
Pipettor 5 ml Dossing scoop size 1
Dossing scoop size 4 Dossing funnel
Dossing scoop scraper
Colour Development stage
Tubetests Nitrate Tubes Tubetests Nitrate Powder
Pipettor 1ml Dossing scoop size 1
Dossing funnel Dossing scoop scraper
Pipettor 1ml
DINKO D-105 Photoanalyzer and D-100 Photometer select the program nr. 61.
Use of Dosing Scoop and Funnel
This Tubetest method uses Dossing scoops and funnels. The scoopes and funnels are specially designed to ensure accurate dosing od
reagent powders into the Tubetests tubes.
1. Select the correct size scoop. Dip the scoop into the powder and ensure that it is completed filled. Draw the scraper across the top of
the scoop to ensure a level fill.
2. Place the funnel on top of the tubetest . Locate the scoop in the groove on the side of funnel.
Rotate the scoop to invert then tap gently to ensure that all the reagent goes into the tube.
Test Instructions-Digestion stage
1.Turn on the Tube heater, set the control to the 105ºC mark and allow to heat up to temperature.
2. Remove the cap of the Total Nitrogen Tube and add three level scoops of Total Nitrogen Reagent nº1 using the size 1 dosing scoop
and funnel.
3. Add 5.0 ml of sample using a pipettor. Replace the cap tightly and shake the tube vigorously for 30 seconds
4. Label the tube and place in the Tube Heater. Ensure the safety screen is in position and digest the tube for 30 minutes, then turn of
the heater.
5. Carefully remove each tube and transfer to a test tube rack. Handle hot tubes by the cap only.
6. Allow tubes to cool to room temperature.
7. Remove the cap of the Tubetests Total Nitrogen Tube and add one level scoop of Tubetets Total Nitrogen Reagent nº 2 using the
size 4 dosing scoop and funnel. Take care, sulphur dioxide will be evolved.
8. Cap the tube and shaker for 15 seconds, then stand for 3 minutes.
Test Instructions- Colour Development stage
1.Using a pipettor, transfer 1 ml of digested sample from the Tubetests Total Nitrogen Tube to a Tubetests Nitrate Tube.
Take care to add the digestate slowly. DO NOT SHAKE THE TUBE.
2. Add one level scoop of Tubetests Nitrate Powder using the size 1 dosing scoop and funnel. Cap tube and invert slowly ten times to
dissolve and mix the reagents and samples. Take care. The tube will become hot.
3. Stand for five minutes to allow colour development.
37
4. Select filter of 420 nm on Photometer D-101. Select program nr.61 on Photoanalyzer D-105 and Photometer D-100.
5. Take photometer reading in usual manner( see Photometer Instructions). Use an unused Tubetests Nitrate Tube to set the blank on
the Photometer.
6. Consult the Total Persulphate Nitrogen calibration chart. With the DINKO D-105 Photoanalyzer and D-100 Photometer select the
program nr. 61
Range: 0-30 mg / L Persulphate Nitrogen/30 N 420/415 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.0 0.0 0.1 0.1 0.2 0.2 0.3 0.6 0.9 1.3
0.1 1.6 1.9 2.3 2.6 2.9 3.3 3.6 3.9 4.3 4.6
0.2 5.0 5.4 5.8 6.2 6.6 7.0 7.4 7.8 8.2 8.6
0.3 9.0 9.4 9.8 10.2 10.6 11.0 11.4 11.8 12.2 12.6
0.4 12.9 13.3 13.7 14.1 14.5 14.9 15.4 15.8 16.2 16.6
0.5 16.9 17.3 17.7 18.1 18.5 18.9 19.3 19.7 20.1 20.5
0.6 20.9 21.4 21.8 22.2 22.7 23.1 23.5 23.9 24.3 24.8
0.7 25.2 25.6 26.1 26.5 26.9 27.3 27.8 28.2 28.6 29.0
0.8 29.5 29.9 30.3 30.5
To convert mg/ l de N to mg/ l NO3 multiply result by 4,4
Notes:
1-This method is based on the Persulphate Method from “Standard Methods for the Examination of Water and Waste Water” 19 th
Edition 1995 page 4-95. The method, in general, does not yield 100% recovery. Recoveries of various nitrogen compounds have
been tested in our laboratories. Inorganic compounds such as potassium nitrate, sodium nitrite and ammonium chloride yiels in
excess of 95% recovery. The typical recoveries of some organic nitrogen compounds are quoted below:
Compound Typical Recovery

Glycine 95% all levels
Urea 90% all levels
Nicotinic Acid 95% at 10 mg/l, 45% at 30 mg/l
Creatinine 100% at 10 mg/l, 70% at 30 ml/l
2- Tubetests Nitrate Powder is light sensitive. Store in original pack and keep lid closed when not in use.
3- Disposal. The used Tubetests Nitrate/30N Tubes contain strong sulphuric acid and other chemical reagents and care must therefore
be exercised in their disposal. The tube contents should be disposed of in accordance with the laid-down disposal procedures of the
laboratory of use.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0.567 mg/L N
Traceability: The traceability of the method of settles down with, “ Standard Methods” 4500-NO3 – Persulphate Method.
ORGANOPHOSPHONATE (OP ). Code 1.9414.00 (50t) - 1.9502.00 (250t) Photometer Method 620 - 630 nm
Test for organophosphonate in cooling water. 0 - 20 mg / l PO4
The use of organophosphonate compounds as inhibitors in cooling systems has become widespread and is essential to control the
concentration.
The DINKO OP test provides a reliable means of monitoring organophosphonate levels over the range 0-20 mg/l PO4. The test has
been developed for use with commercially available organophosphonate products such as those based on aminotrimethyl phosphonic
acid and hydroxyethane diphosphonic acid.
METHOD
In the OP test organophosphonate are first converted to orthophosphate by a catalysed cold oxidation process. Excess oxidising agent
is removed from the sample by precipitation and filtration. The orthophosphate formed in the reaction is then determined using the
“molybdenum blue” method. The reagents for the procedure are provided in tablet form and the test is simply carried out by adding the
appropiate tablets in sequence to a diluted sample of water.The intensity of blue coloration formed in the test is proportional to the
organophosphonate concentration and is determined using a DINKO Photometer.
A separate correction procedure is applied to those sample known or suspected to contain orthophosphate.

Oxidising OP Tablets OP-A Tablet
OP-B Tablet OP-AX Tablet
Tube, mark 20ml, code 1.9450.01 Round cuvette 16mm with cap.(4pcs) Code 1.9365.00
Filtration kit (syringe c.luer+10 syringe filter), code 1.9594.01 (order separate) DINKO D-101 Photometer use calibration chart.
DINKO D-105 and D-100 Photometers select program nr. 42.
SAMPLE PREPARATION AND DILUTION

1. - Filter sample if necessary to obtain a clear solution. Glass fiber filter can be used.
2. - Prepare x 5 dilution of sample.
This diluted sample is used for both the organophosphonate and correction procedures. The test calibration take this dilution into
account - it is not necessary to apply a dilution factor in the result calculation.
38
PROCEDURE - ORGANOPHOSPHONATE
1. - Fill the plastic test tube with diluted sample to the 20 ml mark.
2. - Add one Oxidising-OP tablet. Replace screw cap and shake tube until tablet dissolves. Allow the tube stand for five minutes.
3. - Add one OP-A tablet. Replace screw cap and shake tube until tablet dissolves. Allow the tube stand for two minutes
4. - Filter a portion of the solution into a round glass test tube filling to the 10 ml mark.
5. - Add one OP-B tablet, crush and mix to dissolve. Stand for five minutes to allow colour development.
6. - Select wavelength 620 nm on Photometer D-101. On Photoanalizer D-105 and D-100 Photometer select program nr. 42.
7. - Take Photometer reading. Make zero with the diluted sample, without tablets.
8. - Consult the calibration chart (Result A). On Photoanalyzer D105 and D-100 select program 42.
The test may be terminated at this stage if the original sample is know not to contain orthophosphate.
PROCEDURE- CORRECTION FACTOR

If it is suspected that the sample contains orthophosphate, carry out the following correction procedure:
1. - Fill a round glass test tube with diluted sample to the 10 ml mark.
2. - Add one OP-AX tablet. Crush and mix to dissolve.
3. - Add one OP-B tablet. Crush and mix to dissolve. Stand for five minutes to allow full colour development
4. - Select wavelength 620 nm on Photometer D-101. On Photoanalyzer D-105 and D-100 select program nr. 42.
5. - Take Photometer reading. Make zero with the diluted sample, without tablets.
6. - Consult the correction chart (Result B). On Photoanalyzer D-105 and D-100 select program 43.
Subtract this value (B) from the organophosphonate concentration previously obtained (A).
Corrected Organophosphonate (mg/l PO4) = Result A - Result B
RANGE: 0 -20 mg / l PO4 ORGANOPHOSPHONATE - RESULT A 620 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.06
0.1 0.2 0.4 0.5 0.7 0.8 1.0 1.1 1.3 1.4 1.6
0.2 1.7 1.9 2.0 2.2 2.3 2.5 2.6 2.8 3.0 3.1
0.3 3.3 3.4 3.6 3.7 3.9 4.0 4.2 4.3 4.5 4.6
0.4 4.8 4.9 5.1 5.3 5.4 5.6 5.7 5.9 6.0 6.2
0.5 6.3 6.5 6.7 6.8 7.0 7.1 7.3 7.4 7.6 7.7
0.6 7.9 8.1 8.4 8.8 9.1 9.5 9.8 10.1 10.5 10.8
0.7 11.2 11.5 11.8 12.2 12.5 12.9 13.2 13.6 13.9 14.3
0.8 14.6 15.0 15.3 15.7 16.0 16.5 16.9 17.3 17.8 18.2
0.9 18.7 19.1 19.6 20
RANGE: 0 -20 mg / l P4O PHOSPHATE CORRECTION - RESULT B 620 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.01 0.1 0.2 0.3 0.4 0.5
0.1 0.6 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5
0.2 1.6 1.7 1.8 1.9 2.0 2.1 2.2 2.3 2.4 2.5
0.3 2.6 2.8 2.9 3.0 3.1 3.2 3.3 3.4 3.5 3.6
0.4 3.7 3.8 3.9 4.0 4.1 4.3 4.5 4.6 4.8 5.0
0.5 5.2 5.4 5.5 5.7 5.9 6.1 6.2 6.4 6.6 6.8
0.6 6.9 7.1 7.3 7.5 7.6 7.8 8.0 8.2 8.5 8.8
0.7 9.0 9.3 9.5 9.8 10.1 10.3 10.6 10.9 11.1 11.4
0.8 11.6 11.9 12.1 12.3 12.6 12.9 13.1 13.4 13.6 13.9
0.9 14.1 14.4 14.6 14.9 15.1 15.4 15.6 15.9 16.1 16.5
1.0 16.9 17.3 17.6 18.0 18.4 18.8 19.1 19.5 20
INTERFERENCES
Chloride in excess of 350 mg / l will cause low results for organophosphonate. Sample containing chloride levels in excess of this value
should be further diluted prior to the start of the test.
The result of this test are expressed in terms of mg/l (ppm) active phosphate content. Commercially available products are normally
sold as aqueous formulations with a given active content. To utilise the test results, regard must be paid to the active content of the
product in use.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,426mg/L PO4
Traceability :The traceability of the method settles down with Dr. A. T. Palin
39
OZONE. Code 1.9445.00 (50t) - 1.9449.00 (250t) Photometer Method 520 nm
Test for Ozone in water. 0 - 2.0 mg / l
Ozone is used for disinfections of swimming pool, and many other water treatment system. Accurate measurement of Ozone residual is
essential for the control of these systems.
The Dinko DPD test is a simple means of measuring Ozone residuals up to a level of to 2.0mg / l.
METHOD
The DINKO test use the DPD method now internationally recognised as standard method of testing for disinfectant residuals. In the
DPD method the reagents are provided in tablet form for maximum convenience and simplicity of use. Ozone reacts with diethyl-p-
phenylene diamine (DPD) in buffered solution in the presence of potassium iodide to produce a pink coloration. The intensity of the
colour is proportional to the Ozone concentration and is measured using a DINKO Photometer.
For determination of Ozone in the presence of Chlorine or Bromine, a supplementary procedure using Glycine is used. The Glycine
destroys the Ozone in the sample and the colour produced in the DPD test corresponds to the Chlorine or Bromine only. The Ozone will
be the difference between the test readings with and without Glycine.

Tablet DPD nº 4 / Tablet DPD Glycine
Round cuvette 16 mm. Ø ( 4pcs ). Code 1.9365.00(cuvette used in the chart) Square cuvette 10 mm with cap.(100pcs). Code 1.9363.00
PROCEDURE
1. - Rinse test tube with sample leaving two to three drops of sample in the tube.
2. - Add one DPD Nº 4 tablet, crush tablet and then fill the test tube with sample to the 10 ml mark. Mix to dissolve.
4. - Take Photometer reading in usual manner. Make ZERO with water, without tablets.
Consult Ozone calibration chart (D-101) or select program Nnr.44 ( D-105 and D-100).
5. - The result represents the Ozone residual as milligrams per litre( Result A). If sample contents only Ozone the test may be
terminated at this stage.
6. - If sample contains Chlorine or Bromine, a correction should be made.
7. - Fill a test tube with sample to the 10 ml mark. Add one DPD Glycine tablet, crush and mix to dissolve.
8. -Take a second clean test tube and add two to three drops of solution from the first tube. Add one DPD Nr. 4 tablet, crush and then
add the remainder of solution to make up to the 10 ml mark. Mix to dissolve tablet.
9. -Take Photometer reading. Consult calibration chart(D-101) or select program nr.44 (D-100 and D-105).
Result B.
10.- Result B is the Ozone equivalent of the Chlorine or Bromine present. To obtain Ozone residual subtract this value
from the Ozone residual value obtained in the first procedure.
Ozone(mg/ l) = Result A - Result B

Range: 0 -2,0mg / L Ozone 520 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.02 0.04 0.07 0.09 0.11 0.14 0.16 0.18 0.21
0.1 0.23 0.25 0.28 0.30 0.32 0.34 0.37 0.39 0.42 0.44
0.2 0.47 0.49 0.52 0.54 0.57 0.59 0.62 0.64 0.67 0.69
0.3 0.72 0.74 0.77 0.79 0.82 0.85 0.88 0.91 0.94 0.98
0.4 1.00 1.04 1.07 1.10 1.13 1.16 1.19 1.22 1.25 1.28
0.5 1.30 1.33 1.36 1.39 1.42 1.45 1.48 1.50 1.53 1.56
0.6 1.59 1.62 1.64 1.67 1.70 1.72 1.75 1.78 1.81 1.83
0.7 1.86 1.89 1.91 1.94 1.97 2.00
Traceability: The traceability of the method of settles down with Palin, A.I. , J.Inst. Water Eng-1-21(6)537-547(1967)
pH (Phenol Red). Code 1.9417.00 (50t) - 1.9504.00 (250t) Photometer Method 520 nm
Test for pH in water and aqueous solutions. 6.8 - 8.4 pH
The Phenol Red indicator method provides a simple colorimetric means of pH determination for neutral and slightly alkaline waters over
the range 6,8-8,4 units.
METHOD
The test uses a tablet reagent containing the precise amount of phenol red indicator required for the test. Phenol red reacts in water at
different pH values a range of colours from yellow to red. The colour of the test solution is indicative of the pH value and is measured
using a DINKO Photometer.
Phenol red tablets contain a dechlorinating agent so that the test can be carried out in water containing normal levels of chlorine or other
desinfectant residuals.

Phenol Red Clear Tablet / Round cuvette 16 mm Ø with cap. (4pcs). Code 1.9365.00. (cuvette used in the chart)
40
PROCEDURE
1. - Fill test tube with sample to 10 ml mark and mix to dissolve.
2. - Add one Phenol Red tablet. Crush and mix to dissolve. Select the 520 nm filter.
3. - Take the Photometer reading. Make zero with sample without tablet.
4. - Consult calibration chart , Photometer D-101. Photometers D-100 and D-105 select program 45.
Notes
The formation of an intense purple coloration shows that the indicator has been affected by high chlorine or other desinfectant residuals.
In such cases the result should be disregarded.
Phenol red does not show any further colour change at pH values below 6,8 or above 8,4. Note therefore that when such values are
recorded this could indicate that the samples has a much lower or much higher pH value.
Ionic strength, temperature and other water factors may have an effect on pH readings. This test has been calibrated for conditions
most likely to be encountered in a typical swimming pool at 30ºC .
Range: 6,8- 8,4 pH Phenol red 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.1 6.80
0.2 6.85 6.90 6.95 7.00 7.00 7.00 7.00 7.00 7.05 7.05
0.3 7.05 7.10 7.10 7.10 7.10 7.15 7.15 7.15 7.15 7.20
0.4 7.20 7.20 7.25 7.25 7.30 7.30 7.35 7.35 7.40 7.40
0.5 7.40 7.45 7.45 7.50 7.50 7.50 7.55 7.55 7.60 7.60
0.6 7.60 7.60 7.60 7.65 7.65 7.65 7.65 7.70 7.70 7.70
0.7 7.70 7.75 7.75 7.75 7.80 7.80 7.80 7.80 7.85 7.85
0.8 7.85 7.85 7.90 7.90 7.90 7.95 7.95 7.95 7.95 8.00
0.9 8.00 8.00 8.05 8.10 8.10 8.15 8.15 8.20 8.20 8.25
1.0 8.30 8.35 8.35 8.40
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,026mg/L pH
Traceability:The traceability of the method of settles down with SM, “Standard Methods”
PHENOL. Code 1.9418.00 (50t) Photometer Method 520 nm

Test for Phenol and ortho/meta substituted phenols in natural, drinking
and industrial waste waters. 0- 5,0 mg /l as Phenol
Phenols and substituted phenols may occur in natural drinking and industrial waste waters. Phenols are not readily removed from water
by conventional water treatment process. These compounds arise typically from oil and chemical refining, livestock clips, the breakdown
of pesticides, human and animal wastes and from naturally occuring sources. Chlorination of such waters may produce odorous and
objectionable tasting chlorophenols. The DINKO Phenoltest methode provides a simple means of measuring the concentration of
phenol and phenolic compounds present in water over the range 0 - 5.0 mg/l. The concentration of phenol determined in the test is due
to unsubstituted and to ortho and meta substituted phenols. A proportion of “para” substituted phenols will give a positive response.
METHOD
In the Phenoltest method, phenol and phenolic compounds react with 4-aminoantipyrine in the presence of ferricyanide ions to form a
red colour. The reagents are provided in tablet form and the test is carried out simply by adding the appropriate tablets to a sample of
the water. A further tablet reagent is used to prevent interference due to metal ions. The intensity of the red colour produced in the test
is proportional to the concentration of phenolic compounds present in the sample and is measured using a DINKO Photometer.

Phenoltest nr.1 Tablet / Phenoltest nr. 2 Tablet / Phenoltest PR Tablet
Round cuvette 16mm Ø with cap.(4pcs). Code 1.9365.00. (cuvette used in the chart).
PROCEDURE
1. - Fill round test tube to 10 ml mark with sample.
2. - In the case of samples known to contain Copper, Zinc, Iron or Manganese ions, add one Phenoltest PR tablet. Crush and mix to
dissolve.
3. - Add one Phenoltest nr. 1 tablet, crush and mix to dissolve.
4. - Add one Phenoltest nr. 2 tablet, crush and mix to dissolve.
6. - Select wavelength 520 nm on the photometer.
7. - Take Photometer reading (see photometer instructions). Make zero with the sample without tablets.
8. - Consult the Phenol calibration chart (D-101). Select program nr. 46 (D-105 and D-100).
INTERFERENCES
1- Use of the Phenoltest PR tablet will prevent interference from metal ions up to a concentration of 350 mg/l. The test is unaffected by
free chlorine in the sample up to 10 mg/l
2- Low results may be obtained in sample containing more than 150 mg/l alkalinity (as CaCO3), 10 mg/l sulphite or 2 mg/l
sulphide. Certain organic keto-enol compounds may cause high results. In case of known or suspected interferences, then the
sample should be-treated in accordance with standard analytical procedures.
41
RANGE: 0- 5,0 mg / L Phenol 520 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.05 0.12 0.19 0.26 0.33 0.40 0.47 0.53
0.1 0.60 0.67 0.74 0.81 0.88 0.95 1.02 1.09 1.16 1.22
0.2 1.29 1.36 1.43 1.50 1.58 1.64 1.72 1.79 1.86 1.93
0.3 2.01 2.08 2.15 2.22 2.30 2.37 2.44 2.51 2.58 2.65
0.4 2.72 2.79 2.85 2.92 2.99 3.06 3.13 3.20 3.26 3.33
0.5 3.40 3.47 3.54 3.60 3.67 3.74 3.81 3.88 3.95 4.01
0.6 4.09 4.16 4.24 4.31 4.39 4.46 4.54 4.61 4.69 4.76
0.7 4.85 4.92 5.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,099 mg/L as Phenol
Traceability: The traceability of method settles down with SM, “Standard Methods” 6th Ed. V2. 2,51,2464(1975) and USEPA 420.1
PHMB. Code 9420 (50t) Photometer Method 620 - 630 nm

Test for PHMB-based sanitizers in swimming pool water. 0 -100 mg / l
Polyhexamethylbiguanide (PHMB) is an organic biocide used for water desinfection. PHMB-based sanitizers are widely used for
treatment of swimming pool water. These sanitizers are typically sold under branded names, for example Baquacil (Zeneca), Softswim
(Biolab), Revosil (Mareva) and Nicosil (Nico Norge).
The test provides a simple means of measuring PHMB-base sanitiser levels in swimming pool waters over the range 0-100 mg/l. The
test is calibrated in terms of commercially available sanitizers products which normally contain 20% active biocide.
METHOD
In the test the PHMB reacts with a sulphonephtalein indicator under midly acid conditions to form a intense blue complex. The indicator
itself is yellow in colour. Thus at different PHMB levels a distinctive range of colours from yellow, through green, to blue are produced.
The reagents are combined in the form of a single of water. The intensity of colour produced is proportional to the PHMB concentration
and is measured using a DINKO Photometer.

PHMB Tablet / Round cuvette 16 mm Ø with cap. (4pcs ). Code 1.9365.00. (cuvette used in the chart)
Square cuvette 10 mm with cap. (100pcs). Code: 1.9363.00 / DINKO D-101 Photometer use the calibration chart, filter 620 nm.
PROCEDURE
1. - Fill test tube with sample to the 10 ml mark. Crush and mix to dissolve.
2. - Add one PHMB tablet. Crush and mix to dissolve.
3. - Select filter 620 nm (D-101 Photometer). D-105 Photoanalyzer and D-100 Photometer select program nr. 47
4. - Take the Photometer reading. Make zero with sample without tablet.
5. - Consult calibration chart , Photometer D-101. Photometers D-100 and D-105 select program nr.47.
Range: 0 -100 mg / L PHMB 620 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.00 2.79 5.56 8.33 10.8 12.7 14.7 16.6 18.5
0.1 20.5 22.4 24.4 26.3 28.3 30.2 32.1 34.1 36.0 38.0
0.2 39.9 41.9 43.8 45.7 47.7 49.6 52.0 54.4 56.9 59.4
0.3 61.9 64.3 66.8 69.3 73.2 77.8 82.4 87.0 91.6 96.3
0.4 100
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 3,403mg/L PHMB
Traceability: The traceability of the method of settles down with Dr. A. T. Palin
PHOSPHATE. Code 1.9432.00 (50t) - 1.9482.00 (200t) Photometer Method 620- 630 nm
Test for low levels of phosphate in natural and drinking water 0 - 4,0 mg / l PO4
Phosphates are extensively used in detergent formulations and washing powders. Also find application in the food processing industry
and in industrial water treatment processes. Agricultural fertilisers normally contain phosphate minerals and phosphates also arise from
the breakdown of plant materials and in animal wastes.
Whilst phosphates are not generally considered harmful for human consumption, they do exhibit a complex effect on the natural
environment. Phosphates are associated with eutrophication of water and with rapid unwanted plant growth in rivers and lakes.
The DINKO test provides a simple method of measuring phosphate levels over the range 0- 4 mg /l of PO4 . For drinking water the ECC
has set a guide level of 0,5mg/l PO4 (0,4 mg/l P2 O5) and a maximum admissible concentration of 6,7 mg/l PO4 (5 mg/l P2 O5).
METHOD
The phosphate reacts under acid conditions with ammonium molybdate to form phospho-molybdic acid. This compound is reduced by
ascorbic acid to form the intensely coloured “ molybdenum blue” complex. A catalyst is incorporated to ensure complet and rapid colour
development, and a inhibitor is used to prevent interference from silica. The reagents are provided in the form of two tablets for
maximum convenience. The test is simply carried out by adding one of each tablet to a sample of the water.
The intensity of the colour produced in the test is proportional to the phosphate concentration, and is measured using a DINKO
Photometer.
42
REACTIVOS Y EQUIPO
Phosphate nº 1 LR tablet / Phosphate nº 2 LR tablet/ Round cuvette 16 mm Ø with cap. (4pcs ). Code: 1.9365.00. (cuvette used in the
chart) / Square cuvette 10 mm with cap. Pk.(100). Code 1.9363.00
DINKO D-101 Photometer use the calibration chart. Filter 620 nm
DINKO D-105 Photoanalyzer and D-100 select program nr.48
PROCEDURE
2. - Add one Phosphate nº 1 LR tablet, crush and mix to dissolve.
3. - Add one Phosphate nº 2 LR tablet, crush and mix to dissolve.
4. - Stand for ten minutes to allow full colour development.
5. - Select wavelength 620 nm (D-101). Select program nr. 48 on the Photometers D-105 and D-100.
6. - Take Photometer reading (see Photometer Instructions). Make zero with the sample without tablets.
7. - Consult Phosphate calibration chart ( D-101) or select program nr.48 (D-105 and D-100)
RANGE: 0- 4,0 mg / L PHOSPHATE mg/ L PO4 620 – 630 nm

ABS. 0 1 2 3 4 5 6 7 8 9
0.0 0 0.04 0.08 0.13 0.17 0.22 0.26 0.31
0.1 0.35 0.39 0.44 0.48 0.53 0.58 0.63 0.68 0.73 0.77
0.2 0.82 0.87 0.92 0.97 1.01 1.06 1.11 1.16 1.21 1.26
0.3 1.31 1.36 1.40 1.45 1.50 1.55 1.60 1.65 1.70 1.75
0.4 1.80 1.84 1.89 1.94 1.99 2.05 2.11 2.17 2.24 2.30
0.5 2.36 2.42 2.48 2.55 2.61 2.67 2.73 2.80 2.86 2.92
0.6 2.98 3.05 3.13 3.21 3.29 3.37 3.45 3.52 3.60 3.68
0.7 3.76 3.84 3.91 4.00
Phosphate concentrations can be expressed in a number of different ways.The following factors may be used for the conversion of
readings:
To convert from PO4 to P2 O5 multiply by 0,75 / To convert from PO4 to P multiply by 0,33
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,085mg/L as PO4
Traceability: The traceability of method settles down with “UEPA 365.2” and “SM4500-P-E”
PHOSPHATE. Code 1.9426.00 (50t) - 1.9462.00 (250t) Photometer Method 490 nm

Test for high levels of phosphate in boiler water 0-100 mg / l PO4
Phosphates are extensively used for treatment water in boilers and steam raising plant. Phosphates are added to control the deposition
of sediment within the boiler and is essential to know the concentration. The DINKO Phosphate test provides a simple method of
measuring phosphate levels in boiler waters over the range 0 - 100 mg /l de PO4.
METHOD
The DINKO Phophate test is base on the vanadomolybdate method. All the reagents required are provided in form of a test tablet. The
test is carried out by adding a single tablet to a sample of the boiler water. In the test phosphates react with ammonium molybdate, in
presence of ammonium vanadate, to form the yellow phosphovanadomolybdate. The intensity of the colour produced in the test is
proportional to the phosphate concentration and is measured using a DINKO Photometer.
SAMPLE COLLECTION
Samples drawn from boiler sampling points may be hot and contain particulate matter. Prior to analysis samples should be cooled to
below 25ºC and filtered.

Phosphate HR Tablets
DINKO D -101 Photometer use the calibration chart. Photoanalyzer D -105 and D-100 select program nr.49.
Round cuvewtte 16 mm. Ø with cap (4pcs). Code 1.9365.00 (cuvette used in the chart) / Square cuvette 10 mm. (100pcs). Code
1.9363.00
PROCEDURE
2. - Add one Phosphate HR tablet, crush and mix to dissolve.
3. - Stand for teen minutes to allow colour development. Select wavelength 490 nm on Photometer
4. - Take photometer reading (see photometer instructions). Make zero with the sample without tablets
5. - Consult Phosphate calibration chart (D-101) or select program nr.49 (D-100 and D-105).
RANGE: 0-100 mg/L mg//L PHOSPHATE PO4 490 nm

ABS. 0 5 ABS. 0 5 ABS. 0 5
0.07 0.0 0.4 0.15 30 33 0.23 71 73
0.08 2 4 0.16 35 38 0.24 76 78
0.09 6 8 0.17 40 43 0.25 81 84
0.10 9 11 0.18 45 48 0.26 87 91
0.11 13 15 0.19 50 53 0.27 94 97
0.12 17 19 0.20 55 58 0.28 100
0.13 21 23 0.21 61 63
0.14 25 28 0.22 66 68
43
The following factors may be used for conversion of readings:
To convert from PO4 to P2O5 multiply by 0,75 / To convert from PO4 to P multiply by 0,33
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 7.088mg/L PO4
Traceability:The traceability of method settles down with “ SM, “ Standard Methods”
PHOSPHORUS, TOTAL. Code 1.9007.00 (25t) Photometer Method 620- 630 nm

Test phosphorus compounds in natural and waste water 0 - 12,0mg / l P
Total Phosphorus is composed of orthophosphates, polyphosphates and organic phosphorus compounds. Ortho and polyphosphates
are extensively used in detergents formulations and washing powders. Phosphates and phosphorus compounds also find widespread
application in the food processing industry, industrial water treatment process, agricultural fertilisers and are found in animal wastes or
from certain manufacturing processes.
The monitoring of Total Phosphorus is therefore of great importance .
The DINKO test provides a simple method of measuring total Phosphorus compounds over the range 0 -12,0mg /l P
METHOD
The DINKO tubetest is a simple two stage procedure. The sample is first digested with acid persulphate to break down polyphosphates
and organic phosphorus compounds and convert them to orthophosphate. Then all phosphate present in the sample is determined by
reaction with ammonium molybdate and ascorbic acid to form a intensely coloured “molybdenum blue” complex “. A catalyst is
incorporated to ensure complete and rapid colour development, and an inhibitor is used to prevent interference from silica.
The intensity of colour produced is proportional to the total phosphorus concentration, and is measured using a DINKO Photometer
REAGENTS AND EQUIPMENT –Digestion Stage

Reagent Tubes, 25u / Digest Ox Tablets / Heater block DINKO model D-64 or D-65 / Pipettor, 2ml
REAGENTS AND EQUIPMENT- Colour Development Stage

PhosNeut Solution Phos Nr. 1 Tablets / Phos nr. 2 Tablets / Photometer D-101 use the chart, filter 620 nm.
Photoanalyzer DINKO D-105 and Photometer D-100 select program nr. 59.
PROCEDURE – Disgestion Stage

1- Turn on the Heater block, set to 100-105º C and allow to heat up the temperature.
2- Remove the cap of the tubetest and add 2 ml of sample using the pipettor.
3- Add two Digest Ox tablets, crush and mix to dissolve.
4- Replace te cap tightly and invert tube gently to mix. Label the tube and place in the Heater block. Ensure the safety bell is in position.
Digest the tube for one hour(minimum 45 minutes) then turn of the heater.
5- Carefully remove the tube, transfer to a test tube rack and allow to cool to room temperature.
PROCEDURE- Colour Development Stage

1- Carefully remove the cap from the cooled tube and add 2,0ml of Phosneut solution using the pipettor.
2- Add one Phos nr. 1 tablet, crush and mix to dissolve. Ensure all particles of the tablet have dissolved.
3- Add one Phos nr. 2 tablet, crush and mix to dissolve. Cap tube and gently invert several times to mix.
4- Stand tube for 10 minutes to allow colour development.
5- Select filter 620 nm on D-101 Photometer. For D-105 Photoanalyzer and Photometer D-100 select program nr. 59
6- Take the photometer reading. Use an unused Tubetest Reagent to set the zero on the Photometers.
Also a tube containing deionised water only may be used.
7- Consult the Phosphorus table. Select program nr. 59 in the model D-105 and D-100.
RANGE: 0 -12,0 mg / L PHOSPHORUS,TOTAL P 620 - 630 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.04 0.12 0.20 0.28 0.36 0.43 0.51 0.59 0.67
0.1 0.74 0.82 0.89 0.97 1.04 1.11 1.19 1.26 1.34 1.41
0.2 1.49 1.57 1.64 1.71 1.79 1.86 1.93 2.01 2.08 2.16
0.3 2.23 2.31 2.38 2.46 2.53 2.61 2.68 2.76 2.83 2.91
0.4 2.98 3.06 3.13 3.21 3.28 3.35 3.43 3.50 3.58 3.65
0.5 3.73 3.80 3.88 3.97 4.06 4.15 4.24 4.34 4.43 4.52
0.6 4.62 4.71 4.80 4.90 4.99 5.08 5.17 5.27 5.36 5.45
0.7 5.55 5.64 5.73 5.58 5.92 6.01 6.11 6.20 6.29 6.38
0.8 6.48 6.57 6.66 6.80 6.96 7.12 7.28 7.48 7.60 7.76
0.9 7.92 8.08 8.24 8.40 8.56 8.72 8.88 9.04 9.20 9.36
1.0 9.52 9.68 9.84 10.0 10.2 10.3 10.5 10.6 10.8 11.0
1.1 11.1 11.3 11.4 11.6 11.8 12.0
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,014 mg/L P
Traceability:The traceability of Phosphorus Total method settles down with USEPA 365.2
44
POTASSIUM. Code 1.9456.00 (50t) - 1.9494.00 (250t) Photometer Method 520 nm
Test for Potassium in natural and treated water. 0 -12,0 mg / l
Potassium is an abundant natural element. However in fresh water potassium levels are normally low. The guide level prescribed for
drinking water supplies under the EEC Regulations is 10 mg/l .The DINKO Potassium test provides a simple means of testing potassium
levels in water over the range 0-12,0 mg / l.
METHOD
The DINKO Potassium test is based on a single tablet containing sodium tetraphenylboron. Potassium salts react with sodium
tetraphenylboron to form an insoluble white complex. At the potassium levels encountered in th test, this is observed as a turbidity in the
test sample. The degree of turbidity is proportional to the Potassium concentration and is measured using a DINKO Photometer.

Potassium K Tablet/Round cuvette 16 mm Ø with cap.(4pcs). Code 1.9365.00(cuvette used in the chart)/Square cuvette 10 mm with
cap. (100pcs). Code 1.9363.0 /D-101 Photometer use the calibration chart /D-105 Photoanalyzer and D-100 Photometer select program
nr. 20
PROCEDURE
2. - Add one Potassium K tablet, crush and mix to dissolve. A cloudy solution indicates the presence of Potassium.
3. - Select wavelength 520 nm on the Photometer.
4. -Take Photometer reading (see Photometer Instructions). Make zero with the sample without tablet.
5. - Consult Potassium calibration chart (D-101). Select program nr. 20 (D-100 and D-105).
Range: 0-12,0 mg/L K PPM POTASSIUM 520 nm

ABS 0 2 6 8 ABS 0 2 6 8
0.0 0.0 0.4 1.0 1.4 1.1 7.1 7.2 7.4 7.5
0.1 1.8 2.0 2.1 2.2 1.2 7.7 7.8 8.0 8.1
0.2 2.3 2.4 2.5 2.6 1.3 8.2 8.4 8.6 8.7
0.3 2.7 2.8 2.9 3.0 1.4 8.8 8.9 9.2 9.3
0.4 3.1 3.2 3.4 3.5 1.5 9.4 9.5 9.8 9.9
0.5 3.6 3.7 4.0 1.6 1.6 10.0 10.1 10.3 10.4
0.6 4.2 4.3 4.5 4.7 1.7 10.4 10.6 10.8 10.9
0.7 4.8 4.9 5.1 5.2 1.8 11.0 11.1 11.4 11.5
0.8 5.3 5.5 5.7 5.8 1.9 11.6 11.7 11.9 12.0
0.9 5.9 6.0 6.3 6.4 2.0
1.0 6.5 6.6 6.8 7.0
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,089 mg/L K
Traceability: The traceability of the method of settles down with SM, “Standards Methods”
SILICA. Code 1.9457.00 (50t) - 1.9495.00 (200t) Photometer Method 620 - 630nm
Test for Silica in natural treated and industrial water 0- 4,0mg / l SiO2
Silicon, in the form of silica, is one of the earth’s most abundant elements. Silicon is found widely in natural waters as colloidal silica or
soluble silicates. Silica and silicates do not normally cause any problems in water intended for domestic consumption. However their
presence is undesirable in water used in a variety of industrial application. This is because of the tendency of such water to form a hard
scale on equipment. Silica and silicate containing waters are particularly troublesome in steam generating plant such as high pressure
boilers since silica scale can build up on turbine blades.
The test provides a simple means to measuring silica and silicate levels in natural, treated and industrial waters over the range 0-4 mg/l
SiO2
METHOD
Ammonium molybdate reacts with silica under acid conditions to produce molyb-dosilicic acid. In the presence of a reducing agent, this
compound is reduced to form an intense blue complex. Phosphate reacts in a similar manner. Interference by phosphate is prevented
by introducing a reagent which destroy any molybd-dophosphoric acid which may form. The reagents are provided in tablet form and
the test is carried out by adding tablets to a sample of water. The intensity of colour produced in the test is proportional to the silica
concentration and is measured using a DINKO Photometer.
Silica nr.1 Tablet / Silica nr. 2 Tablet / Silica PR Tablet / Round cuvette 16 mm. Ø with cap (4pcs). Code 1.9365.00/ DINKO D-101
Photometer, use calibration chart, filter 620 nm./ DINKO D-105 and D-100 Photometers use program nr. 21
PROCEDURE
1. - Fill tube test with sample to the 10ml mark.
2. - Add one Silica nr. 1 tablet, crush and mix to dissolve. Stand for 5 minutes to allow the silica to react.
3. - Add one Silica PR tablet, crush and mix to dissolve. This stage may be omitted if sample is free of phosphate.
4. - Add one Silica nr. 2 tablet, crush and mix to dissolve. Stand for 5 minutes to allow colour development.
5. - Select filter 620 nm. for D-101. For D-100 Photometer and D-105 Photoanalyzer select program nr. 21
6. - Take photometer reading. Make zero with sample without tablets.
7. - Consult Silica calibration chart with D-101 Photometer. With D-105 and D-100 Photometers select program nº 21.
45
RANGE: 0- 4,0 mg / L Sílica SiO2 620 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 0.04 0.09
0.1 0.14 0.19 0.24 0.29 0.34 0.39 0.44 0.49 0.54 0,. 0
0.2 0.67 0.73 0.79 0.85 0.91 0.98 1.03 1.09 1.14 1.19
0.3 1.25 1.30 1.35 1.41 1.46 1.52 1.57 1.62 1.68 1.73
0.4 1.78 1.84 1.89 1.95 2.00 2.06 2.12 2.18 2.24 2.30
0.5 2.36 2.42 2.48 2.55 2.61 2.67 2.73 2.79 2.85 2.91
0.6 2.97 3.03 3.08 3.13 3.18 3.23 3.28 3.33 3.38 3.44
0.7 3.49 3.54 3.59 3.64 3.70 3.74 3.79 3.85 3.90 3.95
0.8 4.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,071 mg/L SiO2
Traceability: The traceability of the method of settles down with SM, “Standards Methods”
SILICA. Code 1.9421.00 (50t) - 1.9453.(200t) Photometer Method 420- 450 nm

Test for Silica in natural, treated and industrial water. 0 - 150mg / l SiO2
Silicon, in the form of silica, is one of the earth´s most abundant elements and do not cause problems in water intended for domestic
consumption. Their presence is undesirable because of the tendency to form a hard scale on equipment.
The DINKO test provides a simple means of measuring silica and silicates levels over the range 0 -150 mg / l SiO2
METHOD
Sodium molybdate reacts with silica under acid conditions to produce molybdosilicic acid. Phosphate reacts in similar manner.
Interference by phosphate is prevented by introducing a reagent which destroy any molybdophosphoric acid which may form. The
regents are provided in tablet form to be added in a sample of water. The intensity of colour produced in the test is proportional to the
silica concentration and is measured using a DINKO Photometer.

Silica HR Nr. 1 Tablet / Silica HR nr. 2 Tablet / Silica PR Tablet / Round cuvette 16 mm. Ø with cap (4pcs). Code 1.9365.00
DINKO D-101 Photometer, use calibration chart, filter 420 nm.
DINKO D-105 and D-100 Photometers, select program nr. 50
PROCEDURE
1. - Fill the test tube with sample to the 10 ml mark.
2. - Add one Silica HR nr. 1tablet, crush and mix to dissolve.
3. - Add one Silica HR nr. 2 tablet, crush and mix to dissolve. Stand for 10 minutes to allow full colour development.
4. - Add one Silica PR tablet, crush an mix to dissolve. Stand for two minutes. This stage may be omitted if the sample is free of
phosphate and chlorine.
5. - Select wavelength 420 nm. on D-101 and on D-100 and D-105 Photoanalyzer select program nr. 50
6. - Take Photometer reading. Make zero with sample without tablets.
7. - Consult Silica calibration chart with D-101Photometer. Select program nr. 50 with D-105 and D-100 Photometers.
RANGE: 0-150 mg / L Silica SiO2 420 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 1 2 4 5 6 8
0.1 9 10 12 13 15 16 17 19 20 21
0.2 23 24 26 27 28 30 32 34 35 37
0.3 39 41 43 45 47 49 51 53 55 57
0.4 59 61 64 66 69 71 74 76 78 81
0.5 83 86 88 91 94 98 101 104 107 110
0.6 113 116 119 123 128 132 137 141 145 150
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 4.253mg/L SiO2
Traceability: The traceability of the method of settles down with SM, “Standards Methods
SULPHATE. Code 1.9458.00 (50t) - 1.9496.00 (200t) Photometer Method 520 nm

Test for sulphate in natural and treated waters. 0 - 200 mg / l
Sulphates are introduced into treated waters by the use of such chemicals as aluminium sulphate, Sodium bisulphate and Sulphuric
acid. The presence of high levels of sulphate can be undesirables. In industrial waters containing sulphate localised corrosion of Iron,
steel and Aluminium in plant and pipe work can occur through the action of sulphate-reducing bacteria. These bacteria, which generate
sulphides, cause a characteristic pitting of the metal surface. High sulphate levels can also cause damage to concrete and cement
based materials through the formation of Calcium sulphoaluminate. This cause expansion and crumbling of cement. It can affect
concrete structures and pipes in water distribution systems carrying sulphate-bearing ground waters; and can attack grouting in tiled
swimming pools using Sodium bisulphate for pH adjustement.Test range is 0-200 mg/l SO4 Higher levels may be determined by diluting
sample.
46
METHOD
The DINKO Sulphate test is based on a single tablet reagent containing Barium chloride in a slightly acidic formulation. Barium salts
react with sulphates to form insoluble Barium sulphate. At the sulphate levels encountered in the test, this is observed as turbidity in the
test sample. The degree of turbidity is proportional to the sulphate concentration and is measured using a DINKO Photometer.

Sulphate Turb Tablets / Square cuvette 10 mm. (100pcs). Code 1.9363.00
Round cuvette 16 mm. Ø with cap (4pcs). Code 1.9365.00 (cuvette used in the chart).
D-101 Photometer use calibration chart / D-105 Photoanalyzer and D-100 select program nr. 22.
PROCEDURE
2. - Add one Sulphate Turb tablet, crush and mix to dissolve. Select wavelength 520 nm on the photometer.
3. - Take Photometer reading(see photometer instructions). Make zero with sample without tablet.
4. - Consult Sulphate calibration chart(D-101). Select program nr. 22 (D-105 and D-100)
Range: 0 - 200 mg / l SULPHATE SO4 520 nm

ABS 0 2 6 8 ABS 0 2 6 8
0.0 0 2 7 10 1.1 105 106 110 111
0.1 12 15 20 22 1.2 113 115 118 120
0.2 24 26 31 33 1.3 122 123 127 128
0.3 35 38 42 43 1.4 130 132 135 137
0.4 45 47 50 52 1.5 139 145 145 147
0.5 54 55 59 60 1.6 149 151 155 157
0.6 62 64 67 69 1.7 159 162 166 169
0.7 71 72 76 77 1.8 170 172 176 178
0.8 79 81 84 86 1.9 181 185 192 196
0.9 88 89 93 94 2.0 200
1.0 96 98 101 103
CAUTION
Sulphate tablets each contain 20 mg Barium Chloride. These tablets are harmful if ingested. Avoid handling tablets whenever possible
and wash hands after use.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 1,063 mg/L SO4
Traceability: The traceability of the method of settles down with USEPA 375.4
SUPHIDE Código 1.9460.00 (50t) Photometric Method 620- 630 nm

Test for sulphide in natural and waste water 0- 0,5 mg/ l
Natural waters containing dissolved Hydrogen sulphide and other sulphides are found in certain parts of the world, in areas having hot
springs. Sulphides are constituents of many industrial wastes such as those from tanneries, gas plants and chemicals works. Sulphides
can be toxic to fish and aquatic life and their presence in water supplies gives rise to undesirable tastes and odours.
The DINKO Sulphide test provides a simple method of measuring total available sulphide over the range 0 to 0,5mg/l and is particularly
applicable to natural and drinking waters. Higher levels , such as those found in effluents and waste waters, can be determinate by
diluting the sample.
METHOD
The method is based on a reagent containing Diethyl-p-phenylenediamine (DPD) and Potassium dichromate. Sulphide reacts with DPD
to produce a blue complex. In the absence of sulphides the reagent is pink colour. Chlorine, and other oxidising agents, do not interfere
with the test. The reagents are provided in two tablets. The colour produced is measured using a DINKO Photometer.

Nr. 1Tablets / Nr. 2 Tablets / Round cuvettes 16 mm Ø with cap (4pcs). Code:1.9365.00 (cuvette used in the chart)
DINKO D-101 Photometer use calibration chart. Filter 620 nm
DINKO D-105 Photoanalyzer and D-100 Photometer select program nr. 23
SAMPLE COLLECTION
Collect the sample carefully with a minimum of agitation and test immediately after collection.
PROCEDURE
1.- Fill the test tube with sample to 10 ml mark.
2.- Add one Nr. 1 tablet and one Nr. 2 tablet, crush and mix gently to avoid loss of sulphide.
3.- Stand 10 minutes to allow full colour development.
4.- Select wavelength 620 or 630nm on the Photometer.
5.- Take photometer reading (see photometer instructions). Make blank without tablets.
6.- Consult the Sulphide calibration chart (D-101). Select program nr. 23 (D-105 and D-100).
47
Range: 0-0,5 mg / l Sulphide 620 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.1 0.00 0.01 0.02 003
0.2 0.04 0.05 0.06 0.07 0.08 0.09 0.10 0.11 0.12 0.13
0.3 0.14 0.15 0.16 0.17 0.18 0.19 0.20 0.21 0.22 0.23
0.4 0.24 0.25 0.26 0.27 0.28 0.29 0.30 0.31 0.33 0.34
0.5 0.35 0.35 0.36 0.36 0.37 0.38 0.38 0.39 0.39 0.40
0.6 0.41 0.41 0.42 0.43 0.43 0.44 0.45 0.47 0.48 0.49
0.7 0.50 To convert from mg/l S to mg/l H2S multiply results by 1.06
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is ± 0,014 mg/L S -
Traceability:The traceability of the method of settles down with USEPA 376.2 and SM 4500-S2
SULPHITE. Code 1.9459.00 (50t) - 1.9497.00 (250t) Photometric Method 580- 577 nm
Test for sulphite in boiler water 0- 500 mg/l Na2SO3
Oxygen is major cause of corrosion in boilers and steam raising plant. Sodium sulphite and catalysed sulphite formulations are
extensively used as oxygen scavengers in boiler water treatment. The test covers the range 0 a 500 mg / l.
METHOD
The method is based on a colorimetric procedure involving the reduction of an indicator. Sulphites react with the indicator under
buffered conditions to destroy the original purple coloration. With increasing sulphite concentrations a range of colours from purple to
colourless is produced. This method does not respond to other reducing species as do traditional iodometric methods. The degree of
colour loss in the test is proportional to the sulphite concentration and is measured using a DINKO Photometer.

Nº 1 Tablets/ Nº 2 Tablets / Round cuvette 16 mm. Ø with cap (4pcs). Code 1.9365.00 (cuvette used in the chart)
Square cuvette 10 mm. with cap.(100pcs). Code1.9363.00 / DINKO D-101 Photometer use calibration chart, filter 580 nm.
DINKO D-105 Photoanalyzer and D-100 Photometer select program nr. 24
PROCEDURE
1.- Filter sample if necessary to obtain a clear solution. Fill the test tube with sample to 10 ml mark.
2.- Add one Nr. 1 tablet, crush and mix to dissolve.
3.- Add one Nr. 2 tablet, crush and mix to dissolve. Cap tube immediately. Stand two minutes.
4.- Select filter 580 on the Photometer D-101. Select program nr. 24 with photometers D-100 and D-105
5.- Take photometer reading (see photometer instructions). Make zero with sample without tablets.
6.- Consult the Sulphite calibration chart(D-101). Select program 24 (D-100 and D-105).
Equipment should be washed immediately after use, with a detergent if necessary, to prevent staining.
RANGE: 0-500 mg / L Sulphite (Na2SO3) 580 nm

ABS. 0 1 2 3 4 5 6 7 8 9
0.0
0.1 500 495 440 385 330 285 260 235 210 190
0.2 170 155 140 125 110 98 93 87 82 77
0.3 72 67 62 57 52 49 47 45 44 42
0.4 40 39 37 35 34 32 30 28 27 25
0.5 23 22 20 18 16 15 13 11 9 8
0.6 6 4 3 1
To convert from mg/l Na2SO3 to mg/l SO3 multiply by 0,63.
INTERFERENCES
1.- This test is not affected by presence of other reducing species such as nitrite (up to 200 mg/l), ferrous iron (up to 20 mg/l) and
sulphide ( up to 10 mg/l); or by the presence of polyacrylates.
2.- Chlorine up to 250 mg/l does not cause interference, However, since sulphite and Chlorine do not normally co-exist, the test will not
usually be carried out in the presence of Chlorine.
3.- The test gives low results if used in presence of Tannic acid or Tannin treated waters.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is: to 50 mg/L ± 5,148 mg/L Na2SO3 , from 50 to
100 mg/L ± 7,280, from 100 to 300 mg/L ± 28,36 and from 300 to 500 mg/L ± 63,61mg/L Na2SO3
Traceability: The traceability of the method settles down with “SM”
48
SURFACTANTS, ANIONICS. Code 1.9371.00(50t) Photometric Method 630-620 nm
Test for anionic surfactants in water 0,00 -4,0 mg / l
The surfactants are present in all the detergents, industrial or domestic. The surfactants are used abundantly in the daily life. The
residual waters contain them, being able to contaminate drinking waters. The surfactants remains in pipes and deposits cleaned with
detergents and not well clarified.
METHOD
The Methylene Blue is soluble in Trichlormethane. In presence of anionic surfactants the Methylene Blue reacts forming compounds
that can recovery with Trichlormethane, that is colored of blue proportionally to the quantity of surfactants present in the aqueous
sample. The method determines all the surfactants that react with Methylene Blue or MBAS (Methylene Blue Active Substances),
mainly anionic surfactants of the type sulphates and sulphonates. As the molecular weight of these substances are very different, the
result is calculated in mg/l of Sodium Dodecylsulphate.

Extractor solvent. Code 1.9371.01 / Surfactants reagent nr. 1 / Surfactants Reagent nr. 2 / Tube reagent / Empty tube zero / Pippetor
Round cuvette 16mm Ø with cap ( 4pcs ). Code1.9365.00. (cuvette used in the test).
DINKO D-101 Photometer use the calibration chart, 620 nm filter.
DINKO D-105 Photoanalyzer and D-100 Photometers select program nr. 57
PROCEDURE
1.- Fill tube reagent with 5 ml of Extractor Solvent using the pippetor. See notes.
2.- Add 5 ml of sample. No mix.
3.- Add 3 drops of Surfactants reagent nr.1. No mix.
4.- Add 3 drops of Surfactants reagent nr. 2
5.- Replace the cap tightly and invert tube gently four times to mix contents. Stand 1 minute and invert against tube four times to mix.
The sample should be clear. Due to a inadequate room temperature can appear turbidity that it will falsify the reading. In these
cases to simply heat with the hand the tube until the sample is completely clear. Take photometer reading immediately
6.- Select filter 620 nm. On D-101 Photometer. Make zero using the Empty tube zero with 5 ml of Extractor Solvent only.
7.- Select program nr. 57 with D-105 an D-100 Photometers. Use chart with D-101 Photometer
Range: 0,0-4,0 mg / L Anionic surfactans 620 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.0 0.0 0.05 0.09 0.14 0.19 0.23 0.28 0.33 0.37
0.1 0.42 0.47 0.51 0.56 0.61 0.65 0.70 0.74 0.79 0.84
0.2 0.88 0.93 0.98 1.03 1.07 1.12 1.17 1.21 1.26 1.31
0.3 1.35 1.40 1.45 1.49 1.54 1.59 1.63 1.68 1.73 1.77
0.4 1.82 1.87 1.91 1.96 2.01 2.05 2.10 2.15 2.19 2.24
0.5 2.29 2.34 2.38 2.43 2.49 2.54 2.60 2.65 2.71 2.76
0.6 2.82 2.87 2.93 2.98 3.04 3.09 3.15 3.20 3.26 3.31
0.7 3.37 3.43 3.49 3.54 3.60 3.66 3.71 3.77 3.83 3.89
0.8 3.95 4.00
The following problems can happen when will make the photometer readings:
1 - The colored side of the tube (Extractor solvent) it is cloudy.
2 - Watery drops exist in the tube wall corresponding to Extractor solvent side.
Anyone of these two problems prevents to make correct readings.
It is necessary to use very clean tubes exempt of residual detergents coming from not well clarified laundries that they generate error
and can favour the appearance of bubbles.
To mix by invert tube and to heat with the hand to avoid the turbidity
If the drops of the inside wall of the tube don't disappear, to incline the tube and to carry out some rotations to detach the drops.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is ± 0,071mg/L Anionic surfactants
Traceability: The traceability of the method of settles down with SM “Standard Methods” 5540C
TURBIDITY . Code 1.9444.00( 50t) Photometer Method 520 nm.

Test for turbidity in natural and treated waters 5 - 400 Turbidity Units
The turbidity is a important measure to know the water quality. It is caused by the scattering of light by suspended matter. A knowledge
of turbidity is a estimation of the concentration of suspended substances
METHOD
The turbidity is determined using a DINKO Photometer. In order to avoid the effect of colour sample , the sample is compared against a
filtered portion of the same water. The method has been calibrated with recognised formazin turbidity solutions. The turbidity is
expressed in terms of Formazin Turbidity Units (FTU). These units are equivalent to Jackson Turbidity Units(JTU) and Nephelometric
Turbidity Units (NTU)
49
REAGENTS AND EQUIPENT
Filtration Kit (cono-luer lock syringe+10 syringe filters). Code 1.9594.01 / Round cuvette 16 mm. Ø with cap (4pcs). Code 1.9365.00
(cuvette used in the test) / DINKO D-101 Photometer, use the calibration chart.
DINKO D-105 Photoanalyzer and D-100 Photometer, select program nr. 53
PROCEDURE
1. - Filter a portion of sample through a syringe filter system
2. - Fill a test tube with filtered sample and retain for use as the blank tube.
3. - Fill a test tube with unfiltered sample to 10 ml mark.
4. - Select 520 nm filter on photometer.
5. - Take photometer reading. Use the filtered sample as the zero.
6. - Consult calibration chart (D-101 Photometer). Select program nr. 53 (D-100 and D-105 Photometers).
Range: 5 - 400 FTU Turbidity 520 nm

ABS 0 1 2 3 4 5 6 7 8 9
0.0 0 3 7 11 14 18 22 25 29 33
0.1 36 40 43 47 51 54 58 62 65 69
0.2 72 76 80 84 89 93 98 103 107 112
0.3 116 121 125 129 132 136 140 144 148 152
0.4 156 160 163 167 171 174 178 182 186 189
0.5 193 197 200 205 209 213 218 222 226 231
0.6 235 239 244 248 252 257 261 265 270 274
0.7 278 283 287 291 295 300 304 308 313 317
0.8 321 326 330 334 339 343 347 352 356 360
0.9 365 369 373 382 386 390 395 400
Optionally, a light cover is provided to cover the cuvette and reduce the light in very bright outdoor environments.
It is not necessary to use it indoors.
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is ± 4,700 NTU
Traceability: The traceability of the method of settles down with SM “Standard Methods of Water and Wastewater, APHA-AWWA-
WOPC F, Edition 16 and 17, adapted for photometric reading.
ZINC. Code 1.9411.00 (50t) - 1.9499.00 (250t) Photometer Method 620-630 nm

Test fo Zinc in natural and treated water 0- 4,0 mg / l
Zinc compounds are used as corrosion inhibitors in industrial cooling water systems. The Zinc level in such systems is important.
The DINKO Zinc test provides a simple means to control the Zinc level over the range of 0 a 4 mg/l. in industrial effluents and natural or
drinking waters
METHOD
Zinc reacts with 5-(o- carboxyphenyl)-1-(2-hydroxi-5-sulphoophenyl)-3-phenilformazan ( Zincon) in alkaline solution to give a intense
blue colour. The reagent itself is orange in solution. At different zinc levels a distinctive colour range from orange through purple to blue
is produced. In the test a tablet reagent containing both Zincon and an alkaline buffer is used for maximum convenience. The test is
carried out by adding a tablet to the sample of the water. Samples containing high chlorine residuals are pre-treated with a special
dechlorinating tablet to prevent bleaching of the test colours.
The colour produced in the test is indicative of the Zinc concentration and is measured using a DINKO Photometer.
Copper reacts in similar manner to Zinc and a correction procedure using EDTA is applied to those which contain both zinc and copper.
EDTA destroys the colour complex formed with Zinc.

Zinc Tablet / Zinc / Dechlor Tablet / EDTA Tablet /Round cuvette 16mm Ø with cap.( 4pcs ). Code 1.9365.00 (cuvette used in the chart)
Square cuvette 10 mm with cap. (100pcs). Code: 1.9363.00 / Photometer DINKO D-101, use the calibration chart, filter 620 nm
Photoanalyzer DINKO D-105 and D-100 Photometer select program nr. 51
PROCEDURE
2. - IN THE CASE OF CHLORINE CONTAINING SAMPLE ONLY. Add one Zinc-Dechlor tablet, crush and mix to dissolve.
3. - Add one Zinc tablet, crush and mix to dissolve.
4. - Stand for five minutes then mix again to ensure complete dissolution of the indicator.
5. - Select wavelength 620 or 630nm on the Photometer.
6. - Take Photometer reading (see Photometer Instructions). Make blank without tablets.
7. - Consult calibration chart ( D-101). Select program 51 (D-105 and D-100)
8. - FOR COPPER CONTAINING SAMPLE ONLY.
Continue the test on the same test portion. Add one EDTA tablet, crush and mix to dissolve.
9. - Take Photometer reading or consult calibration chart. Then subtract the value obtained from the Zinc concentration
previously noted. This gives the corrected Zinc concentration.
50
Range: 0-4,0 mg / L Zn 620 nm
ABS 0 1 2 3 4 5 6 7 8 9
0.0 0.00 0.02 0.05 0.08 0.11 0.13
0.1 0.16 0.18 0.21 0.24 0.26 0.29 0.31 0.34 0.36 0.39
0.2 0.42 0.44 0.47 0.49 0.52 0.55 0.57 0.60 0.62 0.65
0.3 0.68 0.70 0.73 0.75 0.78 0.80 0.83 0.86 0.88 0.91
0.4 0.93 0.96 0.99 1.01 1.04 1.06 1.09 1.11 1.14 1.17
0.5 1.19 1.22 1.25 1.28 1.31 1.33 1.36 1.39 1.42 1.45
0.6 1.48 1.51 1.53 1.56 1.59 1.62 1.65 1.68 1.71 1.73
0.7 1.76 1.79 1.82 1.85 1.88 1.91 1.93 1.96 1.99 2.02
0.8 2.05 2.08 2.11 2.15 2.18 2.21 2.24 2.27 2.30 2.33
0.9 2.36 2.39 2.42 2.45 2.48 2.52 2.55 2.58 2.61 2.64
1.0 2.67 2.70 2.73 2.76 2.79 2.83 2.87 2.90 2.94 2.98
1.1 3.02 3.05 3.09 3.13 3.17 3.20 3.25 3.29 3.34 3.38
1.2 3.42 3.47 3.51 3.55 3.60 3.64 3.69 3.73 3.77 3.82
1.3 3.86 3.90 3.95 4.00
Uncertainty: The uncertainty associated to the calibration for a constant of trust K=2 is of ± 0,043mg/L Zn
Traceability: The traceability of the method settles down with “ Federal Register” 42 (105)36166
Alkaphot®, Calcicol®, Coppercol®, Hardicol®, Magnecol®, Nitratest®, Nitricol® y Nitriphot® They are trademarks of Palintest Ltd.
51
OTHER DINKO APPARATUS
- Blenders-Homogenizers - Peristaltic Pumps
-Colorimeters - pH-meters
- Conductimeters - Photometers
- Extractor for mince analysis - Proportioner Pumps
- Heater Metallic Blocks - Rotary Stirrers
- Heater Plates - Rod Stirrers
- Infrared Ovens - Sand Baths
- Kits for Water Analysis - Spectrophotometer
- Magnetic Stirerrs - Temperature Controllers
- Microscopes - Timers
- Moisture Analyze - Triquinoscopes
- Nephelometers - Turbidimeters
- Orbital Shakers - Turn Dishes
- Oxygen Meters - Vacuum Pump
DINKO Instruments
DINTER, S. A. Encarnació, 123-125. Phone. +34 93 284 69 62 Fax +34 93 210 43 07/ 08024-Barcelona
e-mail: dinter@dinko.es www.dinko.es
52

Manual Photometric Methods For Water Analisys 82325

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DINKOTEST

Manual of Photometer Methods

Photometer DINKO D-101 Digital reader AB - T% - PPM

Photometer DINKO D-100 Digital reader T% - PPM direct - Memory

Photoanalyzer DINKO D-105 Digital reader AB - T% - PPM direct

Kits for quick analysis of water

The absorbance (A) is defined as : A = log ( 1 / T )

Therefore the Absorbancia is directly proportional to the concentration.

This lineal relationship is known as the “Law of Beer", see fig. 2.

Fig. 2 “ Law of Beer “

Range: 0- 5.0 mg / L Fe Iron 520 nm

Dilutions and ranges

Bigger dilutions would be:

ALKALINITY M. Code 1.9404.00 (50t) Photometer Method 577-580 nm

REAGENTS AND EQUIPMENT

1. - To filter the sample if it is necessary to obtain a clear solution.

1.- If Alkalinity P = 0 2. - If Alkalinity P > 0 and M > 2P

2. – If Alkalinity P > 0 and M < 2P

Where M and P represent the values of alkalinity M and alkalinity P respectively.

ALKALINITY P. Code 1.9405.00 (50t) Photometer Method 520 nm

REAGENTS AND EQUIPMENT

ALUMINIUM. Code 1.9401.00 (50t) - 1.9469.00 (250t) Photometer Method 580-577 nm

REAGENTS AND EQUIPMENT

REAGENTS AND EQUIPMENT

RANGE: 0 - 15 mg/L N Ammonia Nitrogen mg/L N 420/415 nm

REAGENTS AND EQUIPMENT

SEA WATERS SAMPLES

RANGE: 0-1.0 mg / L N PPM AMMONIA N 620 nm

BROMINE. Code 1.9409.00 (50t) - 1.9472.00(250t) Photometer Method 520nm

REAGENTS AND EQUIPMENT

TEST PROCEDURE-FREE AND COMBINED BROMINE

Free Bromine = Total Bromine - Combined Bromine

Range: 0-10,0 mg / L Bromine 520 nm

REAGENTS AND EQUIPMENT

where dH = german hardness ; eH = english hardness ; fH = french hardness.

RANGE: 0- 500 mg/L Ca CO3 Calcium, Hardness 580 nm

CHLORIDE. Code 1.9419.00(50t) - 1.9475.00 (250t) Photometer Method 520 nm

REAGENTS AND EQUIPMENT

For Testing Natural Waters, Swimming Pools and Boiler Waters

For Testing Salt Chlorinator Treated Swimming Pools

For Testing Sea Water and Brackish Waters

REAGENTS AND EQUIPMENT

Combined Chlorine = Total Chlorine - Free Chlorine

Range: 0 - 5 mg / L Chlorine 520 nm

REAGENTS AND EQUIPMENT

Range: 0 - 250 mg / L Chlorine 490 nm

REAGENTS ANDY EQUIPMENT

Range: 0- 5,0 mg / L 520 nm

PROCEDURE – FREE AND COMBINED CHLORINE, AND CHLORITE

COLOUR. Code1.9423.00 (50t) Photometer Method 415 - 420 nm

Range: 0 - 500 mg / L Pt 420 nm

COPPER. Code 1.9422 (50t) - 1.9476.00 (250t) Photometer Method 520 nm

REAGENTS AND EQUIPMENT

Range: 0 - 5,0 mg / L Cu Copper 520 nm

CHEMICAL OXYGEN DEMAND - COD/ 150. Photometer Method 440-450 nm

REAGENTS AND EQUIPMENT

REAGENTS AND EQUIPMENT

RANGE: 100 - 2000 mg / L O2 PPM Oxygen 577 - 580nm

RANGE: 1000 - 20000 mg / L O2 PPM Oxygen 577- 580 nm