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    Mcqs - Biochemistry

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    nagendra_rd
    This document contains 15 multiple choice questions about High Performance Liquid Chromatography (HPLC). HPLC is a technique used to separate, identify, and quantify compounds in a mixture. The questions cover topics such as the definition of terms used in HPLC like eluent strength, isocratic and gradient elution, and characteristics of normal and reversed phase HPLC. The document provides the questions, possible answers for each, and a link to an online forum discussing HPLC.

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    © All Rights Reserved
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    Mcqs - Biochemistry

    Uploaded by

    nagendra_rd
    1K views3 pages
    This document contains 15 multiple choice questions about High Performance Liquid Chromatography (HPLC). HPLC is a technique used to separate, identify, and quantify compounds in a mixture. The questions cover topics such as the definition of terms used in HPLC like eluent strength, isocratic and gradient elution, and characteristics of normal and reversed phase HPLC. The document provides the questions, possible answers for each, and a link to an online forum discussing HPLC.

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    MCQ on Chromatography

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    This document contains 15 multiple choice questions about High Performance Liquid Chromatography (HPLC). HPLC is a technique used to separate, identify, and quantify compounds in a mixture. The questions cover topics such as the definition of terms used in HPLC like eluent strength, isocratic and gradient elution, and characteristics of normal and reversed phase HPLC. The document provides the questions, possible answers for each, and a link to an online forum discussing HPLC.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    1K views3 pages

    Mcqs - Biochemistry

    Uploaded by

    nagendra_rd
    This document contains 15 multiple choice questions about High Performance Liquid Chromatography (HPLC). HPLC is a technique used to separate, identify, and quantify compounds in a mixture. The questions cover topics such as the definition of terms used in HPLC like eluent strength, isocratic and gradient elution, and characteristics of normal and reversed phase HPLC. The document provides the questions, possible answers for each, and a link to an online forum discussing HPLC.

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    © All Rights Reserved
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    12/20/2016

    MCQsBIOCHEMISTRY:HPLC|PFMSGFORUM
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    BIOCHEMISTRY: HPLC

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    1.
    HPLCstandsfor

    aayisha quddus
    Member
    Reputation: 13

    A.HighPressureLiquidChromatography
    B.HighPerformanceLiquidChromatography
    C.both(a)and(b)
    D.HighlyPlacedLiquidChromatography
    Answer&Explanation
    Answer:OptionC
    2.
    Theeluentstrengthisameasureof
    A.solventadsorptionenergy
    B.solventabsorptionenergy
    C.solventdiffusivity
    D.solventmixingindex
    Answer&Explanation
    Answer:OptionA
    2.
    Theeluentstrengthisameasureof
    A.solventadsorptionenergy
    B.solventabsorptionenergy
    C.solventdiffusivity
    D.solventmixingindex
    Answer&Explanation
    Answer:OptionA
    3.
    HPLCmethodsinclude
    A.liquid/liquid(partition)chromatography
    B.liquid/solid(adsorption)chromatography
    C.ionexchangeandsizeexclusionchromatography
    D.alloftheabove
    Answer&Explanation
    Answer:OptionD
    4.
    Columnefficiencyismeasuredintermsofnumberofplateswhichis
    A.inverselyrelatedtothesquareofthepeakwidth
    B.directlyrelatedtothesquareofthepeakwidth
    C.inverselyrelatedtothecuberootofthepeakwidth
    D.directlyrelatedtothesquareofthepeakwidth
    Answer&Explanation
    Answer:OptionA
    5.
    Foratypicaladsorbentsuchassilicagel,themostpopularporediametersare
    A.10and50A
    B.60and100A

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    MCQsBIOCHEMISTRY:HPLC|PFMSGFORUM
    C.100and150A
    D.150and200A
    Answer&Explanation
    Answer:OptionB
    6.
    AnisocraticelutioninHPLCisoneinwhichthecompositionofthesolvent
    A.remainsconstant
    B.changescontinuously
    C.changesinaseriesofsteps
    D.noneofthese
    Answer&Explanation
    Answer:OptionA
    7.
    InreversedphaseHPLC,thereisa
    A.nonpolarsolvent/polarcolumn
    B.polarsolvent/nonpolarcolumn
    C.nonpolarsolvent/nonpolarcolumn
    D.anyoftheabove
    Answer&Explanation
    Answer:OptionB
    8.
    WhichofthefollowingstatementsistrueforarefractiveindexdetectorinHPLC?
    A.ItismoresensitivethanaUVdetector
    B.Itcanonlybeusedforisocraticelutions
    C.Itdoesnotrespondtomanysolutes
    D.noneofabove
    Answer&Explanation
    Answer:OptionC
    9.
    AgradientelutioninHPLCisoneinwhichthecompositionofthesolvent
    A.remainsconstant
    B.ischangedcontinuouslyorinaseriesofsteps
    C.both(a)and(b)
    D.noneoftheabove
    Answer&Explanation
    Answer:OptionB
    10.
    Aneluotropicseries
    A.rankssolventsbytheirrelativeabilitiestodisplacesolutesfromagivenabsorbent
    B.rankscolumnpackingmaterialbytheirrelativeabilitiestoretainsolutesonthecolumn
    C.isameasureofthesolventadsorptionenergy
    D.noneoftheabove
    Answer&Explanation
    Answer:OptionA
    11.
    Dwellvolumeisdefinedas
    A.thevolumeofsolventcontainedinaliquidchromatographiccolumn
    B.thetimerequiredforthegradienttoreachthecolumn
    C.thevolumeofthecolumnbetweenthepointatwhichsolventsaremixedandthebeginningofthecolumn
    D.noneofthese
    Answer&Explanation
    Answer:OptionC
    12.
    Voidvolumereferstothe
    A.totalvolumeofeluentinthecolumntheremainderbeingtakenupbythepackingmaterial
    B.thevolumeofsolventcontainedinaliquidchromatographiccolumn
    C.thetimerequiredforthegradienttoreachthecolumn
    D.thevolumeofthecolumnbetweenthepointatwhichsolventsaremixedandthebeginningofthecolumn
    Answer&Explanation

    http://forum.pfmsg.com/threads/biochemistryhplc.3612/

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    MCQsBIOCHEMISTRY:HPLC|PFMSGFORUM
    Answer:OptionA
    13.
    InnormalphaseHPLC,thereisa
    A.nonpolarsolvent/polarcolumn
    B.polarsolvent/nonpolarcolumn
    C.nonpolarsolvent/nonpolarcolumn
    D.anyoftheabove
    Answer&Explanation
    Answer:OptionA
    14.
    Whichofthefollowing(s)is/aretheadvantageofHPLCovertraditionalLPLC(lowpressureliquidchromatography)?
    A.Greatersensitivityandreusablecolumns
    B.Idealforionicspeciesandlargemolecules
    C.Samplerecovery
    D.Alloftheabove
    Answer&Explanation
    Answer:OptionD
    15.
    WhichcanbeusedasamobilephaseinHPLCapplications?
    A.Anycompoundwithsolubilityinliquid
    B.Anycompoundwithlimitedsolubilityinliquid
    C.Anycompoundwithnonsolubilityinliquid
    D.Anyoftheabove
    Answer&Explanation
    Answer:OptionA
    aayisha quddus, Dec 3, 2014

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