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Water Sampling Methods For Lab Analysis

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25 views10 pages

Water Sampling Methods For Lab Analysis

Uploaded by

quality.mpenv
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Lecture - 1

Water Sampling for Lab


Analysis
Main objective of water sampling:
The main objective of sampling is to collect a portion of material small
enough in volume to be transported conveniently and yet large enough
for analytical purposes while still accurately representing the material
being sampled.
Objectives of water sampling for lab analysis in detail:
 To use reliable and useful source for sample collection
 To determine the quality of nature in its natural state
 To assess the effect of human activities on water quality and its
sustainability
 To keep under observations the sources and pathways of pollutants /
contaminants
Requirements for Sampling:
• Meet the requirements of the sampling program.
• Handle the sample carefully so that it does not deteriorate or become
contaminated or compromised before it is analyzed.
• Ensure sampling all equipment are clean and quality assured before use.
• Use sample containers that are clean and free of contaminants.
• Rinse the bag/bottle at least twice with the sample water prior to filling and
closing.
Requirements for Sampling (contd.):
• Fill bag/bottle as full as possible. Half-filling leaves more room for oxygen
which will promote degradation of your sample.
• If sampling a body of running water, point the mouth of the bag upstream
and your hands downstream to avoid contamination.
Requirements for Sampling (contd.):
• If sampling from a water faucet, run the faucet for 1 minute before obtaining
a sample.
• Make records of every sample collected and identify every bottle e.g., take
notes and photographs, fill out tags, etc.
• Place the sample into appropriate, labeled containers.
• All samples must be preserved as soon as practically possible.
 Sampling of Water for Analysis:
A common cause of error in water quality analysis is improper sampling.
The results of a water quality analysis of a sample show only what is in the
sample. For the results to be meaningful, the sample must be representative
i.e., it must contain essentially the same constituents as the body of water
from which it was taken.
 Sample Collection bottles, Size and Materials:

The methods that will be followed will determine the type of bottles used. For
example,

 Samples for metals’ analyses are usually collected in plastic bottles

 Analyses for volatile organics and pesticides are collected in glass containers.
Bottles used to collect samples for bacteria should be sterilized.

 Certain analysis like volatile organics and radon require vials that are to be
filled leaving no head space, which keeps these analytes dissolved in the
water preventing them from escaping into the air.
 Sample Collection bottles, Size and Materials (contd.):

 Additionally, some analyses require samples to be collected in amber colored


bottles. These darker bottles are for analytes (polypropylene and LDPE) that
break-down in sunlight, which helps keep these contaminants from breaking
down while in transit to the laboratory for analysis.

 The size of the container is important to ensure enough sample to run the
analysis needed.
Figure: Polypropylene Figure: Schematic of LDPE
branching structure

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