Bio Fertilizer

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RAJMATA VIJAYARAJE SCINDIA KRISHI VISHWA VIDYALAYA, GWALIOR

COLLEGE OF AGRICULTURE INDORE

SESSION: 2021-2022

Class: B.Sc (hons.) Ag 3rd year 1st semester


Subject: Bio-pesticides and Bio-fertilizers

Topic: Structure and characteristic features of bacterial biofertilizers-azospirillium,,

azotobacter, bacillus, pseudomonas, rhizobium and frankia, cynobacterial

course teacher: dr. Anusuiya panda


dr. Neelesh raypuriya
GROUP MEMBERS

1 ANKIT PARMAR (19120111)


2 ANSHUL SHARMA (19120112)
3 ANURAG KUMAR JAITWAR (19120113)
4 ASHISH BIRLA (19120114)
5 ASHUTOSH SINGH (19120115)
6 ASLAM KHAN MANSOORI (19120116)
7 BALRAM CHANDEL (19120117)
8 DEEPU PRAJAPATI (19120118)
9 DESHIE CHOUBEY (19120119)
10 DEVENDRA (19120120)
INDEX:

1 INTRODUCTION
2 BENEFITS AND TYPES
3 BACTERIAL BIOFERTILIZER
4 STRUCTURE AND CHARACTERISTIC FEATURES OF BACTERIAL
BIOFERTILIZERS
5 CONCLUSION
6 REFERENCES
INTRODUCTION

• A biofertilizer is a substance which contains living micro-organisms which, when applied to seeds, plant
surfaces, or soil, colonize the rhizosphere or the interior of the plant and promotes growth by increasing the
supply or availability of primary nutrients to the host plant

• Biofertilizers add nutrients through the natural processes of nitrogen fixation, solubilizing phosphorus, and
stimulating plant growth through the synthesis of growth-promoting substances.

• The microorganisms in biofertilizers restore the soil’s natural nutrient cycle and build soil organic matter.

• Through the use of biofertilizers, healthy plants can be grown, while enhancing the sustainability and the
health of the soil.
BENEFITS FROM USING BIO FERTILIZERS

•  Increase crop yield by 20-30%.


• Replace chemical nitrogen and phosphorus by 25%.
• Stimulate plant growth.
• Activate the soil biologically.
• Restore natural soil fertility.
• Provide protection against drought and some soil borne diseases
AZOSPIRILLIUM

Mainly present in cereal plants and


grasses.
Inhabit both root cells and
surrounding of root as well.
Form symbotic relation(endo-
symbioant) with plant through which
nitrogen fixing potential of plant
increases.
• Nitrogen is required in the range of 15-30kg/ha in non-
leguminous plant such as cereals,oilseed,etc.
• Considerable quantity of nitrogen fertilizer upto 25-30% can be
saved by adding azospirillium inoculant.
• It also benefits the host plant by supplying growth hormone and
vitamins.
• It has good adaptability to temperature, soil PH and wide host
range.
FRANKIA

• Frankia is the genus of N2 fixing actinomycetes capable of fixing N2.


• Fix nitrogen as free living bacteria and in plant nodules unlike rhizobium.
• Form nodules similar to those of rhizobium legume symbiosis.
• It fixes nitrogen in non-leguminous plants.
• Frankia can fix n2 at normal o2 concentration at the rate sufficient to support its
growth.
• N2 fixation is accompanied by the
development of terminal swelling
known as vesicles.
• Vesicles protects the niteogenase
enzyme from o2 poisining.
• Also very important in c cycling.
RHIZOBIUM
• Rhizobium definition
• “Rhizobium is a soil bacteria that fixes atmospheric nitrogen once it finds a base inside the roots of  the
leguminous plants
• Rhizobium is the bacteria that live in symbiotic association with the root nodules of the leguminous plants.
• Fixation of nitrogen cannot be done independently. That is
why rhizobium requires a plant host.
• Rhizobium is a vital source of nitrogen to agricultural soils
including those in arid regions. They convert dinitrogen into
ammonia.
• Ammonia, being toxic in nature. Is rapidly absorbed into organic compounds.
Classification of Rhizobium Bacteria
Rhizobium can be classified on the basis of the types of the
plant they are associated with and also the rate of growth.
Few species of Rhizobium bacteria include:
•Rhizobium leguminosarum
•Rhizobium alamii
•Rhizobium lantis
•Rhizobium japonicum
•Rhizobium trifolii
CYANOBACTERIA
• They show great diversity in form and shape. Some are spherical; some are rod-shaped, while few of
them are unicellular or multicellular.
• They are omnipresent, and occur in all possible kinds of habitats.
• The pigments found in this group are-chlorophyll a, p-carotene,
antheraxanthin, aphanicin, aphanizophyll, flavacin, lutein, myxoxanthophyll etc. 
• The storage products are cyanophycean starch and protein.
• The flagella are absent.
• They may be unicellular (e.G., Chroococcus, tetrapedia, gloeocapsa), 
• Colonial (e.G., Aphanocapsa, nostoc, aphanothece) and 
• Filamentous (e.G., Oscillatoria).
• They are found in soil, clean water, and marine environment.
• Some forms can live symbolically.
• Some have the capacity to fix atmospheric nitrogen.
CELL STRUCTURE OF
CYANOBACTERIA :-

Cyanobacteria are prokaryotes


with cell envelopes typical for
Gram-negative bacteria. The cell
envelope consists of four distinct
layers, the plasma membrane, the
peptidoglycan layer, the outer
membrane and in some cases the
surface or S-layer. Often, the latter
three are referred to as the cell
wall.
PSEUDOMONAS

A large group of aerobic, non sporing gram negative bacteria motile by polar flagella

found I nature water, soil, other moist environments

some of them are pathogenic to plants


 creation of new genera such as burkholderia. Stenotrophomnonast
MORPHOLOGY

They are slender gram negative bacillus, 1.5-3 microbes x


0.5

microns monoflgellar?

Non capsulated but many strains have mucoid slime layer

isolates from cystic fibrosis patients have abundance of


extracellular polysaccharides composed of alginate
polymers

• escape the defence mechanisms by loose capsule in


which micro colonies of bacillus are enmeshed and
protected from host defences.
AZATOBACTOR

• Azotobactor is a heterotrophic free living nitrogen fixing


bacteria present in alkaline and neutral soils.
• Azotobactor is the most commonly occurring species in
arable soils of india.
• Apart from its ability to fix atmospheric nitrogen in soils,
it can also synthesize growth promoting substances such
as auxins and gibberellins and also to some extent the
vitamins.
•  Many strains of azotobactor also exhibit fungicidal properties against certain
species of fungus.
• Response of azotobactor has been seen in rice, maize, cotton, sugarcane, pearl
millet, vegetable and some plantation crops.
• It improves seed germination and plant growth.
• Azotobacter is heaviest breathing organism and requires a large amount of
organic carbon for its growth.
BACILLUS 

• Bacillus is a nitrogen fixation bacteria


• Non- symbiotic
• aerobic
• Rods shaped,
• This is belong to bacillacea family
• endospore-forming genus Bacillus is a systematically diverse taxon (Claus and
Berkeley 1986).
• The three former Bacillus species,
Paenibacillus azotofixans,
Paenibacillus macerans and P.
polymyxa,
• were nitrogen fixers, based on
nitrogenase activity
• .In the current study, isolates of
nitrogen-fixing bacilli from plant
rhizospheres. This report shows that
nifH gene exists in the both genera
Bacillus and Paenibacillus.
CONCLUSION
• Biofertilizers increase the availability of plant nutrients.

• Maintenance of the soil fertility over a long period.


• Some microorganisms have the beneficial role of biological nitrogen fixation to supply nitrogen to crops
• Solubilizing insoluble phosphates to plant-available (soluble) forms and synthesizing biomass for manuring
of crops like rice.
• Biofertilizers are, therefore, economical, renewable and eco-friendly, but they cannot totally replace
chemical fertilizers.
• Biofertilizer use is an important component of integrated nutrient management and organic farming.

• These technologies are becoming vital in modern-day agricultural practices. The changing scenario of
agricultural practices and environmental hazards associated with chemical fertilizers demand a more
significant role of biofertilizers in coming years.
REFERENCE

• Biological nitrogen fixation and biogas technology 


• Manures fertilizers & agrochemicals
• ORGANIC FARMING :: biofertilizers technology (tnau.Ac.In)
THANK YOU

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