Cobas 4u11 Host Interface Manual New Upload

Roche Diagnostics International Ltd
cobas u 411
Host Interface Manual
Version 2.3
Version 2.3 1 / 54
cobas u 411 Host Interface Manual
DISCLAIMER
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SPECIFICALLY DISCLAIMS ANY IMPLIED WARRANTIES, INCLUDING THE IMPLIED
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COPYRIGHT
COPYRIGHT 2011, BY ROCHE DIAGNOSTICS INTERNATIONAL LTD. ALL RIGHTS

RESERVED. NO PART OF THIS PUBLICATION MAY BE REPRODUCED WITHOUT THE
EXPRESS WRITTEN PERMISSION OF ROCHE DIAGNOSTICS INTERNATIONAL LTD.
VERSION HISTORY
Version Date Software version Modifications

1.0 Mar-2007 3.0 -First Release
1.1 Oct-2008 3.0.4.0801 -Implementation of US ranges
-Interface chapter 3 revised
-Further Help chapter 1.4 revised General bug fixing
2.0 Apr-2010 3.1.0.0901 -Implementation of Reference ranges
-Message Header Record chapter revised
-General wording corrections
2.1 May-2011 3.2.0.1001 -General wording corrections
-Chapter Introduction and Purpose rewritten
-Chapter Supported Host Protocols moved
-Chapter Size of Sample ID moved
-Chapter Flags created
-Chapter Transmitted Date moved and rewritten
-Chapter Host Interface Configuration rewritten
-Chapter Error Handling rewritten
-Chapter Sediment Result Record removed
-Result Record no. 9 modified
-All examples updated
-Reference ranges removed
2.2 Sep-2014 3.2.0.1001 Link to internal document removed
new email-address added
2.3 Jun-2015 3.2.1.1302 No changes / modifications
3.2.2.1409
3.3.0.1314
3.3.1.1502
Version 2.3 2 / 54
1 Purpose ....................................................................................................................................... 4
1.1 Document structure .............................................................................................................. 4
1.2 Audience ............................................................................................................................... 4
1.3 Related documents ............................................................................................................... 4
1.4 Used syntax and abbreviations............................................................................................. 5
1.5 Support ................................................................................................................................. 5
2 Introduction ................................................................................................................................ 6
2.1 Overview cobas u 411 analyzer............................................................................................ 6
2.2 Supported host protocols ...................................................................................................... 6
2.3 Sample ID length .................................................................................................................. 6
2.4 Flags ..................................................................................................................................... 6
3 Transmitted Data ........................................................................................................................ 8
3.1 Upload (cobas u 411 to host system) ................................................................................... 8
3.2 Arbitrary units........................................................................................................................ 9
3.3 Download data (host system to cobas u 411)..................................................................... 10
4 Host interface configuration ................................................................................................... 11
4.1 Communication settings ..................................................................................................... 11
4.2 Host interface behaviour ..................................................................................................... 11
4.3 Serial cable ......................................................................................................................... 12
5 Error handling (behaviour of a failed transmission) ............................................................. 13
6 Description of the supported protocols ................................................................................. 14
6.1 ASTM plus protocol ............................................................................................................ 14
6.1.1 General ....................................................................................................................... 14
6.1.2 Communication startup ............................................................................................... 15
6.1.3 Record description ...................................................................................................... 16
6.1.4 ASTM plus trace file examples.................................................................................... 22
6.2 ASTM Urisys 2400 protocol ................................................................................................ 37
6.2.1 General ....................................................................................................................... 37
6.2.2 Communication structure ............................................................................................ 39
6.2.3 Record descriptions .................................................................................................... 39
6.2.4 ASTM Urisys 2400 trace file examples ....................................................................... 46
7 Appendices ............................................................................................................................... 51
7.1 Appendix 1: ASCII-table ..................................................................................................... 51
7.2 Appendix 2: Definition of test codes and test numbers (for ASTM plus protocol
only) .......................................................................................................................................... 52
7.3 Appendix 3: Printouts of control and sample results........................................................... 53
Version 2.3 3 / 54
Purpose
1 Purpose
The purpose of this document is to describe the host interface specifications of the cobas u 411
analyzer. These specifications define the hardware and software settings and the data interchange
via null modem cable (RS-232) between the cobas u 411 and the host system.
1.1 Document structure
This document is structured in a general part for all supported host protocols (chapter 2 to 5) and a
protocol specific part (chapter 6).
The general part contains information about host interface configuration, cable wiring, brief
overview about the different supported host protocols, description of flags and error handling.
The protocol specific part contains a sub-chapter for each supported host protocol, in which the
communication structure and the records are described. There is a detailed example for a
better comprehension, at the end of each record description. The whole trace files of different
data interchanges are added at the end of the corresponding protocol description.
There are additional information in the chapter appendices, such as ASCII-Table, Definition of Test
Codes and Test Numbers for ASTM plus and the printouts of the different Control and Sample
Results used in the examples.
1.2 Audience
This document is written for engineers who must configure the cobas u 411 host interface in the
environment of a laboratory. Depending on the type of host system and on the work flow in the
laboratory the cobas u 411 host interface offers a set of configurable features.
1.3 Related documents
Related documents:
[1] E 1394-91 standard specification for transferring information between clinical
instruments and computer systems, American Society for Testing and Materials
(ASTM)
Version 2.3 4 / 54
Purpose
1.4 Used syntax and abbreviations
Used Syntax
<SpecimenID> Meaning any string (not containing delimiters), representing a
value of the ASTM field SpecimenID.
char Single character. Content specified by standard.
text String. Variable length.
pos_int Positive integer (0 to 65535)
d_t Date and time format as specified by ASTM
(YYYYMMDDHHMMSS)
date Date format as specified by ASTM (YYYYMMDD)
Terms
ASTM American Society for Testing and Materials
LIS Laboratory Information System (host system)
1.5 Support
In case of questions please contact your local Roche Diagnostics Service Department, or
alternatively the Global Customer Support:
E-Mail address: global.gcs_rpd-urin@roche.com
Clarify queue: GSSCDIRIC
Version 2.3 5 / 54
Introduction
2 Introduction
The purpose of this document is to describe the host interface specifications of the cobas u 411
analyzer. These specifications define the hardware and software settings and the data interchange
via null modem cable (RS-232) between the cobas u 411 and the host system.
2.1 Overview cobas u 411 analyzer
The cobas u 411 analyzer is a semi-automatic urinalysis system intended for in vitro qualitative or
semi-quantitative determination of urine analytes, including specific gravity (SG), pH, leukocytes,
nitrite, protein, glucose, ketones, urobilinogen, bilirubin, erythrocytes and color.
2.2 Supported host protocols
The cobas u 411 analyzer supports the following host protocols:

ASTM plus
ASTM Urisys 2400
2.3 Sample ID length
The cobas u 411 analyzer supports a sample ID with a maximum length of 13 characters.
2.4 Flags
The result data which is transmitted to the host system (or printed) must be checked for flags.
There can be different flags, which are sent in a Comment Record additionally to the Result
Record or in the Result Record itself, using the ASTM plus or ASTM Urisys 2400 protocols. On the
printout, the flags are next to the data. The meaning of the flags is listed below.
Attention: Flags are in order to bring some of the values to attention of the operator. Therefore
it is important that these flags are displayed on the host system. If these flags are
not displayed, this could mislead the operator to wrong conclusions and medical
decisions!
Result flags
Flag Result flags Explanation
S Sieve Test parameter result corresponds to sieve criteria settings
* Abnormal For patient sample test parameter result corresponds to
abnormal criteria settings
# Changed reflectance Initial range table is changed
value
! Edited result Data has been edited
Version 2.3 6 / 54
Introduction
Sample flags
Flag Sample flags Explanation
T Test strip Error during measurement of test strip or reference strip.
Possible reasons:
Strip upside down
Strip incompletely wet
Strip completely dry
Strip missing or wrong positioning
Calculation not possible
C Calibration exceeded Calibration interval of four weeks is exceeded
Control flags
Flag Control flags Explanation
S Sieve Test parameter result corresponds to sieve criteria settings
* Abnormal For control sample it means out of range
T Test strip Error during measurement of test strip or a reference strip.
Possible reasons:
Strip upside down
C Calibration exceeded Calibration interval of four weeks is exceeded
Additional flags for ASTM Urisys 2400 protocol only

Flag Sample flags Explanation
R Test strip error Error during measurement of test strip or reference strip.
Possible reasons:
Strip upside down
X Order cannot be done Usually in combination with the R flag
Version 2.3 7 / 54
Transmitted Data
3 Transmitted Data
The cobas u 411 analyzer always initiates the communication to the host system. Hence, the
cobas u 411 analyzer is the master and the host system the slave.
3.1 Upload (cobas u 411 to host system)
Depending on the selected host protocol, different data is sent to the host system. The tables
below give an overview about the different protocols and their transmitted data.
ASTM Urisys 2400
ASTM Urisys 2400

ASTM plus
ASTM plus
Protocol
Protocol
Data Data
Sample ID / Sequence number Name and lot ID

Date and time of calculation Control results for three levels
Strip results Date and time of calculation
Units Units
Flags Flags
Color and clarity of sample Color
Strip Result
Sediment results - compensated raw data (optional)

Control Result
compensated raw data (optional) Instrument ID / serial no.

Instrument ID / serial no. User ID
User ID Software version
Software version Range table (boundary setting) -
Range table (boundary setting) - Test strip lot number -
Test strip lot number - Test strip expiry date -
Test strip expiry date - Calibration strip lot number -
Calibration strip lot number - Calibration strip expiry date -
Calibration strip expiry date - Control lot number
Control name
Control expiry date -
Legend: Supported - not supported
Note: There are two different names for the same type of protocol, depending on which
analyzer is in use:
Using the Urisys 1800 analyzer, the protocol is called "Urisys 2400"
Using the cobas u 411 analyzer, the protocol is called "ASTM Urisys 2400".
Within this document the name ASTM Urisys 2400 for the cobas u 411 analyzer is
used.
Version 2.3 8 / 54
Transmitted Data
ASTM plus protocol

Strip Results Control Results
Strip results Date and time of control calculation
Units Units
Flags Flags
Sediment results Compensated raw data (optional)
Compensated raw data (optional) Instrument ID
Instrument ID User ID
User ID Software version
Software version Range tables (boundary setting)
Range tables (boundary setting) Test strip lot number
Test strip lot number Test strip expiry date
Test strip expiry date Calibration strip lot number
Calibration strip lot number Calibration strip expiry date
Calibration strip expiry date Control lot number
Control name
Control expiry date
ASTM Urisys 2400

Strip Results Control Results
Strip results Date and time of control calculation
Units Units
Flags Flags
Compensated raw data (optional) Compensated raw data (optional)
Instrument ID Instrument ID
User ID User ID
Software version Software version
Control lot number
Control name
3.2 Arbitrary units
ASTM plus
Arbitrary units are sent to the host system in combination with other units (Conventional or SI).
With the control results, the preset arbitrary values (default) are transmitted.
ASTM Urisys 2400

Arbitrary units are sent to the host system only when they are selected separately and not in
combination with other units (Conventional or SI).
With the control results, the preset arbitrary values (default) are transmitted.
Version 2.3 9 / 54
Transmitted Data
3.3 Download data (host system to cobas u 411)
The data download from the host system to the analyzer is performed on the cobas u 411
analyzer by clicking the <Download> button. The following data can be transmitted from the host
system to the cobas u 411 analyzer: Worklist (list of sample IDs)
Note: The ASTM Urisys 2400 protocol does not support this mode.
Version 2.3 10 / 54
Host interface configuration
4 Host interface configuration
The communication between the cobas u 411 analyzer and the host system is established using a
serial asynchronous interface cable. The communication settings must be equal on both sides
(cobas u 411 analyzer and host system), see chapter 4.1 below.
4.1 Communication settings
The host settings on the cobas u 411 analyzer are set to OFF by default. To establish the host
communication, the host settings must be set to ON. Changes in the cobas u 411 analyzer
communication parameters are only possible if the host settings are set to OFF. To configure the
host settings and to enable the host communication, go to the configuration menu: Utilities >
System Parameters > Host Comm.
The following host communication settings are configurable in the cobas u 411 analyzer user
interface.
Configuration Specification Default

Baud rate 1200, 2400, 4800, 9600, 19200, 38400, 57600 9600
Data bits 7, 8 8
Stop bits 1, 2 1
Flow control None, Xon-Xoff, RTS-CTS None
Parity None, odd, even None
4.2 Host interface behaviour
The host interface behaviour can be influenced by the following configuration setup (user
interface):
Item Specification Default

Host ON, OFF OFF
Protocol ASTM plus, ASTM Urisys 2400 ASTM plus
Raw data to host OFF, ON, upload only (setup in service) OFF
Checksum ON, OFF ON
The automatic result transmission is active when the host support is switched to ON.
Version 2.3 11 / 54
Host interface configuration
4.3 Serial cable
The cobas u 411 analyzer uses a serial asynchronous interface cable (RS 232) connected to the
host system. The port COM 2 of the host system is usually allocated to this communication, but
can vary depending on the host system.
According to the serial asynchronous specification, the maximum cable length is 15 meter. It is
recommended by Roche to use a 3 meter cable. All other cable length apart from the
recommended must be organized locally.
The following cable is assigned to use for connecting the cobas u 411 analyzer to the host system:
Host Interface Cable RS 232, IBM PC Null modem D-Sub 9 pins, 3m
Please see table below for wiring details:
cobas u 411 Host System

Relevance
Plug 9 pin female Plug 9 pin female
1 - Legend:
2 RXD 3 RxD = Receiving data
3 TXD 2 TxD = Transmitting data
4 DTR 6 DTR = Data terminal ready
5 GND 5 GND = Ground
6 DSR 4 DSR = Data set ready
7 RTS 8 RTS = Request to send
8 CTS 7 CTS = Clear to send
9 -
Note: If flow control is set to None or Xon-Xoff then the pin 4/6 and pin 7/8 (handshake
connections) are not required.
Version 2.3 12 / 54
Error handling (behaviour of a failed transmission)
5 Error handling (behaviour of a failed transmission)
There are two different types of error handling:

Host system does not acknowledge the transmission:
An alarm message (red alarm LED is activated) will occur if the transmission of data is not
confirmed by the host system. The failed transmission will not be repeated.
Host receives wrong data:

In case the host system has received wrong data (e.g. wrong checksum), the incorrect
sequence will be repeated. After three failed attempts, the transmission will be aborted and
an alarm message will be displayed (red alarm LED is activated).
Version 2.3 13 / 54
Description of the supported protocols - ASTM plus protocol
6 Description of the supported protocols
6.1 ASTM plus protocol
6.1.1 General
This chapter describes the ASTM plus protocol. The ASTM plus protocol consists of Records,
Frames and Messages.
A record is the smallest logical group of information
A frame is a defined data structure that contains a data block to be transmitted
Messages are a collection of several records packed in frames into a logical unit
Note: The ASTM plus protocol is based on the ASTM protocol specification.
Records
A record is the smallest logical group of information. The ASTM plus protocol supports the
following records:
Identification Name Description

H Header record First record in every message, mandatory
P Patient information This record is used to download patient demographics
record from the host system to the analyzer. With the cobas u
411 analyzer, no patient data can be handled.
Therefore the patient record is always empty.
O Order record This record defines the attributes of a particular order
R Result record Measurement data
C Comment record This record is used for the transmission of sample flags
and result flags.
M Manufacturer record This record is used for the transmission of raw data,
additional information for patient and control results
Q Request information Worklist request (query)
record (query)
L Termination record Last record in every message, mandatory
Frames
A frame is a defined data structure that encapsulates a data block to be transmitted. According to
the ASTM plus protocol specification, a frame may contain one or more records. However, the
implementation in the cobas u 411 analyzer contains one record only. Each frame has the
following structure:
Description Start of Frame Protocol End of Check- Carriage Line feed

text no. specific text sum return
ASCII STX record ETX CR LF
(hex) (0x02) data (0x03) (0x0D) (0x0A)
Version 2.3 14 / 54
The frame no. always starts at 1 and will be incremented by one for each record. If the frame
reaches no. 7, the numbering starts at 0 (zero).
Messages
Messages are collections of several records packed in frames into a logical unit. A message starts
with the Header Record (H) and finishes with the Termination Record (L).
6.1.2 Communication startup
Only the cobas u 411 analyzer can start a communication. The cobas u 411 analyzer is always
the master, the host system the slave.
Exception: After the successful handling of the worklist request sequence, the master function
temporarily belongs to the host system for the duration of the worklist transmission.
A communication is initialized when the ASCII character ENQ

(Hex 05) is sent to the host system.
The host system (slave) acknowledges its readiness to

communicate by sending the ASCII character ACK (Hex 06) or
if it is not ready the ASCII character NAK (Hex 15).
If the host system has positively acknowledged its readiness to

communicate (ACK), the cobas u 411 analyzer transmits the
message frame by frame.
cobas u 411
The host system confirms the frame reception with ACK.
The host system can trigger a resending of the last sent frame
with NAK.
The communication session is terminated with the ASCII

character EOT (Hex 04).
Version 2.3 15 / 54
6.1.3 Record description
In this chapter, the meaning of the fields in individual records will be explained in the form of tables.
Fields which are not supported by the cobas u 411 analyzer are highlighted in grey. For a better
comprehension, there is a detailed example for each record description following the table.
Example trace files of different data interchanges are added at the end of this protocol description.
Message header record

Every message starts with a Message Header Record. This record is mandatory.
No. Field Content Comment

1 Record type ID H The character H identifies the record as a
message header record.
2 Field delimiter | Defines the delimiters to be used throughout
Repeat delimiter \ the subsequent records of the message.
Component delimiter ^
Escape delimiter &
3 Message control ID
Not used in cobas u 411 analyzer
4 Access password
5 Sender name or ID cobas u 411 First sub field contains cobas u 411, second
^serial no. sub field contains serial number (10 digits,
^software version e.g. 0000000001), third sub field contains the
^range boundary software version, and the fourth sub field
setting contains the range boundary setting
6 Sender street address
7 Reserved field
8 Sender telephone
number
9 Characteristics of
sender
10 Receiver ID
11 Comment of special
instructions
12 Processing ID P Always P (production)
13 Version no. Not used in cobas u 411 analyzer
14 Date and time of Date and time (format YYYYMMDDhhmmss)
message example 20110315101822)
Example:
No: Frame 1 2 3 4 5 6 7 8 9 10
Content: [STX] 1 H | \^& | | | cobasu411^8^3.2.0.1001Înt | | | | | |
No: 11 12 13 14 CR Frame
Content: | | P | | 20110315101822 [CR] [ETX]71[CR][LF]
Version 2.3 16 / 54
Patient information record

Although the cobas u 411 analyzer does not save any patient data, the Patient Information
Record is mandatory by the ASTM plus standard. Therefore, the cobas u 411 analyzer simply
transmits an empty record.

1 Record type ID P The character P identifies the record as a
patient record.
2 Sequence no. 1 The cobas u 411 analyzer sends one order
per transmission only. Therefore this value is
always 1.
3 .. 35 All further fields are not supported and not transmitted
Example:
No: Frame 1 2 CR Frame
Content: [STX] 2 P | 1 [CR] [ETX]3F[CR][LF]
Order record
In the Order Record general sample information such as sample ID, sequence number, etc. will
be transmitted.

1 Record type ID O The character O identifies the record as an
order record.
always 1.
3 Specimen ID sample ID ID of the sample, numerical or
alphanumerical value (max 13 digits)
4 Instrument sample no. The cobas u 411 analyzer supports only
specimen ID ^rack ID sample no. and data carrier type (SAMPLE
^position no. for patient samples, CONTROL for control
ôperator ID samples).For order download, the sample no.
^data carrier type is not needed and is ignored.
5 Universal test ID Not used in cobas u 411 analyzer
6 Priority R Always R (routine sample)
The cobas u 411 analyzer does not support
emergency samples
7 Request / ordered
date and time
8 Specimen
collection date and
time
9 Collection end time
10 Collection volume
11 Collector ID
12 Action code X or X\Q Always X, in case of control samples X\Q
13 Danger code
14 Relevant clinical Not used in cobas u 411 analyzer
information
15 Date / time Date and time (format YYYYMMDDhhmmss)
specimen received example 20110315101822
Version 2.3 17 / 54
Example patient sample:

No: Frame 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Content: [STX] 3 O | 1 | 001 | 1^^^^SAMPLE | | R | | | | | | X | | |
No: 15 CR Frame
Content: 20110315101754 [CR] [ETX]F0[CR][LF]
Example control sample:

No: Frame 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Content: [STX] 3 O | 1 | | 0^^^^CONTROL | | R | | | | | | X\Q | | |
No: 15 CR Frame
Content: 20110315100924 [CR] [ETX]68[CR][LF]
Result record
The cobas u 411 analyzer uses the Result Record to transmit the measurement data. Each test
needs a separate Result Record. Flags are sent in the Comment Record and must be observed.

1 Record type ID R The character R identifies the record as a
result record.
2 Sequence no. 1 - 1000 The cobas u 411 stores up to 1000 results
(color, clarity and sediment parameters
included)
3 Universal test ID test no. Test no. list (e.g. 1.000) see 6.4
^test code Test code (e.g. SG) see 6.4
4 Data or result value Result value: numerical value (e.g. 1.030) or
measurement value ârbitrary value alphanumerical value (e.g. neg)
Arbitrary value: additional, this subfield is
only used if arbitrary values are used in
combination with others (Conventional or SI);
arbitrary values for color and clarity dont
exist
5 Unit unit Alphanumerical value (e.g. mg/dl), only if
result can be represented by several units.
ASCII characters from 1-127 are supported
character is translated to a u character.
6 Reference ranges Not used in cobas u 411 analyzer, not
equivalent to range tables used in cobas u
411 analyzer
7 Result abnormal flag Not used in cobas u 411 analyzer,
abnormal flags are sent in the comment
record
8 Nature of
abnormality testing
9 Result status
10 Date of change in
instrument
normative values or
units
11 Operator ID user ID User ID of operator who performed the test
Version 2.3 18 / 54
Example:
No: Frame 1 2 3 4 5 6 7 8 9 10 11 CR Frame
Content: [STX] 4 R | 1 | 1^SG | 1.005 | | | | | | | service [CR] [ETX]AD
No: Frame
Content: [CR][LF]
Comment record
The cobas u 411 analyzer uses the Comment Record to transmit the sample flags and result
flags. When at least one flag is present, this record will be attached to all corresponding Result
Records of the sample.

1 Record type ID C The character C identifies the record as a
comment record.
2 Sequence no. 1 - 1000 The cobas u 411 analyzer uses the same
sequence number as the prior result record.
3 Comment source I Always I (clinical instrument system)
4 Comment text sample flags / The cobas u 411 analyzer uses the
sample result flags comment record only for transmitting flags,
each flag is separated with the ^ delimiter.
5 Comment type I Always I (instrument flag(s) comment)
decisions!
Read the chapter 2.4 Flags for detailed information!
Example (result record with additional comment record edited result: !-Flag):
Content: [STX]5R|2|2^pH|6|||||||service[CR][ETX]10[CR][LF] Result Record
No: Frame 1 2 3 4 5 CR Frame

Content: [STX] 6 C | 2 | I | ! | I [CR] [ETX]5E[CR][LF] Comment Record
Example (result record with additional comment record abnormal and sieve: *-Flag and S-Flag):
Content: [STX]5R|2|2^pH|8|||||||service[CR][ETX]12[CR][LF] Result Record

Content: [STX] 6 C | 2 | I | *^S | I [CR] [ETX]18[CR][LF] Comment Record
Example (result record with additional comment record test strip error: T-Flag):
Content: [STX]4R|1|1^SG||||||||service[CR][ETX]B9[CR][LF] Result Record

Content: [STX] 5 C | 1 | I | T | I [CR] [ETX]8F[CR][LF] Comment Record
Version 2.3 19 / 54
Manufacturer record (raw result record - RR)

The cobas u 411 analyzer uses the Manufacturer Record RR to transmit raw data for special
application (e.g. evaluation / research). It is possible to activate this application via the user
interface (Utilities / Tools2 / Service Raw Data to Host ON)

1 Record type ID M The character M identifies the record as a
manufacturer record.
2 Sequence no. 1 - 1000 Running number
3 Record type sub-ID RR Always RR (raw result)
4 Universal test ID test no.^ Test no. list (e.g. 1) see 6.4
test code Test code (e.g. SG) see 6.4
5 Test frequency frequency LED frequency (e.g. green)
blue (~470 nm)
green (~555 nm)
orange (~620 nm)
6 Raw result value reflectance Floating number in the format ##.## (e.g.
88.06)
Example:
No: Frame 1 2 3 4 5 6 CR Frame
Content: [STX] 0 M | 1 | RR | 11^COM | blue | 78.19 [CR] [ETX]1C[CR][LF]
Manufacturer record (result context record - RC)

The cobas u 411 analyzer uses the Result Context Record for the transmission of additional
information for patients or controls.

2 Sequence no. 1 Running number
3 Record type sub-ID RC Always RC (result context)
4 Calibration strip lot e.g. 4567 Numerical or alphanumerical value
number
5 Calibration strip e.g. 20061231 Date and time format (YYYYMMDD)
expiration date
6 Test strip lot number e.g. 1234 Numerical or alphanumerical value
7 Test strip expiration e.g. 20061231 Date and time format (YYYYMMDD)
date
8 Control name e.g. ctrl Patient sample: always empty
Control sample: control name
9 Control lot number e.g. 7890 Patient sample: always empty
Control sample: control lot no
10 Control expiration date e.g. 20061231 Patient sample: always empty
Control sample: control expiration date
Date and time format (YYYYMMDD)

No: Frame 1 2 3 4 5 6 7 8 9 10 CR
Content: [STX] 0 M | 1 | RC | | | 2001601 | 20111001 | | | [CR]
No: Frame
Content: [ETX]8F[CR][LF]
Version 2.3 20 / 54

No: Frame 1 2 3 4 5 6 7 8 9
Content: [STX] 7 M | 1 | RC | | | 2001601 | 20111001 | ctrl | 123456 |
No: 10 CR Frame
Content: 20111001 [CR] [ETX]06[CR][LF]
Request information record (request for worklist download)

The cobas u 411 analyzer uses the Request Information Record to request the work list from the
host system. The host system must answer within 3 seconds.

1 Record type ID Q The character Q identifies the record as a
request information record.
2 Sequence no. 1 Always 1, using the cobas u 411 analyzer
3 Record type sub-ID ÂLL Request for all orders
Example:
No: Frame 1 2 3 CR Frame
Content: [STX] 2 Q | 1 | ÂLL [CR] [ETX]F3[CR][LF]
Termination record
Each message is closed with the Message Terminator Record. This record is mandatory.

1 Record type ID L The character L identifies the record as a
message terminator record.
2 Sequence no. 1 Per definition always 1
3 Termination code N Always N (normal termination)
Example:
No: Frame 1 2 3 CR Frame
Content: [STX] 1 L | 1 | N [CR] [ETX]04[CR][LF]
Scientific record
This type of record is not used in cobas u 411 analyzer (the character S would identify the record
as a scientific record).
Version 2.3 21 / 54
6.1.4 ASTM plus trace file examples
Example: Upload sample result without raw data

- Sample 1
- Sample 2 (with !-flag for pH)
- Sample 3 (with *- and S-Flag for pH and sediment data for ERY and LEU)
- Sample 4 (with T-flag)
Example: Upload sample result with raw data
- Sample 1
- Sample 3 (with *- and S-Flag for pH and sediment data for ERY and LEU)
Example: Upload control result without raw data
Example: Upload control result with raw data
Example: Worklist download

Sample 1
u411 10:17:03,686 [ENQ]

Host 10:17:03,686 [ACK]
u411 10:17:03,795 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315101822[CR][ETX]71[CR][LF]
Host 10:17:03,811 [ACK]
u411 10:17:03,842 [STX]2P|1[CR][ETX]3F[CR][LF]
Host 10:17:03,858 [ACK]
u411 10:17:03,936 [STX]3O|1|001|1^^^^SAMPLE||R||||||X|||201103151
01754[CR][ETX]F0[CR][LF]
Host 10:17:03,951 [ACK]
u411 10:17:04,029 [STX]4R|1|1^SG|1.005|||||||service[CR][ETX]AD[C
R][LF]
Host 10:17:04,029 [ACK]
u411 10:17:04,108 [STX]5R|2|2^pH|7|||||||service[CR][ETX]11[CR][L
F]
Host 10:17:04,108 [ACK]
u411 10:17:04,186 [STX]6R|3|3^LEU|neg|||||||service[CR][ETX]45[CR
][LF]
Host 10:17:04,186 [ACK]
u411 10:17:04,264 [STX]7R|4|4^NIT|neg|||||||service[CR][ETX]4D[CR
][LF]
Host 10:17:04,264 [ACK]
u411 10:17:04,342 [STX]0R|5|5^PRO|neg|||||||service[CR][ETX]4E[CR
][LF]
Host 10:17:04,342 [ACK]
u411 10:17:04,420 [STX]1R|6|6^GLU|neg|||||||service[CR][ETX]48[CR
][LF]
Host 10:17:04,420 [ACK]
u411 10:17:04,498 [STX]2R|7|7^KET|neg|||||||service[CR][ETX]47[CR
][LF]
Host 10:17:04,498 [ACK]
u411 10:17:04,576 [STX]3R|8|8ÛBG|neg|||||||service[CR][ETX]44[CR
][LF]
Host 10:17:04,592 [ACK]
u411 10:17:04,654 [STX]4R|9|9^BIL|neg|||||||service[CR][ETX]40[CR
][LF]
Host 10:17:04,670 [ACK]
Version 2.3 22 / 54
u411 10:17:04,764 [STX]5R|10|10ÊRY|neg|||||||service[CR][ETX]AA[

CR][LF]
Host 10:17:04,764 [ACK]
u411 10:17:04,842 [STX]6R|11|11^COL|p.yel|||||||service[CR][ETX]4
9[CR][LF]
Host 10:17:04,842 [ACK]
u411 10:17:04,920 [STX]7R|12|12^CLA||||||||service[CR][ETX]56[CR]
[LF]
Host 10:17:04,920 [ACK]
u411 10:17:04,998 [STX]0M|1|RC|||2001601|20111001|||[CR][ETX]8F[C
R][LF]
Host 10:17:04,998 [ACK]
u411 10:17:05,061 [STX]1L|1|N[CR][ETX]04[CR][LF]
Host 10:17:05,061 [ACK]
u411 10:17:05,108 [EOT]
Sample 2 (with !-flag for pH)
u411 11:01:02,941 [ENQ]

Host 11:01:02,941 [ACK]
u411 11:01:03,051 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315110221[CR][ETX]6A[CR][LF]
Host 11:01:03,051 [ACK]
Host 11:01:03,098 [ACK]
u411 11:01:03,191 [STX]3O|1|002|2^^^^SAMPLE||R||||||X|||201103151
02140[CR][ETX]E8[CR][LF]
Host 11:01:03,191 [ACK]
u411 11:01:03,270 [STX]4R|1|1^SG|1.010|||||||service[CR][ETX]A9[C
R][LF]
Host 11:01:03,285 [ACK]
F]
Host 11:01:03,363 [ACK]
u411 11:01:03,426 [STX]6C|2|I|!|I[CR][ETX]5E[CR][LF]
Host 11:01:03,426 [ACK]
][LF]
Host 11:01:03,504 [ACK]
u411 11:01:03,582 [STX]0R|4|4^NIT|neg|||||||service[CR][ETX]46[CR
][LF]
Host 11:01:03,582 [ACK]
u411 11:01:03,660 [STX]1R|5|5^PRO|neg|||||||service[CR][ETX]4F[CR
][LF]
Host 11:01:03,660 [ACK]
][LF]
Host 11:01:03,738 [ACK]
][LF]
Host 11:01:03,817 [ACK]
][LF]
Host 11:01:03,895 [ACK]
][LF]
Host 11:01:03,988 [ACK]
u411 11:01:04,051 [STX]6R|10|10ÊRY|neg|||||||service[CR][ETX]AB[
CR][LF]
Host 11:01:04,067 [ACK]
Version 2.3 23 / 54

A[CR][LF]
Host 11:01:04,145 [ACK]
u411 11:01:04,223 [STX]0R|12|12^CLA||||||||service[CR][ETX]4F[CR]
[LF]
Host 11:01:04,223 [ACK]
u411 11:01:04,301 [STX]1M|1|RC|||2001601|20111001|||[CR][ETX]90[C
R][LF]
Host 11:01:04,301 [ACK]
Host 11:01:04,363 [ACK]
u411 11:01:04,395 [EOT]
Sample 3 (with *- and S-flag for pH and sediment data for ERY and LEU)
u411 10:29:19,382 [ENQ]

Host 10:29:19,382 [ACK]
u411 10:29:19,492 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315103037[CR][ETX]71[CR][LF]
Host 10:29:19,492 [ACK]
Host 10:29:19,539 [ACK]
u411 10:29:19,632 [STX]3O|1|003|3^^^^SAMPLE||R||||||X|||201103151
02801[CR][ETX]EE[CR][LF]
Host 10:29:19,632 [ACK]
R][LF]
Host 10:29:19,710 [ACK]
F]
Host 10:29:19,789 [ACK]
u411 10:29:19,851 [STX]6C|2|I|*^S|I[CR][ETX]18[CR][LF]
Host 10:29:19,851 [ACK]
][LF]
Host 10:29:19,960 [ACK]
][LF]
Host 10:29:20,039 [ACK]
][LF]
Host 10:29:20,117 [ACK]
][LF]
Host 10:29:20,195 [ACK]
][LF]
Host 10:29:20,273 [ACK]
][LF]
Host 10:29:20,351 [ACK]
][LF]
Host 10:29:20,429 [ACK]
CR][LF]
Host 10:29:20,523 [ACK]
A[CR][LF]
Host 10:29:20,617 [ACK]
Version 2.3 24 / 54

[LF]
Host 10:29:20,695 [ACK]
u411 10:29:20,773 [STX]1R|13|51ÊRY|5-10|||||||service[CR][ETX]37
[CR][LF]
Host 10:29:20,773 [ACK]
u411 10:29:20,867 [STX]2R|14|52^LEU|11-20|||||||service[CR][ETX]
7E[CR][LF]
Host 10:29:20,867 [ACK]
u411 10:29:20,945 [STX]3M|1|RC|||2001601|20111001|||[CR][ETX]92[C
R][LF]
Host 10:29:20,945 [ACK]
Host 10:29:21,007 [ACK]
u411 10:29:21,054 [EOT]
Sample 4 (with T-flag)
u411 10:31:58,340 [ENQ]

Host 10:31:58,340 [ACK]
u411 10:31:58,450 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315103316[CR][ETX]71[CR][LF]
Host 10:31:58,465 [ACK]
Host 10:31:58,528 [ACK]
u411 10:31:58,621 [STX]3O|1|004|4^^^^SAMPLE||R||||||X|||201103151
Host 10:31:58,621 [ACK]
u411 10:31:58,700 [STX]4R|1|1^SG||||||||service[CR][ETX]B9[CR][LF
]
Host 10:31:58,700 [ACK]
u411 10:31:58,762 [STX]5C|1|I|T|I[CR][ETX]8F[CR][LF]
Host 10:31:58,762 [ACK]
u411 10:31:58,840 [STX]6R|2|2^pH||||||||service[CR][ETX]DB[CR][LF
]
Host 10:31:58,840 [ACK]
u411 10:31:58,903 [STX]7C|2|I|T|I[CR][ETX]92[CR][LF]
Host 10:31:58,903 [ACK]
u411 10:31:58,981 [STX]0R|3|3^LEU||||||||service[CR][ETX]05[CR][L
F]
Host 10:31:58,981 [ACK]
u411 10:31:59,043 [STX]1C|3|I|T|I[CR][ETX]8D[CR][LF]
Host 10:31:59,059 [ACK]
u411 10:31:59,121 [STX]2R|4|4^NIT||||||||service[CR][ETX]0E[CR][L
F]
Host 10:31:59,137 [ACK]
Host 10:31:59,200 [ACK]
u411 10:31:59,278 [STX]4R|5|5^PRO||||||||service[CR][ETX]18[CR][L
F]
Host 10:31:59,278 [ACK]
Host 10:31:59,340 [ACK]
u411 10:31:59,418 [STX]6R|6|6^GLU||||||||service[CR][ETX]13[CR][L
F]
Host 10:31:59,418 [ACK]
Host 10:31:59,481 [ACK]
u411 10:31:59,559 [STX]0R|7|7^KET||||||||service[CR][ETX]0B[CR][L
F]
Version 2.3 25 / 54
Host 10:31:59,559 [ACK]

Host 10:31:59,621 [ACK]
u411 10:31:59,700 [STX]2R|8|8ÛBG||||||||service[CR][ETX]09[CR][L
F]
Host 10:31:59,715 [ACK]
Host 10:31:59,778 [ACK]
u411 10:31:59,840 [STX]4R|9|9^BIL||||||||service[CR][ETX]06[CR][L
F]
Host 10:31:59,856 [ACK]
Host 10:31:59,918 [ACK]
u411 10:31:59,996 [STX]6R|10|10ÊRY||||||||service[CR][ETX]71[CR]
[LF]
Host 10:31:59,996 [ACK]
u411 10:32:00,059 [STX]7C|10|I|T|I[CR][ETX]C1[CR][LF]
Host 10:32:00,059 [ACK]
u411 10:32:00,137 [STX]0R|11|11^COL||||||||service[CR][ETX]5B[CR]
[LF]
Host 10:32:00,137 [ACK]
u411 10:32:00,200 [STX]1C|11|I|T|I[CR][ETX]BC[CR][LF]
Host 10:32:00,200 [ACK]
[LF]
Host 10:32:00,278 [ACK]
u411 10:32:00,356 [STX]3M|1|RC|||2001601|20111001|||[CR][ETX]92[C
R][LF]
Host 10:32:00,371 [ACK]
Host 10:32:00,434 [ACK]
u411 10:32:00,465 [EOT]

Sample 1
u411 10:36:26,562 [ENQ]

Host 10:36:26,562 [ACK]
u411 10:36:26,672 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315103745[CR][ETX]77[CR][LF]
Host 10:36:26,672 [ACK]
Host 10:36:26,734 [ACK]
u411 10:36:26,828 [STX]3O|1|001|1^^^^SAMPLE||R||||||X|||201103151
Host 10:36:26,828 [ACK]
u411 10:36:26,906 [STX]4R|1|1^SG|1.005|||||||service[CR][ETX]AD[C
R][LF]
Host 10:36:26,906 [ACK]
F]
Host 10:36:27,000 [ACK]
][LF]
Host 10:36:27,078 [ACK]
][LF]
Host 10:36:27,156 [ACK]
Version 2.3 26 / 54
][LF]
Host 10:36:27,234 [ACK]
][LF]
Host 10:36:27,312 [ACK]
][LF]
Host 10:36:27,390 [ACK]
][LF]
Host 10:36:27,468 [ACK]
][LF]
Host 10:36:27,547 [ACK]
CR][LF]
Host 10:36:27,625 [ACK]
9[CR][LF]
Host 10:36:27,718 [ACK]
[LF]
Host 10:36:27,797 [ACK]
u411 10:36:27,875 [STX]0M|1|RR|11^COM|blue|78.19[CR][ETX]1C[CR][L
F]
Host 10:36:27,875 [ACK]
u411 10:36:27,953 [STX]1M|2|RR|11^COM|green|76.80[CR][ETX]83[CR][
LF]
Host 10:36:27,953 [ACK]
u411 10:36:28,031 [STX]2M|3|RR|11^COM|orange|76.23[CR][ETX]ED[CR]
[LF]
Host 10:36:28,031 [ACK]
u411 10:36:28,109 [STX]3M|4|RR|10ÊRY|green|69.07[CR][ETX]98[CR][
LF]
Host 10:36:28,109 [ACK]
u411 10:36:28,187 [STX]4M|5|RR|10ÊRY|orange|73.75[CR][ETX]05[CR]
[LF]
Host 10:36:28,187 [ACK]
u411 10:36:28,265 [STX]5M|6|RR|3^LEU|green|77.50[CR][ETX]61[CR][L
F]
Host 10:36:28,265 [ACK]
u411 10:36:28,343 [STX]6M|7|RR|4^NIT|green|77.37[CR][ETX]6E[CR][L
F]
Host 10:36:28,359 [ACK]
u411 10:36:28,422 [STX]7M|8|RR|7^KET|green|61.63[CR][ETX]64[CR][L
F]
Host 10:36:28,437 [ACK]
u411 10:36:28,515 [STX]0M|9|RR|6^GLU|green|78.90[CR][ETX]69[CR][L
F]
Host 10:36:28,515 [ACK]
u411 10:36:28,593 [STX]1M|10|RR|5^PRO|orange|70.13[CR][ETX]F8[CR]
[LF]
Host 10:36:28,593 [ACK]
u411 10:36:28,672 [STX]2M|11|RR|8ÛBG|green|75.19[CR][ETX]8A[CR][
LF]
Host 10:36:28,672 [ACK]
u411 10:36:28,750 [STX]3M|12|RR|9^BIL|green|74.79[CR][ETX]8B[CR][
LF]
Host 10:36:28,750 [ACK]
u411 10:36:28,828 [STX]4M|13|RR|2^pH|green|36.18[CR][ETX]5E[CR][L
F]
Version 2.3 27 / 54
Host 10:36:28,828 [ACK]

u411 10:36:28,906 [STX]5M|14|RR|2^pH|orange|25.07[CR][ETX]C7[CR][
LF]
Host 10:36:28,906 [ACK]
u411 10:36:28,984 [STX]6M|15|RR|1^SG|orange|06.51[CR][ETX]A8[CR][
LF]
Host 10:36:29,000 [ACK]
u411 10:36:29,062 [STX]7M|16|RC|||2001601|20111001|||[CR][ETX]CC[
CR][LF]
Host 10:36:29,078 [ACK]
Host 10:36:29,140 [ACK]
u411 10:36:29,187 [EOT]
u411 10:59:53,165 [ENQ]

Host 10:59:53,165 [ACK]
u411 10:59:53,274 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315110111[CR][ETX]68[CR][LF]
Host 10:59:53,274 [ACK]
Host 10:59:53,321 [ACK]
u411 10:59:53,415 [STX]3O|1|002|2^^^^SAMPLE||R||||||X|||201103151
02140[CR][ETX]E8[CR][LF]
Host 10:59:53,431 [ACK]
R][LF]
Host 10:59:53,509 [ACK]
F]
Host 10:59:53,587 [ACK]
u411 10:59:53,649 [STX]6C|2|I|!|I[CR][ETX]5E[CR][LF]
Host 10:59:53,649 [ACK]
][LF]
Host 10:59:53,728 [ACK]
][LF]
Host 10:59:53,806 [ACK]
][LF]
Host 10:59:53,884 [ACK]
][LF]
Host 10:59:53,962 [ACK]
][LF]
Host 10:59:54,040 [ACK]
][LF]
Host 10:59:54,134 [ACK]
][LF]
Host 10:59:54,212 [ACK]
CR][LF]
Host 10:59:54,290 [ACK]
A[CR][LF]
Version 2.3 28 / 54
Host 10:59:54,368 [ACK]

[LF]
Host 10:59:54,446 [ACK]
u411 10:59:54,525 [STX]1M|1|RR|11^COM|blue|77.14[CR][ETX]17[CR][L
F]
Host 10:59:54,525 [ACK]
LF]
Host 10:59:54,603 [ACK]
u411 10:59:54,681 [STX]3M|3|RR|11^COM|orange|74.93[CR][ETX]F3[CR]
[LF]
Host 10:59:54,681 [ACK]
u411 10:59:54,759 [STX]4M|4|RR|10ÊRY|green|66.56[CR][ETX]9A[CR][
LF]
Host 10:59:54,775 [ACK]
[LF]
Host 10:59:54,853 [ACK]
F]
Host 10:59:54,931 [ACK]
u411 10:59:55,009 [STX]7M|7|RR|4^NIT|green|78.86[CR][ETX]74[CR][L
F]
Host 10:59:55,009 [ACK]
F]
Host 10:59:55,087 [ACK]
u411 10:59:55,165 [STX]1M|9|RR|6^GLU|green|78.04[CR][ETX]65[CR][L
F]
Host 10:59:55,165 [ACK]
[LF]
Host 10:59:55,243 [ACK]
u411 10:59:55,322 [STX]3M|11|RR|8ÛBG|green|75.26[CR][ETX]89[CR][
LF]
Host 10:59:55,322 [ACK]
u411 10:59:55,400 [STX]4M|12|RR|9^BIL|green|74.40[CR][ETX]80[CR][
LF]
Host 10:59:55,415 [ACK]
u411 10:59:55,478 [STX]5M|13|RR|2^pH|green|35.32[CR][ETX]5A[CR][L
F]
Host 10:59:55,494 [ACK]
LF]
Host 10:59:55,572 [ACK]
u411 10:59:55,650 [STX]7M|15|RR|1^SG|orange|05.68[CR][ETX]B0[CR][
LF]
Host 10:59:55,650 [ACK]
u411 10:59:55,728 [STX]0M|16|RC|||2001601|20111001|||[CR][ETX]C5[
CR][LF]
Host 10:59:55,728 [ACK]
Host 10:59:55,790 [ACK]
u411 10:59:55,837 [EOT]
Sample 3 (with *- and S-flag for pH and sediment data for ERY and LEU)
u411 10:37:48,828 [ENQ]

Host 10:37:48,843 [ACK]
Version 2.3 29 / 54
u411 10:37:48,937 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||

||P||20110315103907[CR][ETX]77[CR][LF]
Host 10:37:48,953 [ACK]
Host 10:37:49,000 [ACK]
u411 10:37:49,093 [STX]3O|1|003|3^^^^SAMPLE||R||||||X|||201103151
02801[CR][ETX]EE[CR][LF]
Host 10:37:49,093 [ACK]
R][LF]
Host 10:37:49,172 [ACK]
F]
Host 10:37:49,250 [ACK]
u411 10:37:49,312 [STX]6C|2|I|*^S|I[CR][ETX]18[CR][LF]
Host 10:37:49,312 [ACK]
][LF]
Host 10:37:49,390 [ACK]
][LF]
Host 10:37:49,468 [ACK]
][LF]
Host 10:37:49,547 [ACK]
][LF]
Host 10:37:49,640 [ACK]
][LF]
Host 10:37:49,718 [ACK]
][LF]
Host 10:37:49,797 [ACK]
][LF]
Host 10:37:49,875 [ACK]
CR][LF]
Host 10:37:49,953 [ACK]
A[CR][LF]
Host 10:37:50,031 [ACK]
[LF]
Host 10:37:50,109 [ACK]
u411 10:37:50,187 [STX]1R|13|51ÊRY|5-10|||||||service[CR][ETX]37
[CR][LF]
Host 10:37:50,187 [ACK]
u411 10:37:50,265 [STX]2R|14|52^LEU|11-20|||||||service[CR][ETX]
7E[CR][LF]
Host 10:37:50,281 [ACK]
u411 10:37:50,343 [STX]3M|1|RR|11^COM|blue|74.73[CR][ETX]1B[CR][L
F]
Host 10:37:50,359 [ACK]
LF]
Host 10:37:50,437 [ACK]
u411 10:37:50,515 [STX]5M|3|RR|11^COM|orange|73.35[CR][ETX]F0[CR]
[LF]
Host 10:37:50,515 [ACK]
Version 2.3 30 / 54
u411 10:37:50,593 [STX]6M|4|RR|10ÊRY|green|68.63[CR][ETX]9C[CR][

LF]
Host 10:37:50,593 [ACK]
[LF]
Host 10:37:50,672 [ACK]
u411 10:37:50,750 [STX]0M|6|RR|3^LEU|green|76.22[CR][ETX]5A[CR][L
F]
Host 10:37:50,750 [ACK]
u411 10:37:50,828 [STX]1M|7|RR|4^NIT|green|77.83[CR][ETX]6A[CR][L
F]
Host 10:37:50,828 [ACK]
u411 10:37:50,906 [STX]2M|8|RR|7^KET|green|62.44[CR][ETX]5F[CR][L
F]
Host 10:37:50,906 [ACK]
u411 10:37:50,984 [STX]3M|9|RR|6^GLU|green|79.26[CR][ETX]6C[CR][L
F]
Host 10:37:51,000 [ACK]
u411 10:37:51,062 [STX]4M|10|RR|5^PRO|orange|70.25[CR][ETX]FE[CR]
[LF]
Host 10:37:51,078 [ACK]
LF]
Host 10:37:51,156 [ACK]
LF]
Host 10:37:51,234 [ACK]
u411 10:37:51,312 [STX]7M|13|RR|2^pH|green|35.36[CR][ETX]60[CR][L
F]
Host 10:37:51,312 [ACK]
u411 10:37:51,390 [STX]0M|14|RR|2^pH|orange|24.20[CR][ETX]BC[CR][
LF]
Host 10:37:51,390 [ACK]
LF]
Host 10:37:51,468 [ACK]
u411 10:37:51,547 [STX]2M|16|RC|||2001601|20111001|||[CR][ETX]C7[
CR][LF]
Host 10:37:51,547 [ACK]
Host 10:37:51,625 [ACK]
u411 10:37:51,656 [EOT]
u411 10:38:09,531 [ENQ]

Host 10:38:09,531 [ACK]
u411 10:38:09,640 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315103928[CR][ETX]7A[CR][LF]
Host 10:38:09,656 [ACK]
Host 10:38:09,703 [ACK]
u411 10:38:09,781 [STX]3O|1|004|4^^^^SAMPLE||R||||||X|||201103151
Host 10:38:09,797 [ACK]
u411 10:38:09,859 [STX]4R|1|1^SG||||||||service[CR][ETX]B9[CR][LF
]
Host 10:38:09,875 [ACK]
u411 10:38:09,937 [STX]5C|1|I|T|I[CR][ETX]8F[CR][LF]
Host 10:38:09,937 [ACK]
Version 2.3 31 / 54
u411 10:38:10,015 [STX]6R|2|2^pH||||||||service[CR][ETX]DB[CR][LF

]
Host 10:38:10,015 [ACK]
Host 10:38:10,078 [ACK]
u411 10:38:10,156 [STX]0R|3|3^LEU||||||||service[CR][ETX]05[CR][L
F]
Host 10:38:10,156 [ACK]
u411 10:38:10,218 [STX]1C|3|I|T|I[CR][ETX]8D[CR][LF]
Host 10:38:10,218 [ACK]
u411 10:38:10,297 [STX]2R|4|4^NIT||||||||service[CR][ETX]0E[CR][L
F]
Host 10:38:10,297 [ACK]
Host 10:38:10,359 [ACK]
u411 10:38:10,437 [STX]4R|5|5^PRO||||||||service[CR][ETX]18[CR][L
F]
Host 10:38:10,453 [ACK]
Host 10:38:10,515 [ACK]
u411 10:38:10,593 [STX]6R|6|6^GLU||||||||service[CR][ETX]13[CR][L
F]
Host 10:38:10,593 [ACK]
Host 10:38:10,656 [ACK]
u411 10:38:10,734 [STX]0R|7|7^KET||||||||service[CR][ETX]0B[CR][L
F]
Host 10:38:10,734 [ACK]
Host 10:38:10,797 [ACK]
u411 10:38:10,875 [STX]2R|8|8ÛBG||||||||service[CR][ETX]09[CR][L
F]
Host 10:38:10,875 [ACK]
Host 10:38:10,937 [ACK]
u411 10:38:11,000 [STX]4R|9|9^BIL||||||||service[CR][ETX]06[CR][L
F]
Host 10:38:11,000 [ACK]
Host 10:38:11,078 [ACK]
u411 10:38:11,140 [STX]6R|10|10ÊRY||||||||service[CR][ETX]71[CR]
[LF]
Host 10:38:11,156 [ACK]
u411 10:38:11,218 [STX]7C|10|I|T|I[CR][ETX]C1[CR][LF]
Host 10:38:11,218 [ACK]
u411 10:38:11,297 [STX]0R|11|11^COL||||||||service[CR][ETX]5B[CR]
[LF]
Host 10:38:11,297 [ACK]
u411 10:38:11,359 [STX]1C|11|I|T|I[CR][ETX]BC[CR][LF]
Host 10:38:11,359 [ACK]
[LF]
Host 10:38:11,437 [ACK]
u411 10:38:11,515 [STX]3M|1|RR|11^COM|blue|83.49[CR][ETX]1E[CR][L
F]
Host 10:38:11,515 [ACK]
u411 10:38:11,593 [STX]4M|2|RR|11^COM|green|80.23[CR][ETX]7E[CR][
LF]
Host 10:38:11,593 [ACK]
u411 10:38:11,672 [STX]5M|3|RR|11^COM|orange|82.25[CR][ETX]EF[CR]
[LF]
Version 2.3 32 / 54
Host 10:38:11,672 [ACK]

u411 10:38:11,750 [STX]6M|4|RR|10ÊRY|green|78.72[CR][ETX]9D[CR][
LF]
Host 10:38:11,765 [ACK]
[LF]
Host 10:38:11,843 [ACK]
F]
Host 10:38:11,922 [ACK]
F]
Host 10:38:12,000 [ACK]
F]
Host 10:38:12,078 [ACK]
u411 10:38:12,156 [STX]3M|9|RR|6^GLU|green|79.73[CR][ETX]6E[CR][L
F]
Host 10:38:12,156 [ACK]
u411 10:38:12,234 [STX]4M|10|RR|5^PRO|orange|82.63[CR][ETX]03[CR]
[LF]
Host 10:38:12,234 [ACK]
u411 10:38:12,312 [STX]5M|11|RR|8ÛBG|green|80.48[CR][ETX]8B[CR][
LF]
Host 10:38:12,312 [ACK]
LF]
Host 10:38:12,406 [ACK]
F]
Host 10:38:12,484 [ACK]
LF]
Host 10:38:12,562 [ACK]
LF]
Host 10:38:12,640 [ACK]
u411 10:38:12,718 [STX]2M|16|RC|||2001601|20111001|||[CR][ETX]C7[
CR][LF]
Host 10:38:12,718 [ACK]
Host 10:38:12,765 [ACK]
u411 10:38:12,812 [EOT]

u411 10:08:06,437 [ENQ]
Host 10:08:06,437 [ACK]
u411 10:08:06,547 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315100925[CR][ETX]74[CR][LF]
Host 10:08:06,562 [ACK]
Host 10:08:06,625 [ACK]
u411 10:08:06,703 [STX]3O|1||0^^^^CONTROL||R||||||X\Q|||201103151
00924[CR][ETX]68[CR][LF]
Host 10:08:06,718 [ACK]
R][LF]
Host 10:08:06,812 [ACK]
Version 2.3 33 / 54
F]
Host 10:08:06,890 [ACK]
][LF]
Host 10:08:06,968 [ACK]
][LF]
Host 10:08:07,047 [ACK]
][LF]
Host 10:08:07,125 [ACK]
][LF]
Host 10:08:07,203 [ACK]
][LF]
Host 10:08:07,281 [ACK]
][LF]
Host 10:08:07,359 [ACK]
][LF]
Host 10:08:07,453 [ACK]
CR][LF]
Host 10:08:07,531 [ACK]
9[CR][LF]
Host 10:08:07,609 [ACK]
u411 10:08:07,703 [STX]7M|1|RC|||2001601|20111001|ctrl|123456|201
11001[CR][ETX]06[CR][LF]
Host 10:08:07,703 [ACK]
Host 10:08:07,750 [ACK]
u411 10:08:07,796 [EOT]

u411 10:34:59,593 [ENQ]
Host 10:34:59,609 [ACK]
u411 10:34:59,703 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315103618[CR][ETX]76[CR][LF]
Host 10:34:59,718 [ACK]
Host 10:34:59,781 [ACK]
u411 10:34:59,875 [STX]3O|1||0^^^^CONTROL||R||||||X\Q|||201103151
00924[CR][ETX]68[CR][LF]
Host 10:34:59,875 [ACK]
R][LF]
Host 10:34:59,953 [ACK]
F]
Host 10:35:00,031 [ACK]
][LF]
Host 10:35:00,109 [ACK]
][LF]
Host 10:35:00,187 [ACK]
Version 2.3 34 / 54

][LF]
Host 10:35:00,265 [ACK]
][LF]
Host 10:35:00,359 [ACK]
][LF]
Host 10:35:00,437 [ACK]
][LF]
Host 10:35:00,515 [ACK]
][LF]
Host 10:35:00,593 [ACK]
CR][LF]
Host 10:35:00,672 [ACK]
9[CR][LF]
Host 10:35:00,750 [ACK]
u411 10:35:00,828 [STX]7M|1|RR|11^COM|blue|74.53[CR][ETX]1D[CR][L
F]
Host 10:35:00,828 [ACK]
LF]
Host 10:35:00,906 [ACK]
u411 10:35:00,984 [STX]1M|3|RR|11^COM|orange|72.37[CR][ETX]ED[CR]
[LF]
Host 10:35:00,984 [ACK]
u411 10:35:01,062 [STX]2M|4|RR|10ÊRY|green|68.44[CR][ETX]97[CR][
LF]
Host 10:35:01,078 [ACK]
u411 10:35:01,140 [STX]3M|5|RR|10ÊRY|orange|73.07[CR][ETX]FF[CR]
[LF]
Host 10:35:01,156 [ACK]
F]
Host 10:35:01,234 [ACK]
F]
Host 10:35:01,312 [ACK]
F]
Host 10:35:01,390 [ACK]
u411 10:35:01,468 [STX]7M|9|RR|6^GLU|green|77.33[CR][ETX]6C[CR][L
F]
Host 10:35:01,468 [ACK]
[LF]
Host 10:35:01,547 [ACK]
LF]
Host 10:35:01,625 [ACK]
u411 10:35:01,703 [STX]2M|12|RR|9^BIL|green|73.60[CR][ETX]7F[CR][
LF]
Host 10:35:01,718 [ACK]
F]
Host 10:35:01,797 [ACK]
Version 2.3 35 / 54
LF]
Host 10:35:01,875 [ACK]
LF]
Host 10:35:01,953 [ACK]
u411 10:35:02,047 [STX]6M|16|RC|||2001601|20111001|ctrl|123456|20
111001[CR][ETX]3B[CR][LF]
Host 10:35:02,047 [ACK]
u411 10:35:02,093 [STX]7L|1|N[CR][ETX]0A[CR][LF]
Host 10:35:02,093 [ACK]
u411 10:35:02,140 [EOT]

The cobas u 411 analyzer starts the request
u411 10:41:34,530 [ENQ]
Host 10:41:34,655 [ACK]
u411 10:41:34,686 [STX]1H|\^&|||cobasu411^8^3.2.0.1001Înt|||||
||P||20110315104252[CR][ETX]71[CR][LF]
Host 10:41:35,061 [ACK]
u411 10:41:35,093 [STX]2Q|1|ÂLL[CR][ETX]F3[CR][LF]
Host 10:41:35,468 [ACK]
Host 10:41:35,874 [ACK]
u411 10:41:35,905 [EOT]
Host sends the sample ID 012

Host 10:41:37,296 [ENQ]
u411 10:41:37,311 [ACK]
Host 10:41:37,515 [STX]1H|\^&|||Host|||||||P[CR][ETX]AB[CR][LF]
u411 10:41:37,561 [ACK]
Host 10:41:37,921 [STX]2P|1[CR][ETX]3F[CR][LF]
u411 10:41:37,952 [ACK]
Host 10:41:38,327 [STX]3O|1|012|^^^^SAMPLE||R||||||X|||2011031510
4136[CR][ETX]BE[CR][LF]
u411 10:41:38,624 [ACK]
Host 10:41:38,733 [STX]4L|1|N[CR][ETX]07[CR][LF]
u411 10:41:38,765 [ACK]
Host 10:41:39,140 [EOT]
Version 2.3 36 / 54
Description of the supported protocols - ASTM Urisys 2400 protocol
6.2 ASTM Urisys 2400 protocol
6.2.1 General
This chapter describes the ASTM Urisys 2400 protocol. This protocol consists of Records,
Frames and Messages.
A record is the smallest logical group of information
A frame is a defined data structure that contains a data block to be transmitted
Messages are a collection of several records packed in frames into a logical unit
Note: The ASTM Urisys 2400 protocol is based on the ASTM protocol specification.
Records
A record is the smallest logical group of information. The ASTM Urisys 2400 protocol supports the
following records:
Identifier Name Description

H Header record First record in every message, mandatory
P Patient information This record is used to download patient demographics
record from the host system to the analyzer. With the cobas u
411 analyzer no patient data can be handled. Therefore
the patient record is always empty.
O Order record This record defines the attributes of a particular order.
R Result record Measurement data
C Comment record This record is used for the transmission of sample flags
and result flags.
M Manufacturer record This record is used for the transmission of raw data,
additional information for patient, control results.
Q Request information Worklist request (query). Not supported.
record Does not exist in ASTM URISYS 2400 protocol.
L Termination record Last record in every message, mandatory
Frames
A frame is a defined data structure that encapsulates a data block to be transmitted. According to
the ASTM Urisys 2400 protocol specification, a frame may contain one or more records. However,
the implementation in the cobas u 411 analyzer contains one record only. Each frame has the
following structure:
Description Start of Frame Protocol End of Check- Carriage Line feed

text No. specific text sum return
ASCII STX record ETX CR LF
(hex) (0x02) data (0x03) (0x0D) (0x0A)
The frame no. always starts at 1 and will be incremented by one for each record. If the frame
reaches no. 7, the numbering starts at 0 (zero).
Version 2.3 37 / 54
Usually, the ASTM protocol contains one record only per frame:
[STX] + Frame no. + Information (record) + [ETX] + Checksum + [CR] + [LF]
The ASTM Urisys 2400 protocol is a special implementation of ASTM. All records will be packed in
one frame. According to ASTM standard the total length of the data in one frame (message) is 247
bytes (including frame overhead). Messages longer than 240 bytes are divided into several frames,
called sub-frame. Such a sub-frame is terminated with an [ETB] character:
[STX] + Frame no. + Information 1 + [ETB] + Checksum + [CR] + [LF]

[STX] + Frame no. + Information 2 + [ETX] + Checksum + [CR] + [LF] (Msg-end, therefore [ETX])
Example:
u411 10:46:36,385 [ENQ]
Host 10:46:36,385 [ACK]
Information 1
u411 10:46:36,682 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
1|001|1^^^service^SAMPLE||R||||||X|||2011031510
1754[CR]R|1|^^^1|1.005|||||[CR]C|1|I||I[CR]R|2|
^^^2|7|||||[CR]C|2|I||I[CR]R|3|^^^3|NEG|||||[CR
]C|3|I||I[CR]R|4|^^^4|NEG|||||[CR]C|4|I||I[CR]R
|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG|||
||[CR][ETB]97[CR][LF] [ETB] Device control
Host 10:46:36,698 [ACK]
Information 2
u411 10:46:36,932 [STX]2C|6|I||I[CR]R|7|^^^7|NEG|||||[CR]C|7|I||I
[CR]R|8|^^^8|NEG|||||[CR]C|8|I||I[CR]R|9|^^^9|N
EG|||||[CR]C|9|I||I[CR]R|10|^^^10|NEG|||||[CR]C
|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11|I||I[
CR]R|12|^^^12||||||X[CR]C|12|I||I[CR]L|1|[CR][E
TX]D3[CR][LF] [ETX] End of text
Host 10:46:36,932 [ACK]
u411 10:46:36,979 [EOT]
Messages
Messages are collections of several records packed in frames into a logical unit. A message starts
with a Header Record (H) and ends with the Termination Record (L).
Version 2.3 38 / 54
6.2.2 Communication startup
Only the cobas u 411 analyzer can start a communication. The cobas u 411 analyzer is always
the master, the host system the slave.
A communication is initialized when the ASCII character ENQ

(Hex 05) is sent to the host system.
The host system (slave) acknowledges its readiness to

communicate by sending the ASCII character ACK (Hex 06) or
if it is not ready the ASCII character NAK (Hex 15).
If the host system has positively acknowledged its readiness to

communicate (ACK), the cobas u 411 analyzer transmits the
message frame by frame.
cobas u 411
The host system confirms the frame reception with ACK.
The host system can trigger a resending of the last sent frame
with NAK.
The communication session is terminated with the ASCII

character EOT (Hex 04).
6.2.3 Record descriptions
In this chapter, the meaning of the fields in individual records will be explained in the form of tables.
Fields which are not supported by the cobas u 411 analyzer are highlighted in grey.
For a better comprehension, there is a detailed example for each record description following the
table. Example trace files of different data interchanges are added at the end of this protocol
description.
Version 2.3 39 / 54
Message header record

Each message starts with the Message Header Record. This record is mandatory.

1 Record type ID H The character H identifies the record as a
message header record.
2 Field Delimiter | Defines the delimiters to be used
Repeat Delimiter \ throughout the subsequent records of the
Component Delimiter ^ message.
Escape Delimiter &
3 Message control ID
4 Access password
5 Sender name or ID serial no. Serial number of the analyzer
6 Sender street address
7 Reserved field
8 Sender telephone
number
9 Characteristics of Not used in cobas u 411 analyzer
sender
10 Receiver ID
11 Comment of special
instructinos
12 Processing ID P Always P (production)
13 Version number software version e.g. 3.2.0.1001
14 Date and time of Not supported and not transmitted
message
Example:
No: 1 2 3 4 5 6 7 8 9 10 11 12 13 CR
Content: H | \^& | | | 8 | | | | | | | P | 3.2.0.1001 [CR]
Patient information record

Although the cobas u 411 analyzer does not record any patient data, the Patient Information
Record is mandatory by the ASTM standard. For this reason the cobas u 411 analyzer transmits
an empty record.

1 Record type ID P The character P identifies the record as a
patient record.
always 1.
Example:
No: 1 2 CR
Content: P | 1 [CR]
Version 2.3 40 / 54
Order record
The cobas u 411 analyzer uses the Order Record to transmit general sample information such as
sample ID, sequence no. etc.

1 Record type ID O The character O identifies the record as an
order record.
always 1.
3 Specimen ID sample ID ID of the sample, numerical or
alphanumerical value (max. 13 digits)
4 Instrument sample no The cobas u 411 analyzer supports only
specimen ID ^rack ID sample no, user ID and data carrier type
^position no. (SAMPLE for patient samples, CONTROL
ôperator ID for control samples. For order download the
^data carrier type sample no. is not needed and is ignored.
5 Universal test ID Not used in cobas u 411 analyzer
6 Priority R Always R (routine sample), the cobas u 411
analyzer does not support emergency
samples
7 Request/ordered
date and time
8 Specimen collection
date and time Not used in cobas u 411 analyzer
9 Collection end time
10 Collection volume
11 Collector ID
12 Action code X or X\Q Always X, in case of control samples X\Q
13 Danger code Not used in cobas u 411 analyzer
14 Relevant clinical Not used in cobas u 411 analyzer
information
15 Date / time Date and time (format YYYYMMDDhhmmss)
specimen received example 20000202035009

No: 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Content: O | 1 | 001 | 1^^^service^SAMPLE | | R | | | | | | X | | |
No: 15 CR
Content: 20110315101754 [CR]

No: 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Content: O | 1 | | 0^^^^CONTROL | | R | | | | | | X\Q | | |
No: 15 CR
Content: 20110315100924 [CR]
Version 2.3 41 / 54
Result record
The cobas u 411 analyzer uses the Result Record to transmit the measurement data. Each test
needs a separate Result Record. Flags related to test strip errors (e.g. strip upside down) are sent
in this Result Record in no. 7 and no. 9. Flags related to result errors are sent in the Comment
Record and must be observed.

1 Record type ID R The character R identifies the record as a
result record.
2 Sequence no. 1 - 12 The cobas u 411 analyzer always saves 12
results (color and clarity included)
3 Universal test ID ^ Test no list (fixation):
^ 1: SG
^test no. 2: pH
3: LEU
4: NIT
5: PRO
6: GLU
7: KET
8: UBG
9: BIL
10: ERY
11: COL
12: CLA
4 Data or result value Result value:
measurement value Numerical value (e.g. 1.030) or
alphanumerical value (e.g. neg.)
Important: To be compatible with the

original URISYS 2400 analyzer, the following
conversions are done in the driver:
pos POS
pos. POS
neg NEG
neg. NEG
norm NORM
norm. NORM
5 Unit unit Alphanumerical value (e.g. mg/dL), only if
result can be represented by several units
Important: All l letters, which stand for

liter, are converted to capital letters (e. g.
ml mL) and character is translated to a
u character.
6 Reference ranges Not used in cobas u 411 analyzer
7 Result abnorm. flag Flag R: Test Strip Error
8 Nature of Not used in cobas u 411 analyzer
abnormality testing
9 Result status Flag X: Order cannot be done. Usually, in
combination with result abnormal flag (R)
Version 2.3 42 / 54
Example:
No: 1 2 3 4 5 6 7 8 9 CR
Content: R | 1 | ^^^1 | 1.005 | | | | | [CR]
Example (test strip error: R/X-Flag):

No: 1 2 3 4 5 6 7 8 9 CR
Content: R | 2 | ^^^2 | | | | R | | X [CR]
Comment record
Every Result Record has a Comment Record attached. The cobas u 411 analyzer uses the
Comment Record to transmit the sample flags and result flags. In case of no flag, an empty
Comment Record is transmitted.

1 Record type ID C The character C identifies the record as a
comment record.
2 Sequence no. 1 The cobas u 411 analyzer uses the same
sequence number as the prior result record.
3 Comment source I Always I (clinical instrument system)
4 Comment text Sample result flags The cobas u 411 analyzer uses the
comment record only for transmitting flags,
each flag is separated with the ^ delimiter.
5 Comment type I Always I (instrument flag(s) comment)
decisions!
Read the chapter 2.4 Flags for detailed information!
Example (result record and comment record no flag):

Content: R|2|^^^2|7|||||[CR] Result record
No: 1 2 3 4 5 CR
Content: C | 2 | I | | I [CR] Comment record without flags
Example (result record and comment record edited result: !-flag):

No: 1 2 3 4 5 CR
Content: C | 2 | I | ! | I [CR] Comment record with flags
Example (result record and comment record abnormal and sieve: *-flag and S-flag):
No: 1 2 3 4 5 CR
Content: C | 2 | I | *^S | I [CR] Comment record with flags
Example (result record and comment record test strip error: R/X-flag):
Content: R|2|^^^2||||R||X[CR] Result record with flags
No: 1 2 3 4 5 CR
Content: C | 2 | I | | I [CR] Comment Record without flags
Version 2.3 43 / 54
Manufacturer record (raw result record - RR)

The cobas u 411 analyzer uses the Manufacturer Record RR to transmit raw data for special
application (e.g. evaluation / research). It is possible to activate this application via the user
interface (Utilities / Tools2 / Service Raw Data to Host ON)

2 Sequence no. 1 - 16 The cobas u 411 analyzer determines
always 16 results, running number
3 Record type sub-ID RR Always RR (raw result)
4 Raw result value reflectance floating number in the format ##.## (e.g.
88.06) fix order for raw result value:
1: COM blue
2: COM green
3: COM orange
4: ERY green
5: ERY orange
6: LEU green
7: NIT green
8: KET green
9: GLU green
10: PRO orange
11: UBG green
12: BIL green
13: pH green
14: pH orange
15: SG orange
16: not used, always 0
Example:
No: 1 2 3 4 CR
Content: M | 1 | RR | 78.19 | [CR]
Manufacturer record (result context record - RC)

The cobas u 411 analyzer uses the Manufacturer Record RC to transmit the control name as
well as the control lot number.

1 Record type ID M The character M identifies the record
as a manufacturer record.
2 Sequence no. 1 Running number
3 Record type sub-ID RC Always RC (result context)
4 Test strip cassette lot number Always empty
5 Date / time test strip cassette Always empty
set
6 Control name e.g. Ctrl Control name
7 Control identifier e.g. 123456 Control lot no
Example:
No: 1 2 3 4 5 6 7 CR
Version 2.3 44 / 54
Content: M | 1 | RC | | | ctrl | 123456 | [CR]
Termination record
Each message is closed with the Message Terminator Record. This record is mandatory.

1 Record type ID L The character L identifies the record
as a message terminator record.
2 Sequence no. 1 Per definition always 1
3 Termination code Empty if normal end of message
Example:
No: 1 2 3 CR
Content: L | 1 | [CR]
Scientific Record
This type of record is not used in cobas u 411 analyzer (the character S would identify the record
as a scientific record).
Version 2.3 45 / 54
6.2.4 ASTM Urisys 2400 trace file examples

- Sample 1
- Sample 3 (with *- and S-flag for pH)
- Sample 1
- Sample 3 (with *- and S-flag for pH)

Sample 1
u411 10:46:36,385 [ENQ]

Host 10:46:36,385 [ACK]
u411 10:46:36,682 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
1754[CR]R|1|^^^1|1.005|||||[CR]C|1|I||I[CR]R|2|
^^^2|7|||||[CR]C|2|I||I[CR]R|3|^^^3|NEG|||||[CR
|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG|||
||[CR][ETB]97[CR][LF]
Host 10:46:36,698 [ACK]
|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11|I||I[
CR]R|12|^^^12||||||X[CR]C|12|I||I[CR]L|1|[CR][E
TX]D3[CR][LF]
Host 10:46:36,932 [ACK]
u411 10:46:36,979 [EOT]
u411 11:02:07,436 [ENQ]

Host 11:02:07,436 [ACK]
u411 11:02:07,733 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
2140[CR]R|1|^^^1|1.010|||||[CR]C|1|I||I[CR]R|2|
^^^2|6|||||[CR]C|2|I|!|I[CR]R|3|^^^3|NEG|||||[C
R]C|3|I||I[CR]R|4|^^^4|NEG|||||[CR]C|4|I||I[CR]
R|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG||
|||[ETB]9E[CR][LF]
Host 11:02:07,748 [ACK]
u411 11:02:07,983 [STX]2[CR]C|6|I||I[CR]R|7|^^^7|NEG|||||[CR]C|7|
I||I[CR]R|8|^^^8|NEG|||||[CR]C|8|I||I[CR]R|9|^^
^9|NEG|||||[CR]C|9|I||I[CR]R|10|^^^10|NEG|||||[
CR]C|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11|I
||I[CR]R|12|^^^12||||||X[CR]C|12|I||I[CR]L|1|[C
R][ETX]E0[CR][LF]
Version 2.3 46 / 54
Host 11:02:07,983 [ACK]

u411 11:02:08,029 [EOT]
Sample 3 (with *- and S-flag for pH)
u411 10:48:02,056 [ENQ]

Host 10:48:02,056 [ACK]
u411 10:48:02,353 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
2801[CR]R|1|^^^1|1.010|||||[CR]C|1|I||I[CR]R|2|
^^^2|8|||||[CR]C|2|I|*^S|I[CR]R|3|^^^3|NEG|||||
[CR]C|3|I||I[CR]R|4|^^^4|NEG|||||[CR]C|4|I||I[C
R]R|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG
|||[ETB]68[CR][LF]
Host 10:48:02,353 [ACK]
u411 10:48:02,587 [STX]2||[CR]C|6|I||I[CR]R|7|^^^7|NEG|||||[CR]C|
7|I||I[CR]R|8|^^^8|NEG|||||[CR]C|8|I||I[CR]R|9|
^^^9|NEG|||||[CR]C|9|I||I[CR]R|10|^^^10|NEG||||
|[CR]C|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11
|I||I[CR]R|12|^^^12||||||X[CR]C|12|I||I[CR]L|1|
[CR][ETX]D8[CR][LF]
Host 10:48:02,603 [ACK]
u411 10:48:02,634 [EOT]
u411 10:48:41,159 [ENQ]

Host 10:48:41,159 [ACK]
u411 10:48:41,456 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
3316[CR]R|1|^^^1||||R||X[CR]C|1|I||I[CR]R|2|^^^
2||||R||X[CR]C|2|I||I[CR]R|3|^^^3||||R||X[CR]C|
3|I||I[CR]R|4|^^^4||||R||X[CR]C|4|I||I[CR]R|5|^
^^5||||R||X[CR]C|5|I||I[CR]R|6|^^^6||||R||X[CR]
C|6|I|[ETB]38[CR][LF]
Host 10:48:41,471 [ACK]
u411 10:48:41,706 [STX]2|I[CR]R|7|^^^7||||R||X[CR]C|7|I||I[CR]R|8
|^^^8||||R||X[CR]C|8|I||I[CR]R|9|^^^9||||R||X[C
R]C|9|I||I[CR]R|10|^^^10||||R||X[CR]C|10|I||I[C
R]R|11|^^^11||||R||X[CR]C|11|I||I[CR]R|12|^^^12
||||R||X[CR]C|12|I||I[CR]L|1|[CR][ETX]F1[CR][LF
]
Host 10:48:41,706 [ACK]
u411 10:48:41,753 [EOT]

Sample 1
u411 10:50:41,013 [ENQ]

Host 10:50:41,029 [ACK]
u411 10:50:41,326 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
1754[CR]R|1|^^^1|1.005|||||[CR]C|1|I||I[CR]R|2|
^^^2|7|||||[CR]C|2|I||I[CR]R|3|^^^3|NEG|||||[CR
|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG|||
Version 2.3 47 / 54
||[CR][ETB]97[CR][LF]
Host 10:50:41,326 [ACK]
|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11|I||I[
CR]R|12|^^^12||||||X[CR]C|12|I||I[CR]M|1|RR|78.
19|[CR]M|2|RR|76.80|[CR]M|3|RR|76.23|[CR]M|4|RR
|69.07|[CR]M|5|[ETB]6F[CR][LF]
Host 10:50:41,622 [ACK]
u411 10:50:41,857 [STX]3RR|73.75|[CR]M|6|RR|77.50|[CR]M|7|RR|77.3
|70.13|[CR]M|11|RR|75.19|[CR]M|12|RR|74.79|[CR]
M|13|RR|36.18|[CR]M|14|RR|25.07|[CR]M|15|RR|06.
51|[CR]M|16|RR|0|[CR]L|1|[CR][ETX]70[CR][LF]
Host 10:50:41,857 [ACK]
u411 10:50:41,904 [EOT]
u411 10:58:40,529 [ENQ]

Host 10:58:40,529 [ACK]
u411 10:58:40,826 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
2140[CR]R|1|^^^1|1.010|||||[CR]C|1|I||I[CR]R|2|
^^^2|6|||||[CR]C|2|I|!|I[CR]R|3|^^^3|NEG|||||[C
R]C|3|I||I[CR]R|4|^^^4|NEG|||||[CR]C|4|I||I[CR]
R|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG||
|||[ETB]9E[CR][LF]
Host 10:58:40,826 [ACK]
u411 10:58:41,138 [STX]2[CR]C|6|I||I[CR]R|7|^^^7|NEG|||||[CR]C|7|
I||I[CR]R|8|^^^8|NEG|||||[CR]C|8|I||I[CR]R|9|^^
^9|NEG|||||[CR]C|9|I||I[CR]R|10|^^^10|NEG|||||[
CR]C|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11|I
||I[CR]R|12|^^^12||||||X[CR]C|12|I||I[CR]M|1|RR
|77.14|[CR]M|2|RR|75.73|[CR]M|3|RR|74.93|[CR]M|
4|RR|66.56|[CR]M|5[ETB]01[CR][LF]
Host 10:58:41,138 [ACK]
u411 10:58:41,357 [STX]3|RR|65.93|[CR]M|6|RR|77.83|[CR]M|7|RR|78.
86|[CR]M|8|RR|61.32|[CR]M|9|RR|78.04|[CR]M|10|R
R|70.10|[CR]M|11|RR|75.26|[CR]M|12|RR|74.40|[CR
]M|13|RR|35.32|[CR]M|14|RR|24.09|[CR]M|15|RR|05
.68|[CR]M|16|RR|0|[CR]L|1|[CR][ETX]E1[CR][LF]
Host 10:58:41,357 [ACK]
u411 10:58:41,404 [EOT]
Sample 3 (with *- and S-flag for pH)
u411 10:51:28,177 [ENQ]

Host 10:51:28,177 [ACK]
u411 10:51:28,474 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
2801[CR]R|1|^^^1|1.010|||||[CR]C|1|I||I[CR]R|2|
^^^2|8|||||[CR]C|2|I|*^S|I[CR]R|3|^^^3|NEG|||||
[CR]C|3|I||I[CR]R|4|^^^4|NEG|||||[CR]C|4|I||I[C
R]R|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG
|||[ETB]68[CR][LF]
Host 10:51:28,474 [ACK]
Version 2.3 48 / 54
u411 10:51:28,771 [STX]2||[CR]C|6|I||I[CR]R|7|^^^7|NEG|||||[CR]C|

7|I||I[CR]R|8|^^^8|NEG|||||[CR]C|8|I||I[CR]R|9|
^^^9|NEG|||||[CR]C|9|I||I[CR]R|10|^^^10|NEG||||
|[CR]C|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11
|I||I[CR]R|12|^^^12||||||X[CR]C|12|I||I[CR]M|1|
RR|74.73|[CR]M|2|RR|73.65|[CR]M|3|RR|73.35|[CR]
M|4|RR|68.63|[CR]M[ETB]44[CR][LF]
Host 10:51:28,786 [ACK]
u411 10:51:29,021 [STX]3|5|RR|73.44|[CR]M|6|RR|76.22|[CR]M|7|RR|7
7.83|[CR]M|8|RR|62.44|[CR]M|9|RR|79.26|[CR]M|10
|RR|70.25|[CR]M|11|RR|74.40|[CR]M|12|RR|74.32|[
CR]M|13|RR|35.36|[CR]M|14|RR|24.20|[CR]M|15|RR|
05.15|[CR]M|16|RR|0|[CR]L|1|[CR][ETX]81[CR][LF]
Host 10:51:29,021 [ACK]
u411 10:51:29,067 [EOT]
u411 10:51:43,737 [ENQ]

Host 10:51:43,737 [ACK]
u411 10:51:44,034 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
3316[CR]R|1|^^^1||||R||X[CR]C|1|I||I[CR]R|2|^^^
2||||R||X[CR]C|2|I||I[CR]R|3|^^^3||||R||X[CR]C|
3|I||I[CR]R|4|^^^4||||R||X[CR]C|4|I||I[CR]R|5|^
^^5||||R||X[CR]C|5|I||I[CR]R|6|^^^6||||R||X[CR]
C|6|I|[ETB]38[CR][LF]
Host 10:51:44,034 [ACK]
u411 10:51:44,346 [STX]2|I[CR]R|7|^^^7||||R||X[CR]C|7|I||I[CR]R|8
|^^^8||||R||X[CR]C|8|I||I[CR]R|9|^^^9||||R||X[C
R]C|9|I||I[CR]R|10|^^^10||||R||X[CR]C|10|I||I[C
R]R|11|^^^11||||R||X[CR]C|11|I||I[CR]R|12|^^^12
||||R||X[CR]C|12|I||I[CR]M|1|RR|83.49|[CR]M|2|R
R|80.23|[CR]M|3|RR|82.25|[CR]M|4|RR|78.72|[CR]M
|5|RR|81.64|[CR]M|[ETB]F8[CR][LF]
Host 10:51:44,346 [ACK]
u411 10:51:44,565 [STX]36|RR|81.78|[CR]M|7|RR|80.56|[CR]M|8|RR|75
.75|[CR]M|9|RR|79.73|[CR]M|10|RR|82.63|[CR]M|11
|RR|80.48|[CR]M|12|RR|79.14|[CR]M|13|RR|75.16|[
CR]M|14|RR|80.71|[CR]M|15|RR|73.00|[CR]M|16|RR|
0|[CR]L|1|[CR][ETX]05[CR][LF]
Host 10:51:44,565 [ACK]
u411 10:51:44,612 [EOT]

u411 10:45:58,677 [ENQ]
Host 10:45:58,677 [ACK]
u411 10:45:58,974 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
1||0^^^^CONTROL||R||||||X\Q|||20110315100924[CR
]R|1|^^^1|1.010|||||[CR]C|1|I||I[CR]R|2|^^^2|8|
||||[CR]C|2|I||I[CR]R|3|^^^3|NEG|||||[CR]C|3|I|
|I[CR]R|4|^^^4|NEG|||||[CR]C|4|I||I[CR]R|5|^^^5
|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG|||||[CR]C
|6|I||[ETB]CD[CR][LF]
Host 10:45:58,974 [ACK]
u411 10:45:59,208 [STX]2I[CR]R|7|^^^7|NEG|||||[CR]C|7|I||I[CR]R|8
|^^^8|NEG|||||[CR]C|8|I||I[CR]R|9|^^^9|NEG|||||
[CR]C|9|I||I[CR]R|10|^^^10|NEG|||||[CR]C|10|I||
Version 2.3 49 / 54
I[CR]R|11|^^^11|p.yel|||||[CR]C|11|I||I[CR]M|1|
RC|||ctrl|123456|[CR]L|1|[CR][ETX]E3[CR][LF]
Host 10:45:59,208 [ACK]
u411 10:45:59,255 [EOT]

u411 10:50:08,925 [ENQ]
Host 10:50:08,925 [ACK]
u411 10:50:09,222 [STX]1H|\^&|||8|||||||P|3.2.0.1001[CR]P|1[CR]O|
1||0^^^service^CONTROL||R||||||X\Q|||2011031510
0924[CR]R|1|^^^1|1.010|||||[CR]C|1|I||I[CR]R|2|
^^^2|8|||||[CR]C|2|I||I[CR]R|3|^^^3|NEG|||||[CR
|5|^^^5|NEG|||||[CR]C|5|I||I[CR]R|6|^^^6|NEG|||
||[CR][ETB]0C[CR][LF]
Host 10:50:09,222 [ACK]
|10|I||I[CR]R|11|^^^11|p.yel|||||[CR]C|11|I||I[
CR]M|1|RR|74.53|[CR]M|2|RR|73.39|[CR]M|3|RR|72.
|76.90|[CR]M|7|[ETB]0B[CR][LF]
Host 10:50:09,534 [ACK]
u411 10:50:09,753 [STX]3RR|78.11|[CR]M|8|RR|62.41|[CR]M|9|RR|77.3
3|[CR]M|10|RR|70.10|[CR]M|11|RR|74.08|[CR]M|12|
RR|73.60|[CR]M|13|RR|32.52|[CR]M|14|RR|21.44|[C
R]M|15|RR|05.43|[CR]M|16|RR|0|[CR]M|17|RC|||ctr
l|123456|[CR]L|1|[CR][ETX]9F[CR][LF]
Host 10:50:09,753 [ACK]
u411 10:50:09,800 [EOT]

ASTM Urisys 2400 protocol doesnt support worklist download
Version 2.3 50 / 54
Appendices - Appendix 1: ASCII-table
Appendices
6.3 Appendix 1: ASCII-table
Version 2.3 51 / 54
Appendices - Appendix 2: Definition of test codes and test numbers
(for ASTM plus protocol only)
6.4 Appendix 2: Definition of test codes and test numbers

(for ASTM plus protocol only)
Parameter Test code Test number

Specific Gravity SG 1
pH-value pH 2
Leukocytes LEU 3
Nitrite NIT 4
Protein PRO 5
Glucose GLU 6
Ketones KET 7
Urobilinogen UBG 8
Bilirubin BIL 9
Erythrocytes ERY 10
Compensation pad COM 11
Color COL 12
Clarity CLA 13

Sediment Parameter 1 Name 1. Sediment Parameter 51
Version 2.3 52 / 54
Appendices - Appendix 3: Printouts of control and sample results
6.5 Appendix 3: Printouts of control and sample results
Sample Result - Sample 1 Sample Result - Sample 2

with !-flag for pH
Version 2.3 53 / 54
Appendices - Appendix 3: Printouts of control and sample results
Sample Result - Sample 3 Sample Result - Sample 4

with *- and S-flag for pH with T-flag
and sediment data for ERY and LEU
Control Result
Version 2.3 54 / 54

Cobas 4u11 Host Interface Manual New Upload

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Roche Diagnostics International Ltd

Host Interface Manual

ROCHE DIAGNOSTICS INTERNATIONAL LTD MAKES NO REPRESENTATIONS OR

COPYRIGHT 2011, BY ROCHE DIAGNOSTICS INTERNATIONAL LTD. ALL RIGHTS

Version Date Software version Modifications

1.1 Document structure

1.3 Related documents

1.4 Used syntax and abbreviations

E-Mail address: global.gcs_rpd-urin@roche.com

Clarify queue: GSSCDIRIC

2.1 Overview cobas u 411 analyzer

2.2 Supported host protocols

The cobas u 411 analyzer supports the following host protocols:

2.3 Sample ID length

Additional flags for ASTM Urisys 2400 protocol only

3.1 Upload (cobas u 411 to host system)

ASTM Urisys 2400

ASTM Urisys 2400

Sample ID / Sequence number Name and lot ID

Sediment results - compensated raw data (optional)

compensated raw data (optional) Instrument ID / serial no.

Legend: Supported - not supported

ASTM plus protocol

ASTM Urisys 2400

3.2 Arbitrary units

ASTM Urisys 2400

3.3 Download data (host system to cobas u 411)

4 Host interface configuration

4.1 Communication settings

Configuration Specification Default

4.2 Host interface behaviour

Item Specification Default

4.3 Serial cable

Please see table below for wiring details:

cobas u 411 Host System

5 Error handling (behaviour of a failed transmission)

There are two different types of error handling:

Host receives wrong data:

6 Description of the supported protocols

6.1 ASTM plus protocol

Identification Name Description

Description Start of Frame Protocol End of Check- Carriage Line feed

6.1.2 Communication startup

A communication is initialized when the ASCII character ENQ

The host system (slave) acknowledges its readiness to

If the host system has positively acknowledged its readiness to

The host system confirms the frame reception with ACK.

The communication session is terminated with the ASCII

6.1.3 Record description

Message header record

No. Field Content Comment

Patient information record

No. Field Content Comment

No. Field Content Comment

16 .. 31 All further fields are not supported and not transmitted

Example patient sample:

Example control sample:

No. Field Content Comment

No. Field Content Comment

Read the chapter 2.4 Flags for detailed information!