Enzymes PP

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Review of Biochemistry

Organic vs. Inorganic


Organic Compounds
1. Proteins
2. Carbohydrates
3. Lipids
4. Nucleic Acids
WHAT DO ENZYMES
DO?
Enzymes greatly
increase the rate at
which reactions take
place
WOW!
That reaction is really
fast!
Enzymes
• Enzymes are proteins

• Enzymes are very specific


– One enzyme, One function!

• The substance that an enzyme changes is


called a substrate.
SOME IMPORTANT THINGS TO
KNOW ABOUT ENZYMES:

• Each chemical reaction requires a


specific enzyme
• Enzymes can be used over and
over again - Recycle!!
• Example: digestion of food
Other Interesting Enzyme Facts:

• They are made of protein

• They work because they have a


specific shape…works like a lock and
key!
Enzyme
Reactions
• Digestion- Breaking down
molecules

• Synthesis- Building up
molecules
How Enzymes Work

• On the surface of the enzyme is a region called


an active site.

• The substrate will bond to the active site of the


enzyme, then break into smaller “pieces”.

• Works like a “lock and key”.


AN ENZYME REACTION:
WHEN DOES THE ACTUAL
REACTION OCCUR?

When the substrate


binds to the
enzyme…A.K.A.

THE ENZYME-
SUBSTRATE
COMPLEX
Naming
Enzymes
•Add -ase to the end
of the substrate
Substrate = maltose

Enzyme = maltase
More Examples:
Factors
Influencing
Enzyme
Action
Factors Affecting Enzyme
Action
• 1) Amount and concentration of
enzyme/substrate

• 2) Extreme Temperature

• 3) pH – Too acidic or basic


Factors Affecting Enzyme Action
Temperature
Optimum Temperature
Enzyme
Activity

10 20 30 40
Temperature
pH
Optimum pH
Enzyme
Activity

1 2 3 4 5 6 7 8 9 10 11 12 13 14
pH
Enzyme Concentration

Enzyme
Activity

Little A lot Tons


Enzyme Concentration
Oh No!
I’m out
numbered

Ninja Bad
-Enzyme Guys
-Substrate
Now, We’re in
trouble! There
are more
Ninjas
Substrate Concentration

Enzyme
Activity

Little A lot Tons


Substrate Concentration
Denaturation- an enzyme shape is
changed and can no longer do its
job.
Enzyme Haiku
Enzymes do cool stuff
They increase reaction rates
Without them, we’d die

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