Next Generation Sequencing
Next-generation sequencing, also known as massively parallel sequencing, is the term used to describe the modern sequencing technology. Next-generation sequencing is capable to sequencing millions of copies of DNA fragments in a single reaction.
UK HealthCare Genomics Core Laboratory has five Illumina® next-generation sequencing systems, the NovaSeq 6000, two NextSeq 2000, and two MiSeq benchtop sequencers. The Illumina sequencing systems utilize sequencing by synthesis (SBS) technology and support both single-read and paired-end libraries.
In addition to sequencing services, UK Genomics Core Laboratory offers select library preparation services.
Sample Submission Instructions
For inquiries or sample submission instructions, contact us at genomics@uky.edu. For directions, please see our About Us page.
NGS Research Services Pricing, July 2022-June 2023
| Sequencing Service | Read Pairs (M)† | Output (Gb)† | UK Price* | External Price* |
| NovaSeq S4 (300 cycles) | 10,000 | 3000 | $17,132 | $ 18,845.20 |
| NovaSeq S4 (200 cycles) | 10,000 | 2000 | $15,393 | $ 16,932.30 |
| NovaSeq S4 (35 cycles); 1x35 | 10,000 | 350 | $12,543 | $ 13,797.30 |
| NovaSeq S2 (300 cycles) | 4,100 | 1250 | $11,474 | $ 12,621.40 |
| NovaSeq S2 (200 cycles) | 4,100 | 833 | $10,767 | $ 11,843.70 |
| NovaSeq S2 (100 cycles) | 4,100 | 417 | $8,704 | $ 9,574.40 |
| NovaSeq S1 (300 cycles) | 1,600 | 500 | $6,346 | $ 6,980.60 |
| NovaSeq S1 (200 cycles) | 1,600 | 333 | $5,875 | $ 6,462.50 |
| NovaSeq S1 (100 cycles) | 1,600 | 167 | $4,696 | $ 5,165.60 |
| NovaSeq SP (500 cycles) | 800 | 400 | $5,109 | $ 5,619.90 |
| NovaSeq SP (300 cycles) | 800 | 250 | $3,694 | $ 4,063.40 |
| NovaSeq SP (200 cycles) | 800 | 167 | $3,400 | $ 3,740.00 |
| NovaSeq SP (100 cycles) | 800 | 80 | $2,635 | $ 2,898.50 |
| NextSeq 2000 P3 (300 Cycles) | 1,200 | 360 | $6,562 | $ 7,218.20 |
| NextSeq 2000 P3 (200 Cycles) | 1,200 | 240 | $4,955 | $ 5,450.50 |
| NextSeq 2000 P3 (100 Cycles) | 1,200 | 120 | $3,616 | $ 3,977.60 |
| NextSeq 2000 P3 (50 Cycles); 1x50 | 1,200 | 60 | $2,545 | $ 2,799.50 |
| NextSeq 2000 P2 (300 Cycles) | 400 | 120 | $3,927 | $ 4,319.70 |
| NextSeq 2000 P2 (200 Cycles) | 400 | 80 | $2,995 | $ 3,294.50 |
| NextSeq 2000 P2 (100 Cycles) | 400 | 40 | $1,656 | $ 1,821.60 |
| NextSeq 2000 P1 (300 Cycles) | 100 | 30 | $1,441 | $ 1,585.10 |
| MiSeq (600 Cycles) (v3) | 25 | 15 | $2,051 | $ 2,256.10 |
| MiSeq (150 Cycles) (v3) | 25 | 3.8 | $1,272 | $ 1,399.20 |
| MiSeq (500 Cycles) (v2) | 15 | 8.5 | $1,608 | $ 1,768.80 |
| MiSeq (300 Cycles) (v2) | 15 | 5.1 | $1,445 | $ 1,589.50 |
| MiSeq (50 Cycles) (v2) | 15 | 0.85 | $1,160 | $ 1,276.00 |
| MiSeq Micro (300 Cycles) (v2) | 4 | 1.2 | $693 | $ 762.30 |
| MiSeq Nano (500 Cycles) (v2) | 1 | 0.5 | $578 | $ 635.80 |
| MiSeq Nano (300 Cycles) (v2) | 1 | 0.3 | $505 | $ 555.50 |
Contact us at genomics@uky.edu
Visit our website
*Prices listed are non-binding and subject to change. List price includes loading/pool QC (TapeStation and Qubit).
†Maximum run output, based on Illumina advertised specifications and is not guaranteed. Output may vary with library type and quality, base diversity, and more.