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    molecular bio.TY

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    shubham sonawane

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    © All Rights Reserved
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    molecular bio.TY

    Uploaded by

    shubham sonawane
    1 views2 pages

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    1 views2 pages

    molecular bio.TY

    Uploaded by

    shubham sonawane

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    Course Code: ZO 363

    Credits: 02

    ZO 363 - Molecular Biology

    Objectives:

    1. The course aims to provide students with an introduction of the underlying molecular
    mechanisms of various biological processes in cells and organisms.

    2. To understand the Structure of DNA and RNA, DNA and RNA as genetic material

    3. To understand the Central Dogma of Molecular Biology

    4. To understand the concept of gene regulation

    5. To understand the DNA Damage and Repair

    6. The course aims to develop basic understanding of structure-function relationships of nucleic


    acids and proteins.

    Learning outcomes:

    1. Learner shall get an insight into molecular mechanisms of various biological processes in cells and
    organisms

    2. Learner shall get an insight into the Structure of DNA and RNA, DNA and RNA as genetic material

    3. The course shall prepare learner to get insight into the Central Dogma of Molecular Biology

    CBCS: 2021-20222 T. Y. B. Sc. Zoology

    Savitribai Phule Pune University Page 27

    4. Learner shall also understand the concept of gene regulation

    5. Learner shall get an insight into the DNA Damage and Repair

    Title & Contents

    Number of lectures

    1. Nucleic Acids and Chromatin:

    1.1 Structure of RNA & DNA.

    1.2 Types of RNA.

    1.3 DNA as genetic material - evidences (Griffith’s, Avery et al., Hershey

    and Chase experiment), RNA as genetic material - TMV 4.

    1.4 Structure of Chromatin, packaging of DNA, Heterochromatin,


    Euchromatin.

    7L

    2. Central Dogma of Molecular Biology:

    2.1 DNA Replication - Semiconservative (Messelson and Stahl

    experiment), Basic mechanism of replication in

    prokaryotes and eukaryotes.

    2.2 Transcription -

    2.2.1 Basic mechanism of transcription in prokaryotes and eukaryotes,

    RNA polymerase enzyme in prokaryotes.

    2.2.2 RNA modifications and processing (splicing - mRNA,

    modifications at 3’and 5’ end).

    2.3 Translation - Genetic code, properties of genetic code, Basic

    mechanism of Translation in E. coli and eukaryotic cells.

    15 L

    3. Lac operon:

    1L

    4. DNA repair mechanism:

    Photo repair, dark repair, base excision repair.

    3L

    5. Recombinant DNA Technology:

    Introduction, restriction enzymes, cloning vector, PCR (polymerase chain

    reaction), DNA finger printing.

    4L

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