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    MagNA Pure 24 Protocols

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    Mỹ Dung

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    MagNA Pure 24 Protocols

    Uploaded by

    Mỹ Dung
    2 views1 page

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    © © All Rights Reserved

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    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    2 views1 page

    MagNA Pure 24 Protocols

    Uploaded by

    Mỹ Dung

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 1

    MagNA Pure 24 System protocols

    Input sample type and sample volumes Run


    Elution
    Target Protocol name time***
    Whole Nasal Cultured Fresh-frozen volume
    Plasma Serum BAL** Urine Stool FFPET † (h:mm)
    blood swabs* cells tissue
    Pathogen 200
    1:22
    Protocol optimized for speed

    Fast Pathogen 200 200 µL


    8 sample protocol optimized 0:35
    for speed, yield and purity
    Bacterial, fungal, Pathogen 1000
    and viral NA Protocol optimized for yield 500 or 1000 µL 1:40
    and purity

    External Lysis Pathogen 200 450 μL lysate from 1:30


    External Lysis Pathogen 500 200 μL sample 50 or
    Protocols optimized with 1450 μL lysate from 100 µL
    external lysis buffer 500 μL sample 1:40

    hgDNA 200
    Protocol optimized for Up to 5mg 1:15
    human genomic NA
    200 μL
    Fast hgDNA 200
    (≤2 × 106
    8 sample protocol optimized 0:25
    cells)
    for speed Up to
    hgDNA ds 200 5 x 10 5
    1:15
    Protocol optimized for NGS
    Genomic DNA
    500 μL
    (≤5 × 106
    hgDNA 1000
    cells) 100 or
    Protocol optimized for yield 2:25
    1000 μL 200 µL
    and purity Up to
    (≤1 × 107
    1 x 106
    cells)

    DNA FFPET 1000 Up to 6 x 5


    50 or
    Deparaffinization μm sections 5:30
    100 µL
    and lysis on board (up to 6 mm3)

    cfNA ss 2000 2:05


    2000 µL
    cfNA ss 4000 50 or
    Protocols optimized for 100 µL
    4000 µL 3:00
    single-stranded DNA

    100,
    cfNA ds 2000 2:30
    Cell-free NA 2000 µL 150,
    cfNA ds 4000
    or 200
    Protocols optimized for
    4000 µL µL 3:30
    double-stranded DNA

    cfNA ds 4000 hp
    60 or
    Protocol optimized for dou- 4000 µL 4:40
    150 µL
    ble -stranded DNA and NGS
    200 µL
    Sample Transfer to the
    500 µL 0:08
    processing cartridge 1000 µL
    PCR Setup for 2-25 µL per
    eluate for PCR Setup in Frame-
    0:05
    Post elution Strip with flat caps-Low Profile
    or LightCycler® 8-tube strips

    Archiving for 25-50 µL


    per eluate FrameStrip low
    0:05
    profile strips, or LightCycler®
    8-tube strips

    New protocol
    *Nasopharyngeal/nasal swabs
    **BAL - Bronchoalveolar lavage
    ***These times are approximate and should only be used as a guidance.
    †
    FFPET - formalin-fixed paraffin-embedded tissue

    All MagNA Pure 24 kits, consumables, and accessories are for in vitro diagnostic Published by
    unless otherwise noted. Roche Molecular Systems, Inc

    Start here. Go Anywhere. MAGNA PURE and LIGHTCYCLER are registered trademarks of Roche.
    All other product names and trademarks are the property of their respective owners.
    4300 Hacienda Drive
    Pleasanton, CA 94588 USA

    ©2020 Roche Molecular Systems, Inc. All rights reserved. PP-GBL-00834 02.2020

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