Refractometer RFM300plus Man en

RFM300+
Refractometer
User Guide
RFM300+ User Guide (Eng)

Code
Issue
Revision
Date
22-160
02
D
November 2008
Bellingham and Stanley Ltd. has been manufacturing high quality optical instruments in the
UK since 1914 and is a leading manufacturer of refractometers and polarimeters. The current
range of products includes optical and digital hand refractometers as well as a full range of
laboratory refractometers and polarimeters available through a network of trained distributors
throughout the world. Process refractometers are also available through specialist outlets.
Our main website gives full details about Bellingham and Stanley Ltd. and our products.
Foreign language brochures in PDF format may be downloaded from this section of the site
by clicking the flag. We apologise if the foreign language brochure of your choice is not
available.
Contact Sales at Bellingham and Stanley Ltd. to discuss a particular application or to receive
details of your local distributor.
Website:
www.bellinghamandstanley.com
Bellingham + Stanley Ltd. recognise all trademarks
Copyright Bellingham + Stanley Ltd. 2009

Every effort has been made to ensure the accuracy of the contents of the manual. However,
Bellingham + Stanley Ltd. can assume no responsibility for errors contained in the manual or their
consequences.
Printed in UK
Bellingham + Stanley Ltd.

Longfield Road,
Tunbridge Wells, Kent TN2 3EY
United Kingdom
Tel: +44 (0) 1892 500400
Fax: +44 (0) 1892 543115
Bellingham + Stanley Inc.

1000 Hurricane Shoals Road,
Building D, Suite 300,
Lawrenceville, USA GA30043
Tel: 770 822 6898
Fax: 770 822 9165
DECLARATION OF CONFORMITY
According to ISO/IEC 17050-1 & 2 : 2004
Manufacturer's Name
Bellingham & Stanley Limited
Manufacturer's Address
Longfield Road,
Tunbridge Wells,
Kent TN2 3EY
United Kingdom
declares that the product

Product Name
RFM300+ Refractometer
Model Number
All
Is designed to conform to the following Product Specifications:

Safety
BS EN 60950-1:2002
EMC
Emissions
BS EN 55022:2006
BS EN 55022:2006
Radiated Emissions Class A

Conducted Emissions Class A
Immunity
BS EN 61000-3-2:2006
BS EN 61000-3-11:2001
BS EN 61000-4-2:1995
BS EN 61000-4-3:2006
BS EN 61000-4-5:2006
Supplementary
Harmonics
Flicker
ESD 8kV contact
EMS 3V/m
Surges 0.5kV (line to line)
1.0kV (line to earth)
BS EN 61000-4-11:2004 Power outages 1 cycle/100%
The product herewith is designed to comply with the requirements

of the EMC Directive 2004/108/EC and the Low Voltage Directive
2006/95/EC.
This symbol is an internationally agreed indicator that the product bearing it

should not be disposed of as general waste or garbage which might end up in
landfill sites, but should instead be sent for special processing and/or recycling in
those countries where appropriate legislation and facilities are in place.
This symbol indicates a caution or warning, please refer to the manual.
contents
Installing the instrument ............................................. 1
Instrument description................................................ 2
Instrument overview ............................................ 2-1
Basic operation ................................................... 2-4
Display screen information .................................. 2-9
The instrument logs............................................. 2-14
Measurement settings ......................................... 2-15
Measurement settings in detail............................ 2-16
Remote operation................................................ 2-18
Sampling techniques ........................................... 2-19
Use of temperature compensation ...................... 2-20
Air filter replacement ........................................... 2-21
Setting up the system ................................................ 3
Menu flow chart................................................... 3-1
Shortcut keys ...................................................... 3-3
Calibration ........................................................... 3-5
Measurement settings ......................................... 3-8
Methods .............................................................. 3-9
User accessibility ................................................ 3-15
System settings................................................... 3-16
Customising the instrument................................. 3-20
Measurement traceability .................................... 3-22
Adding extra scales............................................. 3-24
Application Corrections ....................................... 3-26
Setting the default values .................................... 3-28
Displaying the software / hardware details .......... 3-28
Specification............................................................... 4
Spares and accessories............................................. 5
Contents
22-16002 Rev. D July 2009
section 1
Installing the instrument
Unpacking the instrument.................................... 1-1
Contents list ........................................................ 1-1
Part numbers....................................................... 1-1
Positioning the system ........................................ 1-2
Mains connection ................................................ 1-2
Power requirements ............................................ 1-2
Power supply adapter ......................................... 1-2
22-16002 Rev. D July 2009
Section 1
Unpacking the instrument

Carefully remove all of the packing material. It is recommended that the box
and other packing materials are retained so that, should the need arise, the
instrument can be safely returned to the manufacturer.
Check that all parts listed below are present and that no transit damage has
occurred. If any are damaged or missing, contact the supplier immediately.
Contents list
1
RFM300+ Refractometer module,

see table below for part number
Power supply, 55-105
Mains lead, see below for part number
Operating Instructions, 22-160
Instruction manual CD-ROM, 55-300
Interconnecting lead (PC serial), 54-07
Spare Air Vent filter
1
1
1
1
1
4
Installation
Part numbers
RFM300+ refractometer part numbers
Model
Complete refractometer
including power supply
Refractometer module
only
RFM330+
22-30
22-330
RFM340+
22-40
22-340
Mains lead part numbers (for use with Power supply 55-105)
Mains cord wire colours
Moulded plug type for
Voltage
Line
(Phase)
Neutral
(Return)
Earth
(Ground)
Code
no.
Switzerland
230V
61-181
Denmark
230V
61-182
India / South Africa
230V
61-188
Australia
230V
61-189
Brown
Blue
Green /
Yellow
61-190
UK 13 Amp square pin

to BS1363/A
230V
61-191
United States (3 pin)
110V
61-192
Europe (Schuko)
230V
61-193
No plug open lead
22-16002 Rev. D July 2009
Page 1-1
Positioning the system

Place the instrument on a flat and stable bench that is:
dry and indoors

away from draughty or hot equipment like fans or heaters
out of direct sunlight or strong ambient light
away from potential sources of interference, such as RFI generating
equipment
within reach of a power point
not using a power circuit that also has large motors or noise generating
equipment connected to it
Mains connection
The power supply adapter is supplied with a moulded mains cord and plug,
to suit one of several socket types. For UK lead, replace fuse only with the
type indicated on the plug.
Installation
Power requirements
Page 1-2
Voltage
Frequency
Maximum current
110 to 230 V~ 10%

50 to 60 Hz
2A
Power supply adapter 55-105

RISK OF ELECTRIC SHOCK:
For electrical safety information, read the label on the power supply
For indoor use only
Must be kept dry
Disconnect the equipment from the mains supply before unplugging the
mains lead from the power supply unit
Do not open the power supply adapter - no user serviceable parts inside
WARNING:
Do not cover, designed to operate with free air convection
No cleaning required
Note: A waterproof power supply adaptor, code no. 55-250, which can be
used in damp environments, is available as an optional extra
22-16002 Rev. D July 2009
section 2
Instrument description
Instrument overview................................................... 2-1
The refractometer................................................ 2-1
The services panel .............................................. 2-2
The display panel ................................................ 2-3
Basic operation .......................................................... 2-4
Switching on........................................................ 2-4
Switching off........................................................ 2-4
Switching on for the first time .............................. 2-4
Initial operation after switching on ....................... 2-5
Manoeuvring through the menus......................... 2-6
Keying in letters, numbers and other characters . 2-7
Keying in numerical values.................................. 2-8
Display screen information......................................... 2-9
Screen title .......................................................... 2-9
Current time ........................................................ 2-9
Softkey functions................................................. 2-9
Temperature control status ................................. 2-10
Taking a reading ................................................. 2-11
Measurement display description........................ 2-12
Reading quality ................................................... 2-12
Printing the readings ........................................... 2-13
24 column print format ........................................ 2-13
Printer options ..................................................... 2-13
The instrument logs ................................................... 2-14
The System log ................................................... 2-14
The Reading log.................................................. 2-14
PC Data Acquisition Program.............................. 2-14
Measurement settings................................................ 2-15
Mode menu ......................................................... 2-15
Measurement settings in detail .................................. 2-16
Scale ................................................................... 2-16
Temp. Comp. (temperature compensation)......... 2-16
Setting the temperature control setpoint ............. 2-17
Stability................................................................ 2-17
Limits................................................................... 2-17
Remote operation ...................................................... 2-18
RS232 serial port ................................................ 2-18
Sampling techniques.................................................. 2-19
Cleaning .............................................................. 2-19
Sample application.............................................. 2-19
Sample presser ................................................... 2-19
Temperature stability........................................... 2-19
Use of temperature compensation ............................. 2-20
Sugar temperature compensation ..................... 2-20
AG Fluid temperature compensation ................. 2-20
Alternative temperature compensations .............. 2-20
Air filter replacement .................................................. 2-21
22-16002 Rev. D July 2009
Section 2
Instrument overview
The refractometer
Presser:
Minimises heat loss/gain.
Excludes strong ambient light from prism.
Minimises sample evaporation.
An alternative presser insert is available depending on
application (page 5-2 and 5-4).
Measuring prism:
Artificial sapphire.
Drip dish:
PEEK plastic
excellent
chemical
resistance and
provides
thermal
isolation.
Prism plate: 316

Stainless steel with
a silicone rubber and
resin prism seal
providing excellent
chemical resistance.
This surface can
become extremely
hot.
Cooling air inlet

vent:
Air passes
through ducting
within the
instrument and
is expelled
through vents at
the rear. This
ensures the
inside of the
instrument is
sealed from the
environment.
These vents
must not be
covered.
Display panel:
see page 2-3.
Housing:
Low density expanded
polyurethane foam. Lightweight
yet good mechanical strength.
22-16002 Rev. D July 2009
Page 2-1
The services panel
Cooling air outlet vents:

Air is drawn in through the side vents and passes through ducting within
the instrument before being expelled through these vents. This ensures
the inside of the instrument is sealed from the environment.
These vents must not be covered.
Code number:
B&S code number
for the instrument
module.
Parallel port:
For printing.
RS232 Serial port 1:
For remote
communication or
printing.
Warning symbol:
See similarly
marked warnings in
this manual.
RS232 Serial port 2:

For connection to
barcode reader.
Disposal symbol:
See Declaration of
Conformity page in
this manual
CE mark:
Specifies the
instrument conforms
to relevant EU
safety and EMI
regulations.
Page 2-2
Serial number:
Always specify this
number in any
communication with
B&S Ltd.
22-16002 Rev. D July 2009
Power connector:
24V dc from
power supply.
The display panel
Temperature
Control
status
indicators:
Operation status bar

Shows the details of the
current operation.
Current time
Power key:
Press to switch ON.
Press for 2 seconds to
switch to STANDBY.
Soft key functions

Soft keys
Functions change
with display.
Power indicator
STANDBY Red.
ON Green.
Arrow keys
Forward/Back keys
for negotiating
through menus etc..
Alpha-numeric keys
Telephone style keys for
entering letters and
numbers
22-16002 Rev. D July 2009
Page 2-3
Basic operation
Switching on
Plug in the power supply and switch on the mains supply. The instrument
will display configuration data and then switch to standby mode with the
power indicator showing red.
Basic operation
To switch on, press the Power Key the Power Indicator will show green, and
the following will be displayed.
Switching off
To switch to standby, press the Power Key and hold down until a
confirmation screen shows (approximately 2 seconds). Press Yes to switch
to standby the Power Indicator will show red, or press No to continue
operating.
Note: It is preferable for the instrument to be left on continuously even if a
reading is to be taken only once per day.
Switching on for the first time

When the instrument is switched on for the first time after delivery, a Setup
Wizard will step you through a number of questions so that the system can
be configured for your particular application.
Initially, it is a requirement to select the language.
Step 1 Setup the standard measurement conditions. These are the
settings which measurements are to be taken, the reading scale and
temperature compensation that should be used. When the correct
conditions are selected, press Quit.
Step 2 Set the clock. Adjust the instrument clock to local time.
Step 3 Set the printer options. Select whether readings are to be
printed, stored in memory or both. When the correct conditions are
selected, press Quit.
The Setup Wizard can be run at any time by selecting the Tools menu.
Page 2-4
22-16002 Rev. D July 2009
Initial operation after switching on
Basic operation
When the instrument is switched on, the temperature control system will be
turned on and the fans will blow air out through the Cooling Air Outlet Vents.
The control system aim temperature will initially be set to 20C. The screen
shown below will be displayed.
Depending upon the ambient temperature it might take up to 10 minutes to

reach 20C.
Once the temperature has
stabilised, a message will be
displayed informing the user
that an Auto Calibration is
required, and to login.
Press OK and the instrument will request that the supervisor PIN is entered.
By default the supervisor PIN is 135, see page 3-15 for more information
about PINs. Logging in is only necessary if PINs are active.
With the PIN entered, the
instrument will request that
the prism is cleaned and that
the presser is lowered.
It is vital that the complete
prism face is scrupulously clean
and dry. Press OK.
A Zero point calibration must then be carried out. The Zero sample value
must be entered. This will initially be 0.00 Brix (distilled water). Press Enter.
Place a small amount of distilled water onto the Measuring Prism. The
complete prism surface must be covered by the water; generally 1ml is
sufficient to achieve this.
Lower the presser.
Press OK.
There will then be a delay while the temperature is stabilising.
When the calibration has been set, the Measurement Display will be
shown.
The refractometer is now ready for use.

It is recommended that the Zero calibration should be repeated daily, see
page 3-5.
22-16002 Rev. D July 2009
Page 2-5
Manoeuvring through the menus

Menu items can be found and selected by either:
Basic operation
pressing the Up/Down softkeys and then pressing Select

or
pressing the item number key i.e. press 3 for Setup
Page 2-6
If the menu item contains a list of options to choose from then either:
select the menu item and then choose the option from the displayed list
or
press the Forward/Back arrow keys to step through the options
Menu items that can be modified by using the arrow keys are identified by
arrows being displayed next to the menu items value (highlighted below).
Return to the Measurement display by repeatedly pressing Quit to step back

through the menus.
22-16002 Rev. D July 2009
Keying in letters, numbers and other characters
Basic operation
When it is necessary to enter in text, such as a method name, the alphanumeric entry screen will be displayed. This will allow entry of numbers,
letters and a range of punctuation characters including ! ? / ( ; etc. by
multiple presses of keys.
E.g. To enter the name

the
the
the
the
the
the
the
the
cola(74) press:
d key 3 times
h key 3 times
g key 3 times
d key 1 time
c key 11 times
i key 5 times
f key 4 times
c key 12 times
c
o
l
a
(
7
4
)
Once a key is pressed, the available characters that can be selected using
that key are shown at the top right of the screen (see the table below).
If a key is not re-pressed within one second, the displayed character will be
accepted.
Key
Available characters
<
>
22-16002 Rev. D July 2009
Page 2-7
Keying in numerical values

Numerical values, such as a refractive index 1.33299 or a polynomial
constant 0.0123456, can entered in either fixed decimal point format or in
scientific notation. E.g.:
Fixed decimal point
1.2345
-123.45
0.00012345
or
Scientific
1.2345E0
-1.2345E2
1.2345E-4
Basic operation
One of the softkeys (second from left) gives access to the minus (-), decimal
point (.) and exponent (E) symbols that will be required to enter both
numerical formats. As the user enters the number, the instrument responds
to the key strokes by changing the soft-key function.
Page 2-8
If an error is made while entering the number, press the CLEAR softkey to
erase the entry.
The ENTER soft-key is available throughout the process and may be pressed
at any time to accept the entry.
Note that if the number being entered is negative, then press the minus softkey before entering the number. Otherwise, if the number is less than 1,
press 0 first to show the decimal point soft-key.
22-16002 Rev. D July 2009
Display screen information

The pictures below describe standard user interface elements.
Basic operation
Screen title
The screen title will describe what is being displayed on the screen.
Current time
The time is displayed in the 24 hour clock. To adjust the time / date see
page 3-16.
Softkey functions
The softkey functions change depending upon what inputs are required at
that time. If a softkey appears grey then that button is disabled.
22-16002 Rev. D July 2009
Page 2-9
Basic operation
Temperature control status
Page 2-10
Thermometer symbol
indicates temperature
control system is
enabled
Aim temperature as
defined in the
measurement settings
The temperature of the sample
The temperature control system has yet to stabilise for the

first time after either switching on or a change to the aim
temperature.
The temperature control loop is stable.
The temperature control system is making an increase

correction.
The temperature control system is making a decrease

correction.
The temperature control loop is stablising.
22-16002 Rev. D July 2009
Taking a reading
Basic operation
Raise the presser to access the prism. The screen will display a message
asking for the presser to be lowered. With the presser raised, the
temperature control is limited, therefore it is beneficial to limit the time the
presser is raised.
Clean the prism thoroughly using a suitable solvent, e.g. water or methyl
alcohol depending on the sample type being measured.
Place a small amount of water or sample on the prism (the circular glass-like
area in the middle of the prism plate). The complete prism surface must be
covered by the sample; generally 1ml is sufficient to achieve this.
Lower the presser, the message to lower the presser will clear.
Press the Read softkey. The instrument will display a progress bar whilst
data is collected and the measurement calculated.
The reading will be displayed using the scale and temperature compensation
set in the Mode menu as described on page 2-16.
The following page describes what each parameter on the measurement display
is.
22-16002 Rev. D July 2009
Page 2-11
Measurement display description

The measurement value is shown
in large figures for easy readability.
It is calculated using the
measurement
settings
shown
below.
Basic operation
The measurement settings include

the measurement scale and
temperature compensation used
(see page 2-15).
Page 2-12
The temperature measured when

the reading was taken is displayed
in degrees Celsius.
The quality measured when the

reading was taken. See below for
details on the quality value.
Reading quality
Quality is an arbitrary figure used to describe the worth of a reading. The
quality value is derived from the optical pattern caused by placing a sample
on the prism. A high quality value indicates a well defined optical pattern
which in turn makes the signal easier to resolve; a low value indicates a less
well defined pattern and hence a less reliable reading.
The quality value for the sample used to set ZERO (normally distilled water)
is automatically set to 100, which can then be used as a reference with which
to compare other measured samples.
22-16002 Rev. D July 2009
Printing the readings

If the fourth (right hand) softkey is set to Print or Prt+Save, the last
displayed reading can be sent to a printer via the selected port. The print
layout can be either with the reading data listed sequentially, suitable for
narrow printers with 24 columns, or with the data in CSV (comma separated
values) suitable for connection to a LIMS. (See Set the print mode and
Available print formats in Section 3)
Basic operation
24 column print format
At the start of the printout is a header. This contains general information

about the instrument, such as when the last span was carried out, and
measurement settings.
Each measurement has a number of parameters. On the left is the
parameter name, and on the right is the parameters value.
Use interconnecting cable 54-03 to connect the instrument to a parallel
printer. (These cables are available from Bellingham + Stanley. Some devices
may require alternatives.)
Printer options
Optionally the reading can be sent to the RS232 serial port for connection to
either a printer or computer terminal program.
22-16002 Rev. D July 2009
Page 2-13
The instrument logs

This instrument has two separate logs for storing data. This data will be held
in the instrument whether the power is on or off and can be downloaded to
a computer using the PC Data Acquisition Program (see below).
The System log
Instrument Logs
The System log maintains a record of all changes and adjustments made to
the instrument by the operators. This will include each zero and span
calibration, changes to the setup, configuration and methods.
Page 2-14
The Reading log

If the fourth (right hand) softkey is set to Save or Prt+Save then pressing it
will save the last displayed reading into the Reading log. Up to 712 readings
can be stored in the log. When the log is full no further results will be able
to be stored, a warning message will be displayed on the screen. The
current status of the Reading log can be viewed by selecting the Saved
results menu option with in the Tools Menu.
The reading log and current status can be
viewed by selecting the Saved results
menu option within the Tools Menu.
Press the Review softkey to display the
contents of the log. When displayed,
pressing the arrow keys will show
additional reading parameters.
PC Data Acquisition Program

The PC Data Acquisition Program (B+S code no. 26-303) enables the data
from both of the above logs to be transferred to a PC at which time the logs
will be deleted in the instrument. The data can then be sorted, easily
viewed and also saved in csv format files, which can then be opened in a
spreadsheet. This program can be downloaded free from the Bellingham +
Stanley website
www.bellinghamandstanley.com
22-16002 Rev. D July 2009
Measurement Settings
The measurement settings are accessed in the Mode menu, which is
available from the Measurement Display.
Mode menu
The mode menu will be familiar to users of other Bellingham + Stanley
instruments.
Reading Mode
The measurement settings are changed from the Mode menu, which is
accessed from the Measurement Display.
Press Quit to return back to the Measurement Display.

The following pages describe each of the measurement settings.
22-16002 Rev. D July 2009
Page 2-15
Measurement settings in detail

This instrument has a number of measurement settings
Scale
Temp. Comp.
Set Temp.
Stability
Limits
(the scale units that the readings are displayed in)

(whether temperature compensation is used)
(temperature the instrument will control the prism to)
(can be set so that a measurement will only be taken if the
sample has stabilised)
(tests to check if the reading parameters are within set
limits)
Scale
Reading Mode
The instrument has a number of scales that measurement can be displayed

in. By default the available scales are
Page 2-16
Scale Name
Refractive Index
Brix
Scale ID
ri
bx
Additional scales can be added, see page 3-24 for details.
Temp. Comp. (temperature compensation)

The instrument has a number of temperature compensation modes
available. By default the available temperature compensation modes are
TC Name
none
sugar
ag fluid
TC ID
no
su
ag
Additional temperature compensation modes can be added, see page 3-25

for details.
22-16002 Rev. D July 2009
Setting the temperature control setpoint

The temperature control system in the instrument can be set over a wide
range of temperatures. For most applications, it is likely that all samples will
be measured at the same temperature; e.g. 20C for a bottling plant or 70C
for a preserves factory. However, some major food manufacturers who
produce a range of different products at one facility may need to measure
different ranges of samples at more than one temperature, e.g. soft drinks at
20C and a range of edible oil products at 60C. To cater for this
requirement, the temperature control setpoint is available as one of the
method parameters.
Reading Mode
However, this must be used with care. It is not practical to continually adjust
the temperature setpoint for every sample. After a change to the setpoint,
the instrument will inhibit reading display until the system has stabilised and a
zero calibration routine has been carried out. Changing the setpoint will
only be practical if, say, all samples to be measured at 20C were carried out
in a morning session and then those at 60C in the afternoon.
The temperature of the stainless steel prism plate will be similar to the
temperature control setpoint and so could become extremely hot. Direct
contact with skin should be avoided when applying sample or cleaning the
prism plate at temperatures above 50C.
Stability
The instrument can automatically detect if the sample has stabilised. There
are number of stability modes from a simple time delay (wait 10 seconds
before taking the measurement) to more sophisticated modes.
This feature is explained in detail from page 3-13.
Limits
The instrument is able to have limits set on a number of parameters. If the

measured sample fails to meet these limits a line will be drawn through the
result.
This feature is explained in more detail on page 3-12.
22-16002 Rev. D July 2009
Page 2-17
Remote operation
Remote operation
Operation of the instrument can be controlled remotely from a computer.

The 54-07 interconnecting cable that is supplied will connect to the COM
port of a typical PC and the instruments RS232 Serial Port 1. Use a
proprietary terminal program or custom software to send commands and
receive data.
Page 2-18
The instrument default RS232 configuration is:

9600 Baud, 8 bit, no parity
Contact Bellingham + Stanley Ltd for a list of serial commands.
When the instrument receives a serial
command, the display will show:
When the action has been completed and the data sent, the instrument will
revert to the normal reading display.
RS232 serial port

The RS232 serial ports can be accessed via the 9 pin D type connectors on
the rear of the instrument. The interconnecting cable socket can be secured
with the 2 screws but these must be finger-tightened only DO NOT OVERTIGHTEN..
9 Pin D type plug connections
Pin No.
2
3
4
5
6
22-16002 Rev. D July 2009
Function
Received data
Transmitted data
DTR
Signal Ground
DSR
Data direction
in
out
In
Out
Sampling techniques
Sampling techniques
In order to achieve the maximum performance from the refractometer, it is
essential that extreme care is taken when cleaning the instrument and
applying sample to the prism. Sample concentration may vary considerably
from the surface to the centre of a mass whether in a beaker or on a spoon
or spatula. Evaporation can cause remarkably rapid drifting unless care is
taken.
Cleaning
Always clean both the prism surface (and the presser if it contacts the
sample) immediately after taking readings. Sample that has remained on the
prism for long periods can be difficult to remove and small particles of dried
matter can subsequently dissolve into later samples.
Use clean tissue with water to remove old sample then dry with fresh clean
tissue. Some samples, particularly oils and other chemicals, will require the
use of a solvent, such as methyl alcohol, for removal and cleaning. NEVER
use acetone, white spirit, 'Genklene', 'Arklone' or any abrasive cleansers on
any painted surface or, particularly, the membrane keypad panel.
Sample application
Always take sample from the centre of a container not from the surface
where higher concentration skin layers will be present. Keep the time taken
to transport the sample from the container to the prism to an absolute
minimum to limit evaporation. With low viscosity liquids, use a disposable
pipette once only. With high viscosity samples, scoop out sufficient with a
clean, dry spatula. Never pour a sample directly from a container onto the
prism.
The temperature of the stainless steel prism plate could become extremely
hot. Direct contact with skin should be avoided when applying sample or
cleaning the prism plate at temperatures above 50C.
Sample presser
The sample presser will improve measurement accuracy and repeatability
by:1. Providing an 'enclosed' environment for the sample so minimising
contact with the air and limiting evaporation.
2. Minimising the effect of ambient air temperature on the controlled
sample.
3. Preventing strong ambient light from affecting the reading quality.
Temperature stability
The refractive index of liquids changes significantly with temperature. It is
important that the temperature of a sample is constant throughout its mass
before an accurate reading can be taken.
22-16002 Rev. D July 2009
Page 2-19
Reading techniques
Use of temperature compensation
Page 2-20
Selecting the temperature compensation mode corrects readings from the

refractometer to a reference temperature, e.g. 20C. That is, it allows the
refractometer to run at the temperature of the product under test but
produces readings as if the system was running at the reference
temperature.
However, temperature compensation should not be considered as just an
alternative to temperature control. If the sample temperature is changing
(e.g. after a warm sample is applied to a cool prism) then there is inevitably a
delay between the actual temperature of the sample in contact with the
prism face and the temperature detected by the temperature probe; (the
probe cannot have instant response). Therefore, there is bound to be some
degree of inaccuracy from the compensation calculations.
Temperature compensation will only be valid if the detected sample
temperature is stable, so there is a great advantage in controlling the prism
temperature to rapidly achieve stability. Using temperature compensation
together with the temperature control system in the instrument could be an
advantage in certain applications. Typically, a hot sample could be taken from
a production line and placed on an instrument controlled at 60C but with
compensation to display the equivalent reading at 20C.
Sugar temperature compensation
sugar temperature compensation will correct readings of water and sucrose

solutions to 20C. It conforms to the published ICUMSA 1978 correction
tables which covers the ranges 10 to 40C and 0 to 80 Brix and has been
extended to cover 5 to 70C by using additional data. Although the
correction is specifically applicable to pure sucrose solutions, it is also valid
for many sugar based food products. However, it must be stressed that the
correction values may be unsuitable for other, non-sugar based, products
and great care should be exercised if sugar temperature compensation is
used with these samples.
AG Fluid temperature compensation
ag fluid temperature compensation will correct readings of AG Fluids to

20C, it covers the range 10 to 40C.
AG Fluids are a part of a range of Calibration Materials available from
Bellingham + Stanley which can be used for verifying and setting the
instrument calibration. The reading variation with temperature of AG Fluids
is different from that of sugar and so ag fluid temperature compensation is
useful when calibrating the instrument at temperatures other than 20C.
Alternative temperature compensations
Alternative temperature compensations can be added for the correction of

sugar solutions to reference temperatures other than 20C or for other
product types. For instructions on how to add custom temperature
compensation modes see page 3-25.
22-16002 Rev. D July 2009
Air filter replacement
Air filter replacement
This instrument is fitted with replaceable filters on the cooling air inlet vents.
The filters will prevent airborne dust or grease entering the ducting and
clogging the fans.
They should be removed and replaced at regular intervals.
The replacement interval could be weekly for environments with high levels
of atmospheric contaminants or up to three monthly for clean room
applications.
Initially, check the filters often to determine how quickly they become
choked.
To replace the filter, pull the filter
cover away from the instrument.
Four spare filters are supplied with
the instrument.
Additional filters are available from
Bellingham+Stanley in packs of 20
(B+S code no. 26-292).
To replace an enhanced
protection filter, unscrew the filter
holder from the instrument body
by turning it in an anti clockwise
direction. Pulling the filter holders
slightly away from the body can
aid their removal.
Once the filter is replaced, simply
screw back on.
Four spare filters are supplied with
the instrument.
Additional filters are available from Bellingham+Stanley in packs of 20
(B+S code no. 22-088).
22-16002 Rev. D July 2009
Page 2-21
section 3
Setting up the system
Menu flow chart.......................................................... 3-1
Shortcut keys ............................................................. 3-3
Calibration.................................................................. 3-5
Calibration standard values................................. 3-5
Zero calibration ................................................... 3-5
Span calibration .................................................. 3-6
Cancel the last calibration ................................... 3-7
Calibration report................................................. 3-7
Measurement settings................................................ 3-8
Which to use, the Mode menu or Methods?........ 3-8
To enable the use of Methods............................. 3-8
To disable the use of Methods (if using the Mode
menu) ................................................................. 3-8
Methods ..................................................................... 3-9
Adding a new method ......................................... 3-9
Editing an existing method .................................. 3-10
Hiding the preset methods .................................. 3-10
Method selection type ......................................... 3-11
Print a list of methods.......................................... 3-11
Reading limits...................................................... 3-12
Adding a limit test condition................................. 3-12
Editing or deleting a limit test condition ............... 3-12
Reading stability.................................................. 3-13
Adding a stability delay condition ........................ 3-13
Adding a stability repeatability condition ............. 3-14
Using smart stability detection............................. 3-14
User accessibility ....................................................... 3-15
Entering PINs ...................................................... 3-15
Changing PINs .................................................... 3-15
System settings.......................................................... 3-16
Setting the date format ........................................ 3-16
Adjust clock for summer (daylight saving) time ... 3-16
Set the clock time................................................ 3-16
Set the print mode............................................... 3-17
Available print formats......................................... 3-17
Select use of ports (serial 1 and parallel) ............ 3-17
Using statistic print mode .................................... 3-18
Automatic printing/saving .................................... 3-19
Set the serial port configuration........................... 3-19
Customising the instrument........................................ 3-20
Change the display layout ................................... 3-20
Change the reading recurrence........................... 3-20
Change the reading resolution ............................ 3-20
Adjust the display backlight and polarity ............. 3-21
Disabling the presser .......................................... 3-21
Section 3
22-16002 Rev. D July 2009
Measurement traceability ........................................... 3-22

Batch codes ........................................................ 3-22
Using barcodes as batch codes .......................... 3-23
User codes .......................................................... 3-23
Adding extra scales.................................................... 3-24
Adding a standard scale...................................... 3-24
Adding a custom designed scale......................... 3-24
Adding a custom designed temperature
compensation...................................................... 3-25
Application Corrections .............................................. 3-26
Offset Correction ................................................. 3-26
Citric Acid Correction .......................................... 3-27
Setting the default values........................................... 3-28
Displaying the software / hardware details................. 3-28
Section 3
22-16002 Rev. D July 2009
Menu flow chart

Menu
1.
2.
3.
4.
5.
Calibration
Tools
Setup
Information
Help
1. Zero
2. Span (Top
Calibration)
3. Undo
4. Report
If Methods are
not enabled
If Methods are
enabled
1.
2.
3.
4.
5.
Methods
Passwords
Display
Reading
System
1. Saved Results
2. Setup Wizard
1.
2.
3.
4.
1. Use Methods
1.
2.
3.
4.
5.
Use Methods
Hide Preset
Selection Type
Print List
normal
zero
span
Add new
Settings
1. Operator PIN .... 123
2. Supervisor PIN . 135
Default settings shown
1.
2.
3.
4.
Header
Temperature
Quality
Configuration
1. no
2. yes
1. single
2. continuous
3. auto single
1.
2.
3.
4.
5.
1.
2.
3.
4.
Recurrance
Resolution
Appl. Correction
Scales
Temp. Comps
1. low
2. medium
1. none
2. acid
3. offset
english
french
spanish
german
1. Add new
1. dd.mm.yy
2. mm.dd.yy
1. Date Format
2. Summer Time
3. Set Clock
1. no
2. yes
1.
2.
3.
4.
1.
2.
3.
4.
5.
Language
Time / Date
Communications
Traceability
Temp. Control
1. Printer
2. Serial Ports
1.
2.
3.
4.
Mode
Serial 1
Parallel
Record After Read
none
5. statistics
printer 6. multi + prt
save
prt + save
1. none
2. 24 column
3. csv (lims)
1. no
2. yes
1. Baud Rate
2. Word Length
1. Batch Codes
2. Auto Increment
3. Log Users
1. Mode
2. Disable presser
22-16002 Rev. D July 2009
1.
2.
3.
4.
5.
off
number
text
dateinc
barcode
1.
2.
3.
4.
4800
9600
19200
38400
1. 7 bit EP
2. 8 bit NP
1. off
2. number
3. text
1. off
2. on
Section 3-1
Page 3-2
22-16002 Rev. D July 2009
Shortcut keys
Shortcut Keys
Shortcut key functions can be activated when the Measurement Display is

shown. Press and hold down the key until the next screen is displayed.
Press and hold to:

switch to
standby
Press and hold to:
adjust the backlight and
display polarity.
22-16002 Rev. D July 2009
Section 3-3
Page 3-4
22-16002 Rev. D July 2009
Calibration
Calibration is essential to ensure accurate readings over the required
measuring range and should be performed at the low end (Zero) and the
high end (Span) of this range with samples of known values.
Zero
This can be considered as an offset to the displayed values.

Although the ideal sample for setting Zero is distilled water,
any known sample at the low end of the measurement
range can be used.
Span
The Span can be considered as a scaling, or multiplying,

factor. Span should be checked with a known sample at the
upper end of the measurement range.
Bellingham + Stanley supply a range of Calibration Materials suitable for

verifying and setting the instrument calibration. See the B+S website,
www.bellinghamandstanley.co.uk .
Calibration standard values
Calibration
The Zero and Span aim values must be entered in the scale units selected in
the measurement settings.
If the instruments measurement settings are being set using Methods, then
either the Zero or Span method will automatically be selected when a Zero
or Span is being carried out.
If the calibration is to be carried out at 20C, then the specified value of the
sample at 20C should be entered.
Alternatively, if the calibration is to be carried out at a temperature other
than 20C and if:
a. the sample is distilled water or a sucrose solution, then sugar
temperature compensation could be selected and the specified value of
the sample at 20C can be entered.
b. the sample is an AG Fluid, then ag fluid temperature compensation
could be selected and the specified value of the sample at 20C can be
entered.
c. the sample is not sucrose or an AG Fluid, then the specified value of the
sample at the calibration temperature must be entered.
Zero calibration
press the Menu softkey
select 1. Calibration
select 1. Zero
A message will be displayed asking for the prism to be thoroughly cleaned
and the presser to be lowered. If a zero has been carried out within the last
30 minutes, the option to skip this part of the calibration is given (this allows
a re-zero to be carried out without removing the sample).
If the clean prism calibration is carried out, it is vital that the complete prism
face is scrupulously clean and dry. Press OK.
22-16002 Rev. D July 2009
Section 3-5
The Zero sample value must then be entered. If distilled water is to used and
the instrument set to 20.0C then the value will be 0.00 Brix or 1.33299
Refractive Index; see Calibration Standard Values above.
The current measurement settings are displayed in the bottom left of the
aim point screen, so that the aim point units can be checked. Press Enter.
Place a small amount of distilled water onto the Measuring Prism. The
complete prism surface must be covered by the water; generally 1ml is
sufficient to achieve this.
Lower the presser.
Press OK.
There will then be a delay while instrument waits for the sample to
stabilise.
When the calibration has been set the instrument will show Calibration
Complete..
Span calibration
Calibration

select 2. Span (Top Calibration)
When requested, enter the Span sample value aim value. See Calibration
Standard Values above.
The current measurement settings are displayed in the bottom left of the
aim point screen, so that the aim point units can be checked. Press Enter.
As with the zero calibration, place the span sample onto the prism and lower
the presser. Press Ok to start the calibration.
Continuous readings will be taken until stable and then the calibration will be
corrected to the new value.
During both Zero and Span calibrations, the spread of the last 5 reading
values is shown in refractive index (irrespective of the scale used for
calibration). This value must be less than 0.00001 for the value to be
accepted.
Page 3-6
22-16002 Rev. D July 2009
Cancel the last calibration

If the Zero or Span calibration was carried out in error, e.g. an incorrect
sample was applied or the wrong value was entered, it can be cancelled.
select 3. Undo
press Yes
Calibration report
Display a report of the last Zero & Span calibrations.
Calibration

select 4. Report
press Print to optionally send the report to a printer which can be

authorised and retained by the operator.
22-16002 Rev. D July 2009
Section 3-7
Measurement settings
The instrument has the following measurement settings that will affect a
reading.
Scale
Temp. Comp.
Set Temp.
Stability
Limits
(the scale units that the readings are displayed in)

(check that the sample has stabilised)
(check if the reading parameters are within set limits)
The measurement settings can be set in one of two ways.

Mode Menu
Measurement
Methods
A Mode menu is accessible from the Measurement

display. The mode menu allows the operator to
select the measurement settings which will be used
for all subsequent readings.
Preconfigured Methods, which define the
measurement settings, are selected from the
Measurement Display.
Which to use, the Mode menu or Methods?

If the complete range of product types that is to be measured on the
instrument requires the same measurement settings, then Mode Menu
option would be most suitable. The mode menu is discussed further on
page 2-15.
However, if a number of products with differing settings are to be read, then
a method can be added for each of them. E.g. a range of soft drinks could be
regularly measured in the sugar scale and the display should show whether
each product reading value is within its set tolerance. The methods can be
given names that are easily identifiable by the operators such as:
Method name
cola
lemon
orange
Low limit
10.9
9.8
11.0
High limit
11.3
10.5
11.4
To enable the use of Methods

select 3. Setup
select 1. Methods
select 1. Use Methods
select 2. Yes
To disable the use of Methods (use the Mode Menu)

select 3. Setup
select 1. Methods
select 4. Settings
(Note: the selection number will change as

methods are added)
select 1. Use Methods

select 1. No
Page 3-8
22-16002 Rev. D July 2009
Methods
A Method contains all the settings necessary for the instrument to take and
check a sample reading. The settings available are:
Scale
Temp. Comp.
Set Temp.
Stability
Limits
(the scale units that the readings is displayed in)

(check that the sample has stabilised)
(check if the reading parameters are within set limits)
Initially, there are three methods set in the instrument:

normal
for measuring samples
zero
the conditions used for a zero calibration
span
the conditions used for a span calibration
The zero and span method only contain Scale and Temp. Comp. settings.
The zero or span calibration procedure will be carried out at the
temperature of the last selected method, i.e. the temperature of the last
measurement reading.
Methods
If the complete range of product types that is to be measured on the

refractometer requires the same measurement settings then the normal
method can be selected and used for all samples. However, if a number of
products with differing settings is to be checked, then a method can be
added for each of them. E.g. a range of soft drinks could be regularly
measured in the Brix scale and the display should show whether each
product reading value is within its set tolerance. The methods can be given
names that are easily identifiable by the operators such as:
Method name
cola
lemon
orange
Low limit
10.9
9.8
11.0
High limit
11.3
10.5
11.4
Adding a new method

select 3. Setup
select 1. Methods
select 4. Add new

methods are added)
Enter the method name. This could be a common name such as cola or
lemonade, a trade name or a product reference code (but not a batch code,
it must identify a generic product type). The name can be a combination of
lower case letters, numbers and other characters.
Set each of the following parameters for the method:
Scale
see page 2-16
Temp. Comp. see page 2-16
Set Temp.
see page 2-17
Stability
see page 3-13
Limits
see page 3-12
then Quit back to the Measurement Display.
22-16002 Rev. D July 2009
Section 3-9
Editing an existing method

select 3. Setup
select 1. Methods
select the method to be edited
To delete a method select option 6, Delete Method.
Hiding the preset methods

The instrument has 3 preset methods; normal, zero and span. If the
refractometer will always be used with the preset methods, i.e. one set of
conditions for all samples, and no other methods have been added, then the
Methods function can be disabled and the second softkey in the
Measurement display will be blank.
Alternatively, if a number of methods are created for a standard range of
products, e.g. cola, orange, lemonade, and the preset methods will never be
required, then it could be advantageous to hide normal, zero and span
from the Methods list. This will make it easier to select the method of
interest.
Methods
To achieve either of these,

select 3. Setup
select 1. Methods
select 4. Settings
Page 3-10
select 2. Hide Preset

select 2. Yes
22-16002 Rev. D July 2009

methods are added)
Method selection type

Before reading a sample, the relevant method should be selected by pressing
the Method softkey in the Measurement Display. This will show a list of all
available methods and the appropriate one can be selected.
However, if a large number of methods have been created, e.g. greater than
20, then it could be easier to select the required method by its index
number rather than scrolling through the list. To change the method
selection method,
select 3. Setup
select 1. Methods
select 4. Settings
select 3. Selection Method
select list or number

methods are added)
List selection type
Number selection type
Methods
Print a list of methods
To print a list of all available methods with their index numbers,

select 3. Setup
select 1. Methods
select 4. Settings
select 4. Print List

methods are added)
22-16002 Rev. D July 2009
Section 3-11
Reading limits
Reading Limits allows up to three test conditions to be set. If any of the
reading parameters values fall outside the limits then the reading is identified
as fail.
The three test conditions can be set to check any, or all, of the available
parameters. These are :
Reading value
Temperature
Quality
Adding a limits test condition

Add a new method or Edit an existing method as detailed above, then
select 5. Limits
select 1. Add new
limits tests are added)
select the parameter 1. reading, 2. temp, 3. quality
enter the Lower Limit
enter the Upper Limit
Methods
Example: A method has been configured with the scale set to Brix and two
limits tests of:
Reading value;
lower limit = 10.5
upper limit = 11.5
Quality;
lower limit = 105
upper limit = 110
Page 3-12
If the Reading value is lower than 10.5 or higher than 11.5

or
the Quality is less than 105 or higher than 110
then the reading will be recorded as fail and displayed with a line
through it:
Editing or deleting a limits test condition

Select an existing method as detailed above, then
select 5. Limits
select the Limits test to be edited
change the parameter or limits as required
or
select 4. Delete
22-16002 Rev. D July 2009
Reading stability
Reading Stability is a condition that must be met before a sample reading can
be displayed. The Stability condition can be set to
none
a simple fixed delay
a number of consecutive readings with a repeatable parameter value.
smart detection, using both reading and temperature measurements to
determine sample stability
A Reading Stability condition will only be functional while Reading
Recurrence is set to Single (see page 3-20).
Adding a stability delay condition

A reading Stability delay condition causes a wait period after the Read
softkey has been pressed before the Read function is actually commenced.
This delay provides a fixed time for the sample to stabilise on the prism and
to achieve thermal equilibrium throughout the sample mass.
The valid delay range is 1 to 9999 seconds.
Methods
From the measurement settings

select 4. Stability
select 2. delay
Enter a delay time in seconds
22-16002 Rev. D July 2009
Section 3-13
Adding a stability repeatability condition

A reading Stability repeatability condition will disable reading values being
displayed until the condition is met.
After the Read softkey has been pressed, the instrument will take readings
until the spread of a number of consecutive parameter values do not exceed
the tolerance set.
The parameter could be the
reading value or reading temperature or quality
Examples: Three different repeatability conditions are shown below. The
values displayed would be after the 3rd, 5th or 6th reading depending on which
condition had been set.
reading
temp
quality
14.70
14.79
14.81
14.83
14.82
14.83
24.84
24.94
24.98
25.02
25.00
24.99
101
104
104
104
103
104
2 quality values within 2

Reading 3 will be displayed
3 reading values within 0.2
4 temp values within 0.05
Methods

select 4. Stability
select 3. repeatability
select the parameter 1. reading, 2. temp, 3. quality
enter the tolerance that the spread of values must be within
enter the number of readings that must be within tolerance.
Note:
The tolerance value entered must be in the units of the selected scale for
the method; e.g. If the scale is Brix then the tolerance could be, say, 0.1 or
if the scale is refractive index then the tolerance could be 0.00015.
The number of readings value must not be greater than 10.
Using smart stability detection

The smart stability option takes the repeatability feature described above
one stage further by testing both the reading and the temperature of the
sample. The combination of both stable reading and temperature will give a
good indication that the sample has stabilised. Measurements are required
to be 0.05 C of the aim control temperature.
select 4. Stability
select 3. smart
enter the tolerance that the spread of values must be within
enter the number of readings that must be within tolerance
Note: The tolerance value entered must be in the units of the selected scale for
the method; e.g. If the scale is Brix then the tolerance could be, say, 0.1
or if the scale is refractive index then the tolerance could be 0.00015.
The number of readings value must not be greater than 10.
Page 3-14
22-16002 Rev. D July 2009
User accessibility
There are 2 PINs in this refractometer; the "operator" PIN and the
"supervisor" PIN. The "operator" PIN allows access to the Mode menu (or
Method selection if enabled) only and the "supervisor" which provides
unrestricted operation of the Mode menu and Setup menu. Each PIN is a 3
digit number in the range 001 to 255 and is initially set as follows:"Operator" 123
"Supervisor" 135
Entering PINs
Accessibility
After pressing SETUP the user is prompted to enter the PIN and press
Enter.
If the PIN is incorrect the instrument will return to the Measurement

Display.
Changing PINs
The "operator" and "supervisor" passwords can be changed to any three digit
numbers in the range 001 to 255.
select 3. Setup
select 5. Passwords
select 1. Operator PIN or 2. Supervisor PIN
Setting the "operator" password to 000 will give unrestricted access to the
Mode menu (or Method selection if enabled) and PIN screen will not be
displayed after pressing Mode.
Setting the "supervisor" password to 000 will give unrestricted access to
both the Mode & Setup menus and the PIN screen will not be displayed after
pressing Mode or Setup.
CAUTION! Access to the MODE & SETUP functions is only possible by
entering the passwords. DO NOT FORGET THEM!
In the event of the PIN being forgotten, contact Bellingham & Stanley Service
Department for assistance.
22-16002 Rev. D July 2009
Section 3-15
System settings
Setting the date format
The instrument can display the date in two formats, dd.mm.yy (typically
UK) and mm.dd.yy (typically US).
select 3. Setup
select 5. System
select 2. Time / Date
select 1. Date Format
Select the required date format from the list.
Adjust clock for summer (daylight saving) time
The instruments clock can easily be adjusted for daylight saving time (adding
one hour to the clock).
select 3. Setup
select 5. System
select 2. Summer Time
Select yes to advance the clock one hour, or no to turn back the clock one
hour.
Settings
Set the clock time
Page 3-16

select 3. Setup
select 5. System
select 3. Set Clock
Enter the time and date digits as shown. The clock will be updated when the
last digit is keyed.
22-16002 Rev. D July 2009
Set the print mode
The fourth (right hand) softkey function in the Measurement Display can be
set to:
Mode
Function
none
print
None; softkey disabled

Print the last displayed reading to the selected
printer port
Save the last displayed reading to the reading log
Print the last displayed reading to the selected
printer port and save it to the reading log
Allows a group of up to 10 measurements to be
recorded and printed with a statistical analysis of the
data (see page 3-18)
Allows a group of between 2 and 10 consecutive
measurements to be read and printed automatically.
save
prt+save
statistics
multi + prt
Softkey
caption
blank
Print
Save
Print
Print
Print
To select the required mode:

select 3. Setup
select 5. System
select 3. Communications
select 1. Printer
select 1. Mode
Available print formats
Settings
This instrument can output data using either the serial port 1 and / or parallel
port. There are 2 formats available.
Format
24 column
csv (lims)
Description
Optimised for use with a 24 column printer.
Designed for easy integration with a LIM system. Each value
is separated using commas.
If a printer is connected to the Parallel port then csv data can also be output
via the Serial 1 port.
Select use of ports (serial 1 and parallel)
If the print mode above is set to printer then the instrument can be
programmed to output data via the serial 1 and parallel port.
select 3. Setup
select 5. System
select 1. Printer
select 2. Serial1 or 3. Parallel
select the desired print format
When configuring the ports it is advisable to first set Serial 1 and Parallel to
none, and then configure the ports.
22-16002 Rev. D July 2009
Section 3-17
Using statistic print mode
The instrument has an optional statistical printout mode (see page 3-17).
This allows a group of up to 10 results to be temporarily logged together and
a statistical analysis carried out upon the results.
When Print is pressed and no previous results have been logged, the
instrument will request the traceability data (batch and operator details) if
selected. This information will be used to identify all of the measurements in
the group.
The diagram below explains the operation of the statistical printout.
Settings
Optional
Page 3-18
Clears the statistics
22-16002 Rev. D July 2009
Automatic printing/saving
The instrument can be configured to automatically record a measurement

following a measurement by enabling the Record After Read option. This
will, depending upon the Mode setting, print or save the result immediately
after a reading.
select 3. Setup
select 5. System
select 1. Printer
select 4. Record After Read
Traceability options, such as batch and operator codes will be requested as if
Print or Save had been pressed.
This feature will only operate in single recurrence mode (see page 3-20).
Set the serial port configuration
Settings
The serial port baud rate and word length can be set to suit the receiving
device:
select 3. Setup
select 5. System
select 2. Serial ports
22-16002 Rev. D July 2009
Section 3-19
Customising the instrument

The instrument can be customised to suit individual operating requirements
both in visual aspects, the layout of the screen & the amount of data
displayed, and operational settings.
Change the display layout

select 3. Setup
select 3. Display
The Header, Temperature, Quality, and Configuration can be individually

selected to be displayed. If all four options are de-selected then the screen
will show only the reading and the softkey functions.
Customisation
Change the reading recurrence
Page 3-20

select 3. Setup
select 4. Reading
select 1. Recurrence
With Single selected, the instrument will only take a reading when the Read
key is pressed and leave it on the display until Read is pressed again.
Continuous mode will disable the Read key and readings will be repeatedly
taken and updated on the screen. A small progress bar will be displayed
about the Read key, to indicate that measurements are being taken.
If a Reading Stability condition has been set (see page 3-13), then this will be
disabled while Reading Recurrence is set to Continuous.
With the auto single mode selected, the instrument will automatically take a
single measurement if a sample is detected after lowering the presser.
Change the reading resolution

select 3. Setup
select 4. Reading
select 2. Resolution
Medium is the normal resolution for the instrument.
Low is one digit lower resolution
22-16002 Rev. D July 2009
Adjust the display backlight and polarity
If the display is difficult to read, especially in either uncommonly bright or

dim ambient lighting conditions, it can be improved by adjusting the backlight
intensity or the display polarity.
The instrument must be in the
Measurement Display screen.
Customisation
Press and hold down the Methods or Mode softkey for approximately
three seconds. The Configure Display screen will show.
Press 1, 2 or 3 for different levels of backlight intensity.

Press 0 to set the display polarity (invert the display).
Press OK to use the new settings or Quit to revert to the previous
condition.
Disabling the presser
Under certain operating conditions it might be necessary to disable the

presser orientation sensor.
To disable the presser
select 3. Setup
select 5. System
select 5. Temp. Control
select 2. Disable Presser
If the presser has been disabled the auto single reading recurrance mode
will not function.
It is advised not to disable the presser unless strictly necessary. Ambient
light not shielded by the presser could cause measurement errors, and the
presser insulates the measurement face from ambient temperature.
22-16002 Rev. D July 2009
Section 3-21
Measurement traceability
All Printed readings are recorded together with the measurement settings
selected and the time & date of the measurement. In addition, an optional
product batch code and a user code can be entered.
Batch codes
If a batch code is required, it can be either a number (in which case the keys
will only enter 0 9) or as text (the keys then having full alpha-numeric
capability).
Setting
off
number
text
dateinc
Traceability
barcode
Page 3-22
Description
The instrument will not request a batch code.
Number from 0 to 32000.
14 character alpha-numeric string.
auto increasing number from 0 to 9999, in the format of
yymmddnnnn
Example
0603117775 (11th March 2006, number 7775)
first 14 characters read from a barcode (see the following
page)
If number is selected, an Auto Increment option is available which will then

offer a batch code which is one greater than the previous used batch code.
select 3. Setup
select 5. System
select 4. Traceability
select 1. Batch codes
If the Auto Increment option is required then
select 2. Auto Increment only available if Batch Codes is set to
number
22-16002 Rev. D July 2009
Using barcodes as batch codes
If a barcode reader is to be used (55-80 or 55-81) it has be to plugged in to

the instruments serial 2 port.
With the instrument showing the
Measurement Display, scan in the barcode.
The display will briefly show Result Printed. or Result Saved. depending
upon printer type configuration.
Traceability
If the printer type is set to printer then the measurement will be printed,
showing the scanned barcode number.
User codes
When the user code option is selected, the user can be recorded in a
number of formats.
select 3. Setup
select 5. System
select 4. Traceability
select 3. Log users
Setting
off
number
text
Description
The instrument will not request a user code.
Number from 0 to 99.
30 character alpha-numeric string.
22-16002 Rev. D July 2009
Section 3-23
Adding extra scales

As supplied, the instrument can display readings in refractive index or Brix
scales. Up to 100 other scales can be added to convert the readings to
standard recognised units, such as Baum, or company specific units
pertaining to a particular product.
Adding a standard scale
The following standard scales are held in a library in memory.
Extra scales
1.
2.
3.
4.
5.
zeiss
oechsle (de)
butyro
42% fructose
55% fructose
6.
7.
8.
9.
0.
90% fructose
baum
sodium chloride
oechsle (ch)
glucose
fructose
invert sugar
probable alcohol
urine solids
kmw (babo)
vol % fsii

select 3. Setup
select 4. Reading
select 4. Scales
select 1. Add new
select 1. From Library
Select the scale required from the list.
The scale will then be available when adding or editing methods.
Adding a custom designed scale
The readings can be converted to custom units using the following formula:
No.
1
2
Equation
reading = A + Bx + Cx2 + Dx3 + Ex4 + Fx5
available later
where:
x = refractive index + Offset

A,B,C,D,E,F are polynomial constants

select 3. Setup
select 4. Reading
select 4. Scales
select 1. Add new

scales are added)
select 2. From Data

Enter the new scale name, (16 characters) e.g. coffee solids
Enter the scale ID, (2 characters) e.g. cs
Enter the 6 constants A to F. Any constants not required, (e.g. E & F with a
4 term polynomial), should be set to 0.
Enter Offset (this can generally be 0 though greater accuracy can be
achieved when calculating a polynomial for a range starting at water by using
an Offset of 1.33)
Enter the Equation no. (e.g. 1, see table above)
Select the Number Format required for displaying and printing the reading.
The scale will then be added to the Scales List and can subsequently be
Altered, Deleted or Copied.
The scale will now be available when adding or editing methods.
Page 3-24
22-16002 Rev. D July 2009
Adding a custom designed temperature compensation
The readings can be temperature compensated by special temperature

coefficients using the following formula:
No.
Equation
Reading Correction = A + Bx + Cx2 + Dx3 + Ex4 + Fx5
available later
where:
x = sample temperature in C
A,B,C,D,E,F are polynomial constants
The Reading Correction is added to the reading as refractive index, before

the reading is converted to Brix or another scale. Therefore, the Reading
Correction must also be calculated in refractive index.
Extra scales
Corrected reading RI = Uncorrected reading RI + Reading Correction

select 3. Setup
select 4. Reading
select 5. Temp. comps
select 1. Add new

temp. comps are added)
Enter the new temp. comp name, (16 characters) e.g. coffee solids
Enter the temp. comp ID, (2 characters) e.g. cs
Enter the 6 constants A to F. Any constants not required, (e.g. E & F with a
4 term polynomial), should be set to 0.
Enter the Equation no. (e.g. 1, see table above)
The temp. comp will then be added to the Temp. comps List and can
subsequently be Altered, Deleted or Copied.
The temp. comp will now be available when adding or editing methods.
Up to 16 temperature compensation modes may be added.
22-16002 Rev. D July 2009
Section 3-25
Application Corrections
This instrument has two special corrections that have been designed for use
in specific applications, offset and acid correction.
Appl. Corrections
The application corrections are selected by

select 3. Setup
select 4. Reading
select 3. Appl. Correction
Select the application correction required from the list.
Page 3-26
Offset Correction
When selected as the application correction (see above) the offset

correction allows the user to easily change the reading value by adding a
simple offset and scaling factor to the measurement. This can be used when
it is required to adjust a refractometer to read that of a density meter.
y = (x + O) S
Where
y = corrected reading in user units

x = reading in user units
O = offset
S = scaler (scaling factor)
The offset and scaling factor are part of the instruments measurement
settings, so if Methods are used each method can have its own parameters
and if Methods are not used then they are set globally from the Mode menu.
22-16002 Rev. D July 2009
Citric Acid Correction
When selected as the application correction (see page 3-26) the acid
correction allows automatic correction of the effects of citric acid on the
measurement. The correction conforms to BS EN 12143:1996.
Appl. Corrections
The percent acid value (total acid expressed as anhydrous citric acid) is part
of the instruments measurement settings, so if Methods are used each
method can have its own default acid value, and if Methods are not used
then they are set globally from the Mode menu.
The acid correction is only active when the Brix scale is selected.
Also be aware that the acid correction is used when calibrating the
instrument, so please ensure that the percent acid value is set to 0 when
calibrating. If Methods are used the zero and span methods automatically set
the acid value to 0.
When a measurement is printed or saved the operator is given the
oportunity to change the percent acid value.
22-16002 Rev. D July 2009
Section 3-27
Setting the default values

If the instrument fails to start up correctly after switching on, or it is
functioning unexpectedly, it could be advisable to reset certain settings to
the original factory set (or default) values.
Default values
Unplug the instrument. Press, and hold down, the far left soft key (power)
and plug the instrument back in. Do not release the key until LED lights and
then goes out. When the key is released the screen will show
Initially the two options will be set to no and none. Select the settings that
you want to return to default values by setting them to show yes. The
Setup option has further options within it. When set correctly press
Quit at the Default Menu.
The instrument will ask the user to confirm their choice. Selecting Yes will
carry out the default.
Displaying the software / hardware details

If contacting B+S, or a local distributor regarding your instrument it is
advised to have the software and hardware details to hand. This information
can be viewed from the Information Menu.
select 4. Information
select 1. Software or 2. Hardware
Example software details
Page 3-28
22-16002 Rev. D July 2009
Example hardware details
section 4
Specification
Performance........................................................ 4-1
Temperature........................................................ 4-1
Temperature compensation ................................ 4-1
Prism ................................................................... 4-1
Prism plate .......................................................... 4-1
RS232 configuration............................................ 4-1
Physical............................................................... 4-2
Power requirements ............................................ 4-2
Specification
22-16002 Rev. D July 2009
Section 4
Specification
Performance
Model
RFM330+
RFM340+
Refractive index
Range minimum
1.32
Range maximum
1.58
Display resolution
0.0001
Accuracy ()
0.0001
0.0001 /
0.00001
0.00004
Specification
Brix
Range minimum
Range maximum
100
Display resolution
0.1
0.1 / 0.01
Accuracy ()
0.1
0.03
Temperature
Ambient operating range

5 to 45C
Measuring low limit
0 or 10 C below ambient (whichever greater)
Measuring upper limit
70C
0.05C
Stability
0.03C
Sensor Accuracy
Storage
-5 to 60C
Temperature compensation
Sugar (ICUMSA)
Sugar (extended)
AG fluid
Prism
Material
ND
Sample surface diameter
Prism plate
Material
Dish diameter
Prism seal
Spill barrier material
RS232 configuration
Baud rate
Word length
Default settings underlined.
Specification
22-16002 Rev. D July 2009
10 to 40C
5 to 10 and 40 to 70C
10 to 40C
Artificial sapphire
1.7681
12 mm
316 stainless steel

59 mm
Silicone rubber and resin
PEEK
4800, 9600, 19200, 38400

7 bit even parity or 8 bit no parity
Page 4-1
Physical
Length (refractometer module only)

Width (refractometer module only)
Height (refractometer module only)
Weight (including power supply)
Power requirements
Voltage
Frequency
Maximum current
335
210
160
5.5
mm
mm
mm
Kg
110 to 230 V~ 10%

50 to 60 Hz
2A
Specification
The RFM 390+ model is a RFM300+ instrument designed to a customers specific

requirements. Therefore the instruments specification may differ from those
shown above. For further information please contact Bellingham + Stanley.
Page 4-2
22-16002 Rev. D July 2009
section 5
Spares and accessories
Calibration AG Fluids .......................................... 5-1
Calibration Oils.................................................... 5-1
Printers................................................................ 5-1
Barcode readers.................................................. 5-1
Interconnecting cables ........................................ 5-1
Instruction manuals ............................................. 5-2
Power supplies.................................................... 5-2
Spares................................................................. 5-2
Enhanced Protection Pack .................................. 5-3
Sample contact presser insert............................. 5-4
Switch membrane anti-splash cover ................... 5-4
Waterproof Power Supply ................................... 5-4
22-16002 Rev. D July 2009
Section 5

Calibration AG Fluids
AG Fluids are an organic solution calibrated in Brix and RI, and have a long
shelf life. They have no special storage or transit requirements and are
therefore easy to ship and store.
AG Fluids are manufactured on a %weight/weight basis in a UKAS
laboratory using only the highest quality chemicals and Analar distilled
water. Manufactured in large batches, the resultant samples are checked
using a controlled refractometer that has been carefully calibrated with NIST
and PTB primary standards, providing further traceability.
Type
Accessories
AG2.5
AG5
AG7.5
AG10
AG11.2
AG12
AG12.5
AG15
AG40
Specification
Refractive
Index*
1.33659
1.34026
1.34401
1.34782
1.34968
1.35093
1.35171
1.35568
1.39986
Brix**
2.50
5.00
7.50
10.00
11.20
12.00
12.50
15.00
40.00
Single 5ml
Bottle
90-401
90-402
90-403
90-404
90-405
90-406
90-407
90-408
90-418
Multi-pack of 20
x 5ml Bottles
90-601
90-602
90-603
90-604
90-605
90-606
90-607
90-608
90-618
* Refractive index @ 589.3nm & 20.0C

** Equivalent Brix value
Calibration Oils
Calibration Oils are one of the most commonly used materials for calibrating
refractometers as they have good traceability, particularly to NIST.
Description
Calibration Oil
Calibration Oil
Specification
Refractive Index*
Brix**
1.46737
70.78
1.51822
90.17
Code
Multi-pack of 5 x 5ml Bottles
90-525
90-530
* Typical Refractive Index @ 589.3nm & 20.0C

**Equivalent Brix value @ 589.3nm & 20.0C.
Printers
Code
55-11
55-12
55-13
55-14
55-15
55-16
Barcode readers
Code
55-80
55-81
Interconnecting cables
Code
54-02
54-03
54-07
Dot matrix impact printer parallel: Euro version 230V

Dot matrix impact printer parallel: UK version 230V
Dot matrix impact printer parallel: US version 110V
Dot matrix impact printer serial: Euro version 230V
Dot matrix impact printer serial: UK version 230V
Dot matrix impact printer serial: US version 110V
See Spares below for printer spare parts
230V version
110V version
Printer serial (25way D type)

Printer parallel (25way D type to Centronics)
Computer (9way D type)
Accessories
Code
Multi-pack of
5 x 5ml Bottle
90-501
90-502
90-503
90-504
90-505
90-506
90-507
90-508
90-518
22-16002 Rev. D July 2009
Page 5-1
Instruction manuals
English
French
Spanish
German
Printed copy & PDF format file

PDF format file
PDF format file
PDF format file
Power supplies
Power supply (unsealed) 110-230V

Mains lead for 55-105 with plug suitable for:
United Kingdom
Euro (Schuko)
United States
Switzerland
Denmark
India / South Africa
Australia
No plug open lead
Power supply (waterproof): 110-230V without mains plug
Accessories
Spares
Page 5-2
Sample contact presser insert

Air filter pack (standard filter)
Quantity: 20
(enhanced protection filter)
Quantity: 20
Paper for dot matrix impact printers
Quantity: 20
Printer ribbon for dot matrix impact printers
Switch membrane anti-splash cover
Enhanced protection pack
Validation documentation (IQ OQ PQ)
22-16002 Rev. D July 2009
Code
22-160
22-161
22-162
22-163
Code
55-105
61-191
61-193
61-192
61-181
61-182
61-188
61-189
61-190
55-250
Code
22-017
26-292
22-088
55-91
55-93
26-155
22-80
22-451
Accessories
Enhanced Protection Pack - Code 22-80

The Enhanced Protection Pack comprises improved air filter holders, filters
and an outlet vent cover. The pack is designed to improve protection of the
instruments internal thermoelectrical temperature control components.
In certain harsh environments the standard air filters and cooling air outlet
vents could become covered with sample, and over time this could block the
airflow, which would affect the temperature control abilities of the
instrument.
The Enhanced Protection Pack air filters have a greater filtering ability and
are more tolerant to airbourne contaminants, which will result in longer
periods between airfilter changes. The outlet vent cover stops the outlet
vents from becoming blocked, if sample should run down the back of the
instrument.
Accessories
To fit the Enhanced Protection Pack simply remove the standard side air
filter holders (as if changing the filter). Replace the standard filter holders
with the EPP air filter holders. Fit the outlet vent cover by pressing the
cover into the outlet vent cover flange.
Accessories
Please note that as the EPP is attached to the instrument by using

push fittings, the instrument cannot be carried by any part of the
EPP.
See page 2-21 for details on changing the EPP air filter.
22-16002 Rev. D July 2009
Page 5-3
Sample contact presser insert - Code 22-017

If the instrument is to be used with samples that are very viscose or solid the
standard (non-contact) presser can be replaced with a sample contact
presser insert. This insert causes a sample to be pressed against the prism
when the presser is lowered.
As the presser comes into contact with sample, it must be
thoroughly cleaned between sample applications to stop cross
contamination of samples.
To fit, unscrew the four screws on the
top of the presser arm. The presser
insert and arm can now be separated.
Fit the new presser insert, and replace
the four screws to hold the insert in
place.
Accessories
Switch membrane anti-splash cover Code 26-155

Splash Covers may be fitted over
instrument switch panels to prevent
contamination by operators when
using the equipment.
The covers help prevent moisture
ingress and provide extra protection
where sample or solvent may react
with the switch membrane.
The splash covers fix to the front of the instrument by adhesive strips and
are made from Polyether Polyurethane.
Waterproof Power Supply - Code 55-250

A Waterproof Power Supply can be used in place of the standard
Bellingham+Stanley Ltd. non-sealed unit if it is to be operated in a wet or
humid environment.
It is supplied with an open-ended cable allowing the fitment of a suitably
waterproof plug in accordance with local regulation and should be installed
by a qualified person.
Specification
Ambient Temperature
Physical L x W x H
Rating
Voltage
Frequency
Page 5-4
5 to 40C
25 x 8 x 6 cm
IP 66
100 - 240v~ 10%
50 - 60 Hz
22-16002 Rev. D July 2009
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RFM300+

RFM300+ User Guide (Eng)

Bellingham + Stanley Ltd. recognise all trademarks

Copyright Bellingham + Stanley Ltd. 2009

Bellingham + Stanley Ltd.

Bellingham + Stanley Inc.

Bellingham & Stanley Limited

declares that the product

Is designed to conform to the following Product Specifications:

Radiated Emissions Class A

The product herewith is designed to comply with the requirements

This symbol is an internationally agreed indicator that the product bearing it

22-16002 Rev. D July 2009

Installing the instrument

22-16002 Rev. D July 2009

Unpacking the instrument

RFM300+ Refractometer module,

India / South Africa

UK 13 Amp square pin

United States (3 pin)

No plug open lead

Installing the instrument

22-16002 Rev. D July 2009

Positioning the system

dry and indoors

110 to 230 V~ 10%

Power supply adapter 55-105

22-16002 Rev. D July 2009

Installing the instrument

22-16002 Rev. D July 2009

Prism plate: 316

Cooling air inlet

22-16002 Rev. D July 2009

The services panel

Cooling air outlet vents:

RS232 Serial port 2:

22-16002 Rev. D July 2009

The display panel

Operation status bar

Soft key functions

22-16002 Rev. D July 2009

Switching on for the first time

22-16002 Rev. D July 2009

Initial operation after switching on

Depending upon the ambient temperature it might take up to 10 minutes to

The refractometer is now ready for use.

22-16002 Rev. D July 2009

Manoeuvring through the menus

pressing the Up/Down softkeys and then pressing Select

Return to the Measurement display by repeatedly pressing Quit to step back

22-16002 Rev. D July 2009

Keying in letters, numbers and other characters

E.g. To enter the name

22-16002 Rev. D July 2009

Keying in numerical values

22-16002 Rev. D July 2009

Display screen information

22-16002 Rev. D July 2009

Temperature control status