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    Product Information: Safegreen ™ Loading Dye Catalog Number Packaging Size D012 1 ML Protocol

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    Product Information: Safegreen ™ Loading Dye Catalog Number Packaging Size D012 1 ML Protocol

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    koko
    18 views1 page
    d msds

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    D012

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    18 views1 page

    Product Information: Safegreen ™ Loading Dye Catalog Number Packaging Size D012 1 ML Protocol

    Uploaded by

    koko
    d msds

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    7040 Virginia Manor Road

    Beltsville, MD 20705, USA


    Web: www.abpbio.com;
    Email: info@abpbio.com

    Product Information
    SafeGreen™ Loading Dye Protocol
    1. Prepare molten agarose gel solution, cast the gel and allow
    Catalog Number Packaging Size it to solidify using your standard protocol. (Unnecessary to
    add any DNA stain reagent.)
    D012 1 mL 2. Mix the DNA samples and/or DNA ladder with SafeGreen
    Loading Dye at 5:1 ratio.
    Storage upon receipt: 3. Load samples and run the gels using your standard
    protocol.
     -20°C
    4. Image the stained gel with a transilluminator, or a
     Protect from light laser-based gel scanner using a long path green filter such as
    Ex/Em: 500/530 nm, bound to nucleic a SYBR Filter or GelStar filter.
    acid
     Description
    Product
    SafeGreen Loading Dye is a safe and highly sensitive
    fluorescent stain for detecting nucleic acids in agarose gel.
    This single stain gives high sensitivity detection of
    double-stranded or single-stranded DNA and RNA. The stain
    is simply mixed with DNA samples, and run the gels, providing
    a simple and fast protocol. SafeGreen Loading Dye is
    compatible with a standard 300 nm transilluminator, a 254 nm
    transilluminator, a blue-light transilluminator, or a gel reader
    equipped with visible light excitation such as a 488 nm
    laser-based gel scanner.
    SafeGreen Loading Dye is a ready-to-use solution. The stain
    is premixed with DNA samples and/or DNA ladder at 1:5 ratio
    before running the gel. For example, for every 5 L DNA
    samples, adding 1 L of stain reagent. One vial (1 mL) of stain
    reagent can be used to run at least 1000 DNA samples.
    Gel staining with SafeGreen Loading Dye is compatible with
    downstream applications such as gel extraction and cloning.
    SafeGreen Loading Dye is efficiently removed from DNA by
    phenol/chloroform extraction and ethanol precipitation.

    Spectral Characteristics

    SafeGreen Loading Dye

    Related Products
    Catalog No. Product
    D013 SafeRed™ Loading Dye, 1 mL

    Excitation (blue) and emission spectra (red) of SafeGreen


    bound to dsDNA in TBE buffer

    SafeGreen™ Loading Dye Page 1

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