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    F.Y.B.Sc. Sem II Practical I: Department of Zoology C.K.Thakur ACS College, New Panvel

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    Mithun Mejari
    1. The document provides instructions for three practical experiments to mount and observe different types of fish scales: placoid scales from cartilaginous fish like sharks, and cycloid and ctenoid scales from bony fish. 2. For placoid scales, shark skin is boiled in KOH and observed under a microscope, revealing spiny scales embedded in the dermis for protection. 3. For cycloid scales, a scale is plucked from a bony fish and observed, showing small, oval overlapping scales used to estimate age. 4. For ctenoid scales, a scale with spiny posterior margins is plucked and observed from a bony fish, also used to estimate age.

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    F.Y.B.Sc. Sem II Practical I: Department of Zoology C.K.Thakur ACS College, New Panvel

    Uploaded by

    Mithun Mejari
    658 views3 pages
    1. The document provides instructions for three practical experiments to mount and observe different types of fish scales: placoid scales from cartilaginous fish like sharks, and cycloid and ctenoid scales from bony fish. 2. For placoid scales, shark skin is boiled in KOH and observed under a microscope, revealing spiny scales embedded in the dermis for protection. 3. For cycloid scales, a scale is plucked from a bony fish and observed, showing small, oval overlapping scales used to estimate age. 4. For ctenoid scales, a scale with spiny posterior margins is plucked and observed from a bony fish, also used to estimate age.

    Original Title

    Scales of fishes (1)

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    1. The document provides instructions for three practical experiments to mount and observe different types of fish scales: placoid scales from cartilaginous fish like sharks, and cycloid and ctenoid scales from bony fish. 2. For placoid scales, shark skin is boiled in KOH and observed under a microscope, revealing spiny scales embedded in the dermis for protection. 3. For cycloid scales, a scale is plucked from a bony fish and observed, showing small, oval overlapping scales used to estimate age. 4. For ctenoid scales, a scale with spiny posterior margins is plucked and observed from a bony fish, also used to estimate age.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    658 views3 pages

    F.Y.B.Sc. Sem II Practical I: Department of Zoology C.K.Thakur ACS College, New Panvel

    Uploaded by

    Mithun Mejari
    1. The document provides instructions for three practical experiments to mount and observe different types of fish scales: placoid scales from cartilaginous fish like sharks, and cycloid and ctenoid scales from bony fish. 2. For placoid scales, shark skin is boiled in KOH and observed under a microscope, revealing spiny scales embedded in the dermis for protection. 3. For cycloid scales, a scale is plucked from a bony fish and observed, showing small, oval overlapping scales used to estimate age. 4. For ctenoid scales, a scale with spiny posterior margins is plucked and observed from a bony fish, also used to estimate age.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    F.Y.B.Sc.

    Sem II Practical I

    Date:
    MOUNTING OF SCALES OF FISH
    Aim: To mount and observe placoid scales of cartilaginous fish.

    Requirements: Shark skin, 10% KOH, test tubes, test tube holder, watch glass, slide,
    microscope.

    Method:
    1. Take a piece of skin of shark in a watch glass and remove attached muscles with
    the help of forceps.
    2. Transfer the skin into a test tube containing 2-3 ml 10% KOH. Boil for a few
    minutes.
    3. Allow the test tube to stand for a few minutes. Decant the supernatant leaving a
    few drops in the test tube. Pour this residual KOH into a watch glass.
    4. Place a drop from it onto a slide.
    5. Observe under low power of a compound microscope.
    Observation for Placoid scale:
    1. Each scale has a flat base plate embedded in the dermis.
    2. From the base plate of these primitive scales arise sharp, trident spines.
    3. These tough, microscopic scales cover the skin and are protective in function.
    4. They are found only in cartilaginous fishes (elasmobranchs) i.e., sharks and rays.
    5. Structurally they show inner core of pulp, middle layer of dentine and enamel
    covering and hence modification of the same develops teeth in elasmobranchs.

    Department of Zoology C.K.Thakur ACS College, New Panvel


    F.Y.B.Sc. Sem II Practical I

    Aim: To mount and observe cycloid scales of bony fish.

    Requirements: Sciaena, watch glass, slide, blunt forceps, dissecting and compound
    microscopes.

    Method:
    1. Pluck a scale from the body of the fish with forceps and rinse with water.
    2. Observe under the dissecting and low power of compound microscope.

    Observation for Cycloid scale:


    1. Each scale is small, oval-shaped and smooth with a uniform outer margin.
    2. It is made up of an inner layer of collagen fibres and an outer organic layer
    impregnated with calcium salts.
    3. Like the ctenoid scale, the cycloid scale also grows in size resulting in development of
    growth rings. These can be used to estimate the age of a fish.
    4. These scales overlap each other which gives them flexibility.
    5. They are found in bony fishes i.e., teleost.

    Department of Zoology C.K.Thakur ACS College, New Panvel


    F.Y.B.Sc. Sem II Practical I

    Aim: To mount ctenoid scales of bony fish

    Requirements: Sciaena (Dhoma)/Sole fish, watch glass, slide, flat, blunt forceps,
    dissecting and compound microscope.

    Method:
    1. Pluck a scale from the body of the fish preferably with blunt forceps and rinse with
    water.
    2. Observe under the dissecting and low power of compound microscope.

    Observation for Ctenoid scale:


    1. Each scale is oval shaped with a spiny posterior margin bearing spines called ctenii.
    2. It is made up of an inner layer of collagen fibres and an outer organic layer
    impregnated with calcium salts.
    3. These scales partially overlap thereby bringing about flexibility in the movement.
    4. As the fish grows the scale also grows resulting in development of incomplete growth
    rings.
    5. They can be used to estimate the age of a fish.
    6. They are found in teleost i.e., bony fishes

    Department of Zoology C.K.Thakur ACS College, New Panvel

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